Structure of PDB 8q4f Chain k Binding Site BS01

Receptor Information

>8q4f Chain k (length=144) Species: 562 (Escherichia coli)

MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGGGVRRGF
EGGQMPLYRRLPKFGFTSRKAAITAEIRLSDLAKVEGGVVDLNTLKAANI
IGIQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE

Ligand information

>8q4f Chain a (length=2732)

gguuaagcgacuaagcguacacgguggaugcccuggcagucagaggcgau
gaaggacgugcuaaucugcgauaagcgucgguaaggugauaugaaccguu
auaaccggcgauuuccgaauggggaaacccaguguguuucgacacacuau
cauuaacugaauccauagguuaaugaggcgaaccgggggaacugaaacau
cuaaguaccccgaggaaaagaaaucaaccgagauucccccaguagcggcg
agcgaacggggagcagcccagagccugaaucaguguguguguuaguggaa
gcgucuggaaaggcgcgcgauacagggugacagccccguacacaaaaaug
cacaugcugugagcucgaugaguagggcgggacacgugguauccugucug
aauauggggggaccauccuccaaggcuaaauacuccugacugaccgauag
ugaaccaguaccgugagggaaaggcgaaaagaaccccggcgaggggagug
aaaaagaaccugaaaccguguacguacaagcagugggagcacgcuuaggc
gugugacugcguaccuuuuguauaaugggucagcgacuuauauucuguag
caagguuaaccgaauaggggagccgaagggaaaccgagucuuaacugggc
guuaaguugcaggguauagacccgaaacccggugaucuagccaugggcag
guugaagguuggguaacacuaacuggaggaccgaaccgacuaaugaaaaa
uuagcggaugacuuguggcugggggugaaaggccaaucaaaccgggagau
agcugguucuccccgaaagcuauuuagguagcgccucgugaauucaucuc
cggggguagagcacuguuucggcaagggggucaucccgacuuaccaaccc
gaugcaaacugcgaauaccggagaauguuaucacgggagacacacggcgg
ggcuaacguccgucgugaagagggaaacaacccagaccgccagcuaaggu
cccaaagucaugguuaagugggaaacgaugugggaaggcccagacagcgu
cgagucggccugcgcggaagauguaacggggcuaaaccaugcaccgaagc
ugcggcagcgacgcgcguuguuggguaggggagcguucuguaagccugcg
aaggugugcugugaggcaugcuggagguaucagaagugcgaaugcugaca
uaaguaacgauaaagcgggugaaaagcccgcucgccggaagaccaagggu
uccuguccaacguuaaucggggcagggugagucgaccccuaaggcgaggc
cgaaaggcguagucgaugggaaacagguuaauauuccuguacuugguguu
acugcgaaggggggacggagaaggcuauguuggccgggcgacgguugucc
cgguuuaagcguguaggcugguuuuccaggcaaauccggaaaaucaaggc
ugaggcgugaugacgaggcacuacggugcugaagcaacaaaugcccugcu
uccaggaaaagccucuaagcaucagguaacaucaaaucguaccccaaacc
gacacgguggucagguagagaauaccaaggcgcuugagagaacucgggug
aaggaacuaggcaaaauggugccguaacuucgggagaaggcacgcugaua
uguaggugaggucccucgcggauggagcugaaaucagucgaagauaccag
cuggcugcaacuguuuauuaaaaacacagcacugugcaaacacgaaagug
gacguauacggugugacgccucccggugccggaagguuaauugauggggu
uagcgcaagcgaagcucuugaucgaagccccgguaaacggcggccguaac
uauaacgguccuaagguagcgaaauccuugucggguaaguuccgacugca
cgaauggcguaaugauggccaggcugucuccacccgagacucagugaaau
ugaacucgcugugagaugcaguguacccgcggcaagacggaaagaccccg
uaaccuuuacuauagcuugacacugaacauauguucuaacguugacccgu
aauccggguugcggacagugucugguggguaguuugacugggcggucucc
uccuaaagaguaacggaggagcacgaagguuggcuaauccuggucggaca
ucaggagguuagugcaauggcauaagccagcuugacugcgagcgugacgg
cgcgagcaggugcgaaagcaggucauagugauccggugguucugaaugga
agggccaucgcucaacggauaaaagguacuccgggaaacaggcgauaccg
cccaagaguucauaucgacggcgguguuuggcacucggucggcucaucac
auccuggggcugaaguaggucccaaggguauggcguucgccauuuaaagu
gguacgcgagcggguuuagaacgucgugagacagcggucccuaucugccg
ugggcgcuggagaacugaggggggcugcuccuaguacgagaggaccggag
uggacgcaucacugguguucggguugucaugccaauggcacugcccggua
gcuaaaugcggaagagauaagugcugaaagcaucuaagcacgaaacuugc
cccgagaugaguucucccugacccuuuaaggguccugaaggaacguugaa
gacgacgacguugauaggccggguguguaagcgcagcgaugcguugagcu
aaccgguacuaaugaaccgugaggcuuaaccu

..<<<<<<......<<<<<<<<<<<.....<<..<<<<<<<<<<<<<...
...<<<<<<.[..(((.>>>.....<<<<<<<..<<<)))..].>>>...
...>>>>>>>...>>>....<<<....>>><<<<<<<....>>>>>>>.<
<<<<<<<<.........>>>>>>>>>.>>...<<<<<<.....<<....>
>......>>>>>.>.........<<....>>..<<<<<<.....<<....
.>>....>>>>>>..<<<..<<<<......<<<<<<<<<<<<<.<<<...
.<<<<......>>>>.((......<<<.....))>>>...>>>...>>>>
>>>>>>>>>...>>>>........<<<<<<<<.........>>>>>>>>.
.....<<<<<..((((>>>>>..>>>......>>.>>>>>>>.>>.....
..........<<....>>....>>.........<<<<.....>>>>....
..............>>>>>>>>>>>.......<<<<..<<<<<<<..>>>
>>>>..>>>>...<.<........>.>.<<<<<<....<<<<<<<<<<<<
<..<<<...<<.....>>...>>>...<<....>>..<<<<.....>>>>
.....>>>>>>>>>>>>>..<<.<..<<<<<<<.....<<<<<<<<<..<
<<....<<<<<<......>>>>>>.....>>>...<<<.<<<<<.....>
>>>>>>>....>>>>>>>>>...<<<.....>>>......>>>>>>...>
..>.>>..<<<<<<....<<<<<....>>>>>.<<<<<<<<...<<<<<<
<<<.....<<<...<<<.<<<<...<<.<..<.....>..>..>>...>>
>>.>>>..>>.>.....>>>>>>.>>>...>>>>>>>.>...<<<<<<<<
<.......>>>>>>>>>....<<<......>>>...<<<<.<<<...<<<
(((.....<<<<<<...........<<..<<<<<..<<........<..>
....>>..>>>>>.>>...........))).....>>>>>>.>>>...>>
>>>>><<<<<<<<..>>>>>>>>....>>>>>>..<<<<<...<<<....
..<<<<<<<<....>>>>>>>>...>>>..>>>>>........>>>>>><
......<<.....<<<<<<.......>>>>>><<<<....<<<<<.<<..
.<<<<<<...<<.....>>.>>>>>>....((<<<.<<<.<.<<<...<<
<....>>>...>>>..>>>>..<<<<<........>>>>>))<<<<<<<<
<<<<<...<<<<....<<<<..<<<..<<<<<<<<<<<....[[[...>>
>>>><<..<<<<...<<<<<<<<<<<<.<<.....>>>>>>>>>>..>>>
>...>>>>...>><..<<<<<<..>>>>>>...>>>>>>...>>>...>>
>.>........>>>>...>>....>>>>>>>>>>>.>>>..>>.....<<
.....>>>>>>>.<<<<.....>>>>..>>>>..<<<<....<<<<<<<<
.<<.........<.....<<<<<.....<..>......>>>>><<<<<<.
....<<<...<<<<.....>>>>...>>>...>>>>>>............
<<<<<<<<<..<<<.........>>>..<<<<<<<.....<<<....>>>
.......>>>>>>>.<<<<<.<<..<<<<<<<..<<<<.......<<<<<
<.<<....>>..>>>>>>.......>>>>>>>>>..>>>>.<<<<<<...
....>>>>>>........<..........<<<<<.......>>>>>....
>....>>>>>..>>.>>>>>>>..>..>>.>>>>>>>>.>>>>>>.....
>.....<<<<<.......>>>>>..<<<<<<<<.<<<.<<.......<<<
..<<<<..<<<<<.<<<.<<<<<.<<<<<..>>>>>...<<<.<<<<<<.
....>>>>>>>>>....>>>>>.>>>..>>>>>..<<<......>>><<<
<<<...........>>>>>>..<<...<<<<<<<...<<<<<<.......
>>>>>>.....<<...(((>>....>>>>>>>...<<<<..<<<<....>
>>>..>>>>.<<<....>>>.>>))).......<<<<<<<.<<..)))).
.>>>>>>>>>.............>>>>....>>>.......<<.<.<<<<
<<...<<.......>>...>>>>>>...>.>>......<<.<<..<<<<<
..<<<<<<<<<......>>>>>>>..>>.<<<<<....>>>>>.....>>
>>>......>>.>>....<...<<<....>>>..>....>>..>>>>>>>
>>>>....<<<<<<<...<<<<..<<<<<<<...............>>>>
>>>.<<<<<...<<<<...<<<<<<<<<<<]]].>>>>>>..>>>>>...
>>>>.>>>>>...<<<.<..<<<<.............>>>>...>>>>.>
>>>.......>>>>>>>..<<<<<.....>>>>><....<<..<<<<<..
.....>>>>>....<<<<<<<<..<<....<<<<<....>>>>>...>>.
..>>>>>.>>>.....>>..>..>>>>>>...

Receptor-Ligand Complex Structure

Structure summary

• PDB: 8q4f Structure of arbekacin bound Escherichia coli 70S ribosome
• Resolution: 3.1 Å
• Binding residue (original residue number in PDB): N4 S7 E10 G11 K13 K14 G16 K17 R18 L19 G20 R21 G22 I23 G24 K29 T30 G32 R33 G34 H35 K36 Q38 K39 R41 S42 G43 R48 F50 E51 G52 G53 Q54 M55 Y58 R59 R60 P62 K63 F64 G65 F66 S68 R69 K70 E76 R78 N99 I103 Q104 K109 I111 L112 T128
• Binding residue (residue number reindexed from 1): N4 S7 E10 G11 K13 K14 G16 K17 R18 L19 G20 R21 G22 I23 G24 K29 T30 G32 R33 G34 H35 K36 Q38 K39 R41 S42 G43 R48 F50 E51 G52 G53 Q54 M55 Y58 R59 R60 P62 K63 F64 G65 F66 S68 R69 K70 E76 R78 N99 I103 Q104 K109 I111 L112 T128

External links

• PDB: RCSB:8q4f, PDBe:8q4f, PDBj:8q4f
• PDBsum: 8q4f
• UniProt: P02413|RL15_ECOLI Large ribosomal subunit protein uL15 (Gene Name=rplO)