Structure of PDB 7z20 Chain u Binding Site BS01

Receptor Information

>7z20 Chain u (length=102) Species: 562 (Escherichia coli)

AAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKKHQKPVPA
LNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSE
TI

Ligand information

>7z20 Chain b (length=2901)

gguuaagcgacuaagcguacacgguggaugcccuggcagucagaggcgau
gaaggacgugcuaaucugcgauaagcgucgguaaggugauaugaaccguu
auaaccggcgauuuccgaauggggaaacccaguguguuucgacacacuau
cauuaacugaauccauagguuaaugaggcgaaccgggggaacugaaacau
cuaaguaccccgaggaaaagaaaucaaccgagauucccccaguagcggcg
agcgaacggggagcagcccagagccugaaucaguguguguguuaguggaa
gcgucuggaaaggcgcgcgauacagggugacagccccguacacaaaaaug
cacaugcugugagcucgaugaguagggcgggacacgugguauccugucug
aauauggggggaccauccuccaaggcuaaauacuccugacugaccgauag
ugaaccaguaccgugagggaaaggcgaaaagaaccccggcgaggggagug
aaaaagaaccugaaaccguguacguacaagcagugggagcacgcuuggcg
ugugacugcguaccuuuuguauaaugggucagcgacuuauauucuguagc
aagguuaaccgaauaggggagccgaagggaaaccgagucuuaacugggcg
uuaaguugcaggguauagacccgaaacccggugaucuagccaugggcagg
uugaagguuggguaacacuaacuggaggaccgaaccgacuaauguugaaa
aauuagcggaugacuuguggcugggggugaaaggccaaucaaaccgggag
auagcugguucuccccgaaagcuauuuagguagcgccucgugaauucauc
uccggggguagagcacuguuucggcaagggggucaucccgacuuaccaac
ccgaugcaaacugcgaauaccggagaauguuaucacgggagacacacggc
gggugcuaacguccgucgugaagagggaaacaacccagaccgccagcuaa
ggucccaaagucaugguuaagugggaaacgaugugggaaggcccagacag
ccaggauguuggcuuagaagcagccaucauuuaaagaaagcguaauagcu
cacuggucgagucggccugcgcggaagauguaacggggcuaaaccaugca
ccgaagcugcggcagcgacgcuuaugcguuguuggguaggggagcguucu
guaagccugcgaaggugugcugugaggcaugcuggagguaucagaagugc
gaaugcugacauaaguaacgauaaagcgggugaaaagcccgcucgccgga
agaccaaggguuccuguccaacguuaaucggggcagggugagucgacccc
uaaggcgaggccgaaaggcguagucgaugggaaacagguuaauauuccug
uacuugguguuacugcgaaggggggacggagaaggcuauguuggccgggc
gacgguugucccgguuuaagcguguaggcugguuuuccaggcaaauccgg
aaaaucaaggcugaggcgugaugacgaggcacuacggugcugaagcaaca
aaugcccugcuuccaggaaaagccucuaagcaucagguaacaucaaaucg
uaccccaaaccgacacagguggucagguagagaauaccaaggcgcuugag
agaacucgggugaaggaacuaggcaaaauggugccguaacuucgggagaa
ggcacgcugauauguaggugaagcgacuugcucguggagcugaaaucagu
cgaagauaccagcuggcugcaacuguuuauuaaaaacacagcacugugca
aacacgaaaguggacguauacggugugacgccugcccggugccggaaggu
uaauugaugggguuagcgcaagcgaagcucuugaucgaagccccgguaaa
cggcggccguaacuauaacgguccuaagguagcgaaauuccuugucgggu
aaguuccgaccugcacgaauggcguaaugauggccaggcugucuccaccc
gagacucagugaaauugaacucgcugugaagaugcaguguacccgcggca
agacggaaagaccccgugaaccuuuacuauagcuugacacugaacauuga
gccuugauguguaggauaggugggaggcuuagaaguguggacgccagucu
gcauggagccgaccuugaaauaccacccuuuaauguuugauguucuaacg
uugacccguaauccggguugcggacagugucugguggguaguuugacugg
ggcggucuccuccuaaagaguaacggaggagcacgaagguuggcuaaucc
uggucggacaucaggagguuagugcaauggcauaagccagcuugacugcg
agcgugacggcgcgagcaggugcgaaagcaggucauagugauccgguggu
ucugaauggaagggccaucgcucaacggauaaaagguacuccggggauaa
caggcugauaccgcccaagaguucauaucgacggcgguguuuggcaccuc
gaugucggcucaucacauccuggggcugaaguaggucccaagggugaugc
uguucgccauuuaaagugguacgcgagcuggguuuagaacgucgugagac
aguucggucccuaucugccgugggcgcuggagaacugaggggggcugcuc
cuaguacgagaggaccggaguggacgcaucacugguguucggguugucau
gccaauggcacugcccgguagcuaaaugcggaagagauaagugcugaaag
caucuaagcacgaaacuugccccgagaugaguucucccugacccuuuaag
gguccugaaggaacguugaagacgacgacguugauaggccggguguguaa
gcgcagcgaugcguugagcuaaccgguacuaaugaaccgugaggcuuaac
c

<<<<<<<<......<<<<<<<<<<<.....[[..[[<<<<<<<[[<<...
...<<<<<<.[..(((.>>>.....<<<<<<<..<<<)))..].>>>...
...>>>>>>>...>>>....<<<....>>><<<<<<<....>>>>>>>.<
<<<<<<<<.........>>>>>>>>>.>>...<<<<<<.....<<....>
>......>>>>>.>.........<<....>>..<<<<<<.....<<....
.>>....>>>>>>..(((..<<<<......<<<<<<<<<<<<<.<<<...
<<<<<......>>>>>((......<<<.....))>>>...>>>...>>>>
>>>>>>>>>...>>>>........<<<<<<<<.........>>>>>>>>.
.....<<<<<..<<<<>>>>>..)))......]].>>>>>>>.]].....
..........<<....>>....]].........<<<<.....>>>>....
..............>>>>>>>>>>>.......<<<<..<<<<<<<.>>>>
>>>..>>>>...<.<........>.>.<<<<<<....<<<<<<<<<<<<<
..<<<...<<.....>>...>>>...<<....>>..<<<<.....>>>>.
....>>>>>>>>>>>>>..<<.<...<<<<<<.....<<<<<<<<<..<<
<....<<<<<<......>>>>>>.....>>>...<<<.<<<<<.......
.>>>>>>>>....>>>>>>>>>...<<<.....>>>......>>>>>>..
....>.>>..<<<<<<....<<<<<....>>>>>.<<<<<<<<...<<<<
<<<<<.....<<<...<<<.<<<<...<<................>>...
>>>>.>>>..>>.>.....>>>>>>.>>>...>>>>>>>.>...<<<<<<
<<<<......>>>>>>>>>>....<<<......>>>...<<<<.<<<...
<<<(((.....<<<<<<...........<<..<<<<<..<<........<
<.........<.............>...............<......>..
.....>>....>>..>>>>>.>>...........))).....>>>>>>.>
>>...>>>>>>><<<<<<<........>>>>>>>....>>>>>>..<<<<
<...<<<......<<<<<<<<....>>>>>>>>...>>>..>>>>>....
....>>>>>><......<<.....<<<<<<.......>>>>>><<<<...
.<<<<<.<<...<<<<<<...<<.....>>.>>>>>>....((<<<.<<<
...<<<...<<<....>>>...>>>...>>>..<<<<<........>>>>
>))<<<<<<<<<<<<<...<<<<....<<<<..<<<..<<<<<<<<<<<.
...(((...>>>>>><<..<<<<...<<<<<<<<<<<<.<<.....>>>>
>>>>>>..>>>>...>>>>...>><..<<<<<....>>>>>...>>>>>>
...>>>...>>>>.........>>>>...>>....>>>>>>>>>>>.>>>
..>>.....<<......>>>>>>>.<<<<.....>>>>..>>>>..<<<<
....<<<<<<<<.<<.........<.....<<<<<...............
>>>>><<<<<<.....<<<...<<<<.....>>>>...>>>...>>>>>>
............<<<<<<<<<..<<<.........>>>..<<<<<<<...
..<<<....>>>.......>>>>>>>.<<<<<.<<<..<<<<<<<..<<<
<...<...<<<<<<.<<....>>..>>>>>>..>....>>>>>>>>>..>
>>>><<<<<<.......>>>>>>........<...........<<<<<<.
....>>>>>>.....>....>>>>>..>>.>>>>>>>..>..>>.>>>>>
>>>.>>>>>>.....>.....<<<<<........>>>>>..<<<<<<<<.
(((.[[......<<<<.[[<<<<..<<<<<.<<<.<<<<<.<<<<<<<<<
<<................................................
..................................>>>>>>>>>>>...<<
<.<<<<<<.....>>>>>>>>>....>>>>>.>>>..>>>>>..<<<<..
...>>>><<<<<<...........>>>>>>..<<...<<<<<<<...<<<
<<<.......>>>>>>.....<<...(((>>....>>>>>>>...<<<<.
.<<<<....>>>>..>>>>.<<<....>>>.>>))).......<<<<<<<
.((..>>>>..))>>>>>>>.............>>>>..]]>>>>.....
..<<<<.<.<<<<<<...<<.......>>...>>>>>>...>>>>.>...
....<<<<<.<<<<<<..<<<<<<<<<......>>>>>>..>>>.<..<<
.....>>..>.....>>>>>..>...>>>.>>....<<<<<<<....>>>
.>>>>....]]..)))>>>>>>>>....<<<<<<<...<<<<..<<<<<<
<...............>>>>>>>.<<<<<...<<<<...<<<<<<<<<<<
))).>>>>>>..>>>>>...>>>>.>>>>>...<<<....<<<<<.....
......>>>>>....>>>.>>>>.......>>>>>>>..<<<<<.....>
>>>><....<<..<<<<<.......>>>>>....<<<<<<<<..<<....
<<<<<....>>>>>...>>...>>>>>.>>>.....>>..>..>>>>>>>
>

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7z20 Modulating co-translational protein folding by rational design and ribosome engineering.
• Resolution: 2.29 Å
• Binding residue (original residue number in PDB): K4 R6 R7 G16 V28 K43 K44 H45 P48 G57 I58 Q66 S68 N69 R82 F85 K91 K92
• Binding residue (residue number reindexed from 1): K3 R5 R6 G15 V27 K42 K43 H44 P47 G56 I57 Q65 S67 N68 R81 F84 K90 K91

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003735 structural constituent of ribosome
• GO:0005515 protein binding
• GO:0019843 rRNA binding
• GO:0070180 large ribosomal subunit rRNA binding

Biological Process

• GO:0000027 ribosomal large subunit assembly
• GO:0002181 cytoplasmic translation
• GO:0006412 translation

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:7z20, PDBe:7z20, PDBj:7z20
• PDBsum: 7z20
• PubMed: 35869078
• UniProt: P60624|RL24_ECOLI Large ribosomal subunit protein uL24 (Gene Name=rplX)