Structure of PDB 8jsh Chain l Binding Site BS01

Receptor Information
>8jsh Chain l (length=205) Species: 562 (Escherichia coli) [Search protein sequence] [Download receptor structure] [Download structure with residue number starting from 1] [View receptor structure]
ARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGARKPRLSDY
GVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNV
VYRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKK
QSRVKAALELAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIV
ELYSK
Ligand information
>8jsh Chain g (length=1074) [Search RNA sequence] [Download ligand structure] [Download structure with residue number starting from 1] [View ligand structure]
aauugaagaguuugaucauggcucagauugaacgcuggcggcaggccuaa
cacaugcaagucgaacgguaacaggaagaagcuugcuucuuugcugacga
guggcggacgggugaguaaugucugggaaacugccugauggagggggaua
acuacuggaaacgguagcuaauaccgcauaacgucgcaagaccaaagagg
gggaccuucgggccucuugccaucggaugugcccagaugggauuagcuag
uaggugggguaacggcucaccuaggcgacgaucccuagcuggucugagag
gaugaccagccacacuggaacugagacacgguccagacuccuacgggagg
cagcaguggggaauauugcacaaugggcgcaagccugaugcagccaugcc
gcguguaugaagaaggccuucggguuguaaaguacuuucagcggggagga
agggaguaaaguuaauaccuuugcucauugacguuacccgcagaagaagc
accggcuaacuccgugccagcagccgcgguaauacggagggugcaagcgu
uaaucggaauuacugggcguaaagcgcacgcaggcgguuuguuaagucag
augugaaauccccgggcucaaccugggaacugcaucugauacuggcaagc
uugagucucguagagggggguagaauuccagguguagcggugaaaugcgu
agagaucuggaggaauaccgguggcgaaggcggcccccuggacgaagacu
gacgcucaggugcgaaagcguggggagcaaacaggauuagauacccuggu
aguccacgccguaaacgaugucgacuuggagguugugcccuugaggcgug
gcuuccggagcuaacgcguuaagucgaccgccuggggaguacggccgcaa
gguuaaaacucaaaugaauugccuuguacacaccgcccgucacaccaugg
gaguggguugcaaaagaaguagguagcuuaaccuucgggagggcgcuuac
cacuuugugauucaugacuggggugaagucguaacaagguaaccguaggg
gaaccugcgguuggaucaccuccu
.......<<<<..[.((((.>>>>.<<<<.<<<<<..<<<<<<<<.....
<<<.<<<..<<<..<<.<......<................>.....>..
>>>>>......<<.......<<<<<<<..<<...<<<<<<<.<.<<....
.<.<<........>>.>......>>.>.....<<<....>>>....<<<<
<...<<....>>.>>>>>.>>>>>>>..>>.>>>>>>><<<....<<<..
<<<<<<<.........>>>>>>>>>>......>>>..<<<<<<<<....>
>>>...>>>>.>>.<<<<<.<.........>>>>>>.<<<......>>>.
..>>>>>>.........<<<....<<<<....>>>>..>>>..>>.>>>>
>>..<<<<.......<<<....>>>......>>>>..<<.<<<<<.....
...<<<.<<<<.......>>>>.>>>..........>>>>>....>>.<<
<<<(((...<<<<<.....<<.)))>>.......>>>>>>>>>>..>>>>
>>>>>..........<<<((.....<<<<...<<<.<<<<<<<.<<<<<<
<<<.......<<<<<<.....>>>>>>.....>>>>>>..>>>>>>>>>>
...<<<<<<<<...<<<<<<<....<<<<<<.....<<<......>>>..
......>>>>>>...........<<....>>.>>>>>>>..>>>>.>>>>
...>>>...>>>>....<<<<<<<...<...<<<<.........>>>>..
.>>>>>>>>..........<<<<<<.<<<<<<<....<<.....>>....
>>>>>>>..<<..))>>.....>>>>>>.>>>.<<<......<<<<....
>>>>....>>>..)))).]<.........>.......<<...<<<<...<
.......<..........<<<<<<.<<<<..<<....>>.>>>>>>>>>>
........>........>...>>>>....>>..........<<<<.<<<.
...>>>.>>>>.............
Receptor-Ligand Complex Structure
Global viewLocal viewStructure summary

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PDB8jsh Initiation factor 3 bound to the 30S ribosomal subunit in an initial step of translation.
Resolution4.4 Å
Binding residue
(original residue number in PDB)		A1 R2 K7 L8 K9 R12 R13 K21 G23 R25 K30 K32 G38 Q39 H40 R43 L54 Q58 R61 R62 L67 E68 Q70 R80 R114 Q115 S118 H119 K120 R127 V129 N130 I131 S133 Y134 K147 R153 E201 K205
Binding residue
(residue number reindexed from 1)		A1 R2 K7 L8 K9 R12 R13 K21 G23 R25 K30 K32 G38 Q39 H40 R43 L54 Q58 R61 R62 L67 E68 Q70 R80 R114 Q115 S118 H119 K120 R127 V129 N130 I131 S133 Y134 K147 R153 E201 K205
Enzymatic activity
Enzyme Commision number ?
Gene Ontology
Molecular Function
GO:0000900 mRNA regulatory element binding translation repressor activity
GO:0003723 RNA binding
GO:0003735 structural constituent of ribosome
GO:0005515 protein binding
GO:0019843 rRNA binding
GO:0048027 mRNA 5'-UTR binding
Biological Process
GO:0000028 ribosomal small subunit assembly
GO:0002181 cytoplasmic translation
GO:0006353 DNA-templated transcription termination
GO:0006412 translation
GO:0006417 regulation of translation
GO:0031564 transcription antitermination
GO:0042254 ribosome biogenesis
GO:0042274 ribosomal small subunit biogenesis
GO:0045947 negative regulation of translational initiation
GO:0046677 response to antibiotic
GO:1990145 maintenance of translational fidelity
Cellular Component
GO:0005737 cytoplasm
GO:0005829 cytosol
GO:0005840 ribosome
GO:0015935 small ribosomal subunit
GO:0022627 cytosolic small ribosomal subunit
GO:1990904 ribonucleoprotein complex

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:8jsh, PDBe:8jsh, PDBj:8jsh
PDBsum8jsh
PubMed38148682
UniProtP0A7V8|RS4_ECOLI Small ribosomal subunit protein uS4 (Gene Name=rpsD)

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