Structure of PDB 7nad Chain x Binding Site BS01

Receptor Information
>7nad Chain x (length=539) Species: 1247190 (Saccharomyces cerevisiae BY4741) [Search protein sequence] [Download receptor structure] [Download structure with residue number starting from 1] [View receptor structure]
SLEWDNLGFSLLPWIRTGLDVMGFETMTPVQASTIPMLAGNKDVVVDSVT
GSGKTAAFVIPVLEKVVKEEANTSKFKKAHFHSLIIAPTRELSRQIESVV
LSFLEHYPSDLFPIKCQLLVGTNEATVRDDVSNFLRNRPQILIGTPGRVL
DFLQMPAVKTSACSMVVMDEADRLLDMSFIKDTEKILRLLPKQRRTGLFS
ATMRSAGSDIFKTGLRNPVRITVNSSSLKLNYCVVNPAEKLQLLVSILNN
YKFKKCIVYFPTCVSVSYFYSFIQYLGKRNILVNEVEIFSLHGKLQTSAR
TKTLTAFTDSNSVLFTTDVAARGIDIPDVDLVIQLDPPTNTDMFMHRCGR
TGRANRVGKAITFLNEGREEDFIPFMQVKNVELEELDLEVKGITANFYED
FRNWILEDRDRFDKGVKAYVAFIKYYSNHSATSIFRLQSLDYVGIAKLYG
LFRLPRMPEITKYLNWLVDPPVNMDEYKYKDKKREKERQETLKNISLIND
KKKLKSELKKKNLAWSDKTLTKERKLERKEKMSLKRKAI
Ligand information
>7nad Chain 1 (length=697) [Search RNA sequence] [Download ligand structure] [Download structure with residue number starting from 1] [View ligand structure]
augccugaauagggugaagccagaggaaacucugguggaggcucgcgaau
uuggguauaguagcaaauauucaaaugagaacuuugaagacugaaguggg
gaaagguuccacgucaacagcaguuggacguggguuagucgauccuaaga
gaugguuucaaaggccugacaggccaccaucgaaagggaauccgguuaag
auuccggaaccuggauauggauucuucacgguaacguaacugaaugugga
gacgucggcgcgagcccugggaggaguuaucuuuucuucuuaacagcuua
ucaccccggaauugguuuauccggagauggggucuuauggcuggaagagg
ccagcaccuuugcuggcuccggugcgcuugugacggcccgugaaaaucca
caggaaggaauaguuuucaugccaggucguacugucuccaaggugaacag
ccucuaguugauagaagauaagggaagucgguccguaacuucgggauaag
gauuggcucuaagggucggguagugagggccuuggucacggccuuggcuu
gcuacaauuaacgaucaacuuagaacugguacggacaaggggaaucugac
uguuaacgagauucccacugucccuaucuacuaucuagcgaggcugucug
aucaggcauugcguaagcaguagaguagccguuacgaucugcugaga
<<<<<<<<...<<<....<<<<<<<....>>>>>>>.....>>>......
>>>>>>>>....<<.....<<<<<<.......>>>>>><<<<.....<<<
<<.<<...<<<<<<...<<.....>>.>>>>>>....((<<<<<<...<<
............<<<<<<.>>>>>>....>>....>>...<<<<<.....
...>>>>>))<<<<.<<<<<<......<<<....>>>.......<<<<<<
...<<<<.<<<<<<<.<<<<<<<<<<..(((..>>>>>><...<<<<<..
..<<<<<<....<<<.....>>>....>>>>>>.....>>>>>....><<
<<<<<......>>>>>>>>>>>...>>>>>>>.>>>>.........>>>>
>>............>>.>>>>>>>>>>>>..>>.>>>>>.<<<<.....>
>>>..>>>>..<<<<.<<<<.<<........<<<<<<.<<<<........
.............<<<<<.<<<<<<<<..<<..<<<<..>>>>..>>>>>
>>>>>......>>>>>......>>>...>>>>>>>....<<<<<<<<<..
......>>.>>>>>>>.....>>.>>>>.....>>>>>>...<<<<<<<)
)).>>>>>..>>....<<<<....<<.......>>...>>>>.....
Receptor-Ligand Complex Structure
Global viewLocal viewStructure summary

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PDB7nad Sequence-specific remodeling of a topologically complex RNP substrate by Spb4.
Resolution3.04 Å
Binding residue
(original residue number in PDB)
P91 R93 G124 T125 T148 G150 R151 D154 R176 F182 E187 K195 K215 P269 T270 C271 Y278 F297 S298 G301 L303 R308 K310 T311 T327 D328 V329 V430 A431 K434 Y435 R463 L464 P465 R466 M467 P468 E469 K504 K522 K523 K524 K526 N533 L534 W536 S537 D538 R545 K546 E548 R549
Binding residue
(residue number reindexed from 1)
P88 R90 G121 T122 T145 G147 R148 D151 R173 F179 E184 K192 K212 P261 T262 C263 Y270 F289 S290 G293 L295 R300 K302 T303 T317 D318 V319 V420 A421 K424 Y425 R453 L454 P455 R456 M457 P458 E459 K483 K501 K502 K503 K505 N512 L513 W515 S516 D517 R524 K525 E527 R528
Enzymatic activity
Enzyme Commision number 3.6.4.13: RNA helicase.
Gene Ontology
Molecular Function
GO:0003676 nucleic acid binding
GO:0003723 RNA binding
GO:0003724 RNA helicase activity
GO:0004386 helicase activity
GO:0005515 protein binding
GO:0005524 ATP binding
GO:0016787 hydrolase activity
GO:0016887 ATP hydrolysis activity
Biological Process
GO:0000027 ribosomal large subunit assembly
GO:0000470 maturation of LSU-rRNA
GO:0006364 rRNA processing
GO:0006396 RNA processing
GO:0042254 ribosome biogenesis
GO:1902626 assembly of large subunit precursor of preribosome
Cellular Component
GO:0005634 nucleus
GO:0005654 nucleoplasm
GO:0005730 nucleolus
GO:0030686 90S preribosome
GO:0030687 preribosome, large subunit precursor

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Molecular Function

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Biological Process

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Cellular Component
External links
PDB RCSB:7nad, PDBe:7nad, PDBj:7nad
PDBsum7nad
PubMed36482249
UniProtP25808|SPB4_YEAST ATP-dependent rRNA helicase SPB4 (Gene Name=SPB4)

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