Structure of PDB 7o9m Chain n Binding Site BS01

Receptor Information

>7o9m Chain n (length=215) Species: 9606 (Homo sapiens)

KNRTGAEHLWLTRHLRDPFVKAAKVESYRCRSAFKLLEVNERHQILRPGL
RVLDCGAAPGAWSQVAVQKVNAAGTDPSSPVGFVLGVDLLHIFPLEGATF
LCPADVTDPRTSQRILEVLPGRRADVILSDMAPNATGFRDLDHDRLISLC
LTLLSVTPDILQPGGTFLCKTWAGSQSRRLQRRLTEEFQNVRIIKPEASR
KESSEVYFLATQYHG

Ligand information

>7o9m Chain A (length=1454)

gcuaaaccuagccccaaacccacuccaccuuacuaccaaaccuuagccaa
accauuuacccaaauaaaguauaggcgauagaaauugaaaccuggcgcaa
uagauauaguaccgcaagggaaagaugaaaaaaccaagcauaauauagca
aggacuaaccccuauaccuucugcauaaugaauuaacuagaaauaacuuu
gcaaggagagccaaagcuaagacccccgaaaccagacgagcuaccuaaga
acagcuaaaagagcacacccgucuauguagcaaaauagugggaagauuua
uagguagaggcgacaaaccuaccgagccuggugauagcugguuguccaag
auagaaucuuaguucaacuuuaaauuugcccacagaacccucuaaauccc
cuuguaaauuuaacuguuaguccaaagaggaacagcucuuuggacacuag
gaaaaaaccuuguagagagaguaaaaaauuuaacacccauaguaggccua
aaagcagccaccaauuaagaaagcguucaagcucaacacccacuaaaaaa
ucccaaacauauaacugaacuccucacacccaauuggaccaaucuaucac
ccuauagaagaacuaauguuaguauaaguaacaugaaaacauucuccucc
gcauaagccugcgucagauacacugaacugacaauuaacagcccaauauc
uacaaucaaccaacaagucauuauuacccucacugucaacccaacacagg
caugcucauaaggaaagguuaaaaaaaguaaaaggaacucggcaaaucuu
accccgccuguuuaccaaaaacaucaccucuagcaucaccaguauuagag
gcaccgccugcccagugaucacuuguuccuuaaauagggaccuguaugaa
uggcuccacgaggguucagcugucucuuacuuuuaaccagugaaauugac
cugcccgugaagaggcgggcauaacacagcaagacgagaagacccuaugg
agcuuuaauuuauuaaugcaaacaccugcauuaaaaauuucgguuggggc
gaccucggagcagaacccaaccuccgagcaguacaugcuaagacuucacc
agucaaagcgaacacucaauugauccaauaacuugaccaacggaacaagu
uacccuagggauaacagcgcaauccuauucuagaguccauaucaacaaua
ggguuuacgaccucgauguuggaucaggacaucccgauggugcagccgcu
auuaaagguucguuuguucaacgauuaaaguccuacgugaucugaguuca
gaccggaguaauccaggucgguuucuaucuacuuucaaauuccucccugu
acgaaaggacaagagaaauaaggccuacuucacaaagcgccuucccccgu
aaaugauaucaucucaacuuaguauuauacccacacccacccaagaacag
gguu

<<<.......<<<<..................................<.
.....>...............<<.....>>.............>>.>>..
...........<<....>>.................>>>...........
<<<.......>>>...<.<<......>>.>.<<<<<<.........<<<.
<<..<<....>>...>>.>>>...<<.<...<<<<.<.....<<<<<.<<
<..<<<.....>>>...<<<..<<<<........>>>>.>>>....>>>.
>>>>>...<<.......>>......>.>>>>......>.>>..<<<<<<.
...<<<<....>>>>.<<.<<<<<<<<<<.....<<..........>>..
...>>>>>>>>>>..>>..<<<<<<<<<<....(.>>>>>>>>>>...<<
<......>>>....<<<.<<<...<..............<....<<<...
......>>>....>.......<<<......>>>.................
...............>...>>>>>>........>>>>>>...<<<<<...
...>>>>>.........>>>>>>.......<<..<<<....>>><<<<..
<<<<........<<<<<..........>>>>>......<<<...<<<<..
.....................>>>>.......>>>.....<<......>>
.>>>>.....>>>>..<<<<...<<<<<<<<.<<.........<......
<<<<<<..<<<.........>>>...<<<<<<.............>>>>>
>....<<<.....<<<<..>>>>.<<<<<<.....>>>>>>.........
.>>>....>>.>>>>.....>..>>.>>>>>>>>>>>>.>>.........
..<<<<<........>>>>>.......<<..<<<.<<<....<<<<<.<<
<<<<..<<<<<.<<<<<<<<......>>>>>>>>>>>>>..<<<<.[.[.
>>>><<<<<.............>>>>>..<<.....<<<..<<<<.....
>>>>..>>>.....>>.........<<<....>>>............>>>
>..>>>>>>>..........<<<<<<<<<...<<.......>>...>>>>
>>>>>)....].].....<<<<<..<<<<<..<<.<<<<<<......>>>
>>>...>>.<<<<<.....>>>>>.....>>>>>......>>>>>.....
<<<<<<<....>>>.>>>>...>>>..>>>.>>.....<<<.<<<.....
............>>>.>>><<<<<...<<<....>>>.>>>>>......<
<<................>>>.................<<<<.......>
>>>.

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7o9m Structural basis for late maturation steps of the human mitoribosomal large subunit.
• Resolution: 2.6 Å
• Binding residue (original residue number in PDB): K25 N26 R27 A30 E31 H32 W34 L35 L39 R40 P42 K48 C54 R55 K59 A156 N158 A159 T160 G161 R163 W196 A197 S199 R216 I217 K225 S227 E229
• Binding residue (residue number reindexed from 1): K1 N2 R3 A6 E7 H8 W10 L11 L15 R16 P18 K24 C30 R31 K35 A132 N134 A135 T136 G137 R139 W172 A173 S175 R192 I193 K201 S203 E205

Enzymatic activity

• Enzyme Commision number: 2.1.1.-

Gene Ontology

Molecular Function

• GO:0008168 methyltransferase activity
• GO:0008650 rRNA (uridine-2'-O-)-methyltransferase activity

Biological Process

• GO:0000451 rRNA 2'-O-methylation
• GO:0001510 RNA methylation
• GO:0006364 rRNA processing
• GO:0031167 rRNA methylation
• GO:0032259 methylation
• GO:0042254 ribosome biogenesis
• GO:1902775 mitochondrial large ribosomal subunit assembly

Cellular Component

• GO:0005730 nucleolus
• GO:0005739 mitochondrion
• GO:0005759 mitochondrial matrix

External links

• PDB: RCSB:7o9m, PDBe:7o9m, PDBj:7o9m
• PDBsum: 7o9m
• PubMed: 34135318
• UniProt: Q9UI43|MRM2_HUMAN rRNA methyltransferase 2, mitochondrial (Gene Name=MRM2)