Structure of PDB 7oh3 Chain j Binding Site BS01

Receptor Information

>7oh3 Chain j (length=87) Species: 559292 (Saccharomyces cerevisiae S288C)

GKGTPSFGKRHNKSHTLCNRCGRRSFHVQKKTCSSCGYPAAKTRSYNWGA
KAKRRHTTGTGRMRYLKHVSRRFKNGFQTGSASKASA

Ligand information

>7oh3 Chain 1 (length=3013)

guugaccucaaaucagguaggaguacccgcugaacuuaagcauaucaaua
agcggaggaaaagaaaccaaccgggauugccuuaguaacggcgagugaag
cggcaaaagcucaaauuugaaaucugguaccuucggugcccgaguuguaa
uuuggagagggcaacuuuggggccguuccuugucuauguuccuuggaaca
ggacgucauagagggugagaaucccguguggcgaggagugcgguucuuug
aaagugccuucgaagagucgaguuguuugggaaugcagcucuaagugggu
gguaaauuccaucuaaagcuaaauauuggcgagagaccgauagcgaacaa
guacagugauggaaagaugaaaagaacuuugaaaagagagugaaaaagua
cgugaaauuguugaaagggaagggcauuugaucagacaggccagcaucag
uuuugguggcaggauaaauccauaggaauguagcuugccugguaaguauu
auagccugugggaauacugccagcugggacugaggacugcgacguaaguc
aaggaugcuggcauaaugguuauaugccgcccgucuugaaacacggacca
aggagucuaacgucuaugcgaguguuuggguguaaaacccauacgcguaa
ugaaagugaacguagguuggggccucgcaagaggugcacaaucgaccgau
ccugaugucuucggauggauuugaguaagagcauagcuguugggacccga
aagauggugaacuaugccugaauagggugaagccagaggaaacucuggug
gaggcucguagcgguucugacgugcaaaucgaucgucgaauuuggguaua
ggggcgaaagacuaaucgaaccaucuaguagcugguuccugccgaaguuu
cccucaggauagcagaagcucguaucaguuuuaugagguaaagcgaaaac
uuuaaauauguaagaaguccuuguuugaacguggacaugaaugaagagcu
uuuagugggccauuuuugguaagcagaacuggcgaugcgggaugaaccga
acguagaguuaaggugccggaauacacgcucaucagacacacaaaaggug
uuaguucaucuagacagccggacgguggccauggaagucggaauccgcua
aggaguguguaacaacucaccggccgaaugaacuagcccugaaaauggau
ggcgcucaagcguguuaccuauacucuaccgucaggguugugaugcccug
acgaguaggcaggcguggaggucagugacgaagccuagaccguaaggucg
ggucgaacggccucuagugcagaucuuggugguaguagcaaauauucaaa
ugagaacuuugaagacugaaguggggaaagguuccacgucaacagcaguu
ggacguggguuagucgauccuaagagauggggaagcuccguuucaaaggc
cuaggccaccaucgaaagggaauccgguuaagauuccggaaccuggauau
ggauucuucacgguaacguaacugaauguggagacgucggcgcgagcccu
gggaggaguuaucuuuucuucuuaacagcuuaucaccccggaauugguuu
auccggagauggggucuuauggcuggaagaggccagcacugcuggcuccg
gugcgcuugugacggcccgugaaaauccacaggaaggaauaguuuucaug
ccaggucguacugauaaccgcagcaggucuccaaggugaacagccucuag
uugauagaauaauguagauaagggaagucggcaaaauagauccguaacuu
cgggauaaggauuggcucuaagggucggacgaucaacuuagaacugguac
ggacaaggggaaucugacugucuaauuaaaacauagcauugcgaugguca
gaaagugauguugacgcaaugugauuucgcccagugcucugaaugucaaa
gugaagaaauucaaccaagcgcggguaaacggcgggaguaugacucucuu
aagguagccaaauggauuaacgagauucccacugucccuaucuacuaucu
agcgaaaccacagccaagggggcuuggcagaaucagcggggaagaagacc
cugugagcuugacucuaguuugacauugugaagagacauaguuucuuuac
uuauucaaugaagcggagcuggaauuauuuuccacguucuagcauucaag
gucccucggggcugauccgguugaagacauugucagguggggaguuuggg
ggcggcacaucuguuaaacgauaagcagauguccuaaggggggcucaugg
agaacagaaaucuccaguagaacaaaaggguaaaagcccccuugauuuug
auuuucagugugaauacaaaccaugaaaguguggccuaucgaucuuuagu
cccucggaauuugaggcuagaggugccagaaaguuaccacagggauaacu
ggcuuguggcagucaagcguucagcgacauugcuuuugauucuucgaugu
cggcucuuccuaucauaccgaagcagaauucgguaagcguuggauuguuc
acccacuaauagggaguagcuggguuuagaccgucgugagacagguuagu
uuacccuacugaugaauguuaccgcaauaguaauugaacuuaguacgaga
ggaacaguucauucggauaauugguuuuugcggcugucugaucaggcauu
gccgcgaagcuaccauccgcuggauuauggcugaacgccucuaagucaga
auccaugcuagaacgcggugauuucugcuccacacaauauagauggauac
gaauaaggcguccuuguggcgucgcugaaccauagcaggcuagcaacggu
gcacuuggcggaaaggccuugggugcuugcuggcgaauugcaaugucauu
uugcguggggauaaaucauuuguauacgacuuagauguacaacgggguau
uguaagcaguagaguagccuugguuacgaucugcugagauuaagccuuug
uugucugauuugu

.........................<<<<<<.....<<....>>......
>>>>>.>.........<<....>>..<<<<<......<<.....>>....
.>>>>>..<<<..<<.......<<.<<<<<<...>>>>>>.>>.......
>>....<<<<<<.<<<<<<<<<<<<<<<<<<<<<<....<<<<......>
>>>.((......<<<.....))>>>..>.>>>>>>>>..>>>>>>>>...
>>>>>>>>>>>........<<<<<<<........>>>>>>>.....<<<<
<..((((((>>>>>..>>>...............................
...<<....>>...............<<<<....>>>>............
......................<<<<<.<<<<<......<<<<<<<.<<<
<<<<<<<<<<<<.....<<<<<<<<<......<<<<<<...>>>>>>...
....>>>>>>>.>.>.>>>>>.>>>>>>>>>>........<<<....>>>
.....>>>>>>>.....>>>>>.>>>>>..<<<<........>>>><<<<
<<....<<<<<<.<<<<<<..<<<...<<<<.....>>>>...>>>...<
<....>>.......<<<<<..<<<<<....>>>>>...>>>>>.<<<<<<
<<...<......>...>>>.>>>.>>....>>>>>>.>>>>>>..<<.<.
..<<<<<<.....<<<<<<<<<..<<<....<<<<<<<....>>>>>>>.
....>>>...<<<<.<<.<<........>>>>>>>>....>>>>>>>>>.
..<<.......>>......>>>>>>......>.>>..<<<<<<....<.<
<<....>>>.><<.<<<<......<<<.<<<<<<....<..<........
.>..>....>>>>...<<<<.<<<....>>>.>>>>...>>>>>....>>
>>..>>..<<<<.<<<<<.....>>>>>.>>>>....<<<......>>>.
..<<<<<<<..<<<<(((.....<<<<<<.....<<.........<<<.<
<<<<<<<<<.......<<<<<.<<....<<.........>>....>>...
......<<......>>...>>>>>...>>>>>>>>>>>>>..........
))).>>...>>>>>>.>>>>..>>>>>>><<<<<<<<<......>>>>>>
>>>....>>>>>><.<<<<<<<<.<<......<<<<.<<<<....>>>>>
>>>...>>>>>>>>>>..>.....>>>>>><<.....<<.....<<<<<<
.......>>>>>><<<<.....<<<<<.<<...<<<<<<...<<.....>
>.>>>>>>....((<<<<<<...<<....<<<....>>>........<<<
<..>>>>....>>....>>...<<<<<........>>>>>))<<<<.<<<
<<<<.....<<<....>>>...<.{{<<<<<<...<<<<.<<<<<<<.<<
<<<<<<<<..[[[..>>>>>><...<<<<<....<<<<<<....<<<...
..>>>....>>>>>>.....>>>>>....><<<<<<<...>>>>>>>>>>
>...>>>>>>>.>>>>.........>>>>>>...>......>>>..>>>>
>>>>>>>>..>>.....<<......>>>>>>>.<<<<.....>>>>..>>
>>..<<<<.....<<<<<<<.<<.........<.....<<<<<.......
........>>>>><<.<<<<<<<<<<<..>>>>>..>>>>>>.>>.....
.......<<<<<<<<<..<<<.........>>>..<<<<<<<....<<<<
.....>>>>......>>>>>>>.............<<<.<<..<<<....
.<<<<....>>>>.....>>>>>>>>........<<.<......>.>>..
........<<..>>......>>.>>>>>>>..>..>>.>>>>>>>..>>>
>>>....>>....<<<<<....>>>>>....<<<<<..<<<<......<<
<<<<<<<<<..<<<<<.<<<<<<<<..<<<<<<<<<<...>>>>>>>>>>
...<<<<<.....<<<<...<<<<.....>>>>...........}}...<
<<<<<..>>>>>>..>>>>.>>>>>.....>>>>>>>>..>>>>>.....
.<..<...<<<<<<..........>>>>>>.......<<<<<<<...<<<
<<<.......>>>>>>.....<<......>>....>>>>>>>...<<<..
<<<<.......>>>>..>>>.<<<....>>>...>.>......<<<<<..
.<<<<.))))))>>>>.>>>>>.........>>>>..>>>>>>>......
.......<<<<<<...<<.....>>...>>>>>>................
<<<<...<<<<..<.<<<<<<<......>>>>>>>...>.<<<.......
..>>>.....>>>>....>>>>......<<<<<<<....>>>.>>>>...
.>..>>>.>>>>>....<<<<<<<<..<<<<.<.<<<<<<..........
.....>>>>>>.><<<<<...<<<<...<<<<<<<<<<<]]].>>>>>..
>>>>>>..>>>>..>>>>>.<<<<...<<<<<...........>>>>>..
.>>>>.>>>>....>>>>>>>>.....<<<<<<.<<<.<<....<<....
......<<<<<<.....>>>>>>......>>.......<<<<<<...<<<
<<.<..<<<......>>>..>.>>>>>.>>>>>>.............>>.
>>>.>>>>>>..<<<<<.<..<.......>..>.....<<<<<<<<<.<<
<....<<<<....<<<<<....>>>>>...>>>>...>>>....>>>>.>
>>>>..>>>>>..

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7oh3 Analysis of subunit folding contribution of three yeast large ribosomal subunit proteins required for stabilisation and processing of intermediate nuclear rRNA precursors.
• Resolution: 3.4 Å
• Binding residue (original residue number in PDB): K3 G4 T5 P6 F8 G9 K10 R11 H12 N13 K14 S15 H16 R24 R25 H28 Q30 K31 S35 S36 K43 T44 R45 Y47 N48 W49 K52 A53 R55 R56 K75
• Binding residue (residue number reindexed from 1): K2 G3 T4 P5 F7 G8 K9 R10 H11 N12 K13 S14 H15 R23 R24 H27 Q29 K30 S34 S35 K42 T43 R44 Y46 N47 W48 K51 A52 R54 R55 K74

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003735 structural constituent of ribosome
• GO:0008270 zinc ion binding
• GO:0019843 rRNA binding
• GO:0046872 metal ion binding

Biological Process

• GO:0000448 cleavage in ITS2 between 5.8S rRNA and LSU-rRNA of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
• GO:0002181 cytoplasmic translation
• GO:0006412 translation

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:0030687 preribosome, large subunit precursor
• GO:0044391 ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:7oh3, PDBe:7oh3, PDBj:7oh3
• PDBsum: 7oh3
• PubMed: 34813592
• UniProt: P49166|RL37A_YEAST Large ribosomal subunit protein eL37A (Gene Name=RPL37A)