Structure of PDB 8wif Chain j Binding Site BS01

Receptor Information
>8wif Chain j (length=126) Species: 246196 (Mycolicibacterium smegmatis MC2 155) [Search protein sequence] [Download receptor structure] [Download structure with residue number starting from 1] [View receptor structure]
PIQTVGRRKEAVVRVRLVPGTGQFNLDGRTLENYFPNKVHQQLIKAPLVT
VDRVDQFDIYAHLDGGGPSGQAGALRLAIARALILVQPEDRPALKKAGFL
TRDPRAIERKKYGLKKARKAPQYSKR
Ligand information
>8wif Chain a (length=1517) [Search RNA sequence] [Download ligand structure] [Download structure with residue number starting from 1] [View ligand structure]
uuuggagaguuugauccuggcucaggacgaacgcuggcggcgugcuuaac
acaugcaagucgaacggaaaggcccuuucggggguacucgaguggcgaac
gggugaguaacacgugggugaucugcccugcacuuugggauaagccuggg
aaacugggucuaauaccgaauacacccugcuggucgcauggccugguagg
ggaaagcuuuugcggugugggaugggcccgcggccuaucagcuuguuggu
ggggugauggccuaccaaggcgacgacggguagccggccugagaggguga
ccggccacacugggacugagauacggcccagacuccuacgggaggcagca
guggggaauauugcacaaugggcgcaagccugaugcagcgacgccgcgug
agggaugacggccuucggguuguaaaccucuuucagcacagacgaagcgc
aagugacgguaugugcagaagaaggaccggccaacuacgugccagcagcc
gcgguaauacguaggguccgagcguuguccggaauuacugggcguaaaga
gcucguaggugguuugucgcguuguucgugaaaacucacagcuuaacugu
gggcgugcgggcgauacgggcagacuagaguacugcaggggagacuggaa
uuccugguguagcgguggaaugcgcagauaucaggaggaacaccgguggc
gaaggcgggucucugggcaguaacugacgcugaggagcgaaagcgugggg
agcgaacaggauuagauacccugguaguccacgccguaaacgguggguac
uagguguggguuuccuuccuugggauccgugccguagcuaacgcauuaag
uaccccgccuggggaguacggccgcaaggcuaaaacucaaaggaauugac
gggggcccgcacaagcggcggagcauguggauuaauucgaugcaacgcga
agaaccuuaccuggguuugacaugcacaggacgccggcagagaugucggu
ucccuuguggccugugugcagguggugcauggcugucgucagcucguguc
gugagauguuggguuaagucccgcaacgagcgcaacccuugucucauguu
gccagcacguuaugguggggacucgugagagacugccggggucaacucgg
aggaagguggggaugacgucaagucaucaugccccuuauguccagggcuu
cacacaugcuacaauggccgguacaaagggcugcgaugccgugaggugga
gcgaauccuuucaaagccggucucaguucggaucggggucugcaacucga
ccccgugaagucggagucgcuaguaaucgcagaucagcaacgcugcggug
aauacguucccgggccuuguacacaccgcccgucacgucaugaaagucgg
uaacacccgaagccgguggccuaacccuuguggagggagccgucgaaggu
gggaucggcgauugggacgaagucguaacaagguagccguaccggaaggu
gcggcuggaucaccucc
......<<<<..[.((((.>>>>.<<<<.<<<<<..<<<<<<<<.....<
<<.<<<..<<<..<<.<..<<<<<<<....>>>>>.>>>..>>>>>....
..<<........<<<<<<<..<<...<<<<<<<<<<<<.....<<<<<<.
...>>>>>>......>>>>.....<<<<<<<<<<<<..>>>>>.>>>>>>
>....<<....>>>>>>>>>>..>>>>>>>>>.<<<....<<<..<<<<<
<<<.......>>>>>>>>>>>......>>>..<<<<<<<<....>>>>..
.>>>>.>>.<<<<<.<.........>>>>>>.<<<<....>>>>...>>>
>>>.........<<<...<<<<<....>>>>.>>>>..>>.>>>>>>..<
<<<......<<<<....>>>>.....>>>>...<.<<<<<......<<..
..>>.......>>>>>....>..<<<<<(((...<<<<<.....<<.)))
>>.......>>>>>>>>>>..>>>>>>>>>..........<<<((.....
<<<<...<<<.<<<<<<<.<<<<<<<<<<.....<<<<<<<.....>>>>
>>>...>>>>>>>..>>>>>>>>>>...<<<<<<<<...<<<<<<<....
<<<<<<<<<..<<<......>>>.....>>>>>>>>>...........<<
....>>.>>>>>>>..>>>>>.>>>...>>>...>>>>....<<<<<<..
.<<...<<<<.<.....>.>>>>...>>>>>>>>..........<<<<<<
..<<<<<<<<<<<<.......>>>>>>>>>>>>...<<..))>>.....>
>>>>>.>>>.<<<......<<<<....>>>>....>>>..)))).]<<<<
<.<<<<<<<.<<.<<<<<<..<<<<<<<<<<......<<........>>.
.........<<<<<<<......<<<<<<<...<<<<<<<....>>>>>>>
.<<.....>>.>>>>>>>.<<<.<<<..<<<<<<<......<<<<<<<<<
....>>>..<<<<......>>>>..>>>>>>.....<<<<.<<<<<<...
.<<..<<<......>>>>>.....>>>>>>.....<<<<<.....>>>>>
........>>>>.......>.>>>...>>>>>>>>>...>>>>>>>...>
>.>>>>>>>>.....<<<<<<<.....<<<..<<..<<<<....>>>>..
>>....>>>......>>>>>>>......<....<<<<<<<........>>
>>>>>....>.....>>>>>>....<<<<<<<..........>>>>>>>.
.....>>...>>>>>>>>>>.>>....<..<<.<.<<<<.<<<..<<<<<
<..<<<<....<.<<<<..<<<..<<.....>>.>>>.>>>>.>...>>>
>..>>>>>>..>>>.>>>>..>.>>...>.....<<<<<<<<<....>>>
>>>>>>...........
Receptor-Ligand Complex Structure
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PDB8wif Cryo- EM structure of the mycobacterial 70S ribosome in complex with ribosome hibernation promotion factor RafH.
Resolution2.9 Å
Binding residue
(original residue number in PDB)
Q27 V29 R31 R32 K33 E34 R38 R40 H86 G89 G90 G91 S93 Q95 R115 K119 T125 R126 R129 A130 I131 E132 R133 K134 K135 Y136 G137 K139 K140 R142 K143 A144 Q146 Y147 S148
Binding residue
(residue number reindexed from 1)
Q3 V5 R7 R8 K9 E10 R14 R16 H62 G65 G66 G67 S69 Q71 R91 K95 T101 R102 R105 A106 I107 E108 R109 K110 K111 Y112 G113 K115 K116 R118 K119 A120 Q122 Y123 S124
Enzymatic activity
Enzyme Commision number ?
Gene Ontology
Molecular Function
GO:0003723 RNA binding
GO:0003735 structural constituent of ribosome
Biological Process
GO:0006412 translation
Cellular Component
GO:0005840 ribosome
GO:1990904 ribonucleoprotein complex

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Molecular Function

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Biological Process

View graph for
Cellular Component
External links
PDB RCSB:8wif, PDBe:8wif, PDBj:8wif
PDBsum8wif
PubMed38245551
UniProtA0QSP9|RS9_MYCS2 Small ribosomal subunit protein uS9 (Gene Name=rpsI)

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