Structure of PDB 6tnn Chain o Binding Site BS01

Receptor Information

>6tnn Chain o (length=82) Species: 224308 (Bacillus subtilis subsp. subtilis str. 168)

SKKGVGSTKNGRDSEAKRLGAKRADGQFVTGGSILYRQRGTKIYPGENVG
RGGDDTLFAKIDGTVKFERFGRDRKKVSVYPV

Ligand information

>6tnn Chain U (length=2930)

acgguuaaguuagaaagggcgcacgguggaugccuuggcacuaggagccg
augaaggacgggacgaacaccgauaugcuucggggagcuguaagcaagcu
uugauccggagauuuccgaauggggaaacccaccacucguaauggagugg
uauccauaucugaauucauaggauaugagaaggcagacccggggaacuga
aacaucuaaguacccggaggaagagaaagcaaaugcgauucccugaguag
cggcgagcgaaacgggaucagcccaaaccaagaggcuugccucuuggggu
uguaggacacucuguacggaguuacaaaggaacgagguagaugaagaggu
cuggaaaggcccgccauaggagguaacagcccuguagucaaaacuucguu
cucuccugaguggauccugaguacggcggaacacgugaaauuccgucgga
auccgggaggaccaucucccaaggcuaaauacucccuagugaccgauagu
gaaccaguaccgugagggaaaggugaaaagcaccccggaaggggagugaa
agagauccugaaaccgugugccuacaaguagucagagcccguuaacgggu
gauggcgugccuuuuguagaaugaaccggcgaguuacgaucccgugcaag
guuaagcagaagaugcggagccgcagcgaaagcgagucugaauagggcgc
augaguacguggucguagacccgaaaccaggugaucuacccauguccagg
gugaaguucagguaacacugaauggaggcccgaacccacgcacguugaaa
agugcggggaugagguguggguaggggugaaaugccaaucgaaccuggag
auagcugguucucuccgaaauagcuuuagggcuagccucaagguaagagu
cuuggagguagagcacugauuggacuaggggccccuaccggguuaccgaa
uucagucaaacuccgaaugccaaugacuuauccuugggagucagacugcg
agugauaagauccguagucgaaagggaaacagcccagaccgccagcuaag
gucccaaaguauacguuaaguggaaaaggauguggaguugcuuagacaac
caggauguuggcuuagaagcagccaccauuuaaagagugcguaauagcuc
acuggucgagugacucugcgccgaaaauguaccggggcuaaacguaucac
cgaagcugcggacuguucuucgaacagugguaggagagcguucuaagggc
ugugaagccagaccggaaggacugguggagcgcuuagaagugagaaugcc
gguaugaguagcgaaagaggggugagaauccccuccaccgaaugccuaag
guuuccugaggaaggcucguccgcucaggguuagucgggaccuaagccga
ggccgaaaggcguaggcgauggacaacagguugauauuccuguaccaccu
ccucaccauuugagcaauggggggacgcaggaggauaggguaagcgcggu
auuggauauccgcguccaagcaguuaggcugggaaauaggcaaauccguu
ucccauaaggcugagcugugauggcgagcgaaauauaguagcgaaguucc
ugauuccacacugccaagaaaagccucuagcgaggugagaggugcccgua
ccgcaaaccgacacagguaggcgaggagagaauccuaaggugaucgagag
aacucucguuaaggaacucggcaaaaugaccccguaacuucgggagaagg
ggugcucuguuagggugcaagcccgagagagccgcagugaauaggcccag
gcgacuguuuagcaaaaacacaggucucugcgaagccguaaggcgaagua
uaggggcugacgccugcccggugcuggaagguuaagaggagcgcuuagcg
uaagcgaaggugcgaauugaagccccaguaaacggcggccguaacuauaa
cgguccuaagguagcgaaauuccuugucggguaaguuccgacccgcacga
aaggcgcaacgaucugggcacugucucaacgagagacucggugaaauuau
aguaccugugaagaugcagguuacccgcgacaggacggaaagaccccgug
gagcuuuacugcagccugauauugaauguugguacagcuuguacaggaua
gguaggagccuuggaaaccggagcgccagcuuugguggaggcaucggugg
gauacuacccuggcuguauugaccuucuaacccgccgcccuuaucgggcg
gggagacagugucaggugggcaguuugacuggggcggucgccuccuaaaa
gguaacggaggcgcccaaagguucccucagaaugguuggaaaucauucgc
agaguguaaaggcacaagggagcuugacugcgagaccuacaagucgagca
gggacgaaagucgggcuuagugauccggugguuccgcauggaagggccau
cgcucaacggauaaaagcuaccccggggauaacaggcuuaucucccccaa
gaguccacaucgacggggagguuuggcaccucgaugucggcucaucgcau
ccuggggcuguagucggucccaaggguugggcuguucgcccauuaaagcg
guacgcgagcuggguucagaacgucgugagacaguucggucccuauccgu
cgcgggcgcaggaaauuugagaggagcuguccuuaguacgagaggaccgg
gauggacgcaccgcugguguaccaguuguucugccaagggcaucgcuggg
uagcuaugugcggacgggauaagugcugaaagcaucuaagcaugaagccc
cccucaagaugagauuucccauuccgcaaggaaguaagaucccugaaaga
ugaucagguugauaggucugagguggaaguguggcgacacauggagcuga
cagauacuaaucgaucgaggacuuaaccau

..<<<<<<<<......<<<<<<<<<<<....{[[..[[<<<<<<<[[<<.
.....<<<<<<.....((.>>>.....<<<<<<<..<<.))......>>.
....>>>>>>>...>>>....<<<....>>><<<<<<<......>>>>>>
>.<<<<<<<<<.........>>>>>>>.>>.>>....<<<<<<.....<<
....>>......>>>>>.>.........<<....>>...<<<<......<
<.....>>.....>>>>...(((....<<<<<<<<....>>>>>>>>...
...<<<<<<<<<.....<<<<.......<<<<<<<<<<.<<<.....<<<
<......>>>>.((......<<<.....))>>>...>>>...>>>>>>>>
>>>>>>.>>>>>..>>>>.....<<<<<<<<.........>>>>>>>>..
....<<<<<..<<<<>>>>>..)))......]].>>>>>>>.]]......
.........<<....>>....]].........<<<<....>>>>......
.......}....>>>>>>>>>>>.......<<<<..<<<<......>>>>
..>>>>...<.<........>.>.<<<<<<....<<<<<<<.<<<<<..<
<<...<<<.....>>>...>>>...<<....>>..<<<<.....>>>>..
....>>>>>.>>>>>>>..<<.<..<<<<<<<.....<<<<<<<<<..<<
<....<<<<<<......>>>>>>.....>>>...<<<.<<<<<.......
.>>>>>>>>....>>>>>>>>>...<<<.....>>>......>>>>>>..
.>..>.>>..<<<<<<....<<<<<....>>>>><<<<<<<<<...<<<<
<<<<<.....<<<...<<<<<<<<......<<<<.....>>>>.......
>>>>>>>>..>>.>.....>>>>.>>>>>..>>>>>>>.>>..<<<<<<<
.<<......>>.>>>>>>>....<<<......>>>...<<<<.<<<...<
<<(((.....<<<<<<...........<<..<<<<<<<<<........<<
<<<.<<.....................>>.........<<......>>..
.>>>>>....>>>>>>>>>.>>...........))).....>>>>>>.>>
>...>>>>>>><<<<<<<...>>>>>>>....>>>>>><.<<<<<<<.<<
......<<<<<.<<....>>.>>>>>...>>.>>>>>>>..>.....>>>
>>><......<<....<<<<<<.......>>>>>><<<<....<<<<<.<
<<..<<<<<<...<<.....>>.>>>>>>....((<<<<.<<...<<<..
.<<<....>>>...>>>...>>...<<<<<........>>>>>))<<<<<
.<<<<<<......<<...<<<<....<<<<..<<<..<<<.<.<<<.<..
...{....>.>>>.<<..<......<<.<.<<<<<<<.<<.....>>>>>
>>>>.....>>>......>...>><..<<...........>>...>>.>>
>...>>>...>>>>.........>>>>..>>..>>>>>>>>>>>>>>>.>
>>.....<<......>>>>>>>.<<<<.....>>>>..>>>>..<<<<..
..<<<<<<<<.<<<........<.....<<<<<....<<..>>.....>>
>>><<<<<....<<<<....>>>>...>>>>>............<<<<<<
<<<..<<<.........>>>..<<<<<<<.....<<<....>>>......
.>>>>>>>..<<<<.<<<..<<<<<<<..<<<...<<...<<<<<..<<.
...>>...>>>>>...>>...>>>>>>>>..>>>>><<<<<<.......>
>>>>>........<...........<<<<<.......>>>>>.....>..
..>>>>...>>.>>>>>>>..>.>>>.>>>>>>>>.>>>>>>.....>..
...<<<<<........>>>>>..<<<<<<<<.(((.[[......<<<<.[
[<<<<..<<<<<.<<<<<<<<<.<<<.<<<..<<<<<<............
<<<<<..<<...............................>>........
....>>>>>..>.>>>>>..>>>.>>>....<<.<<<<<.......>>>>
>>>.....>>>>>>>>>..>>>>>..<<<<.....>>>><<<<<<.....
......>>>>>>..<<...<<<<<<<...<<<<<<.......>>>>>>..
...<<.[.(((>>....>>>>>>>...<<<<..<<<......>>>..>>>
>.<<<....>>>.>>)))]......<<<<<<<.((..>>>>..))>>>>>
>>.............>>>>..]]>>>>.......<<<<...<<<<<<...
<<.......>>...>>>>>>....>>>.>.......<<<<<.<<<<<<..
<<<<<<<<<......>>>>>>..>>>.<<<<<.....>>>>>.....>>>
>>..>...>>>.>>....<<<<<<<....>>>.>>>>....]]..)))>>
>>>>>>....<<<<<<<...<<<<..<<<<<<<...............>>
>>>>>.<<<<<...<<<<...<<<<<<<<<<<.}..>>>>>>..>>>>>.
..>>>>.>>>>>...<<<....<<<<<...........>>>>>....>>>
.>>>>.......>>>>>>>..<<<<....>>>>....<<<<<<<<<....
...>>>>>....<<<<<<<<.<<<....<<<<<....>>>>>...>>>..
>>>>>.>>>...>>>>....>>>>>>>>..

Receptor-Ligand Complex Structure

Structure summary

• PDB: 6tnn Structures of B. subtilis Maturation RNases Captured on 50S Ribosome with Pre-rRNAs.
• Resolution: 3.07 Å
• Binding residue (original residue number in PDB): K12 G16 K19 N20 R22 D23 S24 E25 K27 R28 A34 D35 G41 G42 I44 Y46 R47 R49 G50 Y54 G62 G63 D64 F68 F77 R84 K85
• Binding residue (residue number reindexed from 1): K2 G6 K9 N10 R12 D13 S14 E15 K17 R18 A24 D25 G31 G32 I34 Y36 R37 R39 G40 Y44 G52 G53 D54 F58 F67 R74 K75

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0006412 translation

Cellular Component

• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:6tnn, PDBe:6tnn, PDBj:6tnn
• PDBsum: 6tnn
• PubMed: 32991829
• UniProt: P05657|RL27_BACSU Large ribosomal subunit protein bL27 (Gene Name=rpmA)