Structure of PDB 8jsh Chain t Binding Site BS01

Receptor Information

>8jsh Chain t (length=123) Species: 562 (Escherichia coli)

ATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKKPNSALRK
VCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGA
LDCSGVKDRKQARSKYGVKRPKA

Ligand information

>8jsh Chain g (length=1074)

aauugaagaguuugaucauggcucagauugaacgcuggcggcaggccuaa
cacaugcaagucgaacgguaacaggaagaagcuugcuucuuugcugacga
guggcggacgggugaguaaugucugggaaacugccugauggagggggaua
acuacuggaaacgguagcuaauaccgcauaacgucgcaagaccaaagagg
gggaccuucgggccucuugccaucggaugugcccagaugggauuagcuag
uaggugggguaacggcucaccuaggcgacgaucccuagcuggucugagag
gaugaccagccacacuggaacugagacacgguccagacuccuacgggagg
cagcaguggggaauauugcacaaugggcgcaagccugaugcagccaugcc
gcguguaugaagaaggccuucggguuguaaaguacuuucagcggggagga
agggaguaaaguuaauaccuuugcucauugacguuacccgcagaagaagc
accggcuaacuccgugccagcagccgcgguaauacggagggugcaagcgu
uaaucggaauuacugggcguaaagcgcacgcaggcgguuuguuaagucag
augugaaauccccgggcucaaccugggaacugcaucugauacuggcaagc
uugagucucguagagggggguagaauuccagguguagcggugaaaugcgu
agagaucuggaggaauaccgguggcgaaggcggcccccuggacgaagacu
gacgcucaggugcgaaagcguggggagcaaacaggauuagauacccuggu
aguccacgccguaaacgaugucgacuuggagguugugcccuugaggcgug
gcuuccggagcuaacgcguuaagucgaccgccuggggaguacggccgcaa
gguuaaaacucaaaugaauugccuuguacacaccgcccgucacaccaugg
gaguggguugcaaaagaaguagguagcuuaaccuucgggagggcgcuuac
cacuuugugauucaugacuggggugaagucguaacaagguaaccguaggg
gaaccugcgguuggaucaccuccu

.......<<<<..[.((((.>>>>.<<<<.<<<<<..<<<<<<<<.....
<<<.<<<..<<<..<<.<......<................>.....>..
>>>>>......<<.......<<<<<<<..<<...<<<<<<<.<.<<....
.<.<<........>>.>......>>.>.....<<<....>>>....<<<<
<...<<....>>.>>>>>.>>>>>>>..>>.>>>>>>><<<....<<<..
<<<<<<<.........>>>>>>>>>>......>>>..<<<<<<<<....>
>>>...>>>>.>>.<<<<<.<.........>>>>>>.<<<......>>>.
..>>>>>>.........<<<....<<<<....>>>>..>>>..>>.>>>>
>>..<<<<.......<<<....>>>......>>>>..<<.<<<<<.....
...<<<.<<<<.......>>>>.>>>..........>>>>>....>>.<<
<<<(((...<<<<<.....<<.)))>>.......>>>>>>>>>>..>>>>
>>>>>..........<<<((.....<<<<...<<<.<<<<<<<.<<<<<<
<<<.......<<<<<<.....>>>>>>.....>>>>>>..>>>>>>>>>>
...<<<<<<<<...<<<<<<<....<<<<<<.....<<<......>>>..
......>>>>>>...........<<....>>.>>>>>>>..>>>>.>>>>
...>>>...>>>>....<<<<<<<...<...<<<<.........>>>>..
.>>>>>>>>..........<<<<<<.<<<<<<<....<<.....>>....
>>>>>>>..<<..))>>.....>>>>>>.>>>.<<<......<<<<....
>>>>....>>>..)))).]<.........>.......<<...<<<<...<
.......<..........<<<<<<.<<<<..<<....>>.>>>>>>>>>>
........>........>...>>>>....>>..........<<<<.<<<.
...>>>.>>>>.............

Receptor-Ligand Complex Structure

Structure summary

• PDB: 8jsh Initiation factor 3 bound to the 30S ribosomal subunit in an initial step of translation.
• Resolution: 4.4 Å
• Binding residue (original residue number in PDB): A1 N4 Q5 R11 A12 R13 K17 A25 C26 P27 Q28 K29 R30 N45 S46 A47 L48 R49 G68 E69 K87 D88 R109 K110 Q111 A112 R113 S114 K115 G117 V118 R120 K122
• Binding residue (residue number reindexed from 1): A1 N4 Q5 R11 A12 R13 K17 A25 C26 P27 Q28 K29 R30 N45 S46 A47 L48 R49 G68 E69 K87 D88 R109 K110 Q111 A112 R113 S114 K115 G117 V118 R120 K122

Gene Ontology

Molecular Function

• GO:0000049 tRNA binding
• GO:0003735 structural constituent of ribosome
• GO:0005515 protein binding
• GO:0019843 rRNA binding
• GO:0034336 misfolded RNA binding

Biological Process

• GO:0000372 Group I intron splicing
• GO:0002181 cytoplasmic translation
• GO:0006412 translation
• GO:0033120 positive regulation of RNA splicing
• GO:0034337 RNA folding
• GO:0046677 response to antibiotic
• GO:1990145 maintenance of translational fidelity

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0015935 small ribosomal subunit
• GO:0022627 cytosolic small ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:8jsh, PDBe:8jsh, PDBj:8jsh
• PDBsum: 8jsh
• PubMed: 38148682
• UniProt: P0A7S3|RS12_ECOLI Small ribosomal subunit protein uS12 (Gene Name=rpsL)