Structure of PDB 6hcj Chain h3 Binding Site BS01

Receptor Information

>6hcj Chain h3 (length=122) Species: 9986 (Oryctolagus cuniculus)

AKIKARDLRGKKKEELLKQLDDLKVELSQLRVAKVTGGAASKLSKIRVVR
KSIARVLTVINQTQKENLRKFYKGKKYKPLDLRPKKTRAMRRRLNKHEES
LKTKKQQRKERLYPLRKYAVKA

Ligand information

>6hcj Chain 51 (length=3635)

cgcgaccucagaucagacguggcgacccgcugaauuuaagcauauuaguc
agcggaggaaaagaaacuaaccaggauucccucaguaacggcgagugaac
agggaagagcccagcgccgaauccccgccccgcggcggggcgcgggaaau
guggcguacggaagacccacuccccggcgccggggggcccaaguccuucu
gaucgaggcccagcccguggacggugugaggccgguagcggcccccggcg
cgccgggcccgggucuucccggagucggguugcuugggaaugcagcccaa
agcgggugguaaacuccaucuaaggcuaaauaccggcacgagaccgauag
ucaacaaguaccguaagggaaaguugaaaagaacuuugaagagagaguuc
aagagggcgugaaaccguuaagagguaaacgggugggguccgcgcagucc
gcccggaggauucaacccggcggcgcgguccggccgugccggcggggcgg
aucucccgcucccgucggcgggggaccgccccccggccggcgaccggccg
ccgccgggcgcauuuccaccgcggcggugcgccgcgaccggcuccgggac
ggcugggaaggccccggcggggaagguggcccgggggccccggguguuac
agccccgccccggcagcagcagcucgccgaaucccggggccgagggagcg
agacccgugcgccgcgcucuccccccucgggggggccgggccaccccucc
cacggcgcgaccgcucucccacccggggggcggggcggacuguccccagu
gcgccccgggcgggucgcgccgucgggcccgggggagguucucucggggc
cacgcgucccccgcgagcgcacggggucggcggcgaugucggcuacccac
ccgacccgucuugaaacacggaccaaggagucuaacacgugcgcgagucg
ggggcuacgaaagccgccguggcgcaaugaaggugaaggccggcggcgcg
ccgccggccgaggugggaucccgaggccuuccaguccgccgagggcgcac
caccggcccgucucgcccgccgcgccggggagguggagcacgagcgcacg
uguuaggacccgaaagauggugaacuaugccugggcagggcgaagccaga
ggaaacucugguggagguccguagcgguccugacgugcgaaucggucguc
cgaccuggguauaggggcgaaagacuaaucgaaccaucuaguagcugguu
cccuccaaaguuucccucaggauagcuggcgcucuccgcaguuuuauccg
guaaagcgaaugauuagaggucuuggggccgaaacgaucucaaccuauuc
ucaaacuuuaaauggguaagaagcccggcucgcugguguggagccgggcg
uggaaugcgagugccuagugggccacuuuugguaagcagaacuggcgcug
cgggaugaaccgaacgccggguuaaggcgcccgaugccgacgcucaucag
accccagaaaagguguugguugauauagacagcaggaugguggccaugga
agucggaauccgcuaaggaguguguaacaacucaccugccgaaucaacua
gcccugaaaauggauggcgcuggagcgucgggcccauacccggccgucgc
uggcagucggcagagcgagaaagagaguggcgcggacgcuacgccgcgac
gaguaggagggccgcugcggugagccuugaagccuagggcgcgggcccgg
guggagccgccgcaggugcagaucuuggugguaguagcaaauauucaaac
gagaacuuugaaggccgaaguggagaaggguuccaugugaacagcaguug
aacaugggucagucgguccugagagaugggcgagcgccguuccgaaggga
cgggcgauggccuccguugcccucagccgaucgaaagggagucggguuca
gauccccgaauccggaguggcggagaugggcgccgcgaggcguccagugc
gguaacgcgaccgaucccggagaagccggcgggagccccggggagaguuc
ucuuuucuuugugaagggcagggcgcccuggaauggguucgccccgagag
aggggcccgugccuuggaaagcgucgcgguuccggcggcguccggugagc
ucucgcuggcccuugaaaauccgggggagaggguguaaaucucgcgccgg
gccguacccauauccgcagcaggucuccaaggugaacagccucuggcaug
uuggaacaauguagguaagggaagucggcaagccggauccguaacuucgg
gauaaggauuggcucuaagggcuggcagccgacuuagaacuggugcggac
caggggaauccgacuguuuaauuaaaacaaagcaucgcgaaggcccgcgg
cggguguugacgcgaugugauuucugcccagugcucugaaugucaaagug
aagaaauucaaugaagcgcggguaaacggcgggaguaacuaugacucucu
uaagguagccaaaugccucgucaucuaauuagugacgcgcaugaauggau
gaacgagauucccacugucccuaccuacuauccagcgaaaccacagccaa
gggaacgggcuuggcggaaucagcggggaaagaagacccuguugagcuug
acucuagucuggcacggugaagagacaugagagguguagaauaaguggga
ggcccgggccgccggugaaauaccacuacucugaucguuuuuucacugac
ccggugaggcgggggggcgagccgggcucucgcuucuggcgccaagcgcc
cggccggccgggcgcgacccgcuccggggacagugccagguggggaguuu
gacuggggcgguacaccugucaaacgguaacgcagguguccuaaggcgag
cucagggaggacagaaaccucccguggagcagaagggcaaaagcucgcuu
gaucuugauuuucaguacgaauacagaccgugaaagcggggccucacgau
ccuucugaccuuuuggguuuuaagcaggaggugucagaaaaguuaccaca
gggauaacuggcuuguggcggccaagcguucauagcgacgucgcuuuuug
auccuucgaugucggcucuuccuaucauugugaagcagaauucaccaagc
guuggauuguucacccacuaauagggaacgugagcuggguuuagaccguc
gugagacagguuaguuuuacccuacugaugauguguuguugccaugguaa
uccugcucaguacgagaggaaccgcagguucagacauuugguguaugugc
uuggcugaggagccaauggggcgaagcuaccaucugugggauuaugacug
aacgccucuaagucagaaucccgcccaggcggaacgauagcgccggagcc
ucgguuggccucggauagccggucccccgcccgggguccggugcggagag
cccuucgucccgggaaacggggcggccggaaagggggccgcccucucgcc
cgucacgcaccgcacguucguggggaaccuggcgcuaaaccauucguaga
cgaccugcuucugggucgggguuucguacguagcagagcagcugcugcga
ucuauugaaagucagcccucgacacaaggguuugu

.........................<<<<<<.....<......>......
>>>>>.>.........<......>..<<<<<<.....<<.....>>....
>>>>>>..<<<..<.<<<...<<<<<<<<<<<...>>>>>>>.>>>>...
..>>>.>..<<<.<<<<<....<<<..<<<..<<<<<<<<....<<<<..
....>>>>.............<<<.......>>>...>.>>>>>>>...>
>>..>>>...>>>>.>>>>.......<<<<<<<........>>>>>>>..
....<<<<..(((((.>>>>...>>>........................
..........<<....>>...............<<<......>>>.....
.............................<<<<<<<<..<<..<....<<
<<<<<<.........<<<<<<<<<<....<<<<<<<.<<....<<<<<<<
.<<<<<<<<<......>>>>>>>>>>>>>>>>...>>>>>>...>>>.>>
>>>>>>>>..........>>>.>>>>>...<<<<<<<.<<<<<<<<<<.<
<<<.........<..<<<<<<.......<<<<.<<<.>>>.>>>>.....
...>>>>>>...>............>>>>...>>>>>>>>>>.....<<.
......>>.............<<<<<<..>>>>>>...<.<<.<<<<.<<
<<<<<...<<<<<<....<..<<...>>>.<<<<<<<...<<....>>..
.>>>>>>>.>>.>>>>...>>>>.>>>...>.>>>...........>>.>
..........................>>.>>>>>....>.>>...>>>>>
>>>..<<<<........>>>><<<<<<....<<<<<<<<<<<<<..<<<.
..<<<.......>>>.....>>>...<<....>>...<<<<<<<<<<..>
>>>>>>>>>..<<<<<...<<<..<<........[...>>..>>>....>
>>>>.<<<<<<<<<.<<<<......>>>>>>>>>>>.>>....>>>>>>>
>>>>>>..<<.<..<<<<<<<.....<<<<<<<<<...<<.......<<.
<.....>>>........>>....<<<..<..<<........>>.>.>>>.
...>>>>>>>>>...<<.......>>......>>>>>>...>..>.>>..
<<<<<<....<.<<<....>>>.><<<<<<<<<<...<<<.<<<<<<<<.
...<<<....<<<...<<<<..<<<.<<.........>.>>>>>>>.>..
>>>...>>>....>>>>>>...<<<<<<<<<.<......>>>>>>>>>>.
...>>>>>>>>>>>>.>>>..<<<<.<<<<<.....>>>>>.>>>>....
<<<......>>>...<<<<<<<...<<.(((.......<<<<<.....<<
..........<<<.<<<..<<<.....................<<.....
....>>...............<<......>>.............>>>..>
>>>>>..........))).>>...>>>>>...>>...>>>>>>><<<<<.
.<<<.....................]................>>>.>>>>
>....>>>>>>..<<<<<<<<..<<......<<<<.<<<<....>>>>>>
>>...>>.>>>>>>>.>......>>>>>><<<....<<.....<<<<<<.
......>>>>>>.<<......<<.<..<<...<<<<<<...<<.....>>
.>>>>>>....(.<<<<<<...<<....<<<....>>>............
.<<<<<.<<<...>>>.>>>>>........>>....>>>...<<<<....
....>>>>..)<<<<....<<<......<<<<<......>>>>>....<<
<....>>>......<<<<<<<...<<<<<<<<<<<<<<<<<<<<<<<..[
[[..>>>>>>.....<<<..<<<<.<<<<.....<<<.....>>>.....
>>>>>>>>...>>>......<<<<<<<.......>>>>>>>>>>>.>.>>
>>>>>>>>>>.........>>>>>>>................>>>.>>>>
.>>>..>>.....<<......>>.>.>>.<<<<.....>>>>...>>...
.<<<<.....<<<<<<..<<...............<<<<<..........
.....>>>>><<.<<<<<<<<<<<.>>>>>..>>>>>>.>>.........
...<<<<<<<<<..<<<.........>>>..<<<<<<<....<<<<....
.>>>>......>>>>>>>..<.<..<<<..<<<<<.....<<<......<
<<....>>>......>>>..>>>..>>>>><..<<.........>>...>
.......<...........<<<<<.......>>>>>.....>.....>..
>..>>.>>>>>>>.....>>..>>>>>>..>>>>>>...>>>....<<<<
<........>>>>>.....<<<<..<<<.<<......<<<<....<<<..
<<<<..<<<<<<<<..<<<.<<<<...<.<<<<..........<<<<<..
.<<<..>>>.............>>>>>.>>>>.>...>.>>.>>>>...<
<<<<.....<<<<.................................<<<<
<.<<..>>.>>>>>...>>>>..>>>>>.....>>>>>>>>...>>>>..
..<<.....>>...<<<<<<...........>>>>>>..<<...<<<<<<
<...<<<<<<.......>>>>>>.....<<.[.(((>>....>>>>>>>.
..<<.<<<<<<.......>>>>.>>>>.<<<....>>>.>>)))].....
.<<<<<<..<<<...))))).>>>>>>>>>..........>>>.....>>
>>.......<<..<<.<<<<<<...<<.......>>...>>>>>>...>>
.>.>.........<<<.<.<<<...<...<<<<<......>>>>>.....
>.<<<.........>>>......>>>...>...>>>.......<<<<<<<
....>>>.>>>>....>>..>>>.>>>>......<.<<<<....<<<..<
.<<<<<.................>>>>>.><<<<....<<<<....<<..
<.<<<.]]]..>>>....>.>>...>>>>...>>>><<<.....<<<<..
...........>>>>.....>>>.>>>.....>>>>.>.<<<<<<<..<<
<.....<<.........<<<<<....<<<..>>>...>>>>>........
>>...<<<..<<<<....<<<.<<<<<........>>>>>..>>>...>>
>>..>>>...............>>>..>>.>>>>>.<<<<<.<.......
......>....<<.<<<<<<<.<<.....<<<<.<..<<<<<.>>>>>..
>>>>>....>>....>>>>.>>>.>>..>>>>>..

Receptor-Ligand Complex Structure

Structure summary

• PDB: 6hcj ZNF598 Is a Quality Control Sensor of Collided Ribosomes.
• Resolution: 3.8 Å
• Binding residue (original residue number in PDB): R70 Y78 K79 K87 R89 R93 R94 N96 K97 H98 S101 K106 R109 R112
• Binding residue (residue number reindexed from 1): R69 Y77 K78 K86 R88 R92 R93 N95 K96 H97 S100 K105 R108 R111

Gene Ontology

Molecular Function

• GO:0003729 mRNA binding
• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0000463 maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
• GO:0006412 translation

Cellular Component

• GO:0005737 cytoplasm
• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:6hcj, PDBe:6hcj, PDBj:6hcj
• PDBsum: 6hcj
• PubMed: 30293783
• UniProt: G1SIT5|RL35_RABIT Large ribosomal subunit protein uL29 (Gene Name=RPL35)