Structure of PDB 8uu7 Chain v Binding Site BS01

Receptor Information

>8uu7 Chain v (length=416) Species: 169963 (Listeria monocytogenes EGD-e)

KKVLIVGISQKQKDFDYSMEELANLAAANNMEVVGEIRQNIDRENRATYV
GKGKVDEIKGLAEMQDARLIIFNDELSPSQIRNLEEALELDVMDRTGLIL
AIFANRAKTKEAQLQVQIAKLQYELPRIFGQGEDMDQQSGKGGLSNRGSG
EKKIETDRRTIKHQIRHLQKELDMLVDDREVRRRKRKKNEIPVVSLVGYT
NAGKSTTMNGLVRAYSETADKQVFEKDMLFATLETSVREIVLPDNKQFLL
TDTVGFVSKLPHQLVKAFRSTLEEARDADLLIHVVDYSDPHYKTMMKTTE
ETLKVVGVEDVPVIYAYNKADLLEDEMYPKQTGNTIIFSAREEESLEFLT
EVIRKELFASYEKATFLIPFEAGQVVAYLNEHADILETEYLENGTQIVAE
VSPADLQKLAEYQVAE

Ligand information

>8uu7 Chain A (length=2909)

guuaaguuagaaagggcgcacgguggaugccuuggcacuaggagccgaag
aaggacgggacuaacaccgauaugcuuuggggagcuguacguaagcguug
auccagagauuuccgaaugggggaacccacuaucuuuagucggauaguau
ccuuacgugaauacauagcgugaggaaggcagacccagggaacugaaaca
ucuaaguaccuggaggaagagaaagaaaaaucgauuuccugaguagcggc
gagcgaaacggaaagagcccaaaccaagaagcuugcuucuugggguugua
ggacacucuauacggaguuacaaaagaaaguuauaaaugaagcggucugg
aaaggcccgccaaagacgguaacagcccgguaguugaaauggcuuucccu
ccagaguggauccugaguacgacggaacacgugaaauuccgucggaaucc
gggaggaccaucucccaaggcuaaauacucccuagugaccgauagugaac
caguaccgugagggaaaggugaaaagcaccccggaaggggagugaaacag
uuccugaaaccgugugccuacaaguaguuagagcccguuaaugggugaua
gcgugccuuuuguagaaugaaccggcgaguuacgauuuguugcaagguua
agcggaaaaagcggagccguagcgaaagcgagucugaauagggcgcauaa
guaacaggucguagacccgaaaccaggugaucuacccauguccaggauga
agguaagguaauacuuacuggagguccgaacccacgcacguugaaaagug
cggggaugagguguggguagcggagaaauuccaaucgaacuuggagauag
cugguucucuccgaaauagcuuuagggcuagccucgagguaaagagucau
ggagguagagcacuguuuggacuaggggccgguuaccgaauucagauaaa
cuccgaaugccauguacuuauacucgggagucagacugcgagugauaaga
uccguagucgaaagggaaacagcccagaccaccaguuaagguccccaaau
auauguuaaguggaaaaggaugugggguugcuuagacaaccaggauguug
gcuuagaagcagccaccauugaaagagugcguaauagcucacuggucgag
ugaccccgcgccgaaaauguaccggggcuaaacauauuaccgaaacugug
gaugaaccuagguucgugguaggagagcguucuaagggcggugaagucag
accggaaggacugguggagcgcuuagaagugagaaugccgguaugaguag
cgaaagaagggugagaaucccuuccaccgaauaucuaagguuuccugagg
aaggcucguccgcucaggguuagucgggaccuaagccgaggccgauaggc
guaggcgauggacaacagguagagauuccuguaccagugcuaauuguuua
accgauggggugacacagaaggauagggaaucgcacgaauggaaaugugc
guccaagcagugagugugagaaguaggcaaauccgcuucuugcgaagcau
gagcugugauggggaaggguacggaaguuccugauuucacgcugucaaga
aaagccucuaggaagaguaguacugcccguaccgcaaaccgacacaggua
gaugaggagagaauccuaaggugagcgagagaacucucguuaaggaacuc
ggcaaaaugaccccguaacuucgggagaaggggugcucuauuagggugca
agcccgagagagccgcagugaauaggcccaggcgacuguuuagcaaaaac
acaggucucugcaaaaccguaaggugacguauaggggcugacgccugccc
ggugcuggaagguuaagaggagugcuuagcuucggcgaagguacgaauug
aagccccaguaaacggcggccguaacuauaacgguccuaagguagcgaaa
uuccuugucggguaaguuccgacccgcacgaaaggcgcaacgaucugggc
acugucucaacgagagacucggugaaauuauaguaccugugaagaugcag
guuacccgcgacaggacggaaagaccccguggagcuuuacugcaaccuga
uauggaauguuuguaccgcuuguacaggauagguaggagccgaagagacg
ugugcgcuagcauacgaggaggcaauggugggauacuacccuggcuguau
gaccauucuaacccgccacgcuaagcgcguggggagacagugucaggugg
gcaguuugacuggggcggucgccuccuaaagaguaacggaggcgcccaaa
gguucccucagaauggauggaaaucauucgcagaguguaaaggcacaagg
gagcuugacugcgagacugacaagucgagcagggacgaaagucgggcuua
gugauccggugguuccgcauggaagggccaucgcucaacggauaaaagcu
accccggggauaacaggcuuaucucccccaagaguccacaucgacgggga
gguuuggcaccucgaugucggcucgucgcauccuggggcuguagucgguc
ccaaggguugggcuguucgcccauuaaagcggcacgcgagcuggguucag
aacgucgugagacaguucggucccuauccgucgcgggcgcaggaaauuug
agaggagcuguccuuaguacgagaggaccgggauggacacaccgcuggug
uaccaguuguuccgccaggagcaucgcuggguagcuauguguggcaggga
uaaacgcugaaagcaucuaagcgugaagccccccucaagaugagauuucc
cauuucuucggaaaguaagaucccugaaagaugaucagguagauagguuu
ggaguggaaguguagcgauacauggagcggacaaauacuaaucgaucgag
gacuuaacc

<<<<<<<......<<<<<<<<<<<....{[[..[[<<<<<<<[[<<....
..<<<<<<.((..((.>>>.....<<<<<<<..<<<))..)).>>>....
.>>>>>>>...>>>....<<<....>>><<<<<<<......>>>>>>>.<
<<<<<<<<.........>>>>>>>>>.>>....<<<<<<.....<.....
.>......>>>>>.>.........<<....>>..<<<<<......<<...
..>>.....>>>>>..(((....<<<<<<<<....>>>>>>>>......<
<<<<<<<<<....<<<<........<<<<<<<<<.<<<.....<<<<...
...>>>>.((......<<<.....))>>>...>>>...>>>>>>>>>.>>
>>>>>>>>..>>>>.....<<<<<<<<.........>>>>>>>>......
<<<<<..<<<<>>>>>..)))......]].>>>>>>>.]]..........
.....<<....>>....]].........<<<<....>>>>..........
...}....>>>>>>>>>>>.......<<<<..<<<<......>>>>..>>
>>...<.<........>.>.<<<<<<....<<<<<<<<<<<<<..<<<..
.<<.......>>...>>>...<<....>>..<<<<.....>>>>......
>>>>>>>>>>>>>..<<.<..<<<<<<<.....<<<<<<<<<..<<<...
.<<<<<<......>>>>>>.....>>>...<<<.<<<<<........>>>
>>>>>....>>>>>>>>>...<<<.....>>>......>>>>>>...>..
>.>>..<<<<<<....<<<<<....>>>>><<<<<<<<.....<<<<<<<
<<.....<<<...<<<<<<<<...<<.<<..>>..>>...>>>>>>>>..
>>.>.....>>>>>.>>>>...>>>>>>.>>..<<<<<<<.<<......>
>.>>>>>>>....<<<......>>>...<<<<.<<<...<<<(((.....
<<<<<<...........<<..<<<<<<<<<........<<<<<.<<...<
<<<.......>>>>...>>.........<<......>>...>>>>>....
>>>>>>>>>.>>...........))).....>>>>>>.>>>...>>>>>>
><<<<<<<..>>>>>>>....>>>>>><.<<<<<<<.<<......<<<<<
.<<....>>.>>>>>...>>.>>>>>>>..>.....>>>>>><......<
<....<<<<<<.......>>>>>><<<<....<<<<<.<<...<<<<<<.
..<<.....>>.>>>>>>....((<<<<.<<...<<<...<<<....>>>
...>>>...>>...<<<<<........>>>>>))<<<<<<<<.<<.....
.<<...<<<<<...<<<<..<<<..<<<<<.<<<<<...(((....>>>>
>.<<..<<<<<..<<<<<<<<<<<.<<.....>>>>>>>>>.....>>>>
..>>>>>...>>....<....>.....>>>>>...>>>...>>>>.....
...>>>>>..>>..>>.>>>>>>>>>>>>..>>.....<<......>>>>
>>>.<<<<.....>>>>..>>>>..<<<<....<<<<<<<<.<<......
...<.....<<<<<....<<..>>.....>>>>><<<<<....<<<<...
.>>>>...>>>>>............<<<<<<<<<..<<<.........>>
>..<<<<<<<.....<<<....>>>.......>>>>>>>..<<<<.<<<.
.<<<<<<<..<<<...<<...<<<<<<.<<....>>..>>>>>>...>>.
..>>>>>>>>..>>>>><<<<<<.......>>>>>>........<.....
......<<<<<.......>>>>>.....>....>>>>...>>.>>>>>>>
..>..>>.>>>>>>>>.>>>>>>.....>.....<<<<<........>>>
>>..<<<<<<<<.(((.[[......<<<<.[[<<<<..<<<<<.<<<<<<
<<<.<<<<<.<<<<<<.<<<...........<<<<<..<<<.......<<
<<<<<....>>>>>>>....>>>............>>>>>..>>>.>>>>
>>.>>>>>....<<.<<<<<<.....>>>>>>>>.....>>>>>>>>>..
>>>>>..<<<<.....>>>><<<<<<...........>>>>>>..<<...
<<<<<<<...<<<<<<.......>>>>>>.....<<.[.(((>>....>>
>>>>>...<<<<..<<<<....>>>>..>>>>.<<<....>>>.>>)))]
......<<<<<<<.((..>>>>..))>>>>>>>.............>>>>
..]]>>>>.......<<<<.<.<<<<<<...<<.......>>...>>>>>
>...>>>>.>.......<<<<<.<<<<<<..<<<<<<<<<......>>>>
>>..>>>.<<<<<.....>>>>>.....>>>>>..>...>>>.>>....<
<<<<<<....>>>.>>>>....]]..)))>>>>>>>>....<<<<<<<..
.<<<<..<<<<<<<...............>>>>>>>.<<<<<...<<<<.
..<<<<<<<<<<<.)))>>>>>>..>>>>>...>>>>.>>>>>...<<<.
...<<<<<...........>>>>>....>>>.>>>>.......>>>>>>>
..<<<<<..>>>>>....<<<<<<<<<.......>>>>>....<<<<<<<
<..<<....<<<<<....>>>>>...>>...>>>>>.>>>...>>>>...
.>>>>>>>.

Receptor-Ligand Complex Structure

Structure summary

• PDB: 8uu7 Mechanistic insights into the alternative ribosome recycling by HflXr.
• Resolution: 3.2 Å
• Binding residue (original residue number in PDB): S11 Q14 K15 D44 R45 R48 A49 K54 G55 K56 S81 K110 P128 M137 D138 S141 L146 S147 R149 G150 S151 G152 E153 K154 K155 E157 R161 K164 V183 R184 R186 K187 V239 R240 Q376 V378 A379 N382 E383 K410
• Binding residue (residue number reindexed from 1): S9 Q12 K13 D42 R43 R46 A47 K52 G53 K54 S79 K108 P126 M135 D136 S139 L144 S145 R147 G148 S149 G150 E151 K152 K153 E155 R159 K162 V181 R182 R184 K185 V237 R238 Q374 V376 A377 N380 E381 K408

Gene Ontology

Molecular Function

• GO:0003924 GTPase activity
• GO:0005525 GTP binding
• GO:0043022 ribosome binding
• GO:0046872 metal ion binding

Cellular Component

• GO:0005737 cytoplasm

External links

• PDB: RCSB:8uu7, PDBe:8uu7, PDBj:8uu7
• PDBsum: 8uu7
• PubMed: 38407413
• UniProt: Q8Y8Y0