Structure of PDB 7ohr Chain s Binding Site BS01

Receptor Information

>7ohr Chain s (length=31) Species: 559292 (Saccharomyces cerevisiae S288C)

RQSRRTSTKLKEGIKKKASAHRKKEKKMAKK

Ligand information

>7ohr Chain 1 (length=2373)

guuugaccucaaaucagguaggaguacccgcugaacuuaagcauaucaau
aagcggaggaaaagaaaccaaccgggauugccuuaguaacggcgagugaa
gcggcaaaagcucaaauuugaaaucugguaccuucggugcccgaguugua
auuuggagagggcaacuuuggggccguuccuugucuauguuccuuggaac
aggacgucauagagggugagaaucccguguggcgaggagugcgguucuuu
guaaagugccuucgaagagucgaguuguuugggaaugcagcucuaagugg
gugguuccaucuaaagcuaaauauuggcgagagaccgauagcgaacaagu
acagugauggaaagaugaaaagaacuuugaaaagagagugaaaaaguacg
ugaaauuguugaaagggaagggcauuugaucagacaggccagcaucaguu
uugguggcaggauaaauccauaggaauguagcuugccucgguaaguauua
uagccugugggaauacugccagcugggacugaggacugcgacguaaguca
aggaugcuggcauaaugguuauaugccgcccgucuugaaacacggaccaa
ggagucuaacgucuaugcgaguguuuggguguaaaacccauacgcguaau
gaacgagguuggggccucgcaagaggugcacaaucgaccgauccugagga
uggauuugguaagagcauagcuguugggacccgaaagauggugaacuaug
ccugaauagggugaagccagaggaaacucugguggagcucgguucgacgu
ucgaucgugaauuggguauagggcgaaagacaaccaucuaguagcugguu
ccugccgaaguuucccuaggauagcagaagcucguaucaguuuuaugagu
aagaaugaagagcuuuuagugggccauuuuugguaagcagaacuggcgau
gcgggaugaaccgaacguagaguuaaggugccggaauacacgcucaucag
acacaaaagguguuaguucaucuagacagccggacgguggccauggaagu
cggaauccgcuaaggaguguguaacaacucaccggccgaaugaacuagcc
cugaaaauggauggcgcucaagcguguuaccuauacucuaccgucagggu
ugauaugaugcccugacgaguaggcaggcguggaggucagugacgaagcc
uagaccguaaggucgggucgaacggccucuagugcagaucuuggugguag
uagcaaauauucaaaugagaacuuugaagacugaaguggggaaagguucc
acgucaacagcaguuggacguggguuagucgauccuaagagauggggaag
cuccguuucaaaggccugauuuuaugcaggccaccaucgaaagggaaucc
gguuaagauuccggaaccuggauauggauucuucacgguaacguaacuga
auguggagacgucggcgcgagcccugggaggaguuaucuuuucuucuuaa
cagcuuaucaccccggaauugguuuauccggagauggggucuuauggcug
gaagaggccagcacugcuggcuccggugcgcuugugacggccgugaaaau
ccacaggaaggaauaguuuucaugccaggucguacugggucuccaaggug
aacagccucuaguugauagaauaauguagauaagggaagucggcuguccc
uaucuacuaucuagcgaaaccacagccaagggaacgggcuuggcagaauc
agcggggacccuguugagcuugacucuaguuugacauuauugucaggugg
ggaguaaaguuaccacagggauaauggcuuguggcagucaagcguucagc
gacauugcucggcucuuccuaucauaccgaagcagaauucgguaagcguu
ggauuguucacccacuaauagggaacguggcuggguagaccgucgugaga
cgguuaguuuuacccuacugaugaauguuaccgcaauaguaauugaacuu
aguacgagaggaacaguucauucggauaauugguuuuugcggcugucuga
ucaggcaugccgcgaagcuaccauccgcuggauuauggcugaacgccucu
aagucagaauccaugcuagaacgcggugauuucuuugcuccacacaauau
agauggauacgaauaaggcguccuuguggcgucgcugaaccauagcaggc
uagcaacggugcacuuggcggaaaggccuugggugcuugcuggcgaauug
caaugucauuuugcguggggauaaaucauuuguauacgacuuagauguac
aacgggguauuguaagcaguagaguagccuugguuacgaucugcugagau
uaagccuuuguugucugauuugu

..........................<<<<<<.....<......>.....
.>>>>>.>...<.....<<....>>..<<<<<......<<.....>>...
..>>>>>..<<<..<........<<.<<<<<<...>>>>>>.>>......
..>....<<<<<<<.<<<<<<<<<<<<<<<<<<<<.....<<<<......
>>>>.((......<<<.....))>>>....>>>>>>>>>..>>>>>>>..
..>>>>>>>>>>>........<<<<<<<........>>>>>>>.....<<
<<<<..>>>>>>..>>>....>............................
.<<....>>...............<<<<....>>>>..............
....................<<<<<.<<<<<......<<<<<<<.<<<<<
<<<<<<<<<<.....<<<<<<<<<......<<<<<<....>>>>>>....
...>>>>>>>.>.>.>>>>>.>>>>>>>>>>........<<<....>>>.
....>>>>>>>.....>>>>>.>>>>>..<<<<........>>>><<<<<
<....<<<<<<.<<<<<<..<<<...<<<<.....>>>>...>>>.....
......<<<<<..<<<<<....>>>>>...>>>>>.<<<<<<<<......
.>>>.>>>>>....>>>>>>.>>>>>>..<<.<..<<<<<<<...<<<<<
<<<<....<<<<...<<<<<<<....>>>>>>>...>>>>...<<<....
.>>>........>>>>>>>>>...........>>>>>>>.....>.>>..
<<<<<<....<...........><<<<<<<<<....<<<.<<<<<<..>>
>>.>>>>>..>>>>>>>.>>..<<<<.<<<<<.....>>>>>.>>>>...
.<<<......>>>...<<<<<<<..<<<<(((.....<<<<<<.....<<
.......<<<.<<<<<<<<<<.......<<<<<.<<<...<<........
.>>...>>>.........<<......>>...>>>>>...>>>>>>>>>>>
>>..........))).>>...>>>>>>.>>>>..>>>>>>><<<<<<<<<
.........>>>>>>>>>....>>>>>>..<<<<<<<..<<......<<<
<.<<<<....>>>>>>>>...>>.>>>>>>>........>>>>>><<...
..<<.....<<<<<<.......>>>>>><<<<.....<<<<<.<<...<<
<<<<...<<.....>>.>>>>>>....((<<<<<<...<<....<<<...
.>>>........<<<<<<........>>>>>>....>>....>>...<<<
<<........>>>>>))<<<<.<<<<<<<.....<<<....>>>...<..
.<<<<<<...<<<<.<<<<<<<.<<<<<<<<<<..(((..>>>>>><...
<<<<<....<<<<<<....<<<.....>>>....>>>>>>.....>>>>>
....><<<<<<<...>>>>>>>>>>>...>>>>>>>.>>>>........>
>>>>>...>......>.>>.>>>>>>>>>>>>..>>...>>>>>.<<<<.
....>>>>..>>>>..<<<<.....<<<<<<<.<<............>>.
>>>>>>>..>>>>>>....>>....<<<<<........>>>>>....<<<
<<..<<..<<<<<.<..<<<<..<<<<.<<<<<<<<....>>>>>>>>..
>>>>..>>>>....>>>>>>............<<<<<....<<.....>>
....>>>>>.<<...<<<<<..<.<<<<<<<......>>>>>>>...>.<
<<.........>>>.....>>>>>......>>.....<<<<<<<....>>
>>>>>.........>>.>>>>>....<<<<<<<<..<<<<.<.<<<<<<.
..............>>>>>>.><<<<<...<<<<...<<<<<<.<<<<))
).>>>>..>>>>>>..>>>>..>>>>>.<<<<...<<<<<..........
.>>>>>...>>>>.>>>>....>>>>>>>>.......<<<<<<.<<<.<<
....<<..........<<<<<<.....>>>>>>......>>.......<<
<<<<...<<<<<.<..<<<......>>>..>.>>>>>.>>>>>>......
.......>>.>>>.>>>>>>..<<<<<.<.............>.....<<
<<<.<<<.<<<....<<<<....<..<<<..>>>..>...>>>>...>>>
....>>>..>>>>>..>>>>>..

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7ohr Analysis of subunit folding contribution of three yeast large ribosomal subunit proteins required for stabilisation and processing of intermediate nuclear rRNA precursors.
• Resolution: 4.72 Å
• Binding residue (original residue number in PDB): R6 Q7 S8 R9 R10 T11 T13 K14 L15 E17 G18 I19 K21 K22 A23 S24 A25 H26 K28 K32
• Binding residue (residue number reindexed from 1): R1 Q2 S3 R4 R5 T6 T8 K9 L10 E12 G13 I14 K16 K17 A18 S19 A20 H21 K23 K27

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003729 mRNA binding
• GO:0003924 GTPase activity
• GO:0005515 protein binding
• GO:0005525 GTP binding

Biological Process

• GO:0000055 ribosomal large subunit export from nucleus
• GO:0000463 maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
• GO:0015031 protein transport
• GO:0042254 ribosome biogenesis

Cellular Component

• GO:0005634 nucleus
• GO:0005730 nucleolus
• GO:0030687 preribosome, large subunit precursor

External links

• PDB: RCSB:7ohr, PDBe:7ohr, PDBj:7ohr
• PDBsum: 7ohr
• PubMed: 34813592
• UniProt: P40010|NUG1_YEAST Nuclear GTP-binding protein NUG1 (Gene Name=NUG1)