Structure of PDB 7nad Chain w Binding Site BS01

Receptor Information
>7nad Chain w (length=328) Species: 1247190 (Saccharomyces cerevisiae BY4741) [Search protein sequence] [Download receptor structure] [Download structure with residue number starting from 1] [View receptor structure]
KNSKGRLDRYYARSSFKIIQINEKYGHFLLHDGAPNVGLGWVQDAFTQSQ
LTLQALKTFVTKIFRSKDYNKLIWVFQQLFEKVEATKPPASRNVSAEIFV
VCKGFKAPKRLDPRLLDPKEVFEELPDEKKVRKRQGYETEEEQIEKDLQG
LQEKQRLNVKRERRRKNEMKQKELQRMQMNMESLFNLKTAEKTGILNDLA
KGKKRMIFTMIKDKDSAADADDLESELNAMYSDYKTRRSERDAKFRAKQA
RGAITNLISKLKGQEGDHKLSSKARMIFNDPIFNNVEPFDSDYDSEEEKN
QTKKEKHSRDENLPDWFLEDAMNARPIK
Ligand information
>7nad Chain 1 (length=697) [Search RNA sequence] [Download ligand structure] [Download structure with residue number starting from 1] [View ligand structure]
augccugaauagggugaagccagaggaaacucugguggaggcucgcgaau
uuggguauaguagcaaauauucaaaugagaacuuugaagacugaaguggg
gaaagguuccacgucaacagcaguuggacguggguuagucgauccuaaga
gaugguuucaaaggccugacaggccaccaucgaaagggaauccgguuaag
auuccggaaccuggauauggauucuucacgguaacguaacugaaugugga
gacgucggcgcgagcccugggaggaguuaucuuuucuucuuaacagcuua
ucaccccggaauugguuuauccggagauggggucuuauggcuggaagagg
ccagcaccuuugcuggcuccggugcgcuugugacggcccgugaaaaucca
caggaaggaauaguuuucaugccaggucguacugucuccaaggugaacag
ccucuaguugauagaagauaagggaagucgguccguaacuucgggauaag
gauuggcucuaagggucggguagugagggccuuggucacggccuuggcuu
gcuacaauuaacgaucaacuuagaacugguacggacaaggggaaucugac
uguuaacgagauucccacugucccuaucuacuaucuagcgaggcugucug
aucaggcauugcguaagcaguagaguagccguuacgaucugcugaga
<<<<<<<<...<<<....<<<<<<<....>>>>>>>.....>>>......
>>>>>>>>....<<.....<<<<<<.......>>>>>><<<<.....<<<
<<.<<...<<<<<<...<<.....>>.>>>>>>....((<<<<<<...<<
............<<<<<<.>>>>>>....>>....>>...<<<<<.....
...>>>>>))<<<<.<<<<<<......<<<....>>>.......<<<<<<
...<<<<.<<<<<<<.<<<<<<<<<<..(((..>>>>>><...<<<<<..
..<<<<<<....<<<.....>>>....>>>>>>.....>>>>>....><<
<<<<<......>>>>>>>>>>>...>>>>>>>.>>>>.........>>>>
>>............>>.>>>>>>>>>>>>..>>.>>>>>.<<<<.....>
>>>..>>>>..<<<<.<<<<.<<........<<<<<<.<<<<........
.............<<<<<.<<<<<<<<..<<..<<<<..>>>>..>>>>>
>>>>>......>>>>>......>>>...>>>>>>>....<<<<<<<<<..
......>>.>>>>>>>.....>>.>>>>.....>>>>>>...<<<<<<<)
)).>>>>>..>>....<<<<....<<.......>>...>>>>.....
Receptor-Ligand Complex Structure
Global viewLocal viewStructure summary

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PDB7nad Sequence-specific remodeling of a topologically complex RNP substrate by Spb4.
Resolution3.04 Å
Binding residue
(original residue number in PDB)		K39 R162 S163 T183 K184 N190 V191 A193 V240 R241 R243 R369 R372 R373 R384 Y473 R476 R480
Binding residue
(residue number reindexed from 1)		K24 R65 S66 T86 K87 N93 V94 A96 V131 R132 R134 R161 R164 R165 R176 Y234 R237 R241
Enzymatic activity
Enzyme Commision number 2.1.1.167: 27S pre-rRNA (guanosine(2922)-2'-O)-methyltransferase.
Gene Ontology
Molecular Function
GO:0005515 protein binding
GO:0008168 methyltransferase activity
GO:0008649 rRNA methyltransferase activity
GO:0008650 rRNA (uridine-2'-O-)-methyltransferase activity
GO:0016435 rRNA (guanine) methyltransferase activity
GO:0070039 rRNA (guanosine-2'-O-)-methyltransferase activity
Biological Process
GO:0000451 rRNA 2'-O-methylation
GO:0000463 maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
GO:0000466 maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
GO:0001510 RNA methylation
GO:0006364 rRNA processing
GO:0031167 rRNA methylation
GO:0032259 methylation
GO:0042254 ribosome biogenesis
Cellular Component
GO:0005634 nucleus
GO:0005730 nucleolus
GO:0030687 preribosome, large subunit precursor

View graph for
Molecular Function
View graph for
Biological Process
View graph for
Cellular Component
External links
PDB RCSB:7nad, PDBe:7nad, PDBj:7nad
PDBsum7nad
PubMed36482249
UniProtP25582|SPB1_YEAST 27S pre-rRNA (guanosine(2922)-2'-O)-methyltransferase (Gene Name=SPB1)

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