Structure of PDB 8wi7 Chain m Binding Site BS01

Receptor Information
>8wi7 Chain m (length=121) Species: 246196 (Mycolicibacterium smegmatis MC2 155) [Search protein sequence] [Download receptor structure] [Download structure with residue number starting from 1] [View receptor structure]
PTIQQLVRKGRRDKIAKVKTAALKGSPQRRGVCTRVYTTTPKKPNSALRK
VARVKLTSQVEVTAYIPGEGHNLQEHSMVLVRGGRVKDLPGVRYKIIRGS
LDTQGVKNRKQARSRYGAKKE
Ligand information
>8wi7 Chain a (length=1515) [Search RNA sequence] [Download ligand structure] [Download structure with residue number starting from 1] [View ligand structure]
uggagaguuugauccuggcucaggacgaacgcuggcggcgugcuuaacac
augcaagucgaacggaaaggcccuuucggggguacucgaguggcgaacgg
gugaguaacacgugggugaucugcccugcacuuugggauaagccugggaa
acugggucuaauaccgaauacacccugcuggucgcauggccugguagggg
aaagcuuuugcggugugggaugggcccgcggccuaucagcuuguuggugg
ggugauggccuaccaaggcgacgacggguagccggccugagagggugacc
ggccacacugggacugagauacggcccagacuccuacgggaggcagcagu
ggggaauauugcacaaugggcgcaagccugaugcagcgacgccgcgugag
ggaugacggccuucggguuguaaaccucuuucagcacagacgaagcgcaa
gugacgguaugugcagaagaaggaccggccaacuacgugccagcagccgc
gguaauacguaggguccgagcguuguccggaauuacugggcguaaagagc
ucguaggugguuugucgcguuguucgugaaaacucacagcuuaacugugg
gcgugcgggcgauacgggcagacuagaguacugcaggggagacuggaauu
ccugguguagcgguggaaugcgcagauaucaggaggaacaccgguggcga
aggcgggucucugggcaguaacugacgcugaggagcgaaagcguggggag
cgaacaggauuagauacccugguaguccacgccguaaacgguggguacua
gguguggguuuccuuccuugggauccgugccguagcuaacgcauuaagua
ccccgccuggggaguacggccgcaaggcuaaaacucaaaggaauugacgg
gggcccgcacaagcggcggagcauguggauuaauucgaugcaacgcgaag
aaccuuaccuggguuugacaugcacaggacgccggcagagaugucgguuc
ccuuguggccugugugcagguggugcauggcugucgucagcucgugucgu
gagauguuggguuaagucccgcaacgagcgcaacccuugucucauguugc
cagcacguuaugguggggacucgugagagacugccggggucaacucggag
gaagguggggaugacgucaagucaucaugccccuuauguccagggcuuca
cacaugcuacaauggccgguacaaagggcugcgaugccgugagguggagc
gaauccuuucaaagccggucucaguucggaucggggucugcaacucgacc
ccgugaagucggagucgcuaguaaucgcagaucagcaacgcugcggugaa
uacguucccgggccuuguacacaccgcccgucacgucaugaaagucggua
acacccgaagccgguggccuaacccuuguggagggagccgucgaaggugg
gaucggcgauugggacgaagucguaacaagguagccguaccggaaggugc
ggcuggaucaccucc
....<<<<..[.((((.>>>>.<<<<.<<<<<..<<<<<<<<.....<<<
.<<<..<<<..<<.<..<<<<<<<....>>>>>.>>>..>>>>>......
<<........<<<<<<<..<<...<<<<<<<<<<<<.....<<<<<<...
.>>>>>>......>>>>.....<<<<<<<<<<<<..>>>>>.>>>>>>>.
...<<....>>>>>>>>>>..>>>>>>>>>.<<<....<<<..<<<<<<<
<.......>>>>>>>>>>>......>>>..<<<<<<<<....>>>>...>
>>>.>>.<<<<<.<.........>>>>>>.<<<<....>>>>...>>>>>
>.........<<<....<<<<....>>>>..>>>..>>.>>>>>>..<<<
<......<<<<....>>>>.....>>>>...<.<<<<<......<<....
>>.......>>>>>....>..<<<<<(((...<<<<<.....<<.)))>>
.......>>>>>>>>>>..>>>>>>>>>..........<<<((.....<<
<<...<<<.<<<<<<<.<<<<<<<<<<.....<<<<<<<.....>>>>>>
>...>>>>>>>..>>>>>>>>>>...<<<<<<<<...<<<<<<<....<<
<<<<<<<..<<<......>>>.....>>>>>>>>>...........<<..
..>>.>>>>>>>..>>>>>.>>>...>>>...>>>>....<<<<<<...<
<...<<<<.<.....>.>>>>...>>>>>>>>..........<<<<<<..
<<<<<<<<<<<<.......>>>>>>>>>>>>...<<..))>>.....>>>
>>>.>>>.<<<......<<<<....>>>>....>>>..)))).]<<<<<.
<<<<<<<.<<.<<<<<<..<<<<<<<<<<......<<........>>...
.......<<<<<<<......<<<<<<<...<<<<<<<....>>>>>>>.<
<.....>>.>>>>>>>.<<<.<<<..<<<<<<.......<<<<<<<<<..
..>>>..<<<<......>>>>..>>>>>>.....<<<<.<<<<<<....<
<..<<<......>>>>>.....>>>>>>.....<<<<<.....>>>>>..
......>>>>.........>>>...>>>>>>>>>...>>>>>>>...>>.
>>>>>>>>.....<<<<<<<.....<<<..<<..<<<<....>>>>..>>
....>>>......>>>>>>>......<....<<<<<<<........>>>>
>>>....>.....>>>>>>....<<<<<<<..........>>>>>>>...
...>>...>>>>>>>>>>.>>....<..<<.<.<<<<.<<<..<<<<<<.
.<<<<....<.<<<<..<<<..<<.....>>.>>>.>>>>.>...>>>>.
.>>>>>>..>>>.>>>>..>.>>...>.....<<<<<<<<<....>>>>>
>>>>...........
Receptor-Ligand Complex Structure
Global viewLocal viewStructure summary

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PDB8wi7 Cryo- EM structure of the mycobacterial 70S ribosome in complex with ribosome hibernation promotion factor RafH.
Resolution3.5 Å
Binding residue
(original residue number in PDB)		P2 T3 Q6 R9 R12 R13 D14 K15 S27 P28 Q29 R30 R31 K43 K44 N46 S47 A48 L49 R50 K51 Y66 G69 E70 G84 K88 D89 R94 R110 K111 Q112 A113 R114 S115 R116 K120 K121
Binding residue
(residue number reindexed from 1)		P1 T2 Q5 R8 R11 R12 D13 K14 S26 P27 Q28 R29 R30 K42 K43 N45 S46 A47 L48 R49 K50 Y65 G68 E69 G83 K87 D88 R93 R109 K110 Q111 A112 R113 S114 R115 K119 K120
Enzymatic activity
Enzyme Commision number ?
Gene Ontology
Molecular Function
GO:0000049 tRNA binding
GO:0003735 structural constituent of ribosome
GO:0019843 rRNA binding
Biological Process
GO:0006412 translation
Cellular Component
GO:0005840 ribosome
GO:0015935 small ribosomal subunit
GO:1990904 ribonucleoprotein complex

View graph for
Molecular Function
View graph for
Biological Process
View graph for
Cellular Component
External links
PDB RCSB:8wi7, PDBe:8wi7, PDBj:8wi7
PDBsum8wi7
PubMed38245551
UniProtA0QS96|RS12_MYCS2 Small ribosomal subunit protein uS12 (Gene Name=rpsL)

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