Structure of PDB 8p18 Chain t Binding Site BS01

Receptor Information

>8p18 Chain t (length=88) Species: 562 (Escherichia coli)

SLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFAEHKKDHH
SRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR

Ligand information

>8p18 Chain 2 (length=1536)

aaauugaagaguuugaucauggcucagauugaacgcuggcggcaggccua
acacaugcaagucgaacgguaacaggaagaauucuuugcugacgaguggc
ggacgggugaguaaugucugggaaacugccugauggagggggauaacuac
uggaaacgguagcuaauaccgcauaacgucgcaagaccaaagagggggac
cuucgggccucuugccaucggaugugcccagaugggauuagcuaguaggu
gggguaacggcucaccuaggcgacgaucccuagcuggucugagaggauga
ccagccacacuggaacugagacacgguccagacuccuacgggaggcagca
guggggaauauugcacaaugggcgcaagccugaugcagccaugccgcgug
uaugaagaaggccuucggguuguaaaguacuuucagcggggaggaaggga
guaaaguuaauaccuuugcucauugacguuacccgcagaagaagcaccgg
cuaacuccgugccagcagccgcgguaauacggagggugcaagcguuaauc
ggaauuacugggcguaaagcgcacgcaggcgguuuguuaagucagaugug
aaauccccgggcucaaccugggaacugcaucugauacuggcaagcuugag
ucucguagagggggguagaauuccagguguagcggugaaaugcguagaga
ucuggaggaauaccgguggcgaaggcggcccccuggacgaagacugacgc
ucaggugcgaaagcguggggagcaaacaggauuagauacccugguagucc
acgccguaaacgaugucgacuuggagguugugcccuugaggcguggcuuc
cggagcuaacgcguuaagucgaccgccuggggaguacggccgcaagguua
aaacucaaaugaauugacgggggcccgcacaagcgguggagcaugugguu
uaauucgaugcaacgcgaagaaccuuaccuggucuugacauccacggaag
uuuucagagaugagaaugugccuucgggaaccgugagacaggugcugcau
ggcugucgucagcucguguugugaaauguuggguuaagucccgcaacgag
cgcaacccuuauccuuuguugccagcgguccggccgggaacucaaaggag
acugccagugauaaacuggaggaagguggggaugacgucaagucaucaug
gcccuuacgaccagggcuacacacgugcuacaauggcgcauacaaagaga
agcgaccucgcgagagcaagcggaccucauaaagugcgucguaguccgga
uuggagucugcaacucgacuccaugaagucggaaucgcuaguaaucgugg
aucagaaugccacggugaauacguucccgggccuuguacacaccgcccgu
cacaccaugggaguggguugcaaaagaaguagguagcuuaaccuucggga
gggcgcuuaccacuuugugauucaugacuggggugaagucguaacaaggu
aaccguaggggaaccugcgguuggaucaccuccuua

........<<<<..[.((((.>>>>.<<<<.<<<<<..<<<<<<<<....
.<<<.<<<..<<<..<<.<<..<<<<<<<<..>>>>>.>>>>>..>>>>>
......<<.......<<<<<<<..<<...<<<<<<<.<.<<.....<<<<
<......>>>>>......>>.>.....<<<....>>>....<<<<<<..<
<....>>>>>>>>.>>>>>>>..>>.>>>>>>><<<....<<<..<<<<<
<<.........>>>>>>>>>>......>>>..<<<<<<<<....>>>>..
.>>>>.>>.<<<<<.<.........>>>>>>.<<<<....>>>>...>>>
>>>.........<<<....<<<<....>>>>..>>>..>>.>>>>>>..<
<<<......<<<<....>>>>.....>>>>...<.<<<<<......<.<<
<<<<<<.......>>>>>>>>.>........>>>>>....>..<<<<<((
(...<<<<<.....<<.)))>>.......>>>>>>>>>>..>>>>>>>>>
..........<<<((.....<<<<...<<<.<<<<<<<.<<<<<<<<<<.
.....<<<<<<.....>>>>>>....>>>>>>>>..>>>>>>>>>...<<
<<<<<<...<<<<<<<....<<<<<<<<...<<<......>>>......>
>>>>>>>...........<<....>>.>>>>>>>..>>>>.>>>>...>>
>...>>>>....<<<<<<...<<...<<<<.........>>>>...>>>>
>>>>..........<<<<<<.<<<<<<<<<<<<<.....>>>>>>>>>>>
>>..<<..))>>.....>>>>>>.>>>.<<<......<<<<....>>>>.
...>>>..)))).]<<<<<.<<<<<<<.<<.<<<<<<..<<<<<<<<<<.
.....<<........>>..........<<<<<<<......<<<<<<<..<
<<<<<<....>>>>>>>...<......>..>>>>>.>>.<<<.<<<..<<
<<<<.......<<<<<<<<<....>>>..<<<<......>>>>..>>>>>
>.....<<<<.<<<<<<<...<<..<<<.....>>>>>....>>>>>>>.
....<<<<<.....>>>>>........>>>>.........>>>...>>>>
>>>>>...>>>>>>>...>>.>>>>>>>>.....<<<<<<<.....<<<.
.<<...<<<....>>>...>>....>>>.....>>>>>>>..........
.<<<<<<<........>>>>>>>..........>>>>>>....<<<<<<<
.........>>>>>>>......>>...>>>>>>>>>>.>>....<..<<.
<.<<<<.<<<..<<<<<<<<<<<<....<<<<<<.<<<<..<<....>>.
>>>>>>>>>>...>>>>>>>>>>>>..>>>.>>>>..>.>>...>.....
<<<<<<<<<....>>>>>>>>>..............

Receptor-Ligand Complex Structure

Structure summary

• PDB: 8p18 The compensatory mechanism of a naturally evolved E167K RF2 counteracting the loss of RF1 in bacteria
• Resolution: 2.77 Å
• Binding residue (original residue number in PDB): S2 D21 T22 G23 Q28 H38 L39 H42 H46 K48 D49 H50 H51 S52 R54 R58 K65 Y69 R72
• Binding residue (residue number reindexed from 1): S1 D20 T21 G22 Q27 H37 L38 H41 H45 K47 D48 H49 H50 S51 R53 R57 K64 Y68 R71

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome
• GO:0019843 rRNA binding
• GO:0070181 small ribosomal subunit rRNA binding

Biological Process

• GO:0000028 ribosomal small subunit assembly
• GO:0002181 cytoplasmic translation
• GO:0006412 translation
• GO:0006417 regulation of translation

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0022627 cytosolic small ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:8p18, PDBe:8p18, PDBj:8p18
• PDBsum: 8p18
• UniProt: P0ADZ4|RS15_ECOLI Small ribosomal subunit protein uS15 (Gene Name=rpsO)