Structure of PDB 8wif Chain w Binding Site BS01

Receptor Information
>8wif Chain w (length=126) Species: 246196 (Mycolicibacterium smegmatis MC2 155) [Search protein sequence] [Download receptor structure] [Download structure with residue number starting from 1] [View receptor structure]
MDVDVSTDGELPGAAEYAREKIGRLSRRAHRPVLHARVRLTRHGDPAVER
PVIAQANLDVNGRQVRAQVEGVNAREAVDRLEARLRSRLERIAEHWEARR
GGVPAEAGREWRHESEPARRPGYFPR
Ligand information
>8wif Chain a (length=1517) [Search RNA sequence] [Download ligand structure] [Download structure with residue number starting from 1] [View ligand structure]
uuuggagaguuugauccuggcucaggacgaacgcuggcggcgugcuuaac
acaugcaagucgaacggaaaggcccuuucggggguacucgaguggcgaac
gggugaguaacacgugggugaucugcccugcacuuugggauaagccuggg
aaacugggucuaauaccgaauacacccugcuggucgcauggccugguagg
ggaaagcuuuugcggugugggaugggcccgcggccuaucagcuuguuggu
ggggugauggccuaccaaggcgacgacggguagccggccugagaggguga
ccggccacacugggacugagauacggcccagacuccuacgggaggcagca
guggggaauauugcacaaugggcgcaagccugaugcagcgacgccgcgug
agggaugacggccuucggguuguaaaccucuuucagcacagacgaagcgc
aagugacgguaugugcagaagaaggaccggccaacuacgugccagcagcc
gcgguaauacguaggguccgagcguuguccggaauuacugggcguaaaga
gcucguaggugguuugucgcguuguucgugaaaacucacagcuuaacugu
gggcgugcgggcgauacgggcagacuagaguacugcaggggagacuggaa
uuccugguguagcgguggaaugcgcagauaucaggaggaacaccgguggc
gaaggcgggucucugggcaguaacugacgcugaggagcgaaagcgugggg
agcgaacaggauuagauacccugguaguccacgccguaaacgguggguac
uagguguggguuuccuuccuugggauccgugccguagcuaacgcauuaag
uaccccgccuggggaguacggccgcaaggcuaaaacucaaaggaauugac
gggggcccgcacaagcggcggagcauguggauuaauucgaugcaacgcga
agaaccuuaccuggguuugacaugcacaggacgccggcagagaugucggu
ucccuuguggccugugugcagguggugcauggcugucgucagcucguguc
gugagauguuggguuaagucccgcaacgagcgcaacccuugucucauguu
gccagcacguuaugguggggacucgugagagacugccggggucaacucgg
aggaagguggggaugacgucaagucaucaugccccuuauguccagggcuu
cacacaugcuacaauggccgguacaaagggcugcgaugccgugaggugga
gcgaauccuuucaaagccggucucaguucggaucggggucugcaacucga
ccccgugaagucggagucgcuaguaaucgcagaucagcaacgcugcggug
aauacguucccgggccuuguacacaccgcccgucacgucaugaaagucgg
uaacacccgaagccgguggccuaacccuuguggagggagccgucgaaggu
gggaucggcgauugggacgaagucguaacaagguagccguaccggaaggu
gcggcuggaucaccucc
......<<<<..[.((((.>>>>.<<<<.<<<<<..<<<<<<<<.....<
<<.<<<..<<<..<<.<..<<<<<<<....>>>>>.>>>..>>>>>....
..<<........<<<<<<<..<<...<<<<<<<<<<<<.....<<<<<<.
...>>>>>>......>>>>.....<<<<<<<<<<<<..>>>>>.>>>>>>
>....<<....>>>>>>>>>>..>>>>>>>>>.<<<....<<<..<<<<<
<<<.......>>>>>>>>>>>......>>>..<<<<<<<<....>>>>..
.>>>>.>>.<<<<<.<.........>>>>>>.<<<<....>>>>...>>>
>>>.........<<<...<<<<<....>>>>.>>>>..>>.>>>>>>..<
<<<......<<<<....>>>>.....>>>>...<.<<<<<......<<..
..>>.......>>>>>....>..<<<<<(((...<<<<<.....<<.)))
>>.......>>>>>>>>>>..>>>>>>>>>..........<<<((.....
<<<<...<<<.<<<<<<<.<<<<<<<<<<.....<<<<<<<.....>>>>
>>>...>>>>>>>..>>>>>>>>>>...<<<<<<<<...<<<<<<<....
<<<<<<<<<..<<<......>>>.....>>>>>>>>>...........<<
....>>.>>>>>>>..>>>>>.>>>...>>>...>>>>....<<<<<<..
.<<...<<<<.<.....>.>>>>...>>>>>>>>..........<<<<<<
..<<<<<<<<<<<<.......>>>>>>>>>>>>...<<..))>>.....>
>>>>>.>>>.<<<......<<<<....>>>>....>>>..)))).]<<<<
<.<<<<<<<.<<.<<<<<<..<<<<<<<<<<......<<........>>.
.........<<<<<<<......<<<<<<<...<<<<<<<....>>>>>>>
.<<.....>>.>>>>>>>.<<<.<<<..<<<<<<<......<<<<<<<<<
....>>>..<<<<......>>>>..>>>>>>.....<<<<.<<<<<<...
.<<..<<<......>>>>>.....>>>>>>.....<<<<<.....>>>>>
........>>>>.......>.>>>...>>>>>>>>>...>>>>>>>...>
>.>>>>>>>>.....<<<<<<<.....<<<..<<..<<<<....>>>>..
>>....>>>......>>>>>>>......<....<<<<<<<........>>
>>>>>....>.....>>>>>>....<<<<<<<..........>>>>>>>.
.....>>...>>>>>>>>>>.>>....<..<<.<.<<<<.<<<..<<<<<
<..<<<<....<.<<<<..<<<..<<.....>>.>>>.>>>>.>...>>>
>..>>>>>>..>>>.>>>>..>.>>...>.....<<<<<<<<<....>>>
>>>>>>...........
Receptor-Ligand Complex Structure
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PDB8wif Cryo- EM structure of the mycobacterial 70S ribosome in complex with ribosome hibernation promotion factor RafH.
Resolution2.9 Å
Binding residue
(original residue number in PDB)
S6 T7 K21 R27 R28 H30 R37 R39 D45 A47 Q55 G62 R66 A67 Q68 R75 E76 D79 R80 R84 R86 R91 W96 E97 A98 R100 G101 E110 W111 R112 H113 E114 A118 R120 P121 F124 P125
Binding residue
(residue number reindexed from 1)
S6 T7 K21 R27 R28 H30 R37 R39 D45 A47 Q55 G62 R66 A67 Q68 R75 E76 D79 R80 R84 R86 R91 W96 E97 A98 R100 G101 E110 W111 R112 H113 E114 A118 R120 P121 F124 P125
Enzymatic activity
Enzyme Commision number ?
Gene Ontology
Molecular Function
GO:0043024 ribosomal small subunit binding
Biological Process
GO:0006417 regulation of translation
GO:0044238 primary metabolic process
GO:0045900 negative regulation of translational elongation
Cellular Component
GO:0022627 cytosolic small ribosomal subunit

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Molecular Function

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Biological Process

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Cellular Component
External links
PDB RCSB:8wif, PDBe:8wif, PDBj:8wif
PDBsum8wif
PubMed38245551
UniProtA0QZ86

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