Structure of PDB 6em3 Chain i Binding Site BS01

Receptor Information

>6em3 Chain i (length=74) Species: 559292 (Saccharomyces cerevisiae S288C)

SYKKGAASNRTKFVRSLVREIAGLSPYERRLIDLIRNSGEKRARKVAKKR
LGSFTRAKAKVEEMNNIIAASRRH

Ligand information

>6em3 Chain 1 (length=1250)

guuugaccucaaaucagguaggaguacccgcugaacuuaagcauaucaau
aagcggaggaaaagaaaccaaccgggauugccuuaguaacggcgagugaa
gcggcaaaagcucaaauuugaaaucugguaccuucggugcccgaguugua
auuuggagagggcaacuuuggggccguuccuugucuauguuccuuggaac
aggacgucauagagggugagaaucccguguggcgaggagugcgguucuuu
guaaagugccuucgaagagucgaguuguuugggaaugcagcucuaagugg
gugguuccaucuaaagcuaaauauuggcgagagaccgauagcgaacaagu
acagugauggaaagaugaaaagaacuuugaaaagagagugaaaaaguacg
ugaaauuguugaaagggaagggcauuugaucagacaugguguuuugugcc
cucugcuccuuguggguaggggaaucucgcauuucacugggccagcauca
guuuugguggcaggauaaauccauaggaauguagcuugccucgguaagua
uuauagccugugggaauacugccagcugggacugaggacugcgacguaag
ucaaggaugcuggcauaaugguuauaugccgcccgucuugaaacacggac
caaggagucuaacgucuaugcgaguguuuggguguaaaacccauacgcgu
aaugaaagugaacguagguuggggccucgcaagaggugcacaaucgaccg
auccugaggauggauuugaguaagagcauagcuguugggacccgaaagau
ggugaaaaccaucuaguagcugguuccugccgaaguuucccucaggauag
cagaagcucguaucaguuuuaugaauguaagaaugaagagcuuuuagugg
gccauuuuugguaagcagaacuggcgaugcgggaugaaccgaacguagag
uuaaggugccggaauacacgcucaucagauggcgcucaagcguguuaccu
auacucuaccgucaggguugauaugaugcccugacgaguaggcaggcgug
gaggucagugacgaagccuagaccguaaggucgggucgaacggccucuag
ugcagaucuuggugguaguagcaagcgaaaccacagccaagggaacgggc
uuggcagagcuccacacagauggauacgaauaaggcguccuuguggcguc
gcugaaccauagcaggcuagcugcuggcgaauugcaaugcguggggauaa

..........................<<<<<<..................
.>>>>>.>...<.....<<....>>..<<<<<......<<.....>>...
..>>>>>..<<<...........<<.<<<<<<...>>>>>>.>>......
.......<<<<<<<.<<<<<<<<<<<<<<<<<<<<<....<<<<......
>>>>.(.......<<<......)>>>..>.>>>>>>>>..>>>>>>>>..
..>>>>>>>>>>>........<<<<<<<........>>>>>>>.....<<
<<<<..>>>>>>..>>>....>............................
.<<....>>...............<<<......>>>..............
....................<<<<<.<<<<<<.....<<<<...<<<..<
<<<<<<<<.....>>>>>>>>>......>>>...>>>>..<<<<<<<.<<
<<<<<<<<<<<<<.....<<<<<<<<<....<.<<<<<......>>>>>>
......>>>>>>>>..>.>>>>>.>>>>>>>>>>........<<<....>
>>.....>>>>>>>....>>>>>>.>>>>>..<<<<........>>>><<
<<<<....<<<<<<.<<<<<<..<<<...<<<<.....>>>>...>>>..
.<<....>>.......<<<<<..<<<<<....>>>>>...>>>>>.<<<<
<<<<.......>>>.>>>.>>....>>>>>>.>>>>>>..<<.<...<<<
<<<....>>>>>>......>.>>..<<<<<<......<<......>>..<
<<<<<<<<....<<<.<<<<<<<..>>>>>.>>>>>..>>>>>>>.>>..
<<<<.<<<<<.....>>>>>.>>>>....<<<......>>>...<<<<<<
<..<<<<(((.....<<<<<<......<..))).>....>>>>>>.>>>>
..>>>>>>><<<<<<<<<.........>>>>>>>>>....>>>>>><.<<
<<<<<<.<<......<<<<.<<<<....>>>>>>>>...>>>>>>>>>>.
.>.....>>>>>><<.....<<..>>....>>....<<<<<........>
>>>>.....<<<<<<......<<..........<<<<<<.....>>>>>>
......>>.......<<<<<<.>>>>>>............>>>>>>....

Receptor-Ligand Complex Structure

Structure summary

• PDB: 6em3 Visualizing the Assembly Pathway of Nucleolar Pre-60S Ribosomes.
• Resolution: 3.2 Å
• Binding residue (original residue number in PDB): G31 A32 A33 R76 G78
• Binding residue (residue number reindexed from 1): G5 A6 A7 R50 G52

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003735 structural constituent of ribosome
• GO:0005515 protein binding

Biological Process

• GO:0002181 cytoplasmic translation
• GO:0006412 translation

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:6em3, PDBe:6em3, PDBj:6em3
• PDBsum: 6em3
• PubMed: 29245012
• UniProt: O14455|RL36B_YEAST Large ribosomal subunit protein eL36B (Gene Name=RPL36B)