Structure of PDB 6n8o Chain r Binding Site BS01

Receptor Information

>6n8o Chain r (length=126) Species: 559292 (Saccharomyces cerevisiae S288C)

ASLPHPKIVKKHTKKFKRHHSDRYHRVAENWRKQKGIDSVVRRRFRGNIS
QPKIGYGSNKKTKFLSPSGHKTFLVANVKDLETLTMHTKTYAAEIAHNIS
AKNRVVILARAKALGIKVTNPKGRLA

Ligand information

>6n8o Chain A (length=3201)

uugaccucaaaucagguaggaguacccgcugaacuuaagcauaucaauaa
gcggaggaaaagaaaccaaccgggauugccuuaguaacggcgagugaagc
ggcaaaagcucaaauuugaaaucugguaccuucggugcccgaguuguaau
uuggagagggcaacuuuggggccguuccuugucuauguuccuuggaacag
gacgucauagagggugagaaucccguguggcgaggagugcgguucuuugu
aaagugccuucgaagagucgaguuguuugggaaugcagcucuaagugggu
gguaaauuccaucuaaagcuaaauauuggcgagagaccgauagcgaacaa
guacagugauggaaagaugaaaagaacuuugaaaagagagugaaaaagua
cgugaaauuguugaaagggaagggcauuugaucagacggccagcaucagu
uuugguggcaggauaaauccauaggaauguagcuugccucgguaaguauu
auagccugugggaauacugccagcugggacugaggacugcgacguaaguc
aaggaugcuggcauaaugguuauaugccgcccgucuugaaacacggacca
aggagucuaacgucuaugcgaguguuuggguguaaaacccauacgcguaa
ugaaagugaacguagguuggggccucgcaagaggugcacaaucgaccgau
ccugaugucuucggauggauuugaguaagagcauagcuguugggacccga
aagauggugaacuaugccugaauagggugaagccagaggaaacucuggug
gaggcucguagcgguucugacgugcaaaucgaucgucgaauuuggguaua
ggggcgaaagacuaaucgaaccaucuaguagcugguuccugccgaaguuu
cccucaggauagcagaagcucguaucaguuuuaugagguaaagcgaauga
uuagagguuccggggucgaaaugaccuugaccuauucucaaacuuuaaau
auguaagaaguccuuguuacuuaauugaacguggacauuugaaugaagag
cuuuuagugggccauuuuugguaagcagaacuggcgaugcgggaugaacc
gaacguagaguuaaggugccggaauacacgcucaucagacaccacaaaag
guguuaguucaucuagacagccggacgguggccauggaagucggaauccg
cuaaggaguguguaacaacucaccggccgaaugaacuagcccugaaaaug
gauggcgcucaagcguguuaccuauacucuaccgucaggguugauaugau
gcccugacgaguaggcaggcguggaggucagugacgaagccuagaccgua
aggucgggucgaacggccucuagugcagaucuuggugguaguagcaaaua
uucaaaugagaacuuugaagacugaaguggggaaagguuccacgucaaca
gcaguuggacguggguuagucgauccuaagagauggggaagcuccguuuc
aaaggccugauuuuaugcaggccaccaucgaaagggaauccgguuaagau
uccggaaccuggauauggauucuucacgguaacguaacugaauguggaga
cgucggcgcgagcccugggaggaguuaucuuuucuucuuaacagcuuauc
accccggaauugguuuauccggagauggggucuuauggcuggaagaggcc
agcaccuuugcuggcuccggugcgcuugugacggcccgugaaaauccaca
ggaaggaauaguuuucaugccaggucguacugauaaccgcagcaggucuc
caaggugaacagccucuaguugauagaauaauguagauaagggaagucgg
caaaauagauccguaacuucgggauaaggauuggcucuaagggucggacg
aucaacuuagaacugguacggacaaggggaaucugacugucuaauuaaaa
cauagcauugcgauggucagaaagugauguugacgcaaugugauuucugc
ccagugcucugaaugucaaagugaagaaauucaaccaagcgcggguaaac
ggcgggaguaacuaugacucucuuaagguagccaaaugccucgucaucua
auuagugacgcgcaugaauggauuaacgagauucccacugucccuaucua
cuaucuagcgaaaccacagccaagggaacgggcuuggcagaaucagcggg
gaaagaagacccuguugagcuugacucuaguuugacauugugaagagaca
uagaggguguagaauaagugggagcuucggcgccagugaaauaccacuac
cuuuauaguuucuuuacuuauucaaugaagcggagcuggaauucauuuuc
cacguucuagcauucaaggucccauucggggcugauccggguugaagaca
uugucagguggggaguuuggcuggggcggcacaucuguuaaacgauaacg
cagauguccuaaggggggcucauggagaacagaaaucuccaguagaacaa
aaggguaaaagcccccuugauuuugauuuucagugugaauacaaaccaug
aaaguguggccuaucgauccuuuagucccucggaauuugaggcuagaggu
gccagaaaaguuaccacagggauaacuggcuuguggcagucaagcguuca
uagcgacauugcuuuuugauucuucgaugucggcucuuccuaucauaccg
aagcagaauucgguaagcguuggauuguucacccacuaauagggaacgug
agcuggguuuagaccgucgugagacagguuaguuuuacccuacugaugaa
uguuaccgcaauaguaauugaacuuaguacgagaggaacaguucauucgg
auaauugguuuuugcggcugucugaucaggcauugccgcgaagcuaccau
ccgcuggauuauggcugaacgccucuaagucagaauccaugcuagaacgc
ggugauuucuuugcuccacacaauauagauggauacgaauaaggcguccu
uguggcgucgcugaaccauagcaggcuagcaacggugcacuuggcggaaa
ggccuugggugcuugcuggcgaauugcaaugucauuuugcguggggauaa
aucauuuguauacgacuuagauguacaacgggguauuguaagcaguagag
uagccuuguuguuacgaucugcugagauuaagccuuuguugucugauuug
u

........................<<<<<<.....<......>......>
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>>>>>..[[[..<<.......<<.<<<<<<...>>>>>>.>>.......>
>....<<<<<<.<<<<<<.<<<<<<<<<<<<<<<....<<<<......>>
>>.(.......<<<......)>>>..>.>>>>>>>>..>>>>>>.>....
>>>>>>>>>>>........<<<<<<<........>>>>>>>.....((((
(..<<<<<<)))))..]]]....}..........................
...<<....>>...............<<<<....>>>>............
......................<<<<<.<<<<<.....<<<<<<<.<<<<
<<<<<<<<<<<.....<<<<<<<<<......<<<..<....>..>>>...
....>>>>>>>.>.>.>>>>>.>>>>>>>>>>........<<<....>>>
.....>>>>>>>.....>>>>>.>>>>>..<<<<........>>>><<<<
<<.....<<<<<.<<<<<<..<<<...<<<<.....>>>>...>>>...<
<....>>.......<<<<<..<<<<<....>>>>>...>>>>>.<<<<<<
<<...<<....>>...>>>.>>>.>>....>>>>>>.>>>>>...<<.<.
.<<<<<<<.....<<<<<<<<<..<<<....<<<<<<<....>>>>>>>.
....>>>...<<<..<<.<<........>>>>.>>>....>>>>>>>>>.
..<<.......>>......>>>>>>...>..>.>>..<<<<<<....<.<
<<....>>>.><<<<<<<<<<...<<<.<<<<<<<.....<<<....<<<
....<<<<...<<<<<<.....>>>>>>.>>>>....>>>..>.>>....
.>>>>>...<<<<.<<<<.<......>>>>>.>>>>.....>>>>>.>>>
>>>>>.>>..<<<<.<<<<<.....>>>>>.>>>>....<<<......>>
>...<<<<<<<..<<<<(((.....<<<<<<.....<<..........<<
<.<<<<<<<<<<.......<<<<<.<<<...<<.........>>...>>>
.........<<......>>...>>>>>...>>>>>>>>>>>>>.......
...))).>>...>>>>>>.>>>>..>>>>>>><<<<<<<<<.........
>>>>>>>>>....>>>>>><.<<<<<<<<.<<......<<<<.<<<<...
.>>>>>>>>...>>>>>>>>>>..>.....>>>>>><<.....<<.....
<<<<<<.......>>>>>><<<<.....<<<<<.<<...<<<<<<...<<
.....>>.>>>>>>....((<<<<<<...<<....<<<....>>>.....
...<<<<<<........>>>>>>....>>....>>...<<<<<.......
.>>>>>))<<<<.<<<<<<<.....<<<....>>>.......<<<<<<..
.<<<<.<<<<<<<<<<<<<<<<<<..{{{..>>>>>>....<<<<<....
<<<<<<....<<<.....>>>....>>>>>>.....>>>>>.....<<<<
<<<......>>>>>>>>>>>.>.>>>>>>>.>>>>.........>>>>>>
..........>>>..>>>>>>>>>>>>..>>.....<<......>>>>>>
>.<<<<.....>>>>..>>>>..<<<<.....<<<<<<<.<<........
.<.....<<<<<...............>>>>><<.<<<<<<<<<<<..>>
>>>..>>>>>>.>>............<<<<<<<<<..<<<.........>
>>..<<<<<<<....<<<<.....>>>>......>>>>>>>.<.<<..<<
<..<<<<<..<..<<<....<<<<<....>>>>.>...>>>>.>>>..>>
>>><<<<<<.......>>>>>>........<...........<<<<<...
....>>>>>.....>.....>>.>..>>.>>>>>>>..>..>>.>>>>>>
>..>>>>>>....>>....<<<<<........>>>>>....[[[[[..{{
{.{{......[[[[.{{[[[[..<<<<<.<<<<<<<<..<<<<<<<<<<<
<<<<<............<<<<......................>>>>...
>>>>>>.>>>>>>>>>>...<<<<<.....<<<<..<<<<........>>
>>...............<<<<<<....>>>>>>..>>>>..>>>>>....
.>>>>>>>>..>>>>>...<<<.....>>>..<<<<<<...........>
>>>>>..<<...<<<<<<<...<<<<<<.......>>>>>>.....<<..
.(((>>....>>>>>>>...<<<.<<<<<.......>>>>.>>>>.<<<.
...>>>.>>))).......((((((..((((.>>>>>>))))))))))..
........]]]]..}}]]]].......<<.<.<.<<<<<<...<<.....
..>>...>>>>>>...>>.>.>.......<<<<<.<<<<<<..<.<<<<<
<<......>>>>>>>...>.<<<.........>>>.....>>>>>..>..
.>>>.>>....<<<<<<<....>>>.>>>>....}}..}}}.]]]]]...
.<<<<<<<<..<<<<.<.<<<<<<...............>>>>>>.><<<
<<...<<<<....<<<<<<<<<<}}}.>>>>>..>>>>>...>>>>..>>
>>>.<<<<...<<<<<...........>>>>>...>>>>.>>>>....>>
>>>>>>.......<<<<<<.<<<.<<....<<..........<<<<<<..
...>>>>>>......>>.......<<<<<<...<<<<<.<..<<<.....
.>>>..>.>>>>>.>>>>>>.............>>.>>>.>>>>>>..<<
<<<.<.............>.....<<<<<<<<<.<<<....<<<<.<..<
<<<<......>>>>>..>>>>>...>>>....>>>>.>>>>>..>>>>>.
.

Receptor-Ligand Complex Structure

Structure summary

• PDB: 6n8o Tightly-orchestrated rearrangements govern catalytic center assembly of the ribosome.
• Resolution: 3.5 Å
• Binding residue (original residue number in PDB): K11 K12 K15 K16 F17 R19 H21 D23 R24 Y25 H26 R27 N31 W32 R33 K34 K36 G37 I38 D39 S40 R43 R44 R45 R47 P53 K54 I55 G56 Y57 G58 S59 N60 K61 K64 F65 L66 P68 S69 L75 N78 E95 A97 H98 N99 I100 S101 R105 N121 R125
• Binding residue (residue number reindexed from 1): K10 K11 K14 K15 F16 R18 H20 D22 R23 Y24 H25 R26 N30 W31 R32 K33 K35 G36 I37 D38 S39 R42 R43 R44 R46 P52 K53 I54 G55 Y56 G57 S58 N59 K60 K63 F64 L65 P67 S68 L74 N77 E94 A96 H97 N98 I99 S100 R104 N120 R124

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0002181 cytoplasmic translation
• GO:0006412 translation

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:6n8o, PDBe:6n8o, PDBj:6n8o
• PDBsum: 6n8o
• PubMed: 30814529
• UniProt: P38061|RL32_YEAST Large ribosomal subunit protein eL32 (Gene Name=RPL32)