Structure of PDB 5it7 Chain oo Binding Site BS01

Receptor Information

>5it7 Chain oo (length=101) Species: 28985 (Kluyveromyces lactis)

VNVPKTRKTYCKGKECRKHAQHKVTQYKAGKASLYAQGKRRYDRKQSGFG
GQTKQIFHKKAKTTKKVVLRLECMSCKTKTQLALKRCKHFELGGEKKQKG
Q

Ligand information

>5it7 Chain 5 (length=3270)

uugaccucaaaucagguaggaauacccgcugaacuuaagcauaucaauaa
gcggaggaaaagaaaccaaccgggauugccuuaguaacggcgagugaagc
ggcaaaagcucaaauuugaaaucuggcgucuucgacguccgaguuguaau
uugaagaaggcaacuuuguagcugguccuugucuauguuccuuggaacag
gacgucauagagggugagaaucccguguggcgaggaucccaguuauuugu
aaagugcuuucgacgagucgaguuguuugggaaugcagcucuaagugggu
gguaaauuccaucuaaagcuaaauauuggcgagagaccgauagcgaacaa
guacagugauggaaagaugaaaagaacuuugaaaagagagugaaaaagua
cgugaaauuguugaaagggaagggcauuugaucagacaugggccagcauc
aguuuuagcgguuggauaaauccucgggaauguggcucugcuucgguaga
guguuauagcccgugggaauacagccagcugggacugaggauugcgacuu
uugucaaggaugcuggcguaaugguuaaaugccgcccgucuugaaacacg
gaccaaggagucuaacgucuaugcgaguguuuggguguaaaacccguacg
cguaaugaaagugaacguaggugagggcccgcaagggugcaucaucgacc
gauccugaugucuucggauggauuugaguaagagcauagcuguugggacc
cgaaagauggugaacuaugccugaauagggugaagccagaggaaacucug
guggaggcucguagcgguucugacgugcaaaucgaucgucgaauuugggu
auaggggcgaaagacuaaucgaaccaucuaguagcugguuccugccgaag
uuucccucaggauagcagaagcucguaucaguuuuaugagguaaagcgaa
ugauuagagguaccgggguugaaaugaccuugaccuauucucaaacuuua
aauauguaagaaguccuuguugcuuaauugaacguggacauuugaaugaa
gagcuuuuagugggccauuuuugguaagcagaacuggcgaugcgggauga
accgaacguggaguuaaggugccggaauacacgcucaucagacaccacaa
aagguguuaguucaucuagacagccggacgguggccauggaagucggaau
ccgcuaaggaguguguaacaacucaccggccgaaugaacuagcccugaaa
auggauggcgcucaagcguguuaccuauacuccaccgucaggguuaauau
gaugcccugacgaguaggcaggcguggaggucagugacgaagccuaggcu
guaaagcuggguagaacggccucuagugcagaucuuggugguaguagcaa
auauucaaaugagaacuuugaagacugaaguggggaaagguuccacguca
acagcaguuggacguggguuagucgauccuaagagauggggaagcuccgu
uucaaaggccuaauuuucuaggccaccaucgaaagggaauccgguuaaua
uuccggaaccuggauauggauucuucacgguaacguaacugaauguggag
acgucggcgcgagcccugggaggaguuaucuuuucuucuuaacagcuuau
caccccggaauugguuuauccggagagggggucuuauggcuggaagagcc
cagcccuugugcuggguccggugcgcccgcgacggcccuugaaaauccac
aggaaggaauaguuuucaugccaggucguacugauaaccgcagcaggucu
ccaaggugaacagccucuaguugauagaauaauguagauaagggaagucg
gcaaaauagauccguaacuucgggauaaggauuggcucuaaggaucgggc
gugagggccuuggucagacgcggcguuguagacggccuugguaggucucu
uguagaugcugcaauuaacgaucaacuuagaacugguacggacaagggga
aucugacugucuaauuaaaacauagcauugcgauggucagaaagugaugu
ugacgcaaugugauuucugcccagugcucugaaugucaaagugaagaaau
ucaaccaagcgcggguaaacggcgggaguaacuaugacucucuuaaggua
gccaaaugccucgucaucuaauuagugacgcgcaugaauggauuaacgag
auucccacugucccuaucuacuaucuagcgaaaccacagccaagggaacg
ggcuuggcagaaucagcggggaaagaagacccuguugagcuugacucuag
uuugacauugugaagagacauagaggguguagcauaagugggagcuucgg
cgccagugaaauaccacuaccuuuauaguuucuuuacuuauucaauuaag
cggagcuggaauucauuuuccacguucuagcauucaaaguccuauacggg
cugauccggguugaagacauugucagguggggaguuuggcuggggcggca
caucuguuaaacgauaacgcagauguccuaagggggacucauggagaaca
gaaaucuccaguagaacaaaaggguaaaaguccccuugauuuugauuuuc
agugugaauacaaaccaugaaaguguggccuaucgauccuuuagguccuc
ggaauuugaggcuagaggugccagaaaaguuaccacagggauaacuggcu
uguggcagucaagcguucauagcgacauugcuuuuugauucuucgauguc
ggcucuuccuaucauaccgaagcagaauucgguaagcguuggauuguuca
cccacuaauagggaacgugagcuggguuuagaccgucgugagacagguua
guuuuacccuacugaugaauguuaucgcaauaguaauugaacuuaguacg
agaggaacaguucauucggauaauugguuuuugcggcugucugaucaggc
auugccgcgaagcuaccauccgcuggauuauggcugaacgccucuaaguc
agaauccaugcuagaacgcgaugauuucuuugccuugcacaauauagaag
gauaagaauaaggcgucuuuuuggcgucgcugaaccauagcaggcuggca
acggugcucuuagcggaaaggcuuugggugcuugccggcgaauugcaaug
ucauuuugcgcaaggauaaaucauuuguauacgacuuaaauguacaacag
gguauuguaagcaguagaguagccuuguuguuacgaucugcugagauuaa
gccuucguugucugauuugu

........................<<<<<<.....<......>......>
>>>>.>...<.....<<....>>..<<<<<......<<.....>>.....
>>>>>..<<<..<........<<.<<<<<<...>>>>>>.>>........
>....<<<<<<<.<<<<.<<<<<<<<<<<<<<<<....<<<<......>>
>>.(.......<<<......)>>>..>.>>>>>>>>..>>>>>>>.....
>>>>>>>>>>>........<<<<<<<........>>>>>>>.....<<<<
<..((((((>>>>>..>>>....>..........................
...<<....>>...............<<<<....>>>>............
......................<<<<<<<<<<<<.......<<<<<<<.<
<<<<<<<<<<<<<<.....<<<<.<<<<......<<<<<<<....>>>>>
>>.......>>>>.>>.>.>.>>>>>.>>>>>>>>>>........<<<..
..>>>.....>>>>>>>....>>>>>>>>>>>>..<<<<........>>>
><<<<<<....<<<<<<.<<<<<<..<<<..<<<<<.....>>>>>..>>
>...<<....>>.......<<<<<..<<<<....>>>>...>>>>>.<<<
<<<<<...<<....>>...>>>.>>>.>>....>>>>>>.>>>>>>..<<
.<..<<<<<<<.....<<<<<<<<<..<<<....<<<<<<<....>>>>>
>>.....>>>...<<<<.<<.<<........>>>>>>>>....>>>>>>>
>>...<<.......>>......>>>>>>...>..>.>>..<<<<<<....
<.<<<....>>>.><<<<<<<<<<...<<<.<<.<<<<.....<<<....
<<<..<.<<<<..<<<<<<<.....>>>>>>>>>>>.>..>>>..>.>>.
....>>>>>...<<<<.<<<<.<......>>>>>.>>>>......>>>>.
>>>>>>>>.>>..<<<<.<<<<<.....>>>>>.>>>>....<<<.....
.>>>...<<<<<<<..<<<<(((.....<<<<<<......<.........
.<<<.<<<<<<<<<<.......<<<<<.<<<...<<.........>>...
>>>.........<<......>>...>>>>>...>>>>>>>>>>>>>....
......))).>....>>>>>>.>>>>..>>>>>>><<<<<<<<<......
...>>>>>>>>>....>>>>>>..<<<<<<<..<<......<<<<.<<<<
....>>>>>>>>...>>.>>>>>>>........>>>>>><<.....<<..
...<<<<<<.......>>>>>><<<<.....<<<<<.<<...<<<<<<..
.<<.....>>.>>>>>>....((<<<<<<...<<....<<<....>>>..
......<<<<<<......>>>>>>....>>....>>...<<<<<......
..>>>>>))<<<<.<<<<<<<...<.<<<....>>>.......<<<<<<.
..<<<<.<<<<.<<<<<<<<<<<<<..[[[..>>>>>><...<<<<<...
.<<<<<.....<<<.....>>>.....>>>>>.....>>>>>....><<<
<<<<......>>>>>>>>>>>.>.>>.>>>>.>>>>.........>>>>>
>.....>....>>>..>>>>>>>>>>>>..>>.....<<......>>>>>
>>.<<<<.....>>>>..>>>>..<<<<.....<<<<<<<.<<.......
........<<<<<...............>>>>><<.<<<<<<<<<<....
......<<<..<<<<....<<<<..>>>>....>>>>..>>>........
...................>>>>..>>>>>>.>>............<<<<
<<<<<..<<<.........>>>..<<<<<<<....<<<<.....>>>>..
....>>>>>>>.<.<<..<<<..<<<<<<....<<<....<<<<<....>
>>>.>...>>>.>>>>..>>>>>.<<<<<.......>>>>>.........
<...........<<<<<.......>>>>>.....>.....>>.>..>>.>
>>>>>>.....>>.>>>>>>>..>>>>>>....>>....<<<<<......
..>>>>>....<<<<<..<<<.<<......<<<<.<<<<<<..<<<<<.<
<<<<<<<....<<<<.<.....<<<<..........<<<<<..<<....>
>............>>>>>.>>>>.....>>>.>>......<<<<<.....
<<<<....<<<......>>>.................<<<<<.....>>>
>>..>>>>..>>>>>.....>>>>>>>>..>>>>>...<<<.....>>>.
.<<<<<<...........>>>>>>..<<...<<<<<<<...<<<<<<...
....>>>>>>.....<<[..(((>>....>>>>>>>...<<<.<<<<<..
.....>>>>.>>>>.<<<....>>>.>>))).]....<<<<<<<..<<<<
.))))))>>>>>>>>>>>.........>>>>..>>>>>>.......<<.<
...<<<<<<...<<.......>>...>>>>>>....>.>.>.......<<
<<<..<<<<<..<.<<<<<<<......>>>>>>>...>.<<<........
.>>>.....>>>>>......>>>.>>....<<<<<<<....>>>.>>>>.
...>>..>>>.>>>>>....<<<<<<<<..<<<<...<<<<<<.......
........>>>>>>..<<<<<...<<<<....<<<<<<<<<<]]].>>>>
>..>>>>>...>>>>..>>>>>.<<<<...<<<<<...........>>>>
>...>>>>.>>>>....>>>>>>>>.......<<<<<<.<<<.<<....<
<..........<<<<<<.....>>>>>>......>>.......<<<<<<.
..<<<<<<<..<<<......>>>..>>>>>>>.>>>>>>...........
..>>.>>>.>>>>>>..<<<<<.<.............>.....<<<<<<<
<<.<<<....<<<<.<..<<<<<......>>>>>..>>>>>...>>>...
.>>>>.>>>>>..>>>>>..

Receptor-Ligand Complex Structure

Structure summary

• PDB: 5it7 Structural characterization of ribosome recruitment and translocation by type IV IRES.
• Resolution: 3.6 Å
• Binding residue (original residue number in PDB): V2 N3 P5 R8 K9 T10 Y11 K13 R18 K19 H20 Y28 G31 K32 A33 S34 Y36 A37 Q38 G39 R41 R42 R45 K46 G49 F50 G52 Q53 T54 Q56 I57 F58 K60 K61 A62 K63 T65 K67 V69 T79 K80 Q82 K86 R87 C88 K97 K98
• Binding residue (residue number reindexed from 1): V1 N2 P4 R7 K8 T9 Y10 K12 R17 K18 H19 Y27 G30 K31 A32 S33 Y35 A36 Q37 G38 R40 R41 R44 K45 G48 F49 G51 Q52 T53 Q55 I56 F57 K59 K60 A61 K62 T64 K66 V68 T78 K79 Q81 K85 R86 C87 K96 K97

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0006412 translation
• GO:0046677 response to antibiotic
• GO:0046898 response to cycloheximide

Cellular Component

• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:5it7, PDBe:5it7, PDBj:5it7
• PDBsum: 5it7
• PubMed: 27159451
• UniProt: P31027|RL44_KLULA Large ribosomal subunit protein eL42 (Gene Name=RPL44)