Structure of PDB 7o9k Chain n Binding Site BS01

Receptor Information

>7o9k Chain n (length=189) Species: 9606 (Homo sapiens)

SYRCRSAFKLLEVNERHQILRPGLRVLDCGAAPGAWSQVAVQKVNAAGTD
PSSPVGFVLGVDLLHIFPLEGATFLCPADVTDPRTSQRILEVLPGRRADV
ILSDMAPNATGFRDLDHDRLISLCLTLLSVTPDILQPGGTFLCKTWAGSQ
SRRLQRRLTEEFQNVRIIKPEASRKESSEVYFLATQYHG

Ligand information

>7o9k Chain A (length=1436)

gcuaaaccuagccccaaacccacuccaccuuacuaccacaaccuuagcca
aaccauuuacccaaauaaaguauaggcgauagaaauugggcgcaauagau
auaguaccgcaagggaaagaugaaaaaaccaagcauaauauagcaaggac
uaaccccuauaccuucugcauaaugaauuaacuagaaauaacuuugcaag
gagagccaaagcuaagacccccgaaaccagacgagcuaccuaagaacagc
uaaaagagcacacccgucuauguagcaaaauagugggaagauuuauaggu
agaggcgacaaaccuaccgagccuggugauagcugguuguccaagauaga
aucuuaguucaacuuuaaauuugcccacagaacccucuaaauccccuugu
aaauuuaacuguuaguccaaagaggaacagcucuuuggacacuaggaaaa
aaccuuguagagagaguaaaaaauuuaacccauaguaggccuaaaagcag
ccaccaauuaagaaagcguucaagcucaaaaaaaucccaaacauauaacu
gaacuccucacacccaauuggaccaaucuaucacccuauagaagaacuaa
uguuaguauaaguaacaugaaaacauucuccuccgcauaagccugcguca
gaucacugaacugacaauuaacagcccaauaucuacaaucaaccaacaag
ucauuauuacccucacugucaacccaacacaggcaugcucauaaggaaag
guuaaaaaaaguaaaaggaacucggcaaaucuuaccccgccuguuuacca
aaaacaucaccucuagcaucaccaguauuagaggcaccgccugcccagug
aucacuuguuccuuaaauagggaccuguaugaauggcuccacgaggguuc
agcugucucuuacuuuuaaccagugaaauugaccugcccgugaagaggcg
ggcauaacacagcaagacgagaagacccuauggagcuuuaauuuauuaau
gcaaacaccugcauuaaaaauuucgguuggggcgaccucggagcagaacc
caaccuccgagcaguacaugcuaagacuucaccagucaaagcgaaccuca
auugauccaauaacuugaccaacggaacaaguuacccuagggauaacagc
gcaauccuauucuagaguccauaucaacaauaggguuuacgaccucgaug
uuggaucaggacaucccgauggugcagccgcuauuaaagguucguuuguu
caacgauuaaaguccuacgugaucugaguucagaccggaguaauccaggu
cgguuucuaucuacuuucaaauuccucccuguacgaaaggacaagagaaa
uaaggccuacuucacaaagcgccuucccccguaaaugauaucaucucaac
uuaguauuauacccacacccacccaagaacaggguu

<<<.......<<<<...................................<
......>.........<.....<<.....>>....>..>>.>>.......
......<<....>>.................>>>...........<<<..
.....>>>...<.<<......>>.>.<<<<<<.........<<<.<<..<
<....>>...>>.>>>...<<.<...<<<<.<.....<<<<<.<<<..<<
<.....>>>...<<<..<<<<........>>>>.>>>....>>>.>>>>>
...<<.......>>......>.>>>>......>.>>..<<<<<<....<<
<<....>>>><<<.<<<<<<<<<<.....<<..........>>.....>>
>>>>>>>>..>>>.<<<<<<<<<<......>>>>>>>>>>...<<<....
..>>>....<<<.<<<...<............<....<<<.........>
>>....>.......<<<......>>>.......................>
...>>>>>>........>>>>>>...<<<<<......>>>>>........
.>>>>>><......<<..<<<....>>><<<<..<<<<........<<<<
<.........>>>>>......<<<...<<<<...................
....>>>>.......>>>.....<<......>>.>>>>.....>>>>..<
<<<...<<<<<<<<.<<.........<......<<<<<<..<<<......
...>>>...<<<<<<.............>>>>>>....<<<.....<<<<
..>>>>.<<<<<<.....>>>>>>..........>>>....>>.>>>>..
...>..>>.>>>>>>>>>>>>.>>.....>.....<<<<<........>>
>>>.......<<..<<<.<<.....<<<<<.<<<<<<..<<<<<.<<<<<
<<<......>>>>>>>>>>>>>..<<<<.....>>>><<<<<........
.....>>>>>..<<.....<<<..<<<<.....>>>>..>>>....>>..
.......<<<....>>>............>>>>..>>>>>>>.....<<<
<..<.<<<<<<...<<.......>>...>>>>>>...>.>>.>.>....<
<<<<<.<<<<<<..<<.<<<<<<......>>>>>>...>>.<<<<<....
.>>>>>.....>>>>>..>...>>>.>>>...<<<<<<<....>>>.>>>
>....>>..>>>.>>.....<<<.<<<.................>>>.>>
><<<<<...<<<....>>>.>>>>>......<<<................
>>>.................<<<<.......>>>>.

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7o9k Structural basis for late maturation steps of the human mitoribosomal large subunit.
• Resolution: 3.1 Å
• Binding residue (original residue number in PDB): A159 T160 G161 A197 R216 K225 E226 S227
• Binding residue (residue number reindexed from 1): A109 T110 G111 A147 R166 K175 E176 S177

Enzymatic activity

• Enzyme Commision number: 2.1.1.-

Gene Ontology

Molecular Function

• GO:0008168 methyltransferase activity
• GO:0008650 rRNA (uridine-2'-O-)-methyltransferase activity

Biological Process

• GO:0000451 rRNA 2'-O-methylation
• GO:0001510 RNA methylation
• GO:0006364 rRNA processing
• GO:0031167 rRNA methylation
• GO:0032259 methylation
• GO:0042254 ribosome biogenesis
• GO:1902775 mitochondrial large ribosomal subunit assembly

Cellular Component

• GO:0005730 nucleolus
• GO:0005739 mitochondrion
• GO:0005759 mitochondrial matrix

External links

• PDB: RCSB:7o9k, PDBe:7o9k, PDBj:7o9k
• PDBsum: 7o9k
• PubMed: 34135318
• UniProt: Q9UI43|MRM2_HUMAN rRNA methyltransferase 2, mitochondrial (Gene Name=MRM2)