Structure of PDB 7ohs Chain x Binding Site BS01

Receptor Information

>7ohs Chain x (length=267) Species: 559292 (Saccharomyces cerevisiae S288C)

NKLKRQEIFADIKHEKNKERHTMRRKRAKEERENPELREQRLKENVTQTI
ENTRVYEDDLMRYFNSNSNEPPKIFLTTNVNAKKSAYEFANILIEILPNV
TFVKRKFGYKLKEISDICIKRNFTDIVIINEDKKKVTGLTFIHLPEGPTF
YFKLSSFVEHGRPTSHIPELILNNFQTRLGQTVGRLFQSILPQNPDIEGR
QVITLHNQRDYIFFRRHRYVFKDNERVGLQELGPQFTLKLKRLQRGIKEE
TEWEHKPEMDKEKKKFY

Ligand information

>7ohs Chain 1 (length=1697)

guuugaccucaaaucagguaggaguacccgcugaacuuaagcauaucaau
aagcggaggaaaagaaaccaaccgggauugccuuaguaacggcgagugaa
gcggcaaaagcucaaauuugaaaucugguaccuucggugcccgaguugua
auuuggagagggcaacuuuggggccguuccuugucuauguuccuuggaac
aggacgucauagagggugagaaucccguguggcgaggagugcgguucuuu
guaaagugccuucgaagagucgaguuguuugggaaugcagcucuaagugg
gugguuccaucuaaagcuaaauauuggcgagagaccgauagcgaacaagu
acagugauggaaagaugaaaagaacuuugaaaagagagugaaaaaguacg
ugaaauuguugaaagggaagggcauuugaucagacaugguguuuugugcc
cucugcuccuuguggguaggggaaucucgcauuucacugggccagcauca
guuuugguggcaggauaaauccauaggaauguagcuugccucgguaagua
uuauagccugugggaauacugccagcugggacugaggacugcgacguaag
ucaaggaugcuggcauaaugguuauaugccgcccgucuugaaacacggac
caaggagucuaacgucuaugcgaguguuuggguguaaaacccauacgcgu
aaugaacgagguuggggccucgcaagaggugcacaaucgaccgauccuga
ggauggauuugguaagagcauagcuguugggacccgaaagauggugaaaa
ccaucuaguagcugguuccugccgaaguuucccucaggauagcagaagcu
cguaucaguuuuaugaguaagaaugaagagcuuuuagugggccauuuuug
guaagcagaacuggcgaugcgggaugaaccgaacguagaguuaaggugcc
ggaauacacgcucaucagaacaaaagguguuaguucaucuagacagccgg
acgguggccauggaagucggaauccgcuaaggaguguguaacaacucacc
ggccgaaugaacuagcccugaggauggcgcucaagcguguuaccuauacu
cuaccgucaggguugauaugaugcccugacgaguaggcaggcguggaggu
cagugacgaagccuagaccguaaggucgggucgaacggccucuagugcag
aucuuggugguaguagcagcgaaaccacagccaagggaacgggcuuggca
gaaucagcggggcagucaagcggcgacauugcuaucauaccgaagcagaa
uucgguaagcgucuacugaugaauguuaccgcaauaguaauugaacuuag
uacgagaggaacaguucauucggauaauugguuuuugcggcugucugauc
aggcauugccgcgaagcuaccauccgcuggauuauggcugaacgccucua
agucagaauccaugcuagaacgcggugaugcuccacacagauggauacga
auaaggcguccuuguggcgucgcugaaccauagcaggcuagcaacggugc
acuuggcggaaaggccuugggugcuugcuggcgaauugcaaugcgugggg
auaaaucauuuguauacgacuuagauguacaacgggguauuguaagcagu
agaguagcguuacgcugcugagauuaagccuuuguugucugauuugu

..........................<<<<<<..................
.>>>>>.>.........<<....>>..<<<<<......<<.....>>...
..>>>>>..<<<..<........<<.<<<<<<...>>>>>>.>>......
..>....<<<<<<<.<<<<<<<<<<<<<<<<<<<<.....<<<<......
>>>>.((......<<<.....))>>>....>>>>>>>>..>>>>>>>>..
..>>>>>>>>>>>........<<<<<<<........>>>>>>>.....<<
<<<....>>>>>..>>>.................................
.<<....>>...............<<<......>>>..............
....................<<<<<.<<<<<<.....<<<<...<<<<.<
<<<<<<<<.....>>>>>>>>>.....>>>>...>>>>..<<<<<<<.<<
<<<<<<<<<<<<<......<<<<<<<<......<<<<<......>>>>>.
......>>>>>>>>....>>>>>.>>>>>>>>>>........<<<....>
>>.....>>>>>>>....>>>>>>.>>>>>..<<<<........>>>><<
<<<<....<<<<<<.<<<<<<..<<<...<<<<.....>>>>...>>>..
.........<<<<<..<<<<<....>>>>>...>>>>>...<<..<<...
....>>...>>......>>>>>>.>>>>>>..<<.<...<<<<<<....>
>>>>>......>.>>..<<<<<<......<<......>>..<<<<<<<<<
.....<<.<<<<<<..>>>>.>>>>...>>>>>>>.>>..<<<<.<.<..
.......>.>.>>>>....<<<......>>>...<<<<<<<..<<<<(((
.....<<<<<<......<......<<<.<<<<<<<<<<.......<<<<<
.<<<...<<.........>>...>>>.........<<......>>...>>
>>>...>>>>>>>>>>>>>......))).>....>>>>>>.>>>>..>>>
>>>><<<<<<<<<.........>>>>>>>>>....>>>>>><..<<<<<<
<.<<......<<<<.<<<<....>>>>>>>>...>>>>>>>>>...>...
..>>>>>><<.....<<.>>....>>....<<<<<........>>>>>..
...<<<......<<<....<<.>>....>>>....<...<<<<<......
>>>>>.....>.....>>>.....<<<<<<<<..<<<<.<.<<<<<<...
..<....>....>>>>>>.><<<<<...<.......<<<<<.........
.......>>>>>......>..>>>>>.<<<<...<<<<<...........
>>>>>...>>>>.>>>>....>>>>>>>>.<<<<<<......<<......
....<<<<<.<....>>>>>>......>>.......<<<<<<...<<<<<
....<<<......>>>....>>>>>.>>>>>>............>>>>>>
.<<<<<<.<.............>.....<<<<<.<<<.<<<.....<<<.
..............>>>....>>>....>>>..>>>>>..>>>>>>.

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7ohs Analysis of subunit folding contribution of three yeast large ribosomal subunit proteins required for stabilisation and processing of intermediate nuclear rRNA precursors.
• Resolution: 4.38 Å
• Binding residue (original residue number in PDB): N11 K12 L13 K14 Q16 F19 K23 H24 K28 H31 R34 R48 N55 V56 T57 Q58 T59 I60 N102 A103 K104 K105 Y108 K125 F128 K155 R189 K283 P284 K290 K291
• Binding residue (residue number reindexed from 1): N1 K2 L3 K4 Q6 F9 K13 H14 K18 H21 R24 R38 N45 V46 T47 Q48 T49 I50 N81 A82 K83 K84 Y87 K104 F107 K134 R162 K256 P257 K263 K264

Gene Ontology

Molecular Function

• GO:0005515 protein binding
• GO:0019843 rRNA binding
• GO:0042134 rRNA primary transcript binding

Biological Process

• GO:0000055 ribosomal large subunit export from nucleus
• GO:0000460 maturation of 5.8S rRNA
• GO:0000463 maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
• GO:0000466 maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
• GO:0000470 maturation of LSU-rRNA
• GO:0006364 rRNA processing
• GO:0042254 ribosome biogenesis

Cellular Component

• GO:0005634 nucleus
• GO:0005730 nucleolus
• GO:0030687 preribosome, large subunit precursor

External links

• PDB: RCSB:7ohs, PDBe:7ohs, PDBj:7ohs
• PDBsum: 7ohs
• PubMed: 34813592
• UniProt: P38805|RPF1_YEAST Ribosome production factor 1 (Gene Name=RPF1)