Structure of PDB 7syr Chain j Binding Site BS01

Receptor Information

>7syr Chain j (length=182) Species: 9606 (Homo sapiens)

LSCRFYQHKFPEVEDVVMVNVRSIAEMGAYVSLLEYNNIEGMILLSELSR
RRIRSINKLIRIGRNECVVVIRVDKEKGYIDLSKRRVSPEEAIKCEDKFT
KSKTVYSILRHVAEVLEYTKDEQLESLFQRTAWVFDDKYKRPGYGAYDAF
KHAVSDPSILDSLDLNEDEREVLINNINRRLT

Ligand information

>7syr Chain 2 (length=1697)

uaccugguugauccugccaguagcauaugcuugucucaaagauuaagcca
ugcaugucuaaguacgcacggccgguacagugaaacugcgaauggcucau
uaaaucaguuaugguuccuuuggucgcucgacuuggauaacugugguaau
ucuagagcuaauacaugccgacgggcgcugacccccuucgcgggggggau
gcgugcauuuaucaguggugacucuagauaaccucgggccgaucgcacgc
ccggcggcgacgacccauucgaacgucugcccuaucaacuuucgauggua
gucgccgugccuaccauggugaccacgggugacggggaaucaggguucga
uuccggagagggagccugagaaacggcuaccacauccaaggaaggcagca
ggcgcgcaaauuacccacucccgacccggggagguagugacgaaaaauaa
caauacaggacucuuucgaggcccuguaauuggaaugaguccacuuuaaa
uccuuuaacgaggauccauuggagggcaagucuggugccagcagccgcgg
uaauuccagcuccaauagcguauauuaaaguugcugcaguuaaaaagcuc
guaguuggaucuugggagcggccguccccugcucggcgccggcccgaagc
guuuacuuugaaaaaauuagaguguucaaagcaggccgccuggauaccgc
agcuaggaauaauggaauaggaccgcgguucuauuuuguugguuuucgga
acugaggccaugauuaagagggacggccgggggcauucguauugcgccgc
uagaggugaaauucuuggaccggcgcaagacggaccagagcgaaagcauu
ugccaagaauguuuucauuaaucaagaacgaaagucggagguucgaagac
gaucagauaccgucguaguuccgaccauaaacgaugccgaccggcgaugc
ggcggcguuauucccaugacccgccgggcagcuuccgggaaaccaaaguc
uuuggguuccggggggaguaugguugcaaagcugaaacuuaaaggaauuu
ggcgaagggcaccaccaggaguggagccugcggcuuaauuugacucaaca
cgggaaaccucacccggcccggacacggacaggauugacagauugauagc
ucuuucucgauuccgugggugguggugcauggccguucuuaguuggugga
gcgauuugucugguuaauuccgauaacgaacgagacucuggcaugcuaac
uaguuacgcgaccccggucggcguaacuucuuagagggacaaguggcguu
cagccacccgagauugagcaauaacaggucugugaugcccuuagaugucc
ggggcugcacgcgcgcuacacugacuggcucagcgugugccuacccuacg
ccggcaggcgcggguaacccguugaaccccauucgugauggggaucgggg
auugcaauuauuccccaugaacgaggaauucccaguaagugcgggucaua
agcuugcguugauuaagucccugcccuuuguacacaccgcccgucgcuac
uaccgauuggaugguuuagugaggcccucggaucggccccgccggggugc
ccuggcggagcgcugagaagacggucgaacuugacuaucuagaggaagua
aaagucguaacaagguuuccguaggugaaccugcggaaggaucauua

...<<<<<.[.((((>>>>><<<.<<<<<<...<.<<.........<<<.
<<<..<<....<<....<<..........>>...>>.>>......<<...
.....<<<.<....<<....<<<<.........<<.....<<..<<....
.>>..>>......>>.........<<<<...<<<<<......>>>>>...
>>>><<..<<<<<...<......>..>>>>>......>>...<<<<.<<<
...>>>>>>>..>.>>>...>>..>.>>>.<<<....<<<....<<<<<<
<.........>>>>>>>>>>......>>>...<<<.<<<<....>>>>..
..>>>.>>.<<.<<<..........>>>.>>...<<<..>>>.....>>>
>>>.........<<<....<<<.....>>>..>>>......>>.>.....
<<<<<<<<<.............>>>>>>.>>>......<<..<.......
....>..>>.........<<<<<((......<<<<.....<<..))>>..
.....>>>>.>>>>>..>>>>>>.>>>.........<.<((.....<.<<
...<<<.<<....<<<.<<<..>>>.>>><<<<<<<.<<..>>>>>>.>>
>.<<<<<<.<.......<<...<.......>.<<<<.>>>>...>>....
..>.>>>..>>>........<.<<.<<<<<<.................>>
>>>>.>>.>....>>....<<<<<<......<<<<..<<..<<<<<<<<.
..<<<......>>>......>>>>>>>>..>>.......<<....>>...
>>>>.....>>>.>>>...>>>...>>.>....<<<<<<<...<...<<<
<.<.....>.>>>>...>>>>>>>>..........<<<.<<.<<<..<.<
<<<<<.<<<<......>>>>>>>>>>.>..>>>...<<..))>>...>>.
....>>>.>.>.<<<......<<<......>>>....>>>..)))).]..
<...<<<<<<<..<<..<<<<<..<<<.<<<<<<......<<........
>>..........<<<<<......<<<<<<.......<<.<<<........
>>>.>>.....>>>>>>...<<.<<<..<<.<<<<<.....<<<.<<<<<
....>>>...<<<......>>>...>>.>>>....<<<...<...<<<<.
.<<<<<<<<<<<<..>>>>.>>>>>>>>..>>>>..>.....<<<<<...
..>>>>>........>>>.....>>.>>>.....>>>>>>>.......>>
>>>...>>.>>>>.>>>.....<<.<<<........<.......<<<.<<
<<....>>>>.>>>....>.........>>>.>>......<.....<<<<
<..........>>>>>.....>.....>>>>>.....<<<<<<<<.....
..>>>>>>>>......>>...>>>>>>>..>.......<..<<.<..<<<
<.<<....<<<<<<<<.<<<..<<<<.<<...<<<<<..<<..<.<....
>.>..>>....>>>>>...>>.>>>>..>>>.>>>>>>>>...>>.>>>>
...>.>>...>.....<<<<<<<<<....>>>>>>>>>.........

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7syr Molecular architecture of 40S translation initiation complexes on the hepatitis C virus IRES.
• Resolution: 3.6 Å
• Binding residue (original residue number in PDB): R53 R54
• Binding residue (residue number reindexed from 1): R50 R51

Gene Ontology

Molecular Function

• GO:0003676 nucleic acid binding
• GO:0003723 RNA binding
• GO:0003743 translation initiation factor activity
• GO:0005515 protein binding
• GO:0043022 ribosome binding

Biological Process

• GO:0000423 mitophagy
• GO:0006412 translation
• GO:0006413 translational initiation
• GO:0006417 regulation of translation
• GO:0032057 negative regulation of translational initiation in response to stress
• GO:0034063 stress granule assembly
• GO:0034198 cellular response to amino acid starvation
• GO:0034599 cellular response to oxidative stress
• GO:0034605 cellular response to heat
• GO:0034644 cellular response to UV
• GO:0034976 response to endoplasmic reticulum stress
• GO:0036490 regulation of translation in response to endoplasmic reticulum stress
• GO:0036499 PERK-mediated unfolded protein response
• GO:0043558 regulation of translational initiation in response to stress
• GO:0140468 HRI-mediated signaling
• GO:1904373 response to kainic acid
• GO:1990737 response to manganese-induced endoplasmic reticulum stress
• GO:2000676 positive regulation of type B pancreatic cell apoptotic process

Cellular Component

• GO:0005634 nucleus
• GO:0005737 cytoplasm
• GO:0005739 mitochondrion
• GO:0005829 cytosol
• GO:0005850 eukaryotic translation initiation factor 2 complex
• GO:0010494 cytoplasmic stress granule
• GO:0016020 membrane
• GO:0033290 eukaryotic 48S preinitiation complex
• GO:0044207 translation initiation ternary complex
• GO:0045202 synapse
• GO:0070062 extracellular exosome
• GO:0097451 glial limiting end-foot

External links

• PDB: RCSB:7syr, PDBe:7syr, PDBj:7syr
• PDBsum: 7syr
• PubMed: 35822879
• UniProt: P05198|IF2A_HUMAN Eukaryotic translation initiation factor 2 subunit 1 (Gene Name=EIF2S1)