Structure of PDB 7zw0 Chain sc Binding Site BS01

Receptor Information

>7zw0 Chain sc (length=144) Species: 580240 (Saccharomyces cerevisiae W303)

GKGKPRGLNSARKLRVHRRNNRWAENNYKKRLLGTAFKSSPFGGSSHAKG
IVLEKLGIESKQPNSAIRKCVRVQLIKNGKKVTAFVPNDGCLNFVDENDE
VLLAGFGRKGKAKGDIPGVRFKVVKVSGVSLLALWKEKKEKPRS

Ligand information

>7zw0 Chain 2 (length=1765)

uaucugguugauccugccaguagucauaugcuugucucaaagauuaagcc
augcaugucuaaguauaagcaauuuauacagugaaacugcgaauggcuca
uuaaaucaguuaucguuuauuugauaguuccuuuacuacaugguauaacu
gugguaauucuagagcuaauacaugcuuaaaaucucgacccuuuggaaga
gauguauuuauuagauaaaaaaucaaugucuucggacucuuugaugauuc
auaauaacuuuucgaaucgcauggccuugugcuggcgaugguucauucaa
auuucugcccuaucaacuuucgaugguaggauaguggccuaccaugguuu
caacggguaacggggaauaaggguucgauuccggagagggagccugagaa
acggcuaccacauccaaggaaggcagcaggcgcgcaaauuacccaauccu
aauucagggagguagugacaauaaauaacgauacagggccggucuuguaa
uuggaaugaguacaauguaaauaccuuaacgaggaacaauuggagggcaa
gucuggugccagcagccgcgguaauuccagcuccaauagcguauauuaaa
guuguugcaguuaaaaagcucguaguugaacuuugggcccgguuggccgg
ucggauuuccaacggggccuuuccuucuggcuaaccuugagcuuggcgaa
ccaggacuuuuacuuugaaaaaauuagaguguucaaagcaggcguauugc
ucgaauauauuagcauggaauaauagaauaggacguuugguucuauuuug
uugguuucuaggaccaucguaaugauuaauagggacggucgggggcauca
guauucaauugucagaggugaaauucuuggauuuauugaagacuaacuac
ugcgaaagcauuugccaaggacguuuucauuaaucaagaacgaaaguuag
gggaucgaagaugaucagauaccgucguagucuuaaccauaaacuaugcc
gacuagggaucgggugguguuuuuuuaaugacccacucggcaccuuacga
gaaaucaaagucuuuggguucuggggggaguauggucgcaaggcugaaac
uuaaaggaauugacggaagggcaccaccaggaguggagccugcggcuuaa
uuugacucaacacggggaaacucaccagguccagacacaauaaggauuga
cagauugagagcucuuucuugauuuugugggugguggugcauggccguuc
uuaguugguggagugauuugucugcuuaauugcgauaacgaacgagaccu
uaaccuacuaaauaguggugcuagcauuugcugguuauccacuucuuaga
gggacuaucgguuucaagccgauggaaguuugaggcaauaacaggucugu
gaugcccuuagacguucugggccgcacgcgcgcuacacugacggagccag
cgagucuaaccuuggccgagaggucuugguaaucuugugaaacuccgucg
ugcuggggauagagcauuguaauuauugcucuucaacgaggaauuccuag
uaagcgcaagucaucagcuugcguugauuacgucccugcccuuuguacac
accgcccgucgcuaguaccgauugaauggcuuagugaggccucaggaucu
gcuuagagaagggggcaacuccaucucagagcggagaauuuggacaaacu
uggucauuuagaggaacuaaaagucguaacaagguuuccguaggugaacc
ugcggaaggaucauu

...<<<<<.{.[[[[>>>>><<<<.<<<<<<...<.<...<......<<<
.<<<..<<....<<....<<..........>>...>>.>>......<<..
......<<<..<<..<<....<<<..................<<....<<
.<<<.....>>>.>>.....>>.........<<<<<..<<....>>..>>
>>><...<<<<<<......(((((((.<<<....>>>....<........
>>>>>>>.....>...<<<<..<<<.....>>>.>>>>....>>>...>>
>>..>>>.<<<....<<<....<<<<<<<<.......>>>>>>>>>>>..
....>>>...<<<.<<<<....>>>>....>>>.>>.<<.<<<.......
...>>>.>>.<.<<....>>.>...>>>>>>.........<<<....<<<
.....>>>..>>>..>....>.>.....<<<<<<<<<.<..>.>>>>>>.
>>>......<<..<...........>..>>.........<<<<<((....
<.<<<<.....<<..))>>.......>>>>>>>>>>..>>>>>>>>>>..
.......<<<[[.....<.<<...<<<.<<....<<<<<<.<<<<.....
.........>>>>.>>>>>>....<<<<<<....................
>>>>>>...<<<<<.<<.......<<...<.......>..<<<.....>>
>....>>......>>.>>..>>>.........<<<..<<<<<<<<.....
))))))).>>>>>>>>.>>>.....>>....<<<<<<.<<...<<<<..<
<..<<<<<<.<...<<<......>>>......>.>>>>>>..>>......
.<<....>>...>>>>...>>>>>.>>>...>>>...>>.>....<<<<<
<<...<...<<<<.<.....>.>>>>...>>>>>>>>..........<<<
.<<.<<<..<<<<<<<<.<<<........>>>>>>>>>>>..>>>...<<
..]]>>...>>.....>>>.>>>.<<<......<<<<....>>>>....>
>>..]]]].}<<<<<.<<<<<<<..<<.<<<<<<..<<<.<<<<<<....
..<<........>>..........<<<<<.<....<<<<<........<<
.<<<........>>>.>>......>>>>>...<<.<<<..<<.<<<<<<.
...<<<.<<<<<....>>>...<<<......>>>...>>.>>>....<<<
<<.<<..<<<<..<<<<<.<<<<<<....>>>>>>...>>>>>..>>>>.
>>....<<<<<<.....>>>>>>.......>>>>>....>>>.>>>....
.>>>>>>>.....>.>>>>>...>>.>>>>.>>>.....<<<<<<<....
..<<.....<<..<<<<....>>>>..>>....>>.......>>>>>>>.
..........<<<<<<..........>>>>>>..........>>>>>>..
..<<<<<<<<.......>>>>>>>>......>>...>>>>>>>>>>.>>.
...<..<<.<..<<<<.<<....<<<<<<<<.<<<..<.<<..<...<<<
<<<<.<<<<.<<<<....>>>>.>>>>.>>>>>>>...>..>>.>..>>>
.>>>>>>>>...>>.>>>>...>.>>...>.....<<<<<<<<<<..>>>
>>>>>>>........

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7zw0 Sensing of individual stalled 80S ribosomes by Fap1 for nonfunctional rRNA turnover.
• Resolution: 2.4 Å
• Binding residue (original residue number in PDB): G2 K3 G4 K5 P6 R7 G8 N10 R13 K14 R19 R20 N22 R23 W24 A25 E26 K30 R32 F38 K39 S46 S47 H48 A49 K50 E60 K62 Q63 N65 S66 A67 R69 K70 K78 P88 N89 D90 L103 G106 G108 R109 K110 K112 K114 G115 D116 R121 S131 L133 K139 K140
• Binding residue (residue number reindexed from 1): G1 K2 G3 K4 P5 R6 G7 N9 R12 K13 R18 R19 N21 R22 W23 A24 E25 K29 R31 F37 K38 S45 S46 H47 A48 K49 E59 K61 Q62 N64 S65 A66 R68 K69 K77 P87 N88 D89 L102 G105 G107 R108 K109 K111 K113 G114 D115 R120 S130 L132 K138 K139

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0000462 maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
• GO:0006412 translation
• GO:0006450 regulation of translational fidelity
• GO:1990145 maintenance of translational fidelity

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0015935 small ribosomal subunit
• GO:0022627 cytosolic small ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:7zw0, PDBe:7zw0, PDBj:7zw0
• PDBsum: 7zw0
• PubMed: 36113412
• UniProt: P0CX29|RS23A_YEAST Small ribosomal subunit protein uS12A (Gene Name=RPS23A)