Structure of PDB 8p18 Chain l Binding Site BS01

Receptor Information

>8p18 Chain l (length=154) Species: 562 (Escherichia coli)

PRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVYSALETLA
QRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAM
RWIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAF
AHYR

Ligand information

>8p18 Chain 2 (length=1536)

aaauugaagaguuugaucauggcucagauugaacgcuggcggcaggccua
acacaugcaagucgaacgguaacaggaagaauucuuugcugacgaguggc
ggacgggugaguaaugucugggaaacugccugauggagggggauaacuac
uggaaacgguagcuaauaccgcauaacgucgcaagaccaaagagggggac
cuucgggccucuugccaucggaugugcccagaugggauuagcuaguaggu
gggguaacggcucaccuaggcgacgaucccuagcuggucugagaggauga
ccagccacacuggaacugagacacgguccagacuccuacgggaggcagca
guggggaauauugcacaaugggcgcaagccugaugcagccaugccgcgug
uaugaagaaggccuucggguuguaaaguacuuucagcggggaggaaggga
guaaaguuaauaccuuugcucauugacguuacccgcagaagaagcaccgg
cuaacuccgugccagcagccgcgguaauacggagggugcaagcguuaauc
ggaauuacugggcguaaagcgcacgcaggcgguuuguuaagucagaugug
aaauccccgggcucaaccugggaacugcaucugauacuggcaagcuugag
ucucguagagggggguagaauuccagguguagcggugaaaugcguagaga
ucuggaggaauaccgguggcgaaggcggcccccuggacgaagacugacgc
ucaggugcgaaagcguggggagcaaacaggauuagauacccugguagucc
acgccguaaacgaugucgacuuggagguugugcccuugaggcguggcuuc
cggagcuaacgcguuaagucgaccgccuggggaguacggccgcaagguua
aaacucaaaugaauugacgggggcccgcacaagcgguggagcaugugguu
uaauucgaugcaacgcgaagaaccuuaccuggucuugacauccacggaag
uuuucagagaugagaaugugccuucgggaaccgugagacaggugcugcau
ggcugucgucagcucguguugugaaauguuggguuaagucccgcaacgag
cgcaacccuuauccuuuguugccagcgguccggccgggaacucaaaggag
acugccagugauaaacuggaggaagguggggaugacgucaagucaucaug
gcccuuacgaccagggcuacacacgugcuacaauggcgcauacaaagaga
agcgaccucgcgagagcaagcggaccucauaaagugcgucguaguccgga
uuggagucugcaacucgacuccaugaagucggaaucgcuaguaaucgugg
aucagaaugccacggugaauacguucccgggccuuguacacaccgcccgu
cacaccaugggaguggguugcaaaagaaguagguagcuuaaccuucggga
gggcgcuuaccacuuugugauucaugacuggggugaagucguaacaaggu
aaccguaggggaaccugcgguuggaucaccuccuua

........<<<<..[.((((.>>>>.<<<<.<<<<<..<<<<<<<<....
.<<<.<<<..<<<..<<.<<..<<<<<<<<..>>>>>.>>>>>..>>>>>
......<<.......<<<<<<<..<<...<<<<<<<.<.<<.....<<<<
<......>>>>>......>>.>.....<<<....>>>....<<<<<<..<
<....>>>>>>>>.>>>>>>>..>>.>>>>>>><<<....<<<..<<<<<
<<.........>>>>>>>>>>......>>>..<<<<<<<<....>>>>..
.>>>>.>>.<<<<<.<.........>>>>>>.<<<<....>>>>...>>>
>>>.........<<<....<<<<....>>>>..>>>..>>.>>>>>>..<
<<<......<<<<....>>>>.....>>>>...<.<<<<<......<.<<
<<<<<<.......>>>>>>>>.>........>>>>>....>..<<<<<((
(...<<<<<.....<<.)))>>.......>>>>>>>>>>..>>>>>>>>>
..........<<<((.....<<<<...<<<.<<<<<<<.<<<<<<<<<<.
.....<<<<<<.....>>>>>>....>>>>>>>>..>>>>>>>>>...<<
<<<<<<...<<<<<<<....<<<<<<<<...<<<......>>>......>
>>>>>>>...........<<....>>.>>>>>>>..>>>>.>>>>...>>
>...>>>>....<<<<<<...<<...<<<<.........>>>>...>>>>
>>>>..........<<<<<<.<<<<<<<<<<<<<.....>>>>>>>>>>>
>>..<<..))>>.....>>>>>>.>>>.<<<......<<<<....>>>>.
...>>>..)))).]<<<<<.<<<<<<<.<<.<<<<<<..<<<<<<<<<<.
.....<<........>>..........<<<<<<<......<<<<<<<..<
<<<<<<....>>>>>>>...<......>..>>>>>.>>.<<<.<<<..<<
<<<<.......<<<<<<<<<....>>>..<<<<......>>>>..>>>>>
>.....<<<<.<<<<<<<...<<..<<<.....>>>>>....>>>>>>>.
....<<<<<.....>>>>>........>>>>.........>>>...>>>>
>>>>>...>>>>>>>...>>.>>>>>>>>.....<<<<<<<.....<<<.
.<<...<<<....>>>...>>....>>>.....>>>>>>>..........
.<<<<<<<........>>>>>>>..........>>>>>>....<<<<<<<
.........>>>>>>>......>>...>>>>>>>>>>.>>....<..<<.
<.<<<<.<<<..<<<<<<<<<<<<....<<<<<<.<<<<..<<....>>.
>>>>>>>>>>...>>>>>>>>>>>>..>>>.>>>>..>.>>...>.....
<<<<<<<<<....>>>>>>>>>..............

Receptor-Ligand Complex Structure

Structure summary

• PDB: 8p18 The compensatory mechanism of a naturally evolved E167K RF2 counteracting the loss of RF1 in bacteria
• Resolution: 2.77 Å
• Binding residue (original residue number in PDB): P2 R3 R4 I7 R10 K25 N28 L30 M31 D33 G34 K35 K36 S37 T38 K76 R79 R95 R102 R109 K114 M116 R155
• Binding residue (residue number reindexed from 1): P1 R2 R3 I6 R9 K24 N27 L29 M30 D32 G33 K34 K35 S36 T37 K75 R78 R94 R101 R108 K113 M115 R154

Gene Ontology

Molecular Function

• GO:0000049 tRNA binding
• GO:0003729 mRNA binding
• GO:0003735 structural constituent of ribosome
• GO:0005515 protein binding
• GO:0019843 rRNA binding

Biological Process

• GO:0000028 ribosomal small subunit assembly
• GO:0002181 cytoplasmic translation
• GO:0006412 translation
• GO:0017148 negative regulation of translation

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0016020 membrane
• GO:0022627 cytosolic small ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:8p18, PDBe:8p18, PDBj:8p18
• PDBsum: 8p18
• UniProt: P02359|RS7_ECOLI Small ribosomal subunit protein uS7 (Gene Name=rpsG)