Structure of PDB 7ohv Chain m Binding Site BS01

Receptor Information

>7ohv Chain m (length=583) Species: 559292 (Saccharomyces cerevisiae S288C)

DGSDRIIKPEINPVYDSDDSDAETMPHIGYDINGKRIMRPLTKEELELIS
KIQRNEQINPYEPLIDWFTRHEEVMPLTAVPEPKRRFVPSKNEAKRVMKI
VRAIREGRIIPPKKLKEMKEKEKIENYQYDLWGDSTETNDHVMHLRAPKL
PPPTNEESYNPPEEYLLSPEEKEAWENTEYSERERNFIPQKYSALRKVPG
YGESIRERFERSLDLYLAPRVRKNKLNIDPNSLIPELPSPKDLRPFPIRC
STIYAGHKGKVRTLSIDPSGLWLATGSDDGTVRVWEILTGREVYRTTPDY
HIECIEWNPDANNGILAVAVGENIHLIVPPIFGYDIENNGKTKIEDGFAQ
WNKPSQKQLEKDICITISCKKTVKKLSWHRKGDYFVTVQPDSGNTSVLIH
QVSKHLTQSPFKKSKGIIMDAKFHPFKPQLFVCSQRYVRIYDLSQQILVK
KLLPGARWLSKIDIHPRGDNLIASSFDKRVLWHDLDLASTPYKTLRYHEK
AVRSVNFHKKLPLFSSAADDGTIHVFHATVYDDMMKNPMIVPLKKLTGHK
VINSLGVLDAIWHPREAWLFSAGADNTARLWTT

Ligand information

>7ohv Chain 1 (length=2141)

guuugaccucaaaucagguaggaguacccgcugaacuuaagcauaucaau
aagcggaggaaaagaaaccaaccgggauugccuuaguaacggcgagugaa
gcggcaaaagcucaaauuugaaaucugguaccuucggugcccgaguugua
auuuggagagggcaacuuuggggccguuccuugucuauguuccuuggaac
aggacgucauagagggugagaaucccguguggcgaggagugcgguucuuu
guaaagugccuucgaagagucgaguuguuugggaaugcagcucuaagugg
gugguuccaucuaaagcuaaauauuggcgagagaccgauagcgaacaagu
acagugauggaaagaugaaaagaacuuugaaaagagagugaaaaaguacg
ugaaauuguugaaagggaagggcauuugaucagacaggccagcaucaguu
uugguggcaggauaaauccauaggaauguagcuugccucgguaaguauua
uagccugugggaauacugccagcugggacugaggacugcgacguaaguca
aggaugcuggcauaaugguuauaugccgcccgucuugaaacacggaccaa
ggagucuaacgucuaugcgaguguuuggguguaaaacccauacgcguaau
gaaagugaacguagguuggggccucgcaagaggugcacaaucgaccgauc
cugaggauggauuugaguaagagcauagcuguugggacccgaaagauggu
gaaagccagcugguggaccaucuaguagcugguuccugccgaaguuuccc
uaggauagcagaagcucguaucaguuuuauguaaaaugaagagcuuuuag
ugggccauuuuugguaagcagaacuggcgaugcgggaugaaccgaacgua
gaguuaaggugccggaauacacgcucaucagacaaaagguguuaguucau
cuagacagccggacgguggccauggaagucggaauccgcuaaggagugug
uaacaacucaccggccgaaugaacuagcccugaaggauggcgcucaagcg
uguuaccuauacucuaccgucaggguugauaugaugcccugacgaguagg
caggcguggaggucagugacgaagccuagaccguaaggucgggucgaacg
gccucuagugcagaucuuggugguaguagcauucaaaugagaacuuugaa
gacugaaggggaaagguuccacgucaacagcaguuggacguggguuaguc
gauccuaagagauggggaagcuccguggccugauuuuaugcaggccacca
ucgaaagggaauccgguuaagauuccggaaccuggauauggauucuucac
gguaacguaacugaauguggagacgucggcgcgagcccugggaggaguua
ucuuuucuucuuaacagcuuaucaccccggagguuuauccgggauggggu
cuuauggcuggaagaggccagcacugcuggcuccgguggcuugugacggc
cugaaaauccacaggaaggaauaguuuucaugccaggucguacugcucca
aggugacagccucuaguugauagaauaauguacuacuaucuagcgaaacc
acagccaagggaacgggcuuggcagaaucagcgggacucuaguuugacau
uauugucaggugggagugcuuguggcagucaagcgagcgacauugcuauc
auaccgaagcagaauucgguaagcgucuaauaccuacugaugaauguuac
cgcaauaguaauugaacuuaguacgagaggaacaguucauucggauaauu
gguuuuugcggcugucugaucaggcaugccgcgaagcuaccauccgcugg
auuauggcugaacgccucuaagucagaauccaugcuagaacgcggugauu
ucuuugcuccacacaauauagauggauacgaauaaggcguccuuguggcg
ucgcugaaccauagcaggcuagcaacggugcacuuggcggaaaggccuug
ggugcuugcuggcgaauugcaaugucauuuugcguggggauaaaucauuu
guauacgacuuagauguacaacgggguauuguaagcaguagaguagcugu
guuacgaucugcugagauuaagccuuuguugucugauuugu

...........................<<<<<.....<<....>>.....
.>>>>>...........<<....>>..<<<<<......<<.....>>...
..>>>>>..<<<..<........<<.<<<<<<...>>>>>>.>>......
..>....<<<<<<<.<<<<<<<<<<<<<<<<<<<<<....<<<<......
>>>>.((......<<<.....))>>>..>.>>>>>>>>..>>>>>>>>..
..>>>>>>>>>>>........<<<<<<<........>>>>>>>.....<<
<<<<..>>>>>>..>>>.................................
.<<....>>...............<<<<....>>>>..............
....................<<<<<.<<<<<......<<<<<<<.<<<<<
<<<<<<<<<<......<<<<<<<<......<<<<<<....>>>>>>....
...>>>>>>>.>...>>>>>.>>>>>>>>>>........<<<....>>>.
....>>>>>>>.....>>>>>.>>>>>..<<<<........>>>><<<<<
<....<<<<<<.<<<<<<..<<<...<<<<.....>>>>...>>>...<<
....>>.......<<<<<..<<<<<....>>>>>...>>>>>.<<<<<<<
<.......>>>.>>>.>>....>>>>>>.>>>>>>..<<.<..<<<<<<<
....<<<<..>>>>..>>>>>>>.....>.>>..<<<<<<....<.<...
....>.><<<<<<<<<....<<<.<<<<<..>>>>>>>>..>>>>>>>.>
>..<<<<.<<<<.......>>>>.>>>>....<<<......>>>...<<<
<<<<..<<<<(((.....<<<<<<.....<<.....<<<.<<<<<<<<<<
.......<<<<<.<<....<<.........>>....>>.........<<.
.....>>...>>>>>...>>>>>>>>>>>>>.......))).>>...>>>
>>>.>>>>..>>>>>>><<<<<<<<<.........>>>>>>>>>....>>
>>>><..<<<<<<<.<<......<<<<.<<<<....>>>>>>>>...>>>
>>>>>>...>.....>>>>>><<.....<<.<<<<<<.......>>>>>>
<<<<<..<<<...<<...<<<<<<...<<.....>>.>>>>>>....((<
<<<<<...<<....<<<....>>>..<<<<<<........>>>>>>....
>>....>>...<<<<<........>>>>>))<<<<.<<<<<<......<<
<....>>>...<...<<<<<<...<<<<.<<<<<<<<<<<<<<<<<<...
....>>>>>><...<<<<<....<<<<<<..<<.....>>....>>>>>>
.....>>>>>....><<<<<<<...>>>>>>>>>>>.>>>>>>>>.>>>>
.......>>>>>>...>........>>.>>>>>>>>>>>>..>>..>>>.
<<<<....>>>>.>>>>>..<<<<.....<<<.>>>..>>>>>>....>>
....<<<<<........>>>>>....<<<<<..<<<<<<<..<<<<<<<.
...>>>>>>>..>>>>>........<<<<...<<..>>...>>>>....<
.<<<<<<<......>>>>>>>...>........>>.>>>>>....<<<<<
<<<..<<<<.<.<<<<<<...............>>>>>>.><<<<<...<
<<<....<<<<<<<<<<....>>>>>.>>>>>...>>>>..>>>>>.<<<
<...<<<<<...........>>>>>...>>>>.>>>>....>>>>>>>>.
......<<<<<<.<<<.<<....<<..........<<<<<<.....>>>>
>>......>>.......<<<<<<...<<<<<.<..<<<......>>>..>
.>>>>>.>>>>>>.............>>.>>>.>>>>>>..<<<<<.<..
...........>.....<<<<<<<<<.<<<....<<<<....<<<<....
.>>>>...>>>>...>>>....>>>>.>>>>>..>>>>>..

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7ohv Analysis of subunit folding contribution of three yeast large ribosomal subunit proteins required for stabilisation and processing of intermediate nuclear rRNA precursors.
• Resolution: 3.9 Å
• Binding residue (original residue number in PDB): P260 E261 P262 K263 R264 R265 F266 V267 S269 E272 K274 R275 M277 R281 R287 I288 K402 L405 N406 I407 G617 N618 K637 K639 Q659 R660 Y661 R681 W682 R703 R720
• Binding residue (residue number reindexed from 1): P81 E82 P83 K84 R85 R86 F87 V88 S90 E93 K95 R96 M98 R102 R108 I109 K223 L226 N227 I228 G393 N394 K413 K415 Q435 R436 Y437 R457 W458 R479 R496

Gene Ontology

Molecular Function

• GO:0003674 molecular_function
• GO:0003677 DNA binding
• GO:0005515 protein binding
• GO:0043021 ribonucleoprotein complex binding
• GO:0070180 large ribosomal subunit rRNA binding

Biological Process

• GO:0000463 maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
• GO:0000466 maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
• GO:0006364 rRNA processing
• GO:0042254 ribosome biogenesis
• GO:0042273 ribosomal large subunit biogenesis

Cellular Component

• GO:0005634 nucleus
• GO:0005654 nucleoplasm
• GO:0005730 nucleolus
• GO:0030687 preribosome, large subunit precursor
• GO:0070545 PeBoW complex

External links

• PDB: RCSB:7ohv, PDBe:7ohv, PDBj:7ohv
• PDBsum: 7ohv
• PubMed: 34813592
• UniProt: Q04660|ERB1_YEAST Ribosome biogenesis protein ERB1 (Gene Name=ERB1)