Structure of PDB 7ase Chain o Binding Site BS01

Receptor Information

>7ase Chain o (length=190) Species: 5693 (Trypanosoma cruzi)

MSAKAPKLFNKWSYENLQTTEIALSDHISKTATYVPHSAGRWQKKRFRKA
RIPIVERLTNGLMFKGRGNGKKLQAVRLVKHTLEIIHLLTDQNPLQVVID
AVSKGAPREDSTRVGSGGVVRRQAVDVSPMRRVNEAIYLMCKGAREAAFR
NLKTLPECLADEIVNASKGSSNSYAIKKKDEVERVAKANR

Ligand information

>7ase Chain 0 (length=2150)

gaucugguugauucugccaguagucauaugcuuguuucaaggacuuagcc
augcaugccucagaaucacugcauugcaggaaucugcgcauggcucauua
caucagacguaaucugccgcaaaaaucuugcggucuccgcaacauuggau
aacuuggcgaaacgccaagcuaauacaugaaccaaccggauguucucugu
uccggcggcagggcaaccugcugccaugggacguccagcgaaugaaugaa
aguaaaaccaaugccuucaccggcaguaacacucagaaguguugauucaa
uucauuccgugcgaaagccggguuuuuuuauccggcgucuuuugacgaac
aacugcccuaucagccagcgauggccguguaguggacugccauggcguug
acgggagcgggggauuaggguucgauuccggagagggagccugagaaaua
gcuaccacuucuacggagggcagcaggcgcgcaaauugcccaaugucaaa
aaaaaaagaugaggcagcgaaaagaaauagagccgacagugcuuuugcau
ugucguuuucaaugggggauauuuaaacccauccaaaaucgaguaacaau
uggaggacaagucuggugccagcacccgcgguaauuccagcuccaaaagc
guauauuaaugcuguugcuguuaaaggguucguaguugaauugagggccu
cuaaggcgcaaugguuuagucccauccacuucggauuggugacccaugcc
cuugugguccgugaacagacauucagaaacaaaaaacacgggagugguac
cuuuccugauuaucgcaugucaugcaugccagagggcgcccgugauuuuu
uacugugacuaaaaaagugugaccaaagcagucauucgacuugaauuaga
aagcaugggauaacaaaggagcagccucugggccaccguuucggcuuuug
uugguuuuaaaaguccauuggagauuauggggcagugugacaagcggcug
ggugcacucgucgccuuugcaacucacggcauccaggaaugaaggagggu
aguucgggggagaacguacuggugcgucagaggugaaauucuuagaccgc
accaagacgaacuacagcgaaggcauucuucaaggauaccuuccucaauc
aagaaccaaaguguggggaucgaagaugauuagagaccauuguaguccac
acugcaaacgaugacacccaugaauuggggaguuuuuggucguaggcgug
gucgggcuugauuauuauuuuucaucccguuccucgucucgccaaugaau
auuaaauuuacgugcauauucuuuuuggucuucguuuuuuuacggcgagg
gccuuuaacgggaauauccucagcacguuaucugacuucuucacgcgaaa
gcuuugagguuacagucucaggggggaguacguucgcaagagugaaacuu
aaagaaauugacggaauggcaccacaagacguggagcgugcgguuuaauu
ugacucaacacggggaacuuuaccagauccggacagggugaggauugaca
gauugaguguucuuucucgauccccugaaugguggugcauggccgcuuuu
ggucgguggagugauuuguuugguugauuccgucaacggacgagauccaa
gcugcccaguaggauucagaauugcccccuuccgcggguuuuacccaagg
gggggcgguauucgcuuguauccuucucugcgggauuccuuguuuugcgc
aaggugagauuuugggcaacagcaggucugugaugcuccucaauguucug
ggcgacacgcgcacuacaaugucagugagaacaagaaaaacgacucuugu
cggaccuacuugaucaaaagagugggaaaaccccggaaucacguagaccc
acuugggaccgaguauugcaauuauuggucgcgcaacgaggaaugucucg
uaggcgcagcucaucaaacugugccgauuacgucccugccauuuguacac
accgcccgucguuguuuccgaugauggugcaauacaggugaucggacagu
cgagugcuucacuugaccgaaaguucaccgauauuucuucaauagaggaa
gcaaaagucguaacaagguagcuguaggugaaccugcagcuggaucauuu

...<<<<<...{{{{>>>>>{{{{.[[[[[[...[[[..........[[[
.[[[..<<....<<....<<<<...>>>>...>>.>>......{{.....
...[[[..[[..[[...[[.[[.....<<<<<<...>>>>>>...<<...
..<<<<<<.....>>>>>>......>>..........<<<<<.<<<....
...<<<<<<<<<....>>>>>>>>>...>>>>>>>>.[[((((((((...
...<<<<<<<<<<<<......>>>.........................)
)))))))...]]....<<<<<<........>>>>>>...]].]]..]]]]
..]]].<<<....<<<....<<<<<.<<.......>>.>>>>>>>>....
..>>>..<<<<<<<<....>>>>...>>>>.}}.<<.<<<..........
>>>.>>.<<<<....>>>>...]]]]]].........<<<....<<<...
.......>>>..>>>.......]]]....<<<.<<<<<<<<<....>>>>
>>>>>..>>>.....<<<<............>>>.....>.........<
<<<<((....<.<<<<.....<<..))>>.......>>>>>>>>>>..]]
]]]]}}}}.........{{{{{.....[[[[...[[[.......<<<<<.
<.<<<<.<<<..<<............................>>..>>>>
>>>>.>>>>>..........................<<<<<<..<....<
<<<.<.........<<<<.......>>>>..>>>>>.>>>>>>>.....<
<<<<<<<.......<<...<.......>..<<<....>>>.....>>...
...>>>>>..>>>...........[[..(((((..<<<...>>>.((..>
>>>>>>>>))..)))))..]].......<<<<<...<<<....<<<<<..
<<<..>>>.>>>>>..<<<>>>..>>>.>>.>>>............<<<<
<..<<...<<<<<<<<..<<<<<<<<...<<<......>>>......>>>
>>>>.>.>>.......<<....>>.>>>>>>..>>.>>>.>>....]]].
..]]]]...<<<<<<<<...<...<<<<.<.....>.>>>>...>>>>>>
>>>.........<<<.<<...<..<<<<<.......<<<.<<.<<<<<.<
<..<<<...<............>..>>>...>>.>>>>>>>>>>......
.....................<.<<...<<<<<<<.........>>>>>>
>.........>>.>................>>>>>..>......<<..}}
>>.....>>.....>>>.}}}.<<<......<<<<....>>>>....>>>
..}}}}..<<<<<.<<<<<<<..<<.<<<<<<.....<<<<<<.......
<<........>>.......<..<<.<<......<.<<<........<<.<
<<........>>>.>>......>>>.>.<.<<.<<<..<<.<<<<<<...
.<<<.<<<<......>>...<<<......>>>...>>.>>>....<<<<<
.<<<<.....<<<<<.<<..<.....<<<<<<<..<<<<...>>>>..>>
>>>>>......>....>>.>>>>>.....>>>>...<<.<.<<.....>>
>.>>.......>>>>>....>>>.>>>.....>>>>>>>>......>.>.
>>.>...>.>>>>>....................................
....<<<<<<<........>>>>>>>........................
....<....<<<.<<..........>>.>>>.....>.....>>>>>>..
..<<<<<<<.........>>>>>>>......>>...>>>>>>>>>>.>>.
...<..<<.<.<<<<<<<<..<<.<<<.<.<<<<..<<<<<.<...<.<<
<<<<<<...>>>>>>>>.>...>.>>>>>..>>>>.>.>>>.>>..>>>>
>>>>..>.>>...>.....<<<<<<<<<....>>>>>>>>>.........

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7ase Structural Differences in Translation Initiation between Pathogenic Trypanosomatids and Their Mammalian Hosts.
• Resolution: 3.33 Å
• Binding residue (original residue number in PDB): H37 Q43 K45 R46 N60 L62 F64 K65 R67 G68 G70 K72 Q74 R77 R131 N134 Y138 R145 F149 R150 K153 L155
• Binding residue (residue number reindexed from 1): H37 Q43 K45 R46 N60 L62 F64 K65 R67 G68 G70 K72 Q74 R77 R131 N134 Y138 R145 F149 R150 K153 L155

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003729 mRNA binding
• GO:0003735 structural constituent of ribosome
• GO:0019843 rRNA binding

Biological Process

• GO:0000028 ribosomal small subunit assembly
• GO:0006412 translation

Cellular Component

• GO:0005840 ribosome
• GO:0015935 small ribosomal subunit
• GO:0022627 cytosolic small ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:7ase, PDBe:7ase, PDBj:7ase
• PDBsum: 7ase
• PubMed: 33357443
• UniProt: Q4E0Q3