Structure of PDB 7ls2 Chain i2 Binding Site BS01

Receptor Information

>7ls2 Chain i2 (length=103) Species: 10090 (Mus musculus)

VNVPKTRRTFCKKCGKHQPHKVTQYKKGKDSLYAQGKRRYDRKQSGYGGQ
TKPIFRKKAKTTKKIVLRLECVEPNCRSKRMLAIKRCKHFELGGDKKRKG
QVI

Ligand information

>7ls2 Chain A2 (length=3706)

cgcgaccucagaucagacguggcgacccgcugaauuuaagcauauuaguc
agcggaggaaaagaaacuaaccaggauucccucaguaacggcgagugaac
agggaagagcccagcgccgaauccccgccgcgcgucgcggcgugggaaau
guggcguacggaagacccacuccccggcgccgcucguggggggcccaagu
ccuucugaucgaggcccagcccguggacggugugaggccgguagcggccc
ccggcgcgccgggcucgggucuucccggagucggguugcuugggaaugca
gcccaaagcgggugguaaacuccaucuaaggcuaaauaccggcacgagac
cgauagucaacaaguaccguaagggaaaguugaaaagaacuuugaagaga
gaguucaagagggcgugaaaccguuaagagguaaacgggugggguccgcg
caguccgcccggaggauucaacccggcggcguccggccgugccggugguc
cggcggaucuuucccgcgcgggggaccgcccccggccggcgaccggccgc
cgccgggcgcacuuccaccguggcggugcgccgcgaccggcuccgggacg
gccgggaaggcccgguggggaagguggcucggggggggcucaccccgagu
guuacagcccuccggccgcgcuuuucgccgaaucccggggccgaggaaga
ccgucgccgcgcucucccucucccgggugggggccgggccgccccuccca
cggcgcgaccgcuccggggcggacuguccccagugcgccccgggcgucgu
cgcgccgucgggucccgggggcgucggucacgcgugacgaagccgagcgc
acggggucggcggcgaugucggcuacccacccgacccgucuugaaacacg
gaccaaggagucuaacgcgugcgcgagucaggggcucguccgaaagccgc
cguggcgcaaugaaggugaagggccccgcccgggggcccgaggugggauc
ccgaggccucuccaguccgccgagggcgcaccaccggcccgucucgcccg
ccgcgccggggagguggagcacgagcguacgcguuaggacccgaaagaug
gugaacuaugcuugggcagggcgaagccagaggaaacucugguggagguc
cguagcgguccugacgugcaaaucggucguccgaccuggguauaggggcg
aaagacuaaucgaaccaucuaguagcugguucccuccgaaguuucccuca
ggauagcuggcgcucucgcuacgcaguuuuauccgguaaagcgaaugauu
agaggucuuggggccgaaacgaucucaaccuauucucaaacuuuaaaugg
guaagaagcccggcucgcuggcguggagccgggcguggaaugcgagugcc
uagugggccacuuuugguaagcagaacuggcgcugcgggaugaaccgaac
gccggguuaaggcgcccgaugccgacgcucaucagaccccagaaaaggug
uugguugauauagacagcaggacgguggccauggaagucggaauccgcua
aggaguguguaacaacucaccugccgaaucaacuagcccugaaaauggau
ggcgcuggagcgucgggcccauacccggccgucgccggcagucggaacgg
aacgggacgggagcggcgucgggccccgcggagccuacgccgcgacgagu
aggagggccgcugcggugagccuugaagccuagggcgcgggcccgggugg
agccgccgcaggugcagaucuuggugguaguagcaaauauucaaacgaga
acuuugaaggccgaaguggagaaggguuccaugugaacagcaguugaaca
ugggucagucgguccugagagaugggcgagugccguuccgaagggacggg
cgauggccuccguugcccucggccgaucgaaagggagucggguucagauc
cccgaauccggaguggcggagaugggcgccgcgaggcguccagugcggua
acgcgaccgaucccggagaagccggcgggagccucggggagaguucucuu
uucuuugugaagggcagggcgcccuggaauggguucgccccgagagaggg
gcccgugccuuggaaagcgucgcgguuccggcggcguccggugagcucuc
gcuggcccuugaaaauccgggggagaggguguaaaucucgcgccgggccg
uacccauauccgcagcaggucuccaaggugaacagccucuggcauguugg
aacaauguagguaagggaagucggcaagccggauccguaacuucgggaua
aggauuggcucuaagggcugggucggucgcggccggcgccuagcagccga
cuuagaacuggugcggaccaggggaauccgacuguuuaauuaaaacaaag
caucgcgaaggcccgcggcggguguugacgcgaugugauuucugcccagu
gcucugaaugucaaagugaagaaauucaaugaagcgcggguaaacggcgg
gaguaacuaugacucucuuaagguagccaaaugccucgucaucuaauuag
ugacgcgcaugaauggaugaacgagauucccacugucccuaccuacuauc
cagcgaaaccacagccaagggaacgggcuuggcggaaucagcggggaaag
aagacccuguugagcuugacucuagucuggcacggugaagagacaugaga
gguguagaauaagugggaggcccggccgccggugaaauaccacuacucuc
aucguuuuuucacugacccggugaggcgggggggcgagccccgaggggcu
cucgcuucuggcgccaagcguccgucccgcgcgugcgggcgggcgcgacc
cgcuccggggacagugccagguggggaguuugacuggggcgguacaccug
ucaaacgguaacgcagguguccuaaggcgagcucagggaggacagaaacc
ucccguggagcagaagggcaaaagcucgcuugaucuugauuuucaguacg
aauacagaccgugaaagcggggccucacgauccuucugaccuuuuggguu
uuaagcaggaggugucagaaaaguuaccacagggauaacuggcuuguggc
ggccaagcguucauagcgacgucgcuuuuugauccuucgaugucggcucu
uccuaucauugugaagcagaauucaccaagcguuggauuguucacccacu
aauagggaacgugagcuggguuuagaccgucgugagacagguuaguuuua
cccuacugaugauguguuguugccaugguaauccugcucaguacgagagg
aaccgcagguucagacauuugguguaugugcuuggcugaggagccaaugg
ggcgaagcuaccaucugugggauuaugacugaacgccucuaagucagaau
cccgcccaggcggaacgauacggcagcgccgaggagccucgguuggcccc
ggauagccggguccccguccguccccgcucccgggcgucgggaccggggu
ccggugcggagagccguucgucuugggaaacggggugcggccggaaaggg
ggccgcccucucgcccgucacguugacgcacguucguguggaaccuggcg
cuaaaccauucguagacgaccugcuucugggucgggguuucguacguagc
agagcagcucccucgcugcgaucuauugaaagucagcccucgacacaagg
guuugu

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>>..>>>>>>>>>...>>>>>>>>>.......<<<<<<<........>>>
>>>>.....(((((..<<<<<<)))))..]]]..................
................<<....>>...............<<<......>>
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>>>>>>>......>.>.>>>.>>>>>...<<<<<<<.<<<<<<<<<<<<<
<<...<<.<<<<.<<..<<<......<<<<<<<<.<....>.>>>>>>>>
........>>>>>>>>>.......>>>>>>..>>>>>>>>>>>.......
........<<<<<<.<<<<..<<..>>..>>>>...<<<.<<<.<.<<<<
<<<<<<<<<<<<<..<<<<<<..............>>>>>>.>>>>..>>
>>>>>>>>.>>>....>>>..>>>>.....<<.....>>.....>>>>>>
......>>.>>>>>...>>>>>...>>>>>>>>..<<<<........>>>
><<<<<<....<<<<<<<<<<<<<..<<<...<<<<........>>>>..
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<<..<<<...(((.[...>>>..>>>....>>>>>.<<<<<<<<<.<<<<
......>>>>>>>>>>>.>>....>>>>>>>>>>>>>..<<.<..<<<<<
<<.....<<<<<<<<<..<<<<...<<<<<<<....>>>>>>>...>.>>
>...<<<..<<.<<........>>>>.>>>....>>>>>>>>>...<<..
.....>>......>>>>>>...>..>.>>..<<<<<<....<.<<<....
>>>.><<<<<<<<<<.......<<<.<<<<<<<<<...<<<....<<<..
<.<<<<...<<<<.<<...>>.>>>>.>>>>.>..>>>...>>>...>>>
>>>>...<<<<<<<<<.<......>>>>>>>>>>....>>>>>>>>>>>>
.>>>..<<<<.<<<<<.....>>>>>.>>>>....<<<......>>>...
<<<<<<<...<<<(((.......<<<<<.....<<..........<<<.<
<<<<<<<<<.......<<<<<.<<<...<<.........>>...>>>...
......<<......>>...>>>>>...>>>>>>>>>>>>>..........
))).>>...>>>>>..>>>...>>>>>>><<<<<<.<<<...........
..].)))...<<.<<.>>>>..................>>>>>>>>>...
.>>>>>>...<<<<<<<..<<......<<<<.<<<<....>>>>>>>>..
.>>.>>>>>>>........>>>>>><<.....<<.....<<<<<<.....
..>>>>>>.<<<.....<<<<<.<<...<<<<<<...<<.....>>.>>>
>>>....((<<<<<<...<<....<<<....>>>..........<<.<<<
<<<<<<...>>>>>>>>>>>......>>....>>>..<<<<<........
>>>>>))<<<<....<<<<<...<<<<<<<....>>>>>>>...<<<...
.>>>...<..<<<<<<<...<<<<<<<<<<<<<.<<<<<<<<<..{{{..
>>>>>>....<<<<<.<<<<.<<<......<<<.....>>>......>>>
>>>>..>>>>>.....<<<<<<<<<..>>.>>>>>>>>>>..>.>>>>>>
>>>>>>.........>>>>>>>..>..........>>.>>>.>>>>.>>>
..>>.....<<......>>>>>>>.<<<<.....>>>>..>>>....<<<
<.....<<<<<<<.<<.........<.....<<<<<....<<..>>....
.>>>>><<.<<<<<<<<<<<..<<<.<<..>>.>>>.......>>>>>..
>>>>>>.>>............<<<<<<<<<..<<<.........>>>..<
<<<<<<....<<<<.....>>>>......>>>>>>>...<<..<<<..<<
<<<.<<..<<<.....<<<<....>>>>.....>>>>>>>>..>>>>><<
<<<<.......>>>>>>........<...........<<<<<.......>
>>>>.....>.....>>....>>.>>>>>>>..>..>>.>>>>>>>..>>
>>>>....>>....<<<<<........>>>>>....[[[[[..{{{.{{.
.....[[[[.{{[[[[..<<<<<..<<<<<<<<.<<<<<<<<<<<<<<<<
<...........<<<<..<<<<.>>>>............>>>>..>>>>>
>>.>>>>>>>>>>...<<<<<.....<<<.<..<.<<<<.<<...>>>>>
>.>..............<<<<<<<.<<<<<....>>>>>>>>>>>>..>>
>>..>>>>>....>>>>>>>>...>>>>>...<<<.....>>>..<<<<<
<...........>>>>>>..<<.....<<<<<...<<<<<<.......>>
>>>>.....<<...(((>>....>>>>>.....<<<.<<<<<.......>
>>>.>>>>.<<<....>>>.>>))).......((((((..(((..>>>>>
>.)))))))))..........]]]]..}}]]]].......<<.<.<.<<<
<<<...<<.......>>...>>>>>>...>>.>.>.......<<<<<.<<
<<<<..<.<.<<<<<......>>>>>...>.>.<<<.........>>>..
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.}}}.]]]]].....<<<<<<....<<<..<.<<<<<.............
....>>>>>.><<<<....<<<<....<<.<<<<<<<}}}.>>>>>..>>
.>>...>>>>...>>>><<<<<...<<<<<...........>>>>>...>
>>>>.>>>.....>>>>>>.....<<<<<<.<<<..<<.<....<<<...
.....<<<<<<.<<<<<..<..<.<.........>.>....>..>>>>>>
>>>>>.......>>>...<<<..<<<<......<<.<<<<<<........
>>>>>>>>.....>>>>..>>>...............>.>>..>>>>>>>
>><<<<<.<.............>....<<.<<<<<<<.<<.....<<<<.
...<<<<<......>>>>>...>>>>....>>....>>>>.>>>.>>..>
>>>>..

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7ls2 Functionally distinct roles for eEF2K in the control of ribosome availability and p-body abundance.
• Resolution: 3.1 Å
• Binding residue (original residue number in PDB): V2 N3 P5 R8 R9 F11 K17 H18 Y26 K28 G29 K30 S32 Y34 A35 Q36 G37 R39 R40 R43 K44 G47 Y48 G50 Q51 T52 K53 P54 I55 F56 K58 A60 K61 T63 K64 K65 K80 R81 A84 K86 C88 K89 K97 K98 K100
• Binding residue (residue number reindexed from 1): V1 N2 P4 R7 R8 F10 K16 H17 Y25 K27 G28 K29 S31 Y33 A34 Q35 G36 R38 R39 R42 K43 G46 Y47 G49 Q50 T51 K52 P53 I54 F55 K57 A59 K60 T62 K63 K64 K79 R80 A83 K85 C87 K88 K96 K97 K99

Gene Ontology

Molecular Function

• GO:0003674 molecular_function
• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0002181 cytoplasmic translation
• GO:0006412 translation
• GO:0008150 biological_process
• GO:0140236 translation at presynapse
• GO:0140242 translation at postsynapse

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:0045202 synapse
• GO:0098793 presynapse
• GO:0098794 postsynapse
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:7ls2, PDBe:7ls2, PDBj:7ls2
• PDBsum: 7ls2
• PubMed: 34815424
• UniProt: P83882|RL36A_MOUSE Large ribosomal subunit protein eL42 (Gene Name=Rpl36a)