Structure of PDB 5t5h Chain k Binding Site BS01

Receptor Information

>5t5h Chain k (length=113) Species: 5693 (Trypanosoma cruzi)

IRDLRDKSKDELLKQLMEFKKELSQLRVAQQLGRIRLIRKSIARILTVLN
RNQRESLRKVYAGRRLRLAMPKALRPKLTRRRRLALKDNEKNRKTRRQLR
MARKFPRRVYAVK

Ligand information

>5t5h Chain A (length=1278)

cagaccugaguguggcaggacuacccgccgaacuuaagcauauuacuaag
cggaggaaaagaaaacaaccgugauucucucagucagcggcgagcgaaga
gggauccaacucguugccgaaucgguucuuauaggaacuugaguuguggc
aauggugcaggaauggugaaauucaaagcaaacccguugcuacaucaugu
gaguauugagccaaagaagguguuagcccauugagccaugaaccuaagag
aguagcacugcuugggagugcagugucaagcuggcagguaugugucugcc
aaaguuaaauacagaguaggaagaccgauagcgaacaaguagcgugagcg
aaaguuugaaaagcacuuuggaaagagagugacauagaccugaagucgug
acaauaauggaaguaccuccauuucgccaaaaccagccucuacugagcaa
gaacgaggucaugguuuuggcggaaauggggugcccccacccgucuugaa
acacggaccaaggagucaaacagacgcgcaagggagaagauaucaguacu
ucucucguacugaaagggagauuguggugugcacacuugcccaacaccga
ccggccccggguugaguuggagugugccuguuuggacccgaaagguggug
aacuaugccugaacaaggugaagcccggggaaacucggguggaggccugu
agcgaugcugacgugcaaaucgcucgcaugauuuggguaucggagcgaaa
gacucaucgaaccaccuaguagcugguucacaucgaaguuucccucagga
uagcuggugcuugaaaaaguauugugcgguaaagcuuuaaaugugcaacu
ugccaaagcaagguauuaaggcaccaagugggccuucuucgguaagcgga
gcaggcgaugcggaaugaaccgcuuagggauauguggcgucaaaacuuau
gggcucacgaacaauggaugacgcucaagcccaauuagugcccauuauuc
cuuugguaagggcgagaaaguguagcccuuccgauguagauguggcgugg
agguaaggacgaagcugauggcgugagccucagauggaccagccucuagu
gcagaucuugguuggcguagcaaagaucuaacggaacguuggauacugga
gcggggaaggauuucgugccaacggcacucguacacgaguuguucggaua
cugagcacaacguuacaccguuuuuaggaaaaggggcaacagagaaccug
ggauuauauuccaaaaagaaauugcaug

.......................<<<<<......<......>.......>
>>>.>.........<<....>>...<<<<<......<<.....>>....>
>>>>....<<<..<<<<<<..<<<<<<<......>>>>>.>>....>>>>
>>......<<<.<<<<<......<<<<<<<.......>>>>.<.....>.
.....>>>.<<.......((.....>>)).....>>>>>...>>>.....
....<<<<<<<........>>>>>>>......<<<<<........>>>>>
..>>>....................................<<....>>.
...............<<<......>>>.......................
.......<<<..<<<<<<.<<<<<.<<<<<<<<<<<<<<...<<.>>...
.....>>>>..>>>>>>>>>>..>>>>>>>>>>>..>>>.<<<<......
..>>>><<<<<<...<<<<<<<<.<<<<<..<<<<..<<.<<<..>>>>>
..>>>>...<<....>>........<<<<<...<<..>>....>>>>><<
<<<<<<<.>>>>>>.>>>...>>>>>.>>>>>>>>.<<.<...<<<<<<.
....<<<<<<<<<..<<<....<<<<<<......>>>>>>.....>>>..
.<<<<.<<.<<........>>>>>>>>....>>>>>>>>>...<<.....
..>>......>>>>>>......>.>>...<<<<<....<.<<<....>>>
(><<<<<<<<<.....<<...<<<<<<<...<<<.>>>...>>>>>>><<
<<<....>>>>>...>>..>>>>>>>.>>).<<<<.<<<<<.....>>>>
>.>>>>...<<<<......>>>>..<<<<<<<...<<<(((......<..
<<<<....<..........))).>....>>>>.>.....>>>...>>>>>
>><<<<.<<<<<.............>>>>>>>>>....>>>>>.....<<
<<<<..........<<<..<<<....>>>.>>>........>>>>>>...
......>>>>>>..............<<<<<<<...>>>>>>>.......
.............<<<<<....<<.....>>..>>>>>....<<......
....<<.<<<<<......>>>>...........>.>>..........<<<
<.........>>....>>.>>.......

Receptor-Ligand Complex Structure

Structure summary

• PDB: 5t5h Structure and assembly model for the Trypanosoma cruzi 60S ribosomal subunit.
• Resolution: 2.54 Å
• Binding residue (original residue number in PDB): R77 R78 R93 L97 R106 K107 R109 R110 Q111
• Binding residue (residue number reindexed from 1): R64 R65 R80 L84 R93 K94 R96 R97 Q98

Gene Ontology

Molecular Function

• GO:0003729 mRNA binding
• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0000463 maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
• GO:0006412 translation

Cellular Component

• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:5t5h, PDBe:5t5h, PDBj:5t5h
• PDBsum: 5t5h
• PubMed: 27791004
• UniProt: Q4E2E0