Structure of PDB 6fai Chain i Binding Site BS01

Receptor Information

>6fai Chain i (length=262) Species: 559292 (Saccharomyces cerevisiae S288C)

ALPEKVIKAYTTVGSILKTWTHGKLPKLFKVIPSLRNWQDVIYVTNPEEW
SPHVVYEATKLFVSNLTAKESQKFINLILLERFRDNIETSEDHSLNYHIY
RAVKKSLYKPSAFFKGFLFPLVETGCNVREATIAGSVLAKVSVPALHSSA
ALSYLLRLPFSPPTTVFIKILLDKKYALPYQTVDDCVYYFMRFRILDDGS
NGEDATRVLPVIWHKAFLTFAQRYKNDITQDQRDFLLETVRQRGHKDIGP
EIRRELLAGASR

Ligand information

>6fai Chain 2 (length=1775)

aucugguugauccugccaguagucauaugcuugucucaaagauuaagcca
ugcaugucuaaguauaagcaauuuauacagugaaacugcgaauggcucau
uaaaucaguuaucguuuauuugauaguuccuuuacuacaugguauaacug
ugguaauucuagagcuaauacaugcuuaaaaucucgacccuuuggaagag
auguauuuauuagauaaaaaaucaaugucuucggacucuuugaugauuca
uaauaacuuuucgaaucgcauggccuugugcuggcgaugguucauucaaa
uuucugcccuaucaacuuucgaugguaggauaguggccuaccaugguuuc
aacggguaacggggaauaaggguucgauuccggagagggagccugagaaa
cggcuaccacauccaaggaaggcagcaggcgcgcaaauuacccaauccua
auucagggagguagugacaauaaauaacgauacagggcccauucgggucu
uguaauuggaaugaguacaauguaaauaccuuaacgaggaacaauuggag
ggcaagucuggugccagcagccgcgguaauuccagcuccaauagcguaua
uuaaaguuguugcaguuaaaaagcucguaguugaacuuugggcccgguug
gccggucggauuuccaacggggccuuuccuucuggcuaaccuugaguccu
uguggcucuuggcgaaccaggacuuuuacuuugaaaaaauuagaguguuc
aaagcaggcguauugcucgaauauauuagcauggaauaauagaauaggac
guuugguucuauuuuguugguuucuaggaccaucguaaugauuaauaggg
acggucgggggcaucaguauucaauugucagaggugaaauucuuggauuu
auugaagacuaacuacugcgaaagcauuugccaaggacguuuucauuaau
caagaacgaaaguuaggggaucgaagaugaucagauaccgucguagucuu
aaccauaaacuaugccgacuagggaucgggugguguuuuuuuaaugaccc
acucggcaccuuacgagaaaucaaagucuuuggguucuggggggaguaug
gucgcaaggcugaaacuuaaaggaauugacggaagggcaccaccaggagu
ggagccugcggcuuaauuugacggggaaacucaccagguccagacacaau
aaggauugacagauugagagcucuuucuugauuuugugggugguggugca
uggccguucuuaguugguggagugauuugucugcuuaauugcgauaacga
acgagaccuuaaccuacuaaauaguggugcuagcauuugcugguuaucca
cuucuuagagggacuaucgguuucaagccgauggaaguuugaggcaauaa
caggucugugaugcccuuagacguucugggccgcacgcgcgcuacacuga
cggagccagcgagucuaaccuuggccgagaggucuugguaaucuugugaa
acuccgucgugcuggggauagagcauuguaauuauugcucuucaacgagg
aauuccuaguaagcgcaagucaucagcuugcguugauuacgucccugccc
uuuguacacaccgcccgucgcuaguaccgauugaauggcuuagugaggcc
ucaggaucugcuuagagaagggggcaacuccaucucagagcggagaauuu
ggacaaacuuggucauuuagaggaacuaaaagucguaacaagguuuccgu
aggugaaccugcggaaggaucauua

..<<<<<...[[[[>>>>>[[[[.((((((................[[[.
[[[..<<....<<....<<..........>>...>>.>>......{{...
.....[[[..{{..{{....[[[..................<.....<<.
<<.......>>.>>......>.........<<<<<..<<....>>..>>>
>>....((((((......<<<<<<<<<<<<..>>>>....<<......>>
)))))).........<<<<..<<<.....>>>.>>>>....]]]...}}}
}..]]].<<<....<<<....<<<<<<<<.......>>>>>>>>>>>...
...>>>...<<<.<<<<....>>>>....>>>.}}.<<.<<<........
..>>>.>>.<.<<....>>.>...]]]]]].........<<<....<<<.
....>>>..>>>...............<<<<<<<<<<<<......>>>>>
>>>>.>>>......<<..<...........>..>>.........<<<<<(
(......<<<<.........)).........>>>>.>>>>>..))))))]
]]].........[[[{{.......{{...[[[.{{....<<<<<<.<<<<
<....<..>....>>>>>.>>>>>>.....<<<<<.<..<<..<<<<<..
...>>>>>..>>..>.>>>>>....<<<<<.<<.......<<...<....
...>..<<<.....>>>....>>......>>.>>..>>>.........[[
[..((((((((....>>>>>>>>.)))))))).]]].....}}....<<<
<<<.<<...<<<<..<<..<<<<<<.<...<<<......>>>......>.
>>>>>>..>>......<<<....>>>..>>>>...>>>>>.>>>...]]]
...}}......<<<<<<<...<...<<<<.........>>>>...>>>>>
>>>..........<<<.<<.<<<..<<<<<<<<.<<<........>>>>>
>>>>>>..>>>...<<..}}>>...>>.....>>>.]]].<<<......<
<<<....>>>>....>>>..]]]]....<<<.<<<<<<<..<<.<<<<<<
..<<<.<<<<<<......<<.>>..((......<<<<<.<....<<<<<.
.......<<.<<<........>>>.>>......>>>>>......<<<.<<
<<<<<<<<....<<<.<<<<<....>>>...<<<......>>>...>>.>
>>....<<<<..<<..<<<<..<<<<<.<<<<<<....>>>>>>...>>>
>>..>>>>.>>....<<<<<<.....>>>>>>........>>>>....>>
>.>>>>.>>>..>>>.......>.>>>>>...>>.>>>>.>>>.....<<
<<<<<......<<.....<<..<<<<....>>>>..>>....>>......
.>>>>>>>......<....<<<<<<..........>>>>>>....>....
.>>>>>>....<<<<<<<<...))..>>>>>>>>......>>...>>>>>
>>>>>....<<<...<<.<..<<<<.<<..<<<<<<<<<<.<<<..<.<<
..<...<<<<<<<.<<<<..<<<....>>.>.>>>>.>>>>>>>...>..
>>.>..>>>.>>>>>>>>>>.>>.>>>>...>.>>......>>><<<<<<
<<<....>>>>>>>>>.........

Receptor-Ligand Complex Structure

Structure summary

• PDB: 6fai Structure of a eukaryotic cytoplasmic pre-40S ribosomal subunit.
• Resolution: 3.4 Å
• Binding residue (original residue number in PDB): T225 H226 D296 S298 H302 Y304 R333 I337 S340 A343 K344 V345 S346 K419 H449 I452
• Binding residue (residue number reindexed from 1): T21 H22 D92 S94 H98 Y100 R129 I133 S136 A139 K140 V141 S142 K215 H245 I248

Gene Ontology

Molecular Function

• GO:0005515 protein binding
• GO:0030515 snoRNA binding

Biological Process

• GO:0000447 endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
• GO:0006364 rRNA processing
• GO:0016973 poly(A)+ mRNA export from nucleus
• GO:0030490 maturation of SSU-rRNA
• GO:0042254 ribosome biogenesis

Cellular Component

• GO:0005634 nucleus
• GO:0005730 nucleolus
• GO:0005737 cytoplasm
• GO:0030686 90S preribosome
• GO:0030688 preribosome, small subunit precursor
• GO:0032040 small-subunit processome

External links

• PDB: RCSB:6fai, PDBe:6fai, PDBj:6fai
• PDBsum: 6fai
• PubMed: 29459436
• UniProt: P38333|ENP1_YEAST Essential nuclear protein 1 (Gene Name=ENP1)