Structure of PDB 8cre Chain v Binding Site BS01

Receptor Information

>8cre Chain v (length=203) Species: 5476 (Candida albicans)

GAYKYLEELQRKKQSDVMRFLYRVRCWEYRQKNVIHRASRPSRPDKARRL
GYKAKQGFVIYRIRVRRGGRKRPVPKGATYGKPTNQGVNQLKYQKSLRST
AEERVGRRASNLRVLNSYWVNQDSTYKYFEVILVDPSHKAIRRDARYNWI
VNPVHKHREARGLTSAGKKSRGINKGHLFNKTKAGRRHTWKKHNTLSLWR
YRS

Ligand information

>8cre Chain 1 (length=3216)

ugaccucaaaucagguaggacuacccgcugaacuuaagcauaucaauaag
cggaggaaaagaaaccaacagggauugccucaguagcggcgagugaagcg
gcaaaagcucaaauuugaaaucuggcgucuuuggcguccgaguuguaauu
ugaagaagguaucuuugggcccggcucuugucuauguuccuuggaacagg
acgucacagagggugagaaucccgugcgaugagaugacccgggucugugu
aaaguuccuucgacgagucgaguuguuugggaaugcagcucuaagugggu
gguaaauuccaucuaaagcuaaauauuggcgagagaccgauagcgaacaa
guacagugauggaaagaugaaaagaacuuugaaaagagagugaaaaagua
cgugaaauuguugaaagggaagggcuugagaucagacuugguauuuugca
ugcgguuuaccgggccagcaucgguuuggagcggcaggauaauggcggag
gaauguggcacggcuucugcuguguguuauagccucugacgauacugcca
gccuagaccgaggacugcgguuuaccuaggauguuggcauaaugaucuua
agucgcccgucuugaaacacggaccaaggagucuaacgucuaugcgagug
uuuggguguaaaacccguacgcguaaugaaagugaacgaaggugggggcc
cauuagggugcaccaucgaccgauccugauguguucggauggauuugagu
aagagcauagcuguugggacccgaaagauggugaacuaugccugaauagg
gugaagccagaggaaacucugguggaggcucguagcgguucugacgugca
aaucgaucgucgaauuuggguauaggggcgaaagacuaaucgaaccaucu
aguagcugguuccugccgaaguuucccucaggauagcagaagcucguauc
aguuuuaugagguaaagcgaaugauuagaagucuugggguugaaaugacc
uuaacuuauucucaaacuuuaaauauguaagaaguccuuguugcuuaauu
gaacguggacaauugaaugaagagcuuuuagugggccauuuuugguaagc
agaacuggcgaugcgggaugaaccgaacgugaaguuaaagugccggaaug
cacgcucaucagacaccacaaaagguguuaguucaucuagacagccggac
gguggccauggaagucggaauccgcuaaggaguguguaacaacucaccgg
ccgaaugaacuagcccugaaaauggauggcgcucaagcgugcuacuuaua
cuucaccgugauugcuguuuugacgcuuucacgaguaggcaggcguggag
gucagugacgaagccuuugcuguaaagcugggucgaacggccucuagugc
agaucuuggugguaguagcaaauauucaaaugagaacuuugaagacugaa
guggggaaagguuccaugucaacagcaguuggacauggguuagucgaucc
uaagagauggggaagcuccguuucaacgugcuugauuuuucaggccaacc
aucgaaagggaauccgguuaaaauuccggaacuuggauauggauucuuca
cggcaacguaacugaauguggagacgucggcgugagcccugggaggaguu
aucuuuucuucuuaacagcuuaucacccuggaauugguuuauccggagau
ggggucuuauggcuggaagagcgcgguaauuuugccgcguccggugcgcu
uacgacgguccuugaaaauccacaggaaggaauaguuuucaugccaaguc
guacucauaaccgcagcaggucuccaagguuaacagccucuaguugauag
aauaauguagauaagggaagucggcaaaauagauccguaacuucgggaua
aggauuggcucuaaggaucgguuuaacgaucaacuuagaacugguacgga
caaggggaaucugacugucuaauuaaaacauagcauugugauggucagaa
agugauguugacacaaugugauuucugcccagugcucugaaugucaaagu
gaagaaauucaaccaagcgcggguaaacggcgggaguaacuaugacucuc
uuaagguagccaaaugccucgucaucuaauuagugacgcgcaugaaugga
uuaacgagauucccacugucccuaucuacuaucuagcgaaaccacagcca
agggaacgggcuuggcagaaucagcggggaaagaagacccuguugagcuu
gacucuaguuugacauugugaaaagacauggaggguguagaauaaguggg
agcuucggcgccggugaaauaccacuaccucuauaguuuuuuuacuuauu
caaugaagcggagcuggaggucaaacuccacguucuagcauuaagcccuc
ugggcgauccggguugaagacauugucagguggggaguuuggcuggggcg
gcacaucuguuaaacgauaacgcagguguccuaagggggacucauggaga
acagaaaucuccaguagaacaaaaggguaaaaguccccuugauuuugauu
uucagugugaauacaaaccaugaaaguguggccuaucgauccuuuagucc
cucggaauuugaggcuagaggugccagaaaaguuaccacagggauaacug
gcuuguggcagucaagcguucauagcgacauugcuuuuugauucuucgau
gucggcucuuccuaucauaccgaagcagaauucgguaagcguuggauugu
ucacccacuaauagggaacgugagcuggguuuagaccgucgugagacagg
uuaguuuuacccuacugaugaauguuaucgcaauaguaauugaacuuagu
acgagaggaaccguucauucagauaauugguuuuugcggcugucugauca
ggcaacgccgcgaagcuaccaucugcuggauuauggcugaacgccucuaa
gucagaauccaugcuagaacgcgaugauuuuugcccugcacauuuuagau
ggauacgaauaagacuuuuuagucgcuggaccauagcaggcuggcaacgg
ugcgcuuagcggaaaggcuuugugcgcuugccggcggauagcaaugucaa
caugcgcggggauaaauccuuugcauacgacuuagauguacaacggagua
uuguaagcaguagaguagccuuguuguuacgaucugcugagauuaagcuc
uuguugucugauuugu

.......................<<<<<<.....<......>......>>
>>>.>.........<<....>>..<<<<<......<<.....>>.....>
>>>>..[[[...........<<.<<<<<<...>>>>>>.>>.........
....<<<<<...<<<<<<<<<<<<.<<<<<<<<....<<<<......>>>
>.(.......<<<......)>>>..>.>>>>>>>....>>>>>>>>....
>>>>..>>>>>........<<<<<<<........>>>>>>>.....((((
(..<<<<<<)))))..]]]...............................
...<<....>>...............<<<<....>>>>............
......................<<<<<<<<<<<<.....<<<<..<<<<.
.>>>>..>>>>..<<<<<<<.<<<<<<<<.<<<<<<<....<<.<<<<<<
<......<<<<<<......>>>>>>.......>>>>>>.>.>>.>>>>>.
>>>>>>>>>>........<<<..>>>.....>>>>>>>....>>>>>>>>
>>>>..<<<<........>>>><<<<<<....<<<<<<.<<<<<<..<<<
..<<<<<.....>>>>>..>>>...<<....>>.......<<<<<..<<<
<....>>>>...>>>>>.<<<<<<<<<..<<....>>>..>>>.>>>.>>
....>>>>>>.>>>>>>..<<.<..<<<<<<<.....<<<<<<<<<..<<
<....<<<<<<<....>>>>>>>.....>>>...<<<<.<<.<<......
..>>>>>>>>....>>>>>>>>>...<<.......>>......>>>>>>.
..>..>.>>..<<<<<<....<.<<<....>>>.><<<<<<<<<<...<<
<.<<<<<<<.....<<<....<<<....<<<<...<<<<<<.....>>>>
>>.>>>>....>>>..>.>>.....>>>>>...<<<<.<<<<........
.>>>>.>>>>.....>>>>>.>>>>>>>>.>>..<<<<.<<<<<.....>
>>>>.>>>>....<<<......>>>...<<<<<<<..<<<<(((.....<
<<<<<.....<<..........<<<.<<<<<<<<<<.......<<<<<.<
<....<<.........>>....>>.........<<......>>...>>>>
>...>>>>>>>>>>>>>..........))).>>...>>>>>>.>>>>..>
>>>>>><<<<<..<<.........>>..>>>>>....>>>>>><.<<<<<
<<<.<<......<<<<..<<<....>>>.>>>>...>>>>>>>>>>..>.
....>>>>>><<.....<<.....<<<<<<.......>>>>>><<<<...
..<<<<<.<<...<<<<<<...<<.....>>.>>>>>>....((<<<<<<
...<<....<<<....>>>..........<<<<<<....>>>>>>.....
.>>....>>...<<<<<........>>>>>))<<<<.<<<<<<<.....<
<<....>>>.......<<<<<<...<<<<.<<<<<<<.<<<<<<<<<<..
{{{..>>>>>><...<<<<<....<<<<<<....<<<.....>>>....>
>>>>>.....>>>>>....><<<<<<<......>>>>>>>>>>>...>>>
>>>>.>>>>.........>>>>>>..........>>>..>>>>>>>>>>>
>..>>.....<<......>>>>>>>.<<<<.....>>>>..>>>>..<<<
<.....<<<<<<<.<<...............<<<<<..............
.>>>>><<.<<<<<<<<<<<......>>>>>..>>>>>>.>>........
....<<<<<<<<<..<<<.........>>>..<<<<<<<....<<<<...
..>>>>......>>>>>>>...<<..<<<..<<<<<<....<<<.....<
<<<....>>>>.....>>>.>>>>..>>>>><<<<<<.......>>>>>>
........<...........<<<<<.......>>>>>.....>.....>>
....>>.>>>>>>>.....>>.>>>>>>>..>>>>>>....>>....<<<
<<........>>>>>....[[[[[..{{{.{{......[[[[.{{[[[[.
.<<<<<.<<<<<<<<..<<<<<<<<<<...<.<<................
............................>>.>...>>>>>>>>>>...<<
<<<.....<<<<..<<<<<......>>>>>..............<<<<..
.>>>>..>>>>..>>>>>.....>>>>>>>>..>>>>>...<<<.....>
>>..<<<<<<...........>>>>>>..<<...<<<<<<<...<<<<<<
.......>>>>>>.....<<...(((>>....>>>>>>>...<<<.<<<<
<.......>>>>.>>>>.<<<....>>>.>>))).......((((((..(
(((.>>>>>>))))))))))..........]]]]..}}]]]].......<
<.<...<<<<<<...<<.......>>...>>>>>>....>.>.>......
.<<<<<.<<<<<<..<.<<<<<<<......>>>>>>>...>.<<<.....
....>>>.....>>>>>..>...>>>.>>....<<<<<<<....>>>.>>
>>....}}..}}}.]]]]]....<<<<<<<<..<<<<...<<<<<.....
............>>>>>..<<<<<...<<<<....<<<<<<<<<<}}}.>
>>>>..>>>>>...>>>>..>>>>>.<<<<...<<<<<...........>
>>>>...>>>>.>>>>....>>>>>>>>.....<<<<<<.<<<.<.....
<<..........<<<......>>>......>>.......<<<<<<...<<
<<<<<..<<<......>>>..>>>>>>>.>>>>>>..............>
.>>>.>>>>>>..<<<<<<<.............>>....<<<<<<<<<.<
<<....<<<<.<..<<<<<......>>>>>..>>>>>...>>>....>>>
>.>>>>>..>>>>>..

Receptor-Ligand Complex Structure

Structure summary

• PDB: 8cre Drug-induced rotational movement of the ribosome is a key factor for read-through enhancement
• Resolution: 3.0 Å
• Binding residue (original residue number in PDB): G2 Y4 K5 E8 R12 K14 Q15 R20 R24 R31 N34 V35 S40 R41 R44 K47 R49 R50 L51 K54 A55 K56 Q57 R67 R68 G69 G70 R71 K72 R73 P74 P76 K77 G78 Y81 G82 K83 P84 T85 N86 Q87 N90 Q91 K93 Y94 Q95 K96 S97 L98 R99 R105 R108 S111 N112 N117 W120 S125 Y127 K128 S138 H139 K140 A141 R144 R147 W150 P154 V155 H156 K157 H158 A161 R162 L164 S166 K170 S171 R172 I174 K176 G177 H178 L179 F180 N181 K182 R187 R188 H189 W191 K192 K193 H194 S198 W200 R201 Y202 R203
• Binding residue (residue number reindexed from 1): G1 Y3 K4 E7 R11 K13 Q14 R19 R23 R30 N33 V34 S39 R40 R43 K46 R48 R49 L50 K53 A54 K55 Q56 R66 R67 G68 G69 R70 K71 R72 P73 P75 K76 G77 Y80 G81 K82 P83 T84 N85 Q86 N89 Q90 K92 Y93 Q94 K95 S96 L97 R98 R104 R107 S110 N111 N116 W119 S124 Y126 K127 S137 H138 K139 A140 R143 R146 W149 P153 V154 H155 K156 H157 A160 R161 L163 S165 K169 S170 R171 I173 K175 G176 H177 L178 F179 N180 K181 R186 R187 H188 W190 K191 K192 H193 S197 W199 R200 Y201 R202

External links

• PDB: RCSB:8cre, PDBe:8cre, PDBj:8cre
• PDBsum: 8cre
• UniProt: Q5A6R1|RL15A_CANAL Large ribosomal subunit protein eL15 (Gene Name=RPL15A)