Structure of PDB 7ohv Chain j Binding Site BS01

Receptor Information

>7ohv Chain j (length=73) Species: 559292 (Saccharomyces cerevisiae S288C)

KSHTLCNRCGRRSFHVQKKTCSSCGYPAAKTRSYNWGAKAKRRHTTGTGR
MRYLKHVSRRFKNGFQTGSASKA

Ligand information

>7ohv Chain 1 (length=2141)

guuugaccucaaaucagguaggaguacccgcugaacuuaagcauaucaau
aagcggaggaaaagaaaccaaccgggauugccuuaguaacggcgagugaa
gcggcaaaagcucaaauuugaaaucugguaccuucggugcccgaguugua
auuuggagagggcaacuuuggggccguuccuugucuauguuccuuggaac
aggacgucauagagggugagaaucccguguggcgaggagugcgguucuuu
guaaagugccuucgaagagucgaguuguuugggaaugcagcucuaagugg
gugguuccaucuaaagcuaaauauuggcgagagaccgauagcgaacaagu
acagugauggaaagaugaaaagaacuuugaaaagagagugaaaaaguacg
ugaaauuguugaaagggaagggcauuugaucagacaggccagcaucaguu
uugguggcaggauaaauccauaggaauguagcuugccucgguaaguauua
uagccugugggaauacugccagcugggacugaggacugcgacguaaguca
aggaugcuggcauaaugguuauaugccgcccgucuugaaacacggaccaa
ggagucuaacgucuaugcgaguguuuggguguaaaacccauacgcguaau
gaaagugaacguagguuggggccucgcaagaggugcacaaucgaccgauc
cugaggauggauuugaguaagagcauagcuguugggacccgaaagauggu
gaaagccagcugguggaccaucuaguagcugguuccugccgaaguuuccc
uaggauagcagaagcucguaucaguuuuauguaaaaugaagagcuuuuag
ugggccauuuuugguaagcagaacuggcgaugcgggaugaaccgaacgua
gaguuaaggugccggaauacacgcucaucagacaaaagguguuaguucau
cuagacagccggacgguggccauggaagucggaauccgcuaaggagugug
uaacaacucaccggccgaaugaacuagcccugaaggauggcgcucaagcg
uguuaccuauacucuaccgucaggguugauaugaugcccugacgaguagg
caggcguggaggucagugacgaagccuagaccguaaggucgggucgaacg
gccucuagugcagaucuuggugguaguagcauucaaaugagaacuuugaa
gacugaaggggaaagguuccacgucaacagcaguuggacguggguuaguc
gauccuaagagauggggaagcuccguggccugauuuuaugcaggccacca
ucgaaagggaauccgguuaagauuccggaaccuggauauggauucuucac
gguaacguaacugaauguggagacgucggcgcgagcccugggaggaguua
ucuuuucuucuuaacagcuuaucaccccggagguuuauccgggauggggu
cuuauggcuggaagaggccagcacugcuggcuccgguggcuugugacggc
cugaaaauccacaggaaggaauaguuuucaugccaggucguacugcucca
aggugacagccucuaguugauagaauaauguacuacuaucuagcgaaacc
acagccaagggaacgggcuuggcagaaucagcgggacucuaguuugacau
uauugucaggugggagugcuuguggcagucaagcgagcgacauugcuauc
auaccgaagcagaauucgguaagcgucuaauaccuacugaugaauguuac
cgcaauaguaauugaacuuaguacgagaggaacaguucauucggauaauu
gguuuuugcggcugucugaucaggcaugccgcgaagcuaccauccgcugg
auuauggcugaacgccucuaagucagaauccaugcuagaacgcggugauu
ucuuugcuccacacaauauagauggauacgaauaaggcguccuuguggcg
ucgcugaaccauagcaggcuagcaacggugcacuuggcggaaaggccuug
ggugcuugcuggcgaauugcaaugucauuuugcguggggauaaaucauuu
guauacgacuuagauguacaacgggguauuguaagcaguagaguagcugu
guuacgaucugcugagauuaagccuuuguugucugauuugu

...........................<<<<<.....<<....>>.....
.>>>>>...........<<....>>..<<<<<......<<.....>>...
..>>>>>..<<<..<........<<.<<<<<<...>>>>>>.>>......
..>....<<<<<<<.<<<<<<<<<<<<<<<<<<<<<....<<<<......
>>>>.((......<<<.....))>>>..>.>>>>>>>>..>>>>>>>>..
..>>>>>>>>>>>........<<<<<<<........>>>>>>>.....<<
<<<<..>>>>>>..>>>.................................
.<<....>>...............<<<<....>>>>..............
....................<<<<<.<<<<<......<<<<<<<.<<<<<
<<<<<<<<<<......<<<<<<<<......<<<<<<....>>>>>>....
...>>>>>>>.>...>>>>>.>>>>>>>>>>........<<<....>>>.
....>>>>>>>.....>>>>>.>>>>>..<<<<........>>>><<<<<
<....<<<<<<.<<<<<<..<<<...<<<<.....>>>>...>>>...<<
....>>.......<<<<<..<<<<<....>>>>>...>>>>>.<<<<<<<
<.......>>>.>>>.>>....>>>>>>.>>>>>>..<<.<..<<<<<<<
....<<<<..>>>>..>>>>>>>.....>.>>..<<<<<<....<.<...
....>.><<<<<<<<<....<<<.<<<<<..>>>>>>>>..>>>>>>>.>
>..<<<<.<<<<.......>>>>.>>>>....<<<......>>>...<<<
<<<<..<<<<(((.....<<<<<<.....<<.....<<<.<<<<<<<<<<
.......<<<<<.<<....<<.........>>....>>.........<<.
.....>>...>>>>>...>>>>>>>>>>>>>.......))).>>...>>>
>>>.>>>>..>>>>>>><<<<<<<<<.........>>>>>>>>>....>>
>>>><..<<<<<<<.<<......<<<<.<<<<....>>>>>>>>...>>>
>>>>>>...>.....>>>>>><<.....<<.<<<<<<.......>>>>>>
<<<<<..<<<...<<...<<<<<<...<<.....>>.>>>>>>....((<
<<<<<...<<....<<<....>>>..<<<<<<........>>>>>>....
>>....>>...<<<<<........>>>>>))<<<<.<<<<<<......<<
<....>>>...<...<<<<<<...<<<<.<<<<<<<<<<<<<<<<<<...
....>>>>>><...<<<<<....<<<<<<..<<.....>>....>>>>>>
.....>>>>>....><<<<<<<...>>>>>>>>>>>.>>>>>>>>.>>>>
.......>>>>>>...>........>>.>>>>>>>>>>>>..>>..>>>.
<<<<....>>>>.>>>>>..<<<<.....<<<.>>>..>>>>>>....>>
....<<<<<........>>>>>....<<<<<..<<<<<<<..<<<<<<<.
...>>>>>>>..>>>>>........<<<<...<<..>>...>>>>....<
.<<<<<<<......>>>>>>>...>........>>.>>>>>....<<<<<
<<<..<<<<.<.<<<<<<...............>>>>>>.><<<<<...<
<<<....<<<<<<<<<<....>>>>>.>>>>>...>>>>..>>>>>.<<<
<...<<<<<...........>>>>>...>>>>.>>>>....>>>>>>>>.
......<<<<<<.<<<.<<....<<..........<<<<<<.....>>>>
>>......>>.......<<<<<<...<<<<<.<..<<<......>>>..>
.>>>>>.>>>>>>.............>>.>>>.>>>>>>..<<<<<.<..
...........>.....<<<<<<<<<.<<<....<<<<....<<<<....
.>>>>...>>>>...>>>....>>>>.>>>>>..>>>>>..

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7ohv Analysis of subunit folding contribution of three yeast large ribosomal subunit proteins required for stabilisation and processing of intermediate nuclear rRNA precursors.
• Resolution: 3.9 Å
• Binding residue (original residue number in PDB): H16 R24 R25 H28 K31 S35 S36 A42 K43 T44 R45 Y47 N48 W49 K52 A53 R55 R56
• Binding residue (residue number reindexed from 1): H3 R11 R12 H15 K18 S22 S23 A29 K30 T31 R32 Y34 N35 W36 K39 A40 R42 R43

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003735 structural constituent of ribosome
• GO:0008270 zinc ion binding
• GO:0019843 rRNA binding
• GO:0046872 metal ion binding

Biological Process

• GO:0000448 cleavage in ITS2 between 5.8S rRNA and LSU-rRNA of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
• GO:0002181 cytoplasmic translation
• GO:0006412 translation

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:0030687 preribosome, large subunit precursor
• GO:0044391 ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:7ohv, PDBe:7ohv, PDBj:7ohv
• PDBsum: 7ohv
• PubMed: 34813592
• UniProt: P49166|RL37A_YEAST Large ribosomal subunit protein eL37A (Gene Name=RPL37A)