Structure of PDB 6tnn Chain p Binding Site BS01

Receptor Information

>6tnn Chain p (length=54) Species: 224308 (Bacillus subtilis subsp. subtilis str. 168)

AVPFRRTSKMKKRLRRTHFKLNVPGMTECPSCGEMKLSHRVCKACGSYNG
KDIN

Ligand information

>6tnn Chain U (length=2930)

acgguuaaguuagaaagggcgcacgguggaugccuuggcacuaggagccg
augaaggacgggacgaacaccgauaugcuucggggagcuguaagcaagcu
uugauccggagauuuccgaauggggaaacccaccacucguaauggagugg
uauccauaucugaauucauaggauaugagaaggcagacccggggaacuga
aacaucuaaguacccggaggaagagaaagcaaaugcgauucccugaguag
cggcgagcgaaacgggaucagcccaaaccaagaggcuugccucuuggggu
uguaggacacucuguacggaguuacaaaggaacgagguagaugaagaggu
cuggaaaggcccgccauaggagguaacagcccuguagucaaaacuucguu
cucuccugaguggauccugaguacggcggaacacgugaaauuccgucgga
auccgggaggaccaucucccaaggcuaaauacucccuagugaccgauagu
gaaccaguaccgugagggaaaggugaaaagcaccccggaaggggagugaa
agagauccugaaaccgugugccuacaaguagucagagcccguuaacgggu
gauggcgugccuuuuguagaaugaaccggcgaguuacgaucccgugcaag
guuaagcagaagaugcggagccgcagcgaaagcgagucugaauagggcgc
augaguacguggucguagacccgaaaccaggugaucuacccauguccagg
gugaaguucagguaacacugaauggaggcccgaacccacgcacguugaaa
agugcggggaugagguguggguaggggugaaaugccaaucgaaccuggag
auagcugguucucuccgaaauagcuuuagggcuagccucaagguaagagu
cuuggagguagagcacugauuggacuaggggccccuaccggguuaccgaa
uucagucaaacuccgaaugccaaugacuuauccuugggagucagacugcg
agugauaagauccguagucgaaagggaaacagcccagaccgccagcuaag
gucccaaaguauacguuaaguggaaaaggauguggaguugcuuagacaac
caggauguuggcuuagaagcagccaccauuuaaagagugcguaauagcuc
acuggucgagugacucugcgccgaaaauguaccggggcuaaacguaucac
cgaagcugcggacuguucuucgaacagugguaggagagcguucuaagggc
ugugaagccagaccggaaggacugguggagcgcuuagaagugagaaugcc
gguaugaguagcgaaagaggggugagaauccccuccaccgaaugccuaag
guuuccugaggaaggcucguccgcucaggguuagucgggaccuaagccga
ggccgaaaggcguaggcgauggacaacagguugauauuccuguaccaccu
ccucaccauuugagcaauggggggacgcaggaggauaggguaagcgcggu
auuggauauccgcguccaagcaguuaggcugggaaauaggcaaauccguu
ucccauaaggcugagcugugauggcgagcgaaauauaguagcgaaguucc
ugauuccacacugccaagaaaagccucuagcgaggugagaggugcccgua
ccgcaaaccgacacagguaggcgaggagagaauccuaaggugaucgagag
aacucucguuaaggaacucggcaaaaugaccccguaacuucgggagaagg
ggugcucuguuagggugcaagcccgagagagccgcagugaauaggcccag
gcgacuguuuagcaaaaacacaggucucugcgaagccguaaggcgaagua
uaggggcugacgccugcccggugcuggaagguuaagaggagcgcuuagcg
uaagcgaaggugcgaauugaagccccaguaaacggcggccguaacuauaa
cgguccuaagguagcgaaauuccuugucggguaaguuccgacccgcacga
aaggcgcaacgaucugggcacugucucaacgagagacucggugaaauuau
aguaccugugaagaugcagguuacccgcgacaggacggaaagaccccgug
gagcuuuacugcagccugauauugaauguugguacagcuuguacaggaua
gguaggagccuuggaaaccggagcgccagcuuugguggaggcaucggugg
gauacuacccuggcuguauugaccuucuaacccgccgcccuuaucgggcg
gggagacagugucaggugggcaguuugacuggggcggucgccuccuaaaa
gguaacggaggcgcccaaagguucccucagaaugguuggaaaucauucgc
agaguguaaaggcacaagggagcuugacugcgagaccuacaagucgagca
gggacgaaagucgggcuuagugauccggugguuccgcauggaagggccau
cgcucaacggauaaaagcuaccccggggauaacaggcuuaucucccccaa
gaguccacaucgacggggagguuuggcaccucgaugucggcucaucgcau
ccuggggcuguagucggucccaaggguugggcuguucgcccauuaaagcg
guacgcgagcuggguucagaacgucgugagacaguucggucccuauccgu
cgcgggcgcaggaaauuugagaggagcuguccuuaguacgagaggaccgg
gauggacgcaccgcugguguaccaguuguucugccaagggcaucgcuggg
uagcuaugugcggacgggauaagugcugaaagcaucuaagcaugaagccc
cccucaagaugagauuucccauuccgcaaggaaguaagaucccugaaaga
ugaucagguugauaggucugagguggaaguguggcgacacauggagcuga
cagauacuaaucgaucgaggacuuaaccau

..<<<<<<<<......<<<<<<<<<<<....{[[..[[<<<<<<<[[<<.
.....<<<<<<.....((.>>>.....<<<<<<<..<<.))......>>.
....>>>>>>>...>>>....<<<....>>><<<<<<<......>>>>>>
>.<<<<<<<<<.........>>>>>>>.>>.>>....<<<<<<.....<<
....>>......>>>>>.>.........<<....>>...<<<<......<
<.....>>.....>>>>...(((....<<<<<<<<....>>>>>>>>...
...<<<<<<<<<.....<<<<.......<<<<<<<<<<.<<<.....<<<
<......>>>>.((......<<<.....))>>>...>>>...>>>>>>>>
>>>>>>.>>>>>..>>>>.....<<<<<<<<.........>>>>>>>>..
....<<<<<..<<<<>>>>>..)))......]].>>>>>>>.]]......
.........<<....>>....]].........<<<<....>>>>......
.......}....>>>>>>>>>>>.......<<<<..<<<<......>>>>
..>>>>...<.<........>.>.<<<<<<....<<<<<<<.<<<<<..<
<<...<<<.....>>>...>>>...<<....>>..<<<<.....>>>>..
....>>>>>.>>>>>>>..<<.<..<<<<<<<.....<<<<<<<<<..<<
<....<<<<<<......>>>>>>.....>>>...<<<.<<<<<.......
.>>>>>>>>....>>>>>>>>>...<<<.....>>>......>>>>>>..
.>..>.>>..<<<<<<....<<<<<....>>>>><<<<<<<<<...<<<<
<<<<<.....<<<...<<<<<<<<......<<<<.....>>>>.......
>>>>>>>>..>>.>.....>>>>.>>>>>..>>>>>>>.>>..<<<<<<<
.<<......>>.>>>>>>>....<<<......>>>...<<<<.<<<...<
<<(((.....<<<<<<...........<<..<<<<<<<<<........<<
<<<.<<.....................>>.........<<......>>..
.>>>>>....>>>>>>>>>.>>...........))).....>>>>>>.>>
>...>>>>>>><<<<<<<...>>>>>>>....>>>>>><.<<<<<<<.<<
......<<<<<.<<....>>.>>>>>...>>.>>>>>>>..>.....>>>
>>><......<<....<<<<<<.......>>>>>><<<<....<<<<<.<
<<..<<<<<<...<<.....>>.>>>>>>....((<<<<.<<...<<<..
.<<<....>>>...>>>...>>...<<<<<........>>>>>))<<<<<
.<<<<<<......<<...<<<<....<<<<..<<<..<<<.<.<<<.<..
...{....>.>>>.<<..<......<<.<.<<<<<<<.<<.....>>>>>
>>>>.....>>>......>...>><..<<...........>>...>>.>>
>...>>>...>>>>.........>>>>..>>..>>>>>>>>>>>>>>>.>
>>.....<<......>>>>>>>.<<<<.....>>>>..>>>>..<<<<..
..<<<<<<<<.<<<........<.....<<<<<....<<..>>.....>>
>>><<<<<....<<<<....>>>>...>>>>>............<<<<<<
<<<..<<<.........>>>..<<<<<<<.....<<<....>>>......
.>>>>>>>..<<<<.<<<..<<<<<<<..<<<...<<...<<<<<..<<.
...>>...>>>>>...>>...>>>>>>>>..>>>>><<<<<<.......>
>>>>>........<...........<<<<<.......>>>>>.....>..
..>>>>...>>.>>>>>>>..>.>>>.>>>>>>>>.>>>>>>.....>..
...<<<<<........>>>>>..<<<<<<<<.(((.[[......<<<<.[
[<<<<..<<<<<.<<<<<<<<<.<<<.<<<..<<<<<<............
<<<<<..<<...............................>>........
....>>>>>..>.>>>>>..>>>.>>>....<<.<<<<<.......>>>>
>>>.....>>>>>>>>>..>>>>>..<<<<.....>>>><<<<<<.....
......>>>>>>..<<...<<<<<<<...<<<<<<.......>>>>>>..
...<<.[.(((>>....>>>>>>>...<<<<..<<<......>>>..>>>
>.<<<....>>>.>>)))]......<<<<<<<.((..>>>>..))>>>>>
>>.............>>>>..]]>>>>.......<<<<...<<<<<<...
<<.......>>...>>>>>>....>>>.>.......<<<<<.<<<<<<..
<<<<<<<<<......>>>>>>..>>>.<<<<<.....>>>>>.....>>>
>>..>...>>>.>>....<<<<<<<....>>>.>>>>....]]..)))>>
>>>>>>....<<<<<<<...<<<<..<<<<<<<...............>>
>>>>>.<<<<<...<<<<...<<<<<<<<<<<.}..>>>>>>..>>>>>.
..>>>>.>>>>>...<<<....<<<<<...........>>>>>....>>>
.>>>>.......>>>>>>>..<<<<....>>>>....<<<<<<<<<....
...>>>>>....<<<<<<<<.<<<....<<<<<....>>>>>...>>>..
>>>>>.>>>...>>>>....>>>>>>>>..

Receptor-Ligand Complex Structure

Structure summary

• PDB: 6tnn Structures of B. subtilis Maturation RNases Captured on 50S Ribosome with Pre-rRNAs.
• Resolution: 3.07 Å
• Binding residue (original residue number in PDB): A2 V3 P4 F5 R6 R7 T8 S9 K10 K12 K13 R16 R17 T18 H19 T28 H40 Y49
• Binding residue (residue number reindexed from 1): A1 V2 P3 F4 R5 R6 T7 S8 K9 K11 K12 R15 R16 T17 H18 T27 H39 Y48

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0006412 translation

Cellular Component

• GO:0005840 ribosome
• GO:0015934 large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:6tnn, PDBe:6tnn, PDBj:6tnn
• PDBsum: 6tnn
• PubMed: 32991829
• UniProt: O34687|RL32_BACSU Large ribosomal subunit protein bL32 (Gene Name=rpmF)