Structure of PDB 7r81 Chain h2 Binding Site BS01

Receptor Information

>7r81 Chain h2 (length=310) Species: 5141 (Neurospora crassa)

EQLILKGTLEGHNGWVTSLATSLENPNMLLSGSRDKSLIIWNLTRDETSY
GYPKRRLHGHSHIVSDCVISSDGAYALSASWDKTLRLWELSTGTTTRRFV
GHTNDVLSVSFSADNRQIVSGSRDRTIKLWNTLGDCKFTITEKGHTEWVS
CVRFSPNPQNPVIVSSGWDKLVKVWELSSCKLQTDHIGHTGYINAVTISP
DGSLCASGGKDGTTMLWDLNESKHLYSLNANDEIHALVFSPNRYWLCAAT
SSSIIIFDLEKKSKVDELKPEFQNIGKKSREPECVSLAWSADGQTLFAGY
TDNIIRAWGV

Ligand information

>7r81 Chain A2 (length=1769)

uaccugguugauucugccaguagucauaugcuugucucaaagauuaagcc
augcaugucuaaguuuaagcaauuaaaccgcgaaacugcgaauggcucau
uaaaucaguuauaguuuauuugauaguaccuuacuacauggauaaccgug
guaauucuagagcuaauacaugcuaaaaaccccgacuucagggguguauu
uauuagauuaaaaaccaaugcccuucggggcuaacuggugauucauaaua
acuucucgaaucgcauggccuugcgcuggcgaugguucauucaaauuucu
gcccuaucaacuuucgacggcugggucuuggccagccauggugacaacgg
guaacggaggguuagggcucgaccccggagaaggagccugagaaacggcu
acuacauccaaggaaggcagcaggcgcgcaaauuacccaaucccgacacg
gggagguagugacaauaaauacugauacagggcucuuuugggucuuguaa
uuggaaugaguacaauuuaaaucccuuaacgaggaacaauuggagggcaa
gucuggugccagcagccgcgguaauuccagcuccaauagcguauauuaaa
guuguugagguuaaaaagcucguaguugaaccuugggcucggcccgucgg
uccugggucgggccuuuccuggagaaccgcaugcccuucacugggugugu
cggggaaccaggacuuuuaccgugaacaaaucagaucgcucaaagaaggc
cuaugcucgaauguacuagcauggaauaauagaauaggacgugugguucu
auuuuguugguuucuaggaccgccguaaugauuaauagggacagucgggg
gcaucaguauucaauugucagaggugaaauucuuggauuuauugaagacu
aacuacugcgaaagcauuugccaaggauguuuucauuaaucaggaacgaa
aguuaggggaucgaagacgaucagauaccgucguagucuuaaccauaaac
uaugccgauuagggaucggacgguguuauuuuuugacccguucggcaccu
uacgauaaaucaaaauguuugggcuccugggggaguauggucgcaaggcu
gaaacuuaaagaaauugacggaagggcaccaccagggguggagccugcgg
cuuaauuugacucaacacggggaaacucaccagguccagacacgaugagg
auugacagauugagagcucuuucuugauuucgugggugguggugcauggc
cguucuuaguugguggagugauuugucugcuuaauugcgauaacgaacga
gaccuuaaccugcuaaauagcccguauugcuuuggcaguacgcuggcuuc
uuagagggacuaucggcucaagccgauggaaguuugaggcaauaacaggu
cugugaugcccuuagauguucugggccgcacgcgcgcuacacugacacag
ccagcgaguacucccuuggccggaagguccggguaaucuuguuaaacugu
gucgugcuggggauagagcauugcaauuauugcucuucaacgaggaaucc
cuaguaagcgcaagucaucagcuugcguugauuacgucccugcccuuugu
acacaccgcccgucgcuacuaccgauugaauggcucagugaggcuuccgg
acuggcccagggaggucggcaacgaccacccagggccggaaagcuaucca
aacucggucauuuagaggaaguaaaagucguaacaaggucuccguuggug
aaccagcggagggaucauu

...<<<<<.[.((((>>>>><<<<.<<<<<<...<.<...<......<<<
.<<<..<<....<<<...<<.........>>..>>>.>>......<<...
.....<<<..<<..<<....<<<...............<<.....<..<<
<.....>>>..>......>>........<<<<<.......>>>>><...<
<<<<<......[.......<<<<....>>>>..............>>>>>
>.....>...<<<<..<<<.....>>>.>>>>....>>>...>>>>..>>
>.<<<....<<<......<<<<<<.......>>>>>>..>>>......>>
>...<<<.<<<<....>>>>....>>>.>>.<<.<<<..........>>>
.>>.<.<<<..>>>.>...>>>>>>.........<<<....<<<<...>>
>>..>>>..>....>.>....<<<<.<<<<<<..........>>>>>>..
>>>>.....<<..<...........>..>>.........<<<<<((....
..<<<<.....<<..))>>.......>>>>.>>>>>..>>>>>>>>>>..
.......<<<((.....<.<<...<<<.<<....<<<<<<<<<<<<....
...>>>>>>>>>>>>..<<<..............................
.........>>>....<<<<<<<<.......<<..<<.......>>.<<<
....>>>....>>......>>>>>..>>>.........<<<.<<<<<<<<
<...........].>>>>>>>>>>>>.....>>....<<<<<<.<<...<
<<<..<<..<<<<<<.<...<<<......>>>......>.>>>>>>..>>
.......<<....>>...>>>>...>>>>>.>>>...>>>...>>.>...
.<<<<<<<...<...<<<<.<.....>.>>>>...>>>>>>>>.......
...<<<.<<.<<<..<<<<<<<<.<<<<.....>>>>>>>>>>>>..>>>
...<<..))>>...>>.....>>>.>>>.<<<......<<<<....>>>>
....>>>..)))).]<<<<<.<<<<<<<..<<.<<<<<<..<<<..<<<.
.......<<........>>..........<<<<<<.....<<<<<.....
...<<.<<<........>>>.>>......>>>>>...<<.<<<..<<.<<
<<<<....<<<.<<<<<....>>>...<<<......>>>...>>.>>>..
..<<<<<.<<..<<<<..<<<<..<<<..........>>>....>>>>..
>>>>.>>....<<<<<<....>>>>>>.......>>>>>....>>>.>>>
.....>>>>>>>.....>>>.>>>......>>>..>>>.....<<<<<<<
......<<.....<<<.<<<<....>>>>.>>>....>>.......>>>>
>>>......<....<<<<<<..........>>>>>>....>.....>>>>
>>....<<<<<<<<.......>>>>>>>>......>>...>>>>>>>>>>
.>>....<..<<.<..<<<<.<<....<<<<<<<<.<<<..<<.<..<..
.<<<<<<<.<<<.<<<<<....>>>>>.>>>.>>>>>>>...>..>.>>.
.>>>.>>>>>>>>...>>.>>>>...>.>>...>.....<<<<<<<<<<.
.>>>>>>>>>>........

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7r81 Structure of the translating Neurospora ribosome arrested by cycloheximide
• Resolution: 2.7 Å
• Binding residue (original residue number in PDB): H60 G61 H64 R100 G278 K280 S281
• Binding residue (residue number reindexed from 1): H58 G59 H62 R98 G276 K278 S279

Gene Ontology

Molecular Function

• GO:0001965 G-protein alpha-subunit binding
• GO:0005080 protein kinase C binding
• GO:0005092 GDP-dissociation inhibitor activity
• GO:0030546 signaling receptor activator activity
• GO:0043022 ribosome binding
• GO:0045182 translation regulator activity

Biological Process

• GO:0006521 regulation of cellular amino acid metabolic process
• GO:0007186 G protein-coupled receptor signaling pathway
• GO:0010508 positive regulation of autophagy
• GO:0010629 negative regulation of gene expression
• GO:0017148 negative regulation of translation
• GO:0031139 positive regulation of conjugation with cellular fusion
• GO:0061157 mRNA destabilization
• GO:0070651 nonfunctional rRNA decay
• GO:0072344 rescue of stalled ribosome
• GO:0140469 GCN2-mediated signaling
• GO:1902660 negative regulation of glucose mediated signaling pathway
• GO:1903138 negative regulation of cell integrity MAPK cascade
• GO:1903753 negative regulation of p38MAPK cascade
• GO:1990116 ribosome-associated ubiquitin-dependent protein catabolic process
• GO:1990145 maintenance of translational fidelity
• GO:2000765 regulation of cytoplasmic translation
• GO:2001125 negative regulation of translational frameshifting

Cellular Component

• GO:0005634 nucleus
• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0022627 cytosolic small ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:7r81, PDBe:7r81, PDBj:7r81
• PDBsum: 7r81
• PubMed: 34815343
• UniProt: Q01369|GBLP_NEUCR Small ribosomal subunit protein RACK1 (Gene Name=cpc-2)