Structure of PDB 8cam Chain l Binding Site BS01

Receptor Information
>8cam Chain l (length=136) Species: 679895 (Escherichia coli BW25113) [Search protein sequence] [Download receptor structure] [Download structure with residue number starting from 1] [View receptor structure]
MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTARQIEAAR
RAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGK
VLYEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM
Ligand information
>8cam Chain a (length=2690) [Search RNA sequence] [Download ligand structure] [Download structure with residue number starting from 1] [View ligand structure]
gguuaagcgacuaagcguacacgguggaugcccuggcagucagaggcgau
gaaggacgugcuaaucugcgauaagcgucgguaaggugauaugaaccguu
auaaccggcgauuuccgaauggggaaacccaguguguuucgacacacuau
cauuaacugaauccauagguuaaugaggcgaaccgggggaacugaaacau
cuaaguaccccgaggaaaagaaaucaaccgagauucccccaguagcggcg
agcgaacggggagcagcccagagccugaaucaguguguguguuaguggaa
gcgucuggaaaggcgcgcgauacagggugacagccccguacacaaaaaug
cacaugcugugagcucgaugaguagggcgggacacgugguauccugucug
aauauggggggaccauccuccaaggcuaaauacuccugacugaccgauag
ugaaccaguaccgugagggaaaggcgaaaagaaccccggcgaggggagug
aaaaagaaccugaaaccguguacguacaagcagugggagcacgcuuaggc
gugugacugcguaccuuuuguauaaugggucagcgacuuauauucuguag
caagguuaaccgaauaggggagccgaagggaaaccgagucuuaacugggc
guuaaguugcaggguauagacccgaaacccggugaucuagccaugggcag
guugaagguuggguacacuaacuggaggaccgaaccgacuaauguugaaa
aauuagcggaugacuuguggcugggggugaaaggccaaucaaaccgggag
auagcugguucuccccgaaagcuauuuagguagcgccucgugaauucauc
uccggggguagagcacuguuucggcaacccgaugcaaacugcgaauaccg
gagaauguuaucacgggagacacacggcgggugcuaacguccgucgugaa
gagggaaacaacccagaccgccagcuaaggucccaaagucaugguuaagu
gggaaacgaugugggaaggcccagacagcgucgagucggccugcgcggaa
gauguaacggggcuaaaccaugcaccgaagcugcggcagcgacgcguugu
uggguaggggagcguucuguaagccugcgaaggugugcugugaggcaugc
uggagguaucagaagugcgaaugcugacauaaguaacgauaaagcgggug
aaaagcccgcucgccggaagaccaaggguuccuguccaacguuaaucggg
gcagggugagucgaccccuaaggcgaggccgaaaggcguagucgauggga
aacagguuaauauuccuguacuugguguuacugcgaaggggggacggaga
aggcuauguuggccgggcgacgguugucccgguuuaagcguguaggcugg
uuuuccaggcaaauccggaaaaucaaggcugaggcgugaugacgaggcac
uacggugcugaagcaacaaaugcccugcuuccaggaaaagccucuaagca
ucagguaacaucaaaucguaccccaaaccgacacagguggucagguagag
aauaccaaggcgcuugagagaacucgggugaaggaacuaggcaaaauggu
gccguaacuucgggagaaggcacgcugauauguaggugaggucccucgcg
gauggagcugaaaucagucgaagauaccagcuggcugcaacuguuuauua
aaaacacagcacugugcaaacacgaaaguggacguauacggugugacgcc
ugcccggugccggaagguuaauugaugggguuagcgcaagcgaagcucuu
gaucgaagccccgguaaacggcguaagguagcgaaauuccuugucgggua
aguuccgaccugcacgaauggcguaaugauggccaggcugucuccacccg
agacucagugaaauugaacucgcugugaagaugcaguguacccgcggcaa
gacggaaagaccccgugaaccuuuacuauagcuugacacugaacauguuc
uaacguugacccguaauccggguugcggacagugucugguggguaguuug
acuggggcggucuccuccuaaagaguaacggaggagcacgaagguuggcu
aaucagguuagugcaauggcauaagccagcuugacugcgagcgugacggc
gcgagcaggugcgaaagcaggucauagugauccggugguucugaauggaa
gggccaucgcucaacggauaaaagguacuccggggauaacaggcugauac
cgcccaagaguucauaucgacggcgguguuuggcaccucgaugucggcuc
aucacauccuggggcugaaguaggucccaaggguauggcuguucgccauu
uaaagugguacgcgagcuggguuuagaacgucgugagacaguucgguccc
uaucugccgugggcgcuggagaacugaggggggcugcuccuaguacgaga
ggaccggaguggacgcaucacugguguucggguugucaugccaauggcac
ugcccgguagcuaaaugcggaagagauaagugcugaaagcaucuaagcac
gaaacuugccccgagaugaguucucccugacccuuuaaggguccugaagg
aacguugaagacgacgacguugauaggccggguguguaagcgcagcgaug
cguugagcuaaccgguacuaaugaaccgugaggcuuaacc
<<<<<<<<......<<<<<<<<<<<.....[[..[[<<<<<<<[[<<...
...<<<<<<....(((.>>>.....<<<<<<<..<<<)))....>>>...
...>>>>>>>...>>>....<<<....>>><<<<<<<....>>>>>>>.<
<<<<<<<<.........>>>>>>>>>.>>...<<<<<<.....<<....>
>......>>>>>.>.........<<....>>..<<<<<<.....<<....
.>>....>>>>>>..(((..<<<<.<....<<<<<<<<<<<<<.<<<...
<<<<<......>>>>>((......<<<.....))>>>...>>>...>>>>
>>>>>>>>>..>>>>>........<<<<<<<<.........>>>>>>>>.
.....<<<<<..<<<<>>>>>..)))......]].>>>>>>>.]].....
..........<<....>>....]].........<<<<.....>>>>....
..............>>>>>>>>>>>.......<<<<..<<<<<<....>>
>>>>..>>>>...<............>.<<<<<<....<<<<<<<<<<<<
<..<<<...<<.....>>...>>>...<<....>>..<<<<.....>>>>
.....>>>>>>>>>>>>>..<<.<...<<<<<<.....<<<<<<<<<..<
<<<...<<<<<<.....>>>>>>.>...>>>...<<<.<<<<<.......
.>>>>>>>>....>>>>>>>>>...<<<.....>>>......>>>>>>..
....>.>>..<<<<<<....<<<<<....>>>>>.<<<<<<<<...<<<<
<<<<<.....<<<...<<<.<<<<....>>>>.>>>..>>.>.....>>>
>>>.>>>...>>>>>>>.>...<<<<<<<<<<......>>>>>>>>>>..
..<<<......>>>...<<<<.<<<...<<<(((.....<<<<<<.....
......<<..<<<<<..<<........<..>....>>..>>>>>.>>...
........))).....>>>>>>.>>>...>>>>>>><<<<<<<..>>>>>
>>....>>>>>>..<<<<<...<<<......<<<<<<<<....>>>>>>>
>...>>>..>>>>>........>>>>>><......<<.....<<<<<<..
.....>>>>>><<<<....<<<<<.<<...<<<<<<...<<.....>>.>
>>>>>....((<<<.<<<...<<<...<<<....>>>...>>>...>>>.
.<<<<<........>>>>>))<<<<<<<<<<<<<...<<<<....<<<<.
.<<<..<<<<<<<<<<<....(((...>>>>>><<..<<<<...<<<<<<
<<<<<<.<<.....>>>>>>>>>>..>>>>...>>>>...>><..<<<<<
....>>>>>...>>>>>>...>>>...>>>>.........>>>>...>>.
...>>>>>>>>>>>.>>>..>>.....<<......>>>>>>>.<<<<...
..>>>>..>>>>..<<<<....<<<<<<<<.<<...............<<
<<<...............>>>>><<<<<<.....<<<...<<<<.....>
>>>...>>>...>>>>>>............<<<<<<<<<..<<<......
...>>>..<<<<<<<.....<<<....>>>.......>>>>>>>.<<<<<
.<<<..<<<<<<<..<<<<...<...<<<<<..<<....>>...>>>>>.
.>....>>>>>>>>>..>>>>>........<...........<<<<<<..
...>>>>>>.....>....>>>>>..>>.>>>>>>>.....>>.>>>>>>
>>.>>>>>>.....>.....<<<<<........>>>>>..<<<<<<<<.(
((.[[......<<<<.[[<<<<..<<<<<.<<<.<<<<<.<<<<..>>>>
...<<<.<<<<<<.....>>>>>>>>>....>>>>>.>>>..>>>>>..<
<<<.....>>>><<<<<<...........>>>>>>.(<<...<<<<<<<.
..........<<)..(((>>....>>>>>>>...<<<<..<<<<....>>
>>..>>>>.<<<....>>>.>>))).......<<<<<<<.((..>>>>..
))>>>>>>>.............>>>>..]]>>>>.......<<<<...<<
<<<<...<<.......>>...>>>>>>....>>>.>.......<<<<<.<
<<<<<..<<<<<<<<<......>>>>>>>..>>.<<<<<.....>>>>>.
....>>>>>..>...>>>.>>....<<<<<<<....>>>.>>>>....]]
..)))>>>>>>>>....<<<<<<<...<<<<..<<<<<<<..........
.....>>>>>>>.<<<<<...<<<<...<<<<<<<<<<<))).>>>>>>.
.>>>>>...>>>>.>>>>>...<<<....<<<<<...........>>>>>
....>>>.>>>>.......>>>>>>>..<<<<<.....>>>>><....<<
..<<<<<.......>>>>>....<<<<<<<<..<<....<<<<<....>>
>>>...>>...>>>>>.>>>.....>>..>..>>>>>>>>
Receptor-Ligand Complex Structure
Global viewLocal viewStructure summary

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PDB8cam Structural conservation of antibiotic interaction with ribosomes.
Resolution1.86 Å
Binding residue
(original residue number in PDB)		P4 K5 R6 K8 F9 K11 M12 H13 K14 G15 R16 N17 Q22 G23 F28 R44 Q45 A48 R55 K62 W64 R66 F68 D70 E75 K76 L78 A79 X81 X82 G83 K84 G85 K86 K100 A122 K123 P125 K127
Binding residue
(residue number reindexed from 1)		P4 K5 R6 K8 F9 K11 M12 H13 K14 G15 R16 N17 Q22 G23 F28 R44 Q45 A48 R55 K62 W64 R66 F68 D70 E75 K76 L78 A79 X81 X82 G83 K84 G85 K86 K100 A122 K123 P125 K127
Enzymatic activity
Enzyme Commision number ?
Gene Ontology
Molecular Function
GO:0000049 tRNA binding
GO:0003735 structural constituent of ribosome
GO:0019843 rRNA binding
Biological Process
GO:0000027 ribosomal large subunit assembly
GO:0002181 cytoplasmic translation
GO:0006412 translation
Cellular Component
GO:0005737 cytoplasm
GO:0005840 ribosome
GO:0022625 cytosolic large ribosomal subunit
GO:1990904 ribonucleoprotein complex

View graph for
Molecular Function
View graph for
Biological Process
View graph for
Cellular Component
External links
PDB RCSB:8cam, PDBe:8cam, PDBj:8cam
PDBsum8cam
PubMed37550453
UniProtP0ADY7|RL16_ECOLI Large ribosomal subunit protein uL16 (Gene Name=rplP)

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