Structure of PDB 7oh3 Chain v Binding Site BS01

Receptor Information

>7oh3 Chain v (length=287) Species: 559292 (Saccharomyces cerevisiae S288C)

NARAKRALVKREAKLVENVKQALFIPGQSCNKNLHDIMVDLSALKKPDMK
RFNRKNDIHPFEDMSPLEFFSEKNDCSLMVLMTSSKKRKNNMTFIRTFGY
KIYDMIELMVADNFKLLSDFKKLTFTVGLKPMFTFQGAAFDTHPVYKQIK
SLFLDFFRGESTDLQDVAGLQHVISMTIQGDFQDGEPLPNVLFRVYKLKS
YKSDQGGKRLPRIELVEIGPRLDFKIGRIHTPSPDMVTEAHKKPKQKKNV
ELDIMGDKLGRIHMGKQDLGKLQTRKMKGLKSKFDQG

Ligand information

>7oh3 Chain 1 (length=3013)

guugaccucaaaucagguaggaguacccgcugaacuuaagcauaucaaua
agcggaggaaaagaaaccaaccgggauugccuuaguaacggcgagugaag
cggcaaaagcucaaauuugaaaucugguaccuucggugcccgaguuguaa
uuuggagagggcaacuuuggggccguuccuugucuauguuccuuggaaca
ggacgucauagagggugagaaucccguguggcgaggagugcgguucuuug
aaagugccuucgaagagucgaguuguuugggaaugcagcucuaagugggu
gguaaauuccaucuaaagcuaaauauuggcgagagaccgauagcgaacaa
guacagugauggaaagaugaaaagaacuuugaaaagagagugaaaaagua
cgugaaauuguugaaagggaagggcauuugaucagacaggccagcaucag
uuuugguggcaggauaaauccauaggaauguagcuugccugguaaguauu
auagccugugggaauacugccagcugggacugaggacugcgacguaaguc
aaggaugcuggcauaaugguuauaugccgcccgucuugaaacacggacca
aggagucuaacgucuaugcgaguguuuggguguaaaacccauacgcguaa
ugaaagugaacguagguuggggccucgcaagaggugcacaaucgaccgau
ccugaugucuucggauggauuugaguaagagcauagcuguugggacccga
aagauggugaacuaugccugaauagggugaagccagaggaaacucuggug
gaggcucguagcgguucugacgugcaaaucgaucgucgaauuuggguaua
ggggcgaaagacuaaucgaaccaucuaguagcugguuccugccgaaguuu
cccucaggauagcagaagcucguaucaguuuuaugagguaaagcgaaaac
uuuaaauauguaagaaguccuuguuugaacguggacaugaaugaagagcu
uuuagugggccauuuuugguaagcagaacuggcgaugcgggaugaaccga
acguagaguuaaggugccggaauacacgcucaucagacacacaaaaggug
uuaguucaucuagacagccggacgguggccauggaagucggaauccgcua
aggaguguguaacaacucaccggccgaaugaacuagcccugaaaauggau
ggcgcucaagcguguuaccuauacucuaccgucaggguugugaugcccug
acgaguaggcaggcguggaggucagugacgaagccuagaccguaaggucg
ggucgaacggccucuagugcagaucuuggugguaguagcaaauauucaaa
ugagaacuuugaagacugaaguggggaaagguuccacgucaacagcaguu
ggacguggguuagucgauccuaagagauggggaagcuccguuucaaaggc
cuaggccaccaucgaaagggaauccgguuaagauuccggaaccuggauau
ggauucuucacgguaacguaacugaauguggagacgucggcgcgagcccu
gggaggaguuaucuuuucuucuuaacagcuuaucaccccggaauugguuu
auccggagauggggucuuauggcuggaagaggccagcacugcuggcuccg
gugcgcuugugacggcccgugaaaauccacaggaaggaauaguuuucaug
ccaggucguacugauaaccgcagcaggucuccaaggugaacagccucuag
uugauagaauaauguagauaagggaagucggcaaaauagauccguaacuu
cgggauaaggauuggcucuaagggucggacgaucaacuuagaacugguac
ggacaaggggaaucugacugucuaauuaaaacauagcauugcgaugguca
gaaagugauguugacgcaaugugauuucgcccagugcucugaaugucaaa
gugaagaaauucaaccaagcgcggguaaacggcgggaguaugacucucuu
aagguagccaaauggauuaacgagauucccacugucccuaucuacuaucu
agcgaaaccacagccaagggggcuuggcagaaucagcggggaagaagacc
cugugagcuugacucuaguuugacauugugaagagacauaguuucuuuac
uuauucaaugaagcggagcuggaauuauuuuccacguucuagcauucaag
gucccucggggcugauccgguugaagacauugucagguggggaguuuggg
ggcggcacaucuguuaaacgauaagcagauguccuaaggggggcucaugg
agaacagaaaucuccaguagaacaaaaggguaaaagcccccuugauuuug
auuuucagugugaauacaaaccaugaaaguguggccuaucgaucuuuagu
cccucggaauuugaggcuagaggugccagaaaguuaccacagggauaacu
ggcuuguggcagucaagcguucagcgacauugcuuuugauucuucgaugu
cggcucuuccuaucauaccgaagcagaauucgguaagcguuggauuguuc
acccacuaauagggaguagcuggguuuagaccgucgugagacagguuagu
uuacccuacugaugaauguuaccgcaauaguaauugaacuuaguacgaga
ggaacaguucauucggauaauugguuuuugcggcugucugaucaggcauu
gccgcgaagcuaccauccgcuggauuauggcugaacgccucuaagucaga
auccaugcuagaacgcggugauuucugcuccacacaauauagauggauac
gaauaaggcguccuuguggcgucgcugaaccauagcaggcuagcaacggu
gcacuuggcggaaaggccuugggugcuugcuggcgaauugcaaugucauu
uugcguggggauaaaucauuuguauacgacuuagauguacaacgggguau
uguaagcaguagaguagccuugguuacgaucugcugagauuaagccuuug
uugucugauuugu

.........................<<<<<<.....<<....>>......
>>>>>.>.........<<....>>..<<<<<......<<.....>>....
.>>>>>..<<<..<<.......<<.<<<<<<...>>>>>>.>>.......
>>....<<<<<<.<<<<<<<<<<<<<<<<<<<<<<....<<<<......>
>>>.((......<<<.....))>>>..>.>>>>>>>>..>>>>>>>>...
>>>>>>>>>>>........<<<<<<<........>>>>>>>.....<<<<
<..((((((>>>>>..>>>...............................
...<<....>>...............<<<<....>>>>............
......................<<<<<.<<<<<......<<<<<<<.<<<
<<<<<<<<<<<<.....<<<<<<<<<......<<<<<<...>>>>>>...
....>>>>>>>.>.>.>>>>>.>>>>>>>>>>........<<<....>>>
.....>>>>>>>.....>>>>>.>>>>>..<<<<........>>>><<<<
<<....<<<<<<.<<<<<<..<<<...<<<<.....>>>>...>>>...<
<....>>.......<<<<<..<<<<<....>>>>>...>>>>>.<<<<<<
<<...<......>...>>>.>>>.>>....>>>>>>.>>>>>>..<<.<.
..<<<<<<.....<<<<<<<<<..<<<....<<<<<<<....>>>>>>>.
....>>>...<<<<.<<.<<........>>>>>>>>....>>>>>>>>>.
..<<.......>>......>>>>>>......>.>>..<<<<<<....<.<
<<....>>>.><<.<<<<......<<<.<<<<<<....<..<........
.>..>....>>>>...<<<<.<<<....>>>.>>>>...>>>>>....>>
>>..>>..<<<<.<<<<<.....>>>>>.>>>>....<<<......>>>.
..<<<<<<<..<<<<(((.....<<<<<<.....<<.........<<<.<
<<<<<<<<<.......<<<<<.<<....<<.........>>....>>...
......<<......>>...>>>>>...>>>>>>>>>>>>>..........
))).>>...>>>>>>.>>>>..>>>>>>><<<<<<<<<......>>>>>>
>>>....>>>>>><.<<<<<<<<.<<......<<<<.<<<<....>>>>>
>>>...>>>>>>>>>>..>.....>>>>>><<.....<<.....<<<<<<
.......>>>>>><<<<.....<<<<<.<<...<<<<<<...<<.....>
>.>>>>>>....((<<<<<<...<<....<<<....>>>........<<<
<..>>>>....>>....>>...<<<<<........>>>>>))<<<<.<<<
<<<<.....<<<....>>>...<.{{<<<<<<...<<<<.<<<<<<<.<<
<<<<<<<<..[[[..>>>>>><...<<<<<....<<<<<<....<<<...
..>>>....>>>>>>.....>>>>>....><<<<<<<...>>>>>>>>>>
>...>>>>>>>.>>>>.........>>>>>>...>......>>>..>>>>
>>>>>>>>..>>.....<<......>>>>>>>.<<<<.....>>>>..>>
>>..<<<<.....<<<<<<<.<<.........<.....<<<<<.......
........>>>>><<.<<<<<<<<<<<..>>>>>..>>>>>>.>>.....
.......<<<<<<<<<..<<<.........>>>..<<<<<<<....<<<<
.....>>>>......>>>>>>>.............<<<.<<..<<<....
.<<<<....>>>>.....>>>>>>>>........<<.<......>.>>..
........<<..>>......>>.>>>>>>>..>..>>.>>>>>>>..>>>
>>>....>>....<<<<<....>>>>>....<<<<<..<<<<......<<
<<<<<<<<<..<<<<<.<<<<<<<<..<<<<<<<<<<...>>>>>>>>>>
...<<<<<.....<<<<...<<<<.....>>>>...........}}...<
<<<<<..>>>>>>..>>>>.>>>>>.....>>>>>>>>..>>>>>.....
.<..<...<<<<<<..........>>>>>>.......<<<<<<<...<<<
<<<.......>>>>>>.....<<......>>....>>>>>>>...<<<..
<<<<.......>>>>..>>>.<<<....>>>...>.>......<<<<<..
.<<<<.))))))>>>>.>>>>>.........>>>>..>>>>>>>......
.......<<<<<<...<<.....>>...>>>>>>................
<<<<...<<<<..<.<<<<<<<......>>>>>>>...>.<<<.......
..>>>.....>>>>....>>>>......<<<<<<<....>>>.>>>>...
.>..>>>.>>>>>....<<<<<<<<..<<<<.<.<<<<<<..........
.....>>>>>>.><<<<<...<<<<...<<<<<<<<<<<]]].>>>>>..
>>>>>>..>>>>..>>>>>.<<<<...<<<<<...........>>>>>..
.>>>>.>>>>....>>>>>>>>.....<<<<<<.<<<.<<....<<....
......<<<<<<.....>>>>>>......>>.......<<<<<<...<<<
<<.<..<<<......>>>..>.>>>>>.>>>>>>.............>>.
>>>.>>>>>>..<<<<<.<..<.......>..>.....<<<<<<<<<.<<
<....<<<<....<<<<<....>>>>>...>>>>...>>>....>>>>.>
>>>>..>>>>>..

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7oh3 Analysis of subunit folding contribution of three yeast large ribosomal subunit proteins required for stabilisation and processing of intermediate nuclear rRNA precursors.
• Resolution: 3.4 Å
• Binding residue (original residue number in PDB): N9 R11 A12 R14 L16 K18 N61 R62 F77 K81 N82 Q254 K261 N262 H276 M277 K279 Q280 L282 Q286 T287 R288 K289 L293
• Binding residue (residue number reindexed from 1): N1 R3 A4 R6 L8 K10 N53 R54 F69 K73 N74 Q246 K248 N249 H263 M264 K266 Q267 L269 Q273 T274 R275 K276 L280

Gene Ontology

Molecular Function

• GO:0005515 protein binding
• GO:0008097 5S rRNA binding
• GO:0008312 7S RNA binding
• GO:0019843 rRNA binding

Biological Process

• GO:0000027 ribosomal large subunit assembly
• GO:0000463 maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
• GO:0000466 maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
• GO:0000470 maturation of LSU-rRNA
• GO:0006364 rRNA processing
• GO:1902626 assembly of large subunit precursor of preribosome

Cellular Component

• GO:0005634 nucleus
• GO:0005730 nucleolus
• GO:0030687 preribosome, large subunit precursor

External links

• PDB: RCSB:7oh3, PDBe:7oh3, PDBj:7oh3
• PDBsum: 7oh3
• PubMed: 34813592
• UniProt: P36160|RPF2_YEAST Ribosome biogenesis protein RPF2 (Gene Name=RPF2)