Structure of PDB 7ls1 Chain j2 Binding Site BS01

Receptor Information

>7ls1 Chain j2 (length=89) Species: 10090 (Mus musculus)

RTKKVGIVGKYGTRYGASLRKMVKKIEISQHAKYTCSFCGKTKMKRRAVG
IWHCGSCMKTVAGGAWTYNTTSAVTVKSAIRRLKELKDQ

Ligand information

>7ls1 Chain A2 (length=3707)

cgcgaccucagaucagacguggcgacccgcugaauuuaagcauauuaguc
agcggaggaaaagaaacuaaccaggauucccucaguaacggcgagugaac
agggaagagcccagcgccgaauccccgccgcgcgucgcggcgugggaaau
guggcguacggaagacccacuccccggcgccgcucguggggggcccaagu
ccuucugaucgaggcccagcccguggacggugugaggccgguagcggccc
ccggcgcgccgggcucgggucuucccggagucggguugcuugggaaugca
gcccaaagcgggugguaaacuccaucuaaggcuaaauaccggcacgagac
cgauagucaacaaguaccguaagggaaaguugaaaagaacuuugaagaga
gaguucaagagggcgugaaaccguuaagagguaaacgggugggguccgcg
caguccgcccggaggauucaacccggcggcgcguccggccgugccggugg
uccggcggaucuuucccgcgcgggggaccgcccccggccggcgaccggcc
gccgccgggcgcacuuccaccguggcggugcgccgcgaccggcuccggga
cggccgggaaggcccgguggggaagguggcucggggggggcucaccccga
guguuacagcccuccggccgcgcuuuucgccgaaucccggggccgaggaa
gcccgucgccgcgcucucccucucccgggugggggccgggccgccccucc
cacggcgcgaccgcucggggcggacuguccccagugcgccccgggcgucg
ucgcgccgucgggucccgggggcgucggucacgcgugacgaagccgagcg
cacggggucggcggcgaugucggcuacccacccgacccgucuugaaacac
ggaccaaggagucuaacgcgugcgcgagucaggggcucguccgaaagccg
ccguggcgcaaugaaggugaagggccccgcccgggggcccgaggugggau
cccgaggccucuccaguccgccgagggcgcaccaccggcccgucucgccc
gccgcgccggggagguggagcacgagcguacgcguuaggacccgaaagau
ggugaacuaugcuugggcagggcgaagccagaggaaacucugguggaggu
ccguagcgguccugacgugcaaaucggucguccgaccuggguauaggggc
gaaagacuaaucgaaccaucuaguagcugguucccuccgaaguuucccuc
aggauagcuggcgcucucgcuacgcaguuuuauccgguaaagcgaaugau
uagaggucuuggggccgaaacgaucucaaccuauucucaaacuuuaaaug
gguaagaagcccggcucgcuggcguggagccgggcguggaaugcgagugc
cuagugggccacuuuugguaagcagaacuggcgcugcgggaugaaccgaa
cgccggguuaaggcgcccgaugccgacgcucaucagaccccagaaaaggu
guugguugauauagacagcaggacgguggccauggaagucggaauccgcu
aaggaguguguaacaacucaccugccgaaucaacuagcccugaaaaugga
uggcgcuggagcgucgggcccauacccggccgucgccggcagucggaacg
gaacgggacgggagcggcgucgggccccgcggagccuacgccgcgacgag
uaggagggccgcugcggugagccuugaagccuagggcgcgggcccgggug
gagccgccgcaggugcagaucuuggugguaguagcaaauauucaaacgag
aacuuugaaggccgaaguggagaaggguuccaugugaacagcaguugaac
augggucagucgguccugagagaugggcgagugccguuccgaagggacgg
gcgauggccuccguugcccucggccgaucgaaagggagucggguucagau
ccccgaauccggaguggcggagaugggcgccgcgaggcguccagugcggu
aacgcgaccgaucccggagaagccggcgggagccucggggagaguucucu
uuucuuugugaagggcagggcgcccuggaauggguucgccccgagagagg
ggcccgugccuuggaaagcgucgcgguuccggcggcguccggugagcucu
cgcuggcccuugaaaauccgggggagaggguguaaaucucgcgccgggcc
guacccauauccgcagcaggucuccaaggugaacagccucuggcauguug
gaacaauguagguaagggaagucggcaagccggauccguaacuucgggau
aaggauuggcucuaagggcugggucggucgcggccggcgccuagcagccg
acuuagaacuggugcggaccaggggaauccgacuguuuaauuaaaacaaa
gcaucgcgaaggcccgcggcggguguugacgcgaugugauuucugcccag
ugcucugaaugucaaagugaagaaauucaaugaagcgcggguaaacggcg
ggaguaacuaugacucucuuaagguagccaaaugccucgucaucuaauua
gugacgcgcaugaauggaugaacgagauucccacugucccuaccuacuau
ccagcgaaaccacagccaagggaacgggcuuggcggaaucagcggggaaa
gaagacccuguugagcuugacucuagucuggcacggugaagagacaugag
agguguagaauaagugggaggcccggccgccggugaaauaccacuacucu
caucguuuuuucacugacccggugaggcgggggggcgagccccgaggggc
ucucgcuucuggcgccaagcguccgucccgcgcgugcgggcgggcgcgac
ccgcuccggggacagugccagguggggaguuugacuggggcgguacaccu
gucaaacgguaacgcagguguccuaaggcgagcucagggaggacagaaac
cucccguggagcagaagggcaaaagcucgcuugaucuugauuuucaguac
gaauacagaccgugaaagcggggccucacgauccuucugaccuuuugggu
uuuaagcaggaggugucagaaaaguuaccacagggauaacuggcuugugg
cggccaagcguucauagcgacgucgcuuuuugauccuucgaugucggcuc
uuccuaucauugugaagcagaauucaccaagcguuggauuguucacccac
uaauagggaacgugagcuggguuuagaccgucgugagacagguuaguuuu
acccuacugaugauguguuguugccaugguaauccugcucaguacgagag
gaaccgcagguucagacauuugguguaugugcuuggcugaggagccaaug
gggcgaagcuaccaucugugggauuaugacugaacgccucuaagucagaa
ucccgcccaggcggaacgauacggcagcgccgaggagccucgguuggccc
cggauagccggguccccguccugccccgcggccgggcgucgggaccgggg
uccggugcggagagccguucgucuugggaaacggggugcggccggaaagg
gggccgcccucucgcccgucacguugacgcacguucguguggaaccuggc
gcuaaaccauucguagacgaccugcuucugggucgggguuucguacguag
cagagcagcucccucgcugcgaucuauugaaagucagcccucgacacaag
gguuugu

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>>>>.....(((((..<<<<<<)))))..]]]..................
................<<....>>...............<<<......>>
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>>>>>>>>>........>.>>>.>>>>>...<<<<<<<.<<<<<<<<<<.
<<.....<<.<<<<.<<.<.<<......<<<<<<<<.<<..>>.>>>>>>
>>........>>>>>>>>>.......>>..>>...>>>>>>>>>>.....
..........<<<<<<.<<<<<.<<..>>.>>>>>...<<<..<<<<.<<
<<<<<<<<<<<<<<<<<<<<<<<...........>.>>>>>>>>>>>..>
>>>>>>>>>.>>>....>>>>..>>>.....<<.....>>.....>>>>>
>......>>.>>>>>...>>>>>...>>>>>>>>..<<<<........>>
>><<<<<<....<<<<<<<<<<<<<..<<<...<<<<........>>>>.
....>>>...<<....>>...<<<<<<<.....>>>>>>>..<<<<<...
<<<..<<<...(((.[[..>>>..>>>....>>>>>.<<<<<<<<<.<<<
<......>>>>>>>>>>>.>>....>>>>>>>>>>>>>..<<.<..<<<<
<<<.....<<<<<<<<<..<<<....<<<<<<<....>>>>>>>.....>
>>...<<<..<..<<........>>.>.>>>....>>>>>>>>>...<<.
......>>......>>>>>>...>..>.>>..<<<<<<....<.<<<...
.>>>.><<<<<<<<<<.......<<<.<<<<<<<<<.<...<....<<<.
...<<<<.....<<..........>>..>>>>....>>>..>.....>>>
>>>>>...<<<<<<<<<.<......>>>>>>>>>>....>>>>>>>>>>>
>.>>>..<<<<.<<<<<.....>>>>>.>>>>....<<<......>>>..
.<<<<<<<...<<<(((.......<<<<<.....<<..........<<<.
<<<<<<<<<<.......<<<<<.<<....<<.........>>....>>..
.......<<......>>...>>>>>...>>>>>>>>>>>>>.........
.))).>>...>>>>>..>>>...>>>>>>><<<<<<.<<<..........
..]].)))...<<<...>.>>..................>>>>>>>>>..
..>>>>>>.<.<<<<<<<..<<......<<<<.<<<<....>>>>>>>>.
..>>.>>>>>>>..>.....>>>>>><<<....<<.....<<<<<<....
...>>>>>>.<<<.....<<<<<.<<...<<<<<<...<<.....>>.>>
>>>>....((<<<<<<...<<....<<<....>>>..........<<.<<
<<<.<<<...>>>.>>>>>>>......>>....>>...<<<<<.......
.>>>>>))<<<<....<<<<<...<<<<<<<....>>>>>>>...<<<..
..>>>...<..<<<<<<<...<<<<<<<<<<<<..<<<<<<<<<..{{{.
.>>>>>>....<<<<<.<<<<.<<<......<<<.....>>>......>>
>>>>>..>>>>>.....<<<<<<<.......>>>>>>>>>>....>>>>>
>>>>>>>.........>>>>>>>..>..........>>.>>>.>>>>>>>
>..>>.....<<......>>>>>>>.<<<<.....>>>>..>>>....<<
<<.....<<<<<<<.<<.........<.....<<<<<....<....>...
..>>>>><<.<<<<<<<<<<<...<..........>........>>>>>.
.>>>>>>.>>............<<<<<<<<<..<<<.........>>>..
<<<<<<<....<<<<.....>>>>......>>>>>>>....<<.<<<..<
<<<<<....<<<.....<<<<....>>>>.....>>>.>>>>..>>>>>.
<<<<<.......>>>>>.........<...........<<<<<.......
>>>>>.....>.....>>....>>.>>>>>>>..>..>>.>>>>>>>..>
>>>>>...>>>....<<<<<........>>>>>....[[[[[..{{{.{{
......[[[[.{{[[[[..<<<<<.<<<<<<<<<.<<<<<<<<<<<<..<
<<...........<..............................>.>>>.
.>>.>>>>>>>>>>...<<<<<.....<.<<<....<<<<.<<...>>>>
>>................<<<<<<<.<<<<<....>>>>>>>>>>>>..>
>>>..>>>>>....>>>>>>>>>..>>>>>...<<<.....>>>..<<<<
<<...........>>>>>>..<<....<<<<<<...<<<<<<.......>
>>>>>.....<<...(((>>....>>>>>>....<<<..<<<<.......
>>>>..>>>.<<<....>>>.>>))).......((((((..(((..>>>>
>>.)))))))))..........]]]]..}}]]]].......<<.<.<.<<
<<<<...<<.......>>...>>>>>>...>>..>>.......<<<<<.<
<<<<<..<.<.<<<<<......>>>>>...>.>.<<<.........>>>.
....>>>>>..>...>>>.>>....<<<<<<<....>>>.>>>>....}}
..}}}.]]]]].....<<<<<<..<.<<<..<.<<<<<............
.....>>>>>.><<<<....<<<<....<<.<<<<<<<}}}.>>>>>..>
>.>>...>>>>...>>>><<<<....<<<<<...........>>>>>...
.>>>>.>>>...>.>>>>>>.....<<<<<<.<<<..<<.<....<<<..
......<<<<<<.<<<<<..<<<.....<<<>>>.....>>.>..>>>>>
>>>>>>.......>>>...<<<..<<.<....<<<<.<<<<<<.......
.>>>>>>>>>>....>>>..>>>...............>.>>..>>>>>>
>>><<<<<.<.............>....<<.<<<<<<<.<<.....<<<<
....<<<<<......>>>>>...>>>>....>>....>>>>.>>>.>>..
>>>>>..

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7ls1 Functionally distinct roles for eEF2K in the control of ribosome availability and p-body abundance.
• Resolution: 3.3 Å
• Binding residue (original residue number in PDB): R4 T5 K6 V8 G9 I10 K13 Y14 G15 T16 R17 G19 A20 S21 R23 H34 F41 C42 K44 K46 R49 R50 A51 V52 G58 S59 M61 K62 W69
• Binding residue (residue number reindexed from 1): R1 T2 K3 V5 G6 I7 K10 Y11 G12 T13 R14 G16 A17 S18 R20 H31 F38 C39 K41 K43 R46 R47 A48 V49 G55 S56 M58 K59 W66

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome
• GO:0046872 metal ion binding

Biological Process

• GO:0002181 cytoplasmic translation
• GO:0006412 translation
• GO:0140236 translation at presynapse
• GO:0140242 translation at postsynapse

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:0045202 synapse
• GO:0098793 presynapse
• GO:0098794 postsynapse
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:7ls1, PDBe:7ls1, PDBj:7ls1
• PDBsum: 7ls1
• PubMed: 34815424
• UniProt: P61514|RL37A_MOUSE Large ribosomal subunit protein eL43 (Gene Name=Rpl37a)