Structure of PDB 6yly Chain j Binding Site BS01

Receptor Information

>6yly Chain j (length=81) Species: 4932 (Saccharomyces cerevisiae)

PSFGKRHNKSHTLCNRCGRRSFHVQKKTCSSCGYPAAKTRSYNWGAKAKR
RHTTGTGRMRYLKHVSRRFKNGFQTGSASKA

Ligand information

>6yly Chain 1 (length=2676)

uugaccucaaaucagguaggaguacccgcugaacuuaagcauaucaauaa
gcggaggaaaagaaaccaaccgggauugccuuaguaacggcgagugaagc
ggcaaaagcucaaauuugaaaucugguaccuucggugcccgaguuguaau
uuggagagggcaacuuuggggccguuccuugucuauguuccuuggaacag
gacgucauagagggugagaaucccguguggcgaggagugcgguucuuugu
aaagugccuucgaagagucgaguuguuugggaaugcagcucuaagugggu
gguaaauuccaucuaaagcuaaauauuggcgagagaccgauagcgaacaa
guacagugauggaaagaugaaaagaacuuugaaaagagagugaaaaagua
cgugaaauuguugaaagggaagggcauuugaucagacaggccagcaucag
uuuugguggcaggauaaauccauaggaauguagcuugccucgguaaguau
uauagccugugggaauacugccagcugggacugaggacugcgacguaagu
caaggaugcuggcauaaugguuauaugccgcccgucucacggaccaagga
gucuaacgucuaugcgaguguuuggguguaaaacccauacgcguaaugaa
agugaacguagguuggggccucgcaagaggugcacaaucgaccgauccug
augucuucggauggauuugaguaagagcauagcuguugggacccgaaaga
uggugaacuaugccugaauagggugaagccagaggaaacucugguggagg
cucguagcgguucugacgugcaaaucgaucgucgaauuuggguauagggg
cgaaagacuaaucgaaccaucuaguagcugguuccugccgaaguuucccu
caggauagcagaagcucguaucaguuuuaugagaauauguaagaaguccg
acauuugaaugaagagcuuuuagugggccauuuuugguaagcagaacugg
cgaugcgggaugaaccgaacguagaguuaaggugccggaauacacgcuca
ucagacaccacaaaagguguuaguucaucuagacagccggacgguggcca
uggaagucggaauccgcuaaggaguguguaacaacucaccggccgaauga
acuagcccugaaaauggauggcgcucaagcguguuaccuauacucuaccg
ucaggguugauaugaugcccugacgaguaggcaggcguggaggucaguga
cgaagccuagaccguaaggucgggucgaacggccucuagugcagaucuug
gugguaguagcaaauauucaaaugagaacuuugaagacugaaguggggaa
agguuccacgucaacagcaguuggacguggguuagucgauccuaagagau
ggggaagcuccguuucaaaggccugacaggccaccaucgaaagggaaucc
gguuaagauuccggaaccuggauauggauucuucacgguaacguaacuga
auguggagacgucggcgcgagcccugggaggaguuaucuuuucuucuuaa
cagcuuaucaccccggaauugguuuauccggagauggggucuuauggcug
gaagaggccagcaccuuugcuggcuccggugcgcuugugacggcccguga
aaauccacaggaaggaauaguuuucaugccaggucguacugauaaccgca
gcaggucuccaaggugaacagccucuaguugauagaauaauguagauaag
ggaagucggcaaaauagauccguaacuucgggauaaggauuggcucuaag
ggucggacgaucaacuuagaacugguacggacaaggggaaucugacuguc
uaauuaaaacauagcauugcgauggucagaaagugauguugacgcaaugu
gauuuauggauuaacgagauucccacugucccuaucuacuaucuagcgaa
accacagccaaggggcuuggcagaaucagcggggacuaguuugacauugu
gaagagacauaguuucuuuacuuauucaaugaagcggagcuggaauucau
uuuccacguucuagcauucaaggucccauucggggcugauccggguugaa
gacauugucaggugguagucccucggaauuugaggcuaaacuggcuugug
gcagucaagcguucauagcgacauugcuuuuugauucuucgaugucggcu
cuuccuaucauaccgaagcagaauucgguaagcguuggauuguucaccca
cuaauagggauacccuacugaugaauguuaccgcaauaguaauugaacuu
aguacgagaggaacaguucauucggauaauugguuuuugcggcugucuga
ucaggcauugccgcgaagcuaccauccgcuggauuauggcugaacgccuc
uaagucagaauccaugcuagaacgcggugauuucuuugcuccacacaaua
uagauggauacgaauaaggcguccuuguggcgucgcugaaccauagcagg
cuagcaacggugcacuuggcggaaaggccuugggugcuugcuggcgaauu
gcaaugucauuuugcguggggauaaaucauuuguauacgacuuagaugua
caacgggguauuguaagcaguagaguagccuuguuguuacgaucugcuga
gauuaagccuuuguugucugauuugu

........................<<<<<<.....<......>......>
>>>>.>.........<<....>>..<<<<<......<<.....>>.....
>>>>>..[[[...........<<.<<<<<<...>>>>>>.>>........
.....<<<<<<<.<<<<<<<<<<<<<<<<<<<<.....<<<<......>>
>>.(.......<<<......)>>>....>>>>>>>>..>>>>>>>>....
>>>>>>>>>>>........<<<<<<<........>>>>>>>.....((((
(..<<<<<<)))))..]]]...............................
...<<....>>...............<<<<....>>>>............
......................<<<<<.<<<<<......<<<<<<<.<<<
<<<<<<<<<<<<.....<<<<<<<<<......<<<<<<....>>>>>>..
.....>>>>>>>.>.>.>>>>>.>>>>>>>>>>........<<<....>>
>.....>>>>>>>.....>>>>>.>>>>>..<<<<...>>>><<<<<<..
..<<<<<<.<<<<<<..<<<...<<<<.....>>>>...>>>...<<...
.>>.......<<<<<..<<<<<....>>>>>...>>>>>.<<<<<<<<..
.<<....>>...>>>.>>>.>>....>>>>>>.>>>>>>..<<.<...<<
<<<<.....<<<<<<<<<..<<<....<<<<<<<....>>>>>>>.....
>>>...<<<<.<<.<<........>>>>>>>>....>>>>>>>>>...<<
.......>>......>>>>>>......>.>>..<<<<<<......<<<..
..>>><.<<<<<<<<<<...<<<.<<<<<<<......>>>>>...<<<.>
>>.....>>>>>.>>>>>>>>.>>>.<<<<.<<<<<.....>>>>>.>>>
>....<<<......>>>...<<<<<<<..<<<<(((.....<<<<<<...
...<..........<<<.<<<<<<<<<<.......<<<<<.<<....<<.
........>>....>>.........<<......>>...>>>>>...>>>>
>>>>>>>>>..........))).>....>>>>>>.>>>>..>>>>>>><<
<<<<<<<.........>>>>>>>>>....>>>>>><<.<<<<<<<.<<..
....<<<<.<<<<....>>>>>>>>...>>>>>>>>>..>>.....>>>>
>><<.....<<.....<<<<<<.......>>>>>><<<<.....<<<<<.
<<...<<<<<<...<<.....>>.>>>>>>....((<<<<<<...<<...
.<<<....>>>........<<<.<....>>>>....>>....>>>..<<<
<<........>>>>>))<<<<.<<<<<<<.....<<<....>>>...<.{
{<<<<<<...<<<<.<<<<<<<<<<<<<<<<<<..[[[..>>>>>>....
<<<<<....<<<<<<....<<<.....>>>....>>>>>>.....>>>>>
.....<<<<<<<<....>>>>>>>>>>>>.>.>>>>>>>.>>>>......
...>>>>>>...>......>>>..>>>>>>>>.>>>..>>.....<<...
...>>>>>>>.<<<<.....>>>>..>>>>..<<<<.....<<<<<<<.<
<...............<<<<<....<....>.....>>>>><<.<<<<<<
<<<<<..>>>>>..>>>>>>.>>............<<<<<<<<<..<<<.
........>>>..<<<<<<<....<<<<.....>>>>......>>>>>>>
.<.<.....>.>..>>.>>>>>>>.....>>.>>>>>>>..>>>>>>...
.>>....<<<<<...>>>>>....(((((..[[[....<<<<<<<<..<<
<<<<<<<<...>>>>>>>>>>...<<<<<.....<<<<..<<<<<.....
.>>>>>..........}}...<<<<<<....>>>>>>..>>>>..>>>>>
.....>>>>>>>>..[[[..((((.>>>>>>))))]]]............
<<<<<...<<.......>>...>>>>>................(......
.<<<<<..<.<<<<<<<......>>>>>>>...>.<<<....)....>>>
.....>>>>>...]]].)))))....<<<<<<<<..<<<<.<.<<<<<<.
..............>>>>>>.><<<<<....<<<....<<<<<<<<<.]]
]..>>>>..>>>>>...>>>...>>>>>.<<<<...<<<<<.........
..>>>>>...>>>>.>>>>....>>>>>>>>.......<<<<<<.<<<.<
.....<<..........<<<<<<.....>>>>>>......>>.......<
<<<<<...<<<<<.<..<<<......>>>..>.>>>>>.>>>>>>.....
.........>.>>>.>>>>>>..<<<<<.<.............>.....<
<<<<<<<<.<<<....<<<<....<<<<.<.....>>>>>...>>>>...
>>>....>>>>.>>>>>..>>>>>..

Receptor-Ligand Complex Structure

Structure summary

• PDB: 6yly Construction of the Central Protuberance and L1 Stalk during 60S Subunit Biogenesis.
• Resolution: 3.8 Å
• Binding residue (original residue number in PDB): P6 S7 F8 G9 K10 R11 H12 N13 S15 H16 R24 R25 S26 H28 Q30 S35 K43 R45 Y47 N48 W49 K52 R55 R56 K75
• Binding residue (residue number reindexed from 1): P1 S2 F3 G4 K5 R6 H7 N8 S10 H11 R19 R20 S21 H23 Q25 S30 K38 R40 Y42 N43 W44 K47 R50 R51 K70

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003735 structural constituent of ribosome
• GO:0008270 zinc ion binding
• GO:0019843 rRNA binding
• GO:0046872 metal ion binding

Biological Process

• GO:0000448 cleavage in ITS2 between 5.8S rRNA and LSU-rRNA of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
• GO:0002181 cytoplasmic translation
• GO:0006412 translation

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:0030687 preribosome, large subunit precursor
• GO:0044391 ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:6yly, PDBe:6yly, PDBj:6yly
• PDBsum: 6yly
• PubMed: 32668200
• UniProt: P49166|RL37A_YEAST Large ribosomal subunit protein eL37A (Gene Name=RPL37A)