Structure of PDB 8c3a Chain o Binding Site BS01

Receptor Information

>8c3a Chain o (length=234) Species: 5476 (Candida albicans)

ETLQKKEKAQQKTAEERAAAKKVRKAANKEKRKVIFDRAAAYQKEYTEAE
RSVIKAKRDAKASNSYYVDAQPKLVFVVRIKGINKIPPKPRKVLQLLRLT
QINAGVFVRLTKATSELIKLAEPYVAYGYPSLSTIRQLVYKRGFGKVNKQ
RIALSDNAIIEANLGKFNILSIEDLIHEIYTVGPNFKQVSNFLWPFKLSN
PNGGFRARKFQHFIQGGDTGNREQFINALVKQMN

Ligand information

>8c3a Chain 1 (length=3217)

ugaccucaaaucagguaggacuacccgcugaacuuaagcauaucaauaag
cggaggaaaagaaaccaacagggauugccucaguagcggcgagugaagcg
gcaaaagcucaaauuugaaaucuggcgucuuuggcguccgaguuguaauu
ugaagaagguaucuuugggcccggcucuugucuauguuccuuggaacagg
acgucacagagggugagaaucccgugcgaugagaugacccgggucugugu
aaaguuccuucgacgagucgaguuguuugggaaugcagcucuaagugggu
gguaaauuccaucuaaagcuaaauauuggcgagagaccgauagcgaacaa
guacagugauggaaagaugaaaagaacuuugaaaagagagugaaaaagua
cgugaaauuguugaaagggaagggcuugagaucagacuugguauuuugca
ugcgguuuaccgggccagcaucgguuuggagcggcaggauaauggcggag
gaauguggcacggcuucugcuguguguuauagccucugacgauacugcca
gccuagaccgaggacugcgguuuuuaccuaggauguuggcauaaugaucu
uaagucgcccgucuugaaacacggaccaaggagucuaacgucuaugcgag
uguuuggguguaaaacccguacgcguaaugaaagugaacgaagguggggg
cccauuagggugcaccaucgaccgauccugauguguucggauggauuuga
guaagagcauagcuguugggacccgaaagauggugaacuaugccugaaua
gggugaagccagaggaaacucugguggaggcucguagcgguucugacgug
caaaucgaucgucgaauuuggguauaggggcgaaagacuaaucgaaccau
cuaguagcugguuccugccgaaguuucccucaggauagcagaagcucgua
ucaguuuuaugagguaaagcgaaugauuagaagucuugggguugaaauga
ccuuaacuuauucucaaacuuuaaauauguaagaaguccuuguugcuuaa
uugaacguggacaauugaaugaagagcuuuuagugggccauuuuugguaa
gcagaacuggcgaugcgggaugaaccgaacgugaaguuaaagugccggaa
ugcacgcucaucagacaccacaaaagguguuaguucaucuagacagccgg
acgguggccauggaagucggaauccgcuaaggaguguguaacaacucacc
ggccgaaugaacuagcccugaaaauggauggcgcucaagcgugcuacuua
uacuucaccgugauugcuguuuugacgcuuucacgaguaggcaggcgugg
aggucagugacgaagccuuugcuguaaagcugggucgaacggccucuagu
gcagaucuuggugguaguagcaaauauucaaaugagaacuuugaagacug
aaguggggaaagguuccaugucaacagcaguuggacauggguuagucgau
ccuaagagauggggaagcuccguuucaacgugcuugauuuuucaggccaa
ccaucgaaagggaauccgguuaaaauuccggaacuuggauauggauucuu
cacggcaacguaacugaauguggagacgucggcgugagcccugggaggag
uuaucuuuucuucuuaacagcuuaucacccuggaauugguuuauccggag
auggggucuuauggcuggaagagcgcgguaauuuugccgcguccggugcg
cuuacgacgguccuugaaaauccacaggaaggaauaguuuucaugccaag
ucguacucauaaccgcagcaggucuccaagguuaacagccucuaguugau
agaauaauguagauaagggaagucggcaaaauagauccguaacuucggga
uaaggauuggcucuaaggaucgguuuaacgaucaacuuagaacugguacg
gacaaggggaaucugacugucuaauuaaaacauagcauugugauggucag
aaagugauguugacacaaugugauuucugcccagugcucugaaugucaaa
gugaagaaauucaaccaagcgcggguaaacggcgggaguaacuaugacuc
ucuuaagguagccaaaugccucgucaucuaauuagugacgcgcaugaaug
gauuaacgagauucccacugucccuaucuacuaucuagcgaaaccacagc
caagggaacgggcuuggcagaaucagcggggaaagaagacccuguugagc
uugacucuaguuugacauugugaaaagacauggaggguguagaauaagug
ggagcuucggcgccggugaaauaccacuaccucuauaguuuuuuuacuua
uucaaugaagcggagcuggaggucaaacuccacguucuagcauuaagccc
ucugggcgauccggguugaagacauugucagguggggaguuuggcugggg
cggcacaucuguuaaacgauaacgcagguguccuaagggggacucaugga
gaacagaaaucuccaguagaacaaaaggguaaaaguccccuugauuuuga
uuuucagugugaauacaaaccaugaaaguguggccuaucgauccuuuagu
cccucggaauuugaggcuagaggugccagaaaaguuaccacagggauaac
uggcuuguggcagucaagcguucauagcgacauugcuuuuugauucuucg
augucggcucuuccuaucauaccgaagcagaauucgguaagcguuggauu
guucacccacuaauagggaacgugagcuggguuuagaccgucgugagaca
gguuaguuuuacccuacugaugaauguuaucgcaauaguaauugaacuua
guacgagaggaaccguucauucagauaauugguuuuugcggcugucugau
caggcaacgccgcgaagcuaccaucugcuggauuauggcugaacgccucu
aagucagaauccaugcuagaacgcgaugauuuugcccugcacauuuuaga
uggauacgaauaagacuuuuuagucgcuggaccauagcaggcuggcaacg
gugcgcuuagcggaaaggcuuugugcgcuugccggcggauagcaauguca
acaugcgcggggauaaauccuuugcauacgacuuagauguacaacggagu
auuguaagcaguagaguagccuuguuguuacgaucugcugagauuaagcu
cuuguugucugauuugu

.......................<<<<<<.....<......>......>>
>>>.>...{.....<<....>>..<<<<<......<<.....>>.....>
>>>>..[[[...........<<.<<<<<<...>>>>>>.>>.........
....<<<<<...<<<<<<<<<<<<.<<<<<<<<....<<<<......>>>
>.(.......<<<......)>>>..>.>>>>>>>....>>>>>>>>....
>>>>..>>>>>........<<<<<<<........>>>>>>>.....((((
(..<<<<<<)))))..]]]....}..........................
...<<....>>...............<<<<....>>>>............
......................<<<<<<<<<<<<.....<<<<..<<<<.
.>>>>..>>>>..<<<<<<<.<<<<<<<<.<<<<<<<....<<.<<<<<<
<......<<<<<<......>>>>>>.......>>>>>>.>.>>.>>>>>.
>>>>>>>>>>........<<<....>>>.....>>>>>>>....>>>>>>
>>>>>>..<<<<........>>>><<<<<<....<<<<<<.<<<<<<..<
<<..<<<<<.....>>>>>..>>>...<<....>>.......<<<<<..<
<<<....>>>>...>>>>>.<<<<<<<<...<<....>>...>>>.>>>.
>>....>>>>>>.>>>>>>..<<.<..<<<<<<<.....<<<<<<<<<..
<<<....<<<<<<<....>>>>>>>.....>>>...<<<..<<.<<....
....>>>>.>>>....>>>>>>>>>...<<.......>>......>>>>>
>...>..>.>>..<<<<<<....<.<<<....>>>.><<<<<<<<<<...
<<<.<<<<<<<.....<<<....<<<....<<<<...<<<<<.......>
>>>>.>>>>....>>>..>.>>.....>>>>>...<<<<.<<<<.<....
..>>>>>.>>>>.....>>>>>.>>>>>>>>.>>..<<<<.<<<<<....
.>>>>>.>>>>....<<<......>>>...<<<<<<<..<<<<(((....
.<<<<<<.....<<..........<<<.<<<<<<<<<<.......<<<<<
.<<<...<<.........>>...>>>.........<<......>>...>>
>>>...>>>>>>>>>>>>>..........))).>>...>>>>>>.>>>>.
.>>>>>>><<<<<..<<.........>>..>>>>>....>>>>>>..<<<
<<<<<.<<......<<<<..<<<....>>>.>>>>...>>>>>>>>>>..
......>>>>>><<.....<<.....<<<<<<.......>>>>>><<<<.
....<<<<<.<<...<<<<<<...<<.....>>.>>>>>>....((<<<<
<<...<<....<<<....>>>..........<<<<<<....>>>>>>...
...>>....>>...<<<<<........>>>>>))<<<<.<<<<<<<....
.<<<....>>>.......<<<<<<...<<<<.<<<<<<<.<<<<<<<<<<
..{{{..>>>>>><...<<<<<....<<<<<<....<<<.....>>>...
.>>>>>>.....>>>>>....><<<<<<<......>>>>>>>>>>>...>
>>>>>>.>>>>.........>>>>>>..........>>>..>>>>>>>>>
>>>..>>.....<<......>>>>>>>.<<<<.....>>>>..>>>>..<
<<<.....<<<<<<<.<<...............<<<<<............
...>>>>><<.<<<<<<<<<<<......>>>>>..>>>>>>.>>......
......<<<<<<<<<..<<<.........>>>..<<<<<<<....<<<<.
....>>>>......>>>>>>>...<<..<<<..<<<<<<....<<<....
.<<<<....>>>>.....>>>.>>>>..>>>>><<<<<<.......>>>>
>>........<...........<<<<<.......>>>>>.....>.....
>>....>>.>>>>>>>.....>>.>>>>>>>..>>>>>>....>>....<
<<<<........>>>>>....[[[[[..{{{.{{......[[[[.{{[[[
[..<<<<<.<<<<<<<<..<<<<<<<<<<...<.<<..............
..............................>>.>...>>>>>>>>>>...
<<<<<.....<<<<..<<<<<......>>>>>..............<<<<
...>>>>..>>>>..>>>>>.....>>>>>>>>..>>>>>...<<<....
.>>>..<<<<<<...........>>>>>>..<<...<<<<<<<...<<<<
<<.......>>>>>>.....<<...(((>>....>>>>>>>...<<<.<<
<<<.......>>>>.>>>>.<<<....>>>.>>))).......((((((.
.((((.>>>>>>))))))))))..........]]]]..}}]]]]......
.<<.<...<<<<<<...<<.......>>...>>>>>>....>.>.>....
...<<<<<.<<<<<<..<.<<<<<<<......>>>>>>>...>.<<<...
......>>>.....>>>>>..>...>>>.>>....<<<<<<<....>>>.
>>>>....}}..}}}.]]]]]....<<<<<<<<..<<<<...<<<<<...
..............>>>>>..<<<<<...<<<<....<<<<<<<<<<}}}
.>>>>>..>>>>>...>>>>..>>>>>.<<<<...<<<<<..........
.>>>>>...>>>>.>>>>....>>>>>>>>....<<<<<<.<<<.<....
.<<..........<<<......>>>......>>.......<<<<<<...<
<<<<<<..<<<......>>>..>>>>>>>.>>>>>>..............
>.>>>.>>>>>>..<<<<<<<.............>>....<<<<<<<<<.
<<<....<<<<.<..<<<<<......>>>>>..>>>>>...>>>....>>
>>.>>>>>..>>>>>..

Receptor-Ligand Complex Structure

Structure summary

• PDB: 8c3a New crystal system to promote screening for new eukaryotic inhibitors
• Resolution: 3.0 Å
• Binding residue (original residue number in PDB): Q17 R24 K29 R31 K32 N35 R39 R58 R65 K68 K88 G89 I90 N91 K92 P95 K96 K99 L103 R105 Q108 I109 N110 A120 E123 S140 R143 K148 R149 K156 Q157 R158 D163 K194 N198 K204 L205 S206 N207 P208 N209 G210 G211 F212 R215 K216 F217 K238
• Binding residue (residue number reindexed from 1): Q10 R17 K22 R24 K25 N28 R32 R51 R58 K61 K81 G82 I83 N84 K85 P88 K89 K92 L96 R98 Q101 I102 N103 A113 E116 S133 R136 K141 R142 K149 Q150 R151 D156 K187 N191 K197 L198 S199 N200 P201 N202 G203 G204 F205 R208 K209 F210 K231

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0000463 maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)

Cellular Component

• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit

External links

• PDB: RCSB:8c3a, PDBe:8c3a, PDBj:8c3a
• PDBsum: 8c3a
• UniProt: A0A8H6BVN2