Structure of PDB 6vu3 Chain u Binding Site BS01

Receptor Information

>6vu3 Chain u (length=144) Species: 562 (Escherichia coli)

MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGGGVRRGF
EGGQMPLYRRLPKFGFTSRKAAITAEIRLSDLAKVEGGVVDLNTLKAANI
IGIQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE

Ligand information

>6vu3 Chain a (length=2880)

gguuaagcgacuaagcguacacgguggaugcccuggcagucagaggcgau
gaaggacgugcuaaucugcgauaagcgucgguaaggugauaugaaccguu
auaaccggcgauuuccgaauggggaaacccaguguguuucgacacacuau
cauuaacugaauccauagguuaaugaggcgaaccgggggaacugaaacau
cuaaguaccccgaggaaaagaaaucaaccgagauucccccaguagcggcg
agcgaacggggagcagcccagagccugaaucaguguguguguuaguggaa
gcgucuggaaaggcgcgcgauacagggugacagccccguacacaaaaaug
cacaugcugugagcucgaugaguagggcgggacacgugguauccugucug
aauauggggggaccauccuccaaggcuaaauacuccugacugaccgauag
ugaaccaguaccgugagggaaaggcgaaaagaaccccggcgaggggagug
aaaaagaaccugaaaccguguacguacaagcagugggagcacgcuuaggc
gugugacugcguaccuuuuguauaaugggucagcgacuuauauucuguag
caagguuaaccgaauaggggagccgaagggaaaccgagucuuaacugggc
guuaaguugcaggguauagacccgaaacccggugaucuagccaugggcag
guugaagguuggguaacacuaacuggaggaccgaaccgacuaaugaaaaa
uuagcggaugacuuguggcugggggugaaaggccaaucaaaccgggagau
agcugguucuccccgaaagcuauuuagguagcgccucgugaauucaucuc
cggggguagagcacuguuucggcaagggggucaacccgacuuaccaaccc
gaugcaaacugcgaauaccggagaauguuaucacgggagacacacggcgg
ggcuaacguccgucgugaagagggaaacaacccagaccgccagcuaaggu
cccaaagucaugguuaagugggaaacgaugugggaaggcccagacagcca
ggauguuggcuuagaagcagccaucauuuaaagaaagcguaauagcucac
uggucgagucggccugcgcggaagauguaacggggcuaaaccaugcaccg
aagcugcggcagcgacgcuuaugcguuguuggguaggggagcguucugua
agccugcgaaggugugcugugaggcaugcuggagguaucagaagugcgaa
ugcugacauaaguaacgauaaagcgggugaaaagcccgcucgccggaaga
ccaaggguuccuguccaacguuaaucggggcagggugagucgaccccuaa
ggcgaggccgaaaggcguagucgaugggaaacagguuaauauuccuguac
uugguguuacugcgaaggggggacggagaaggcuauguuggccgggcgac
gguugucccgguuuaagcguguaggcugguuuuccaggcaaauccggaaa
aucaaggcugaggcgugaugacgaggcacuacggugcugaagcaacaaau
gcccugcuuccaggaaaagccucuaagcaucagguaacaucaaaucguac
cccaaaccgacacgguggucagguagagaauaccaaggcgcuugagagaa
cucgggugaaggaacuaggcaaaauggugccguaacuucgggagaaggca
cgcugauauguaggugaggucccucgcggauggagcugaaaucagucgaa
gauaccagcuggcugcaacuguuuauuaaaaacacagcacugugcaaaca
cgaaaguggacguauacggugugacgccucccggugccggaagguuaauu
gaugggguuagcgcaagcgaagcucuugaucgaagccccgguaaacggcg
gccgaacaaacgguccuaagguagcgaaauccuugucggguaaguuccga
cugcacgaauggcguaaugauggccaggcugucuccacccgagacucagu
gaaauugaacucgcugugagaugcaguguacccgcggcaagacggaaaga
ccccguaaccuuacuauagcuugacacugaacauugagccuugaugugua
ggauaggugggaggcuuugaaguguggacgccagucugcauggagccgac
cuugaaauaccacccuuuaauguuugauguucuaacguugacccguaauc
cggguugcggacagugucugguggguaguuugacugggcggucuccuccu
aaagaguaacggaggagcacgaagguuggcuaauccuggucggacaucag
gagguuagugcaauggcauaagccagcuugacugcgagcgugacggcgcg
agcaggugcgaaagcaggucauagugauccggugguucugaauggaaggg
ccaucgcucaacggauaaaagguacuccgggauaacaggcgauaccgccc
aagaguucauaucgacggcgguguuuggcacucggucggcucaucacauc
cuggggcugaaguaggucccaaggguauggcguucgccauuuaaaguggu
acgcgagcggguuuagaacgucgugagacagucggucccuaucugccgug
ggcgcuggagaacugaggggggcugcuccuaguacgagaggaccggagug
gacgcaucacugguguucggguugucaugccaauggcacugcccgguagc
uaaaugcggaagagauaagugcugaaagcaucuaagcacgaaacuugccc
cgagaugaguucucccugacccuuuaaggguccugaaggaacguugaaga
cgacgacguugauaggccggguguguaagcgcagcgaugcguugagcuaa
ccgguacuaaugaaccgugaggcuuaaccu

<<<<<<<<......<<<<<<<<<<<.....<<..<<<<<<<<<<<<<...
...<<<<<<.[..(((.>>>.....<<<<<<<..<<<)))..].>>>...
...>>>>>>>...>>>....<<<....>>><<<<<<<....>>>>>>>.<
<<<<<<<<.........>>>>>>>>>.>>...<<<<<<.....<<....>
>......>>>>>.>.........<<....>>..<<<<<<.....<<....
.>>....>>>>>>..<<<..<<<<.<....<<<<<<<<<<<<<.<<<...
<<<<<......>>>>>((......<<<.....))>>>...>>>...>>>>
>>>>>>>>>..>>>>>........<<<<<<<<.........>>>>>>>>.
.....<<<<<..((((>>>>>..>>>......>>.>>>>>>>.>>.....
..........<<....>>....>>.........<<<<.....>>>>....
..............>>>>>>>>>>>.......<<<<..<<<<<<....>>
>>>>..>>>>...<............>.<<<<<<....<<<<<<<<<<<<
<..<<<...<<.....>>...>>>...<<....>>..<<<<.....>>>>
.....>>>>>>>>>>>>>..<<.<...<<<<<<.....<<<<<<<<<..<
<<....<<<<<<......>>>>>>.....>>>...<<<.<<<<<.....>
>>>>>>>....>>>>>>>>>...<<<.....>>>......>>>>>>....
..>.>>..<<<<<<....<<<<<....>>>>>.<<<<<<<<...<<<<<<
<<<.....<<<...<<<.<<<<...<<.<<<<.....>>>>..>>...>>
>>.>>>..>>.>.....>>>>>>>.>>...>>>>>>>.>...<<<<<<<<
<.......>>>>>>>>>....<<<......>>>...<<<<.<<<...<<<
(((.....<<<<<<...........<<..<<<<<..<<........<<<<
<.<<...<<<<.......>>>>...>>........<<<......>>>..>
>>>>....>>..>>>>>.>>...........))).....>>>>>>.>>>.
..>>>>>>><<<<<<<<<....>>>>>>>>>....>>>>>>..<<<<<..
.<<<......<<<<<<<<....>>>>>>>>...>>>..>>>>>.......
.>>>>>><......<<.....<<<<<<.......>>>>>><<<<....<<
<<<.<<...<<<<<<...<<.....>>.>>>>>>....((<<<.<<<...
<<<...<<<....>>>...>>>...>>>..<<<<<........>>>>>))
<<<<<<<<<<<<<...<<<<....<<<<..<<<..<<<<<<<<<<<....
[[[...>>>>>><<..<<<<...<<<<<<<<<<<<.<<.....>>>>>>>
>>>..>>>>...>>>>...>><..<<<<<....>>>>>...>>>>>>...
>>>...>>>>.........>>>>...>>....>>>>>>>>>>>.>>>..>
>.....<<.....>>>>>>>.<<<<.....>>>>..>>>>..<<<<....
<<<<<<<<.<<.........<.....<<<<<...............>>>>
><<<<<<.....<<<...<<<<.....>>>>...>>>...>>>>>>....
........<<<<<<<<<..<<<.........>>>..<<<<<<<.....<<
<....>>>.......>>>>>>>.<<<<<.<<..<<<<<<<..<<<....<
...<<<<<..<<....>>...>>>>>.>......>>>>>>>>..>>>>..
<<<<......>>>>.........<..........<<<<<<.....>>>>>
>....>....>>>>>..>>.>>>>>>>..>..>>.>>>>>>>>.>>>>>>
.....>.....<<<<<.......>>>>>..<<<<<<<<.<<<.<<.....
.<.<<..<<<..<<<<<.<<<.<<<<<.<<<<<<<<<<<.<<<<......
.....<<<<<..<<.......<<<<<<<<....>>>>>>>>....>>...
.........>>>>>..>>>>.>>>>>>>>>>>...<<<.<<<<<<.....
>>>>>>>>>....>>>>>.>>>..>>>>>..<<<<....>>>><<<<<<.
..........>>>>>>..<<...<<<<<<<...<<<<<<.......>>>>
>>.....<<...(((>>....>>>>>>>...<<<<..<<<<....>>>>.
.>>>>.<<<....>>>.>>))).......<<<<<<<.<<..))))..>>>
>>>>>>..............>>>....>>>........<<...<<<<<<.
..<<.......>>...>>>>>>.....>>......<<.<<.<<<<<<..<
<<<<<<<<......>>>>>>>..>>.<<<<<....>>>>>.....>>>>>
..>...>>.>>....<.<<<<<....>>>.>>>....>>..>>>>>>>>>
>>....<<<<<<<...<<<<..<<<<<<<...............>>>>>>
>.<<<<<...<<<<...<<<<<<<<<<<]]].>>>>>>..>>>>>...>>
>>.>>>>>...<<<....<<<<<...........>>>>>....>>>.>>>
>.......>>>>>>>..<<<<<.....>>>>><....<<..<<<<<....
...>>>>>....<<<<<<<<..<<....<<<<<....>>>>>...>>...
>>>>>.>>>.....>>..>..>>>>>>>>.

Receptor-Ligand Complex Structure

Structure summary

• PDB: 6vu3 Structural basis of transcription-translation coupling.
• Resolution: 3.7 Å
• Binding residue (original residue number in PDB): N4 G11 S12 K13 K14 K17 R18 L19 G20 R21 G22 G24 S25 G28 K29 T30 G32 R33 G34 H35 K36 G37 Q38 K39 S40 R41 S42 G43 R47 R48 F50 E51 G52 G53 Q54 M55 Y58 R59 R60 P62 K63 F64 G65 F66 T67 S68 R69 K70 A71 E76 R78 D81 N99 I103 Q104 K109 I111 L112 R126 T128 K129 G130
• Binding residue (residue number reindexed from 1): N4 G11 S12 K13 K14 K17 R18 L19 G20 R21 G22 G24 S25 G28 K29 T30 G32 R33 G34 H35 K36 G37 Q38 K39 S40 R41 S42 G43 R47 R48 F50 E51 G52 G53 Q54 M55 Y58 R59 R60 P62 K63 F64 G65 F66 T67 S68 R69 K70 A71 E76 R78 D81 N99 I103 Q104 K109 I111 L112 R126 T128 K129 G130

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome
• GO:0005515 protein binding
• GO:0019843 rRNA binding

Biological Process

• GO:0002181 cytoplasmic translation
• GO:0006412 translation

Cellular Component

• GO:0005737 cytoplasm
• GO:0005840 ribosome
• GO:0015934 large ribosomal subunit
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:6vu3, PDBe:6vu3, PDBj:6vu3
• PDBsum: 6vu3
• PubMed: 32820061
• UniProt: P02413|RL15_ECOLI Large ribosomal subunit protein uL15 (Gene Name=rplO)