Structure of PDB 8ev3 Chain h Binding Site BS01

Receptor Information

>8ev3 Chain h (length=121) Species: 4896 (Schizosaccharomyces pombe)

ALKTFELRKQSQENLAEQLQELRQELASLRVQKIAGGSGSKLSKIKTTRK
DIARILTVINESNRLAAREAYKNKKYIPLDLRQKKTRAIRRALTPYEQSR
KTLKQIKKERYFPLRKYALKA

Ligand information

>8ev3 Chain 1 (length=1452)

uugaccucaaaucagguaggacuacgcgcugaacaauaagcgcaggaaaa
gaaaauaaccaugauucccucaguaacggugaagcgggaaaagcucaaau
uugaaaucuggcaacauuucuuuuguuguccgaguuguaauuucaagaag
cugcuuugaguguagacgaucggucuaaguuccuuggaacaggacgucag
agagggugagaaccccgucuuuggucgauuggauaugccauauaaagcgc
uuucgaagagucgaguuguuugcagcucuaaauggguggucaucuaaagc
uaaauauuggcgagagaccgauagcgaacaaguagagugaucgaaagaug
aaaagaacuuugaaaagagaguuaaauaguacgugaaauugcugaaaggg
aagcauuggaaaucagucuuaccugggugagaucaguagucucucgagac
uaugcacucugaaccugugguaggucagcaucaguuuucgggggcggaaa
aagaauaagggaagguggcuguuuauagcccuuguuguaauacguccacu
ggggacugaggacugcggcuugccaaggaugcugacauaaugguuuucaa
uggcccgucuugaaacacggaccaaggagucuagcaucuaugcgaguguu
ugggugaugaaaacccauccgcgaagugggaacgcccuuguggcgugcac
caucagagcauagcuguugggacccgaaagauggugaaccaucuaguagc
ugguuccugccgaaguuuccggauagcagaaacucagaucaguuuuauga
guauguaaaaugagaguuucuagugggccauuuuugguaagcagaacugg
cgaugcgggaugaaccgaacgugagguuaaggugccggaauguacgcuca
ucagacgaaaagguguuaguucauaugaacuagcccugggcgcuuaagcg
uacuacccauaccucaccgucuggguuagcuuugagaagcucagacgagu
aggcaggcguggagguuugugacgaagccuugggcgugagccugggucga
acagccucuagugcagaucuugguggaaguagcuagaucuagcgaaacca
cagccuggggaacgggccaggcucauaccgaagcagaauucgguaagcuc
gucgcaaugguaauucaacuuaguacgagaggaaccguugauucagauca
uugguauuugcggccgcggagcuaucaucugccggauaacggcugaacgc
cucuaagccagaauccgugccagaaagcgacgauuauguauaaaaauaga
gguaggcuacucuccuguaucguagaagaugggcgaugguugaugaaacg
gaaguguuuuauugacuuguccaugaaauuccaugaauccauugcauacg
acuuuaauguggaacgggguauuguagauuaagccuuuguucccaagauu
ug

........................<<<<<<........>>>>>.>.....
....<<....>>..<<<<<......<<.>>.....>>>>>..<<<..<<.
......<<.<<<<<<<.......>>>>>>>.>>.......>>....<<<<
<.<<<<<..<<<<<..<<<<<<<<<.....<<<<......>>>>.((...
...<<<.....))>>>......>>>>>>>>>..>>>>>.....>>>>>>>
>.>>........<<<<<<<..>>>>>>>.....<<<<<..>>>>>..>>>
..................................<<....>>........
.......<<<<....>>>>...............................
...<<<<<<<<<<<<.....<<<.<<<<.<<<.<<.<<<<<<<..>>>>>
>>>>...>>>..>>>>..>>>..<<<<<<<.<<<<<<<<<<<<<<<....
.....<<<<<<.................>>>>>>........>>>>>.>>
>>>>>>>>........<<<..>>>.....>>>>>>>....>>>>>>>>>>
>>..<<<<........>>>><<<<<<....<<<<<<.<<<<<<..<<<..
.<<<<.......>>>>...>>>...<<<<..<<<<<.....>>>>>...>
>>>....>>>>>>.>>>>>>..<<.<..<<<<<<<..>>>>>>>.....>
.>>..<<<<<<....<.<<..>>.><<<<<<<<<....<<<.<<<<<<<.
...>>>>>>>>>>.>>>>>>>.>>..<<<<.<<<<<.....>>>>>.>>>
>....<<<......>>>...<<<<<<<...<<<(((.....<<<<<<...
..<<......<<<.<<<<<<<<<..>>>>>>>>>>>>.))).>>...>>>
>>>.>>>...>>>>>>><<<<<<<<<............>>>>>>>>>...
.>>>>>>..<<<<<<<..<<......<<<<.<<<<....>>>>>>>>...
>>.>>>>>>>........>>>>>><<.....<<<<<..>>>>>....>>.
...<<<<<........>>>>>....<<<<<<<......>>>>>>>...<<
<<<<<..<<<<.<.<<<<<.................>>>>>.><<<<<..
..<<<....<<<..>>>...>>>...>>>>>.<<<<....<<<<......
.....>>>>....>>>>.>>>>....>>>>>>>......<<.......<<
<<..<..>..>>>>....>>........<<<<<....<<<<<<<<<<<<.
.....>>>>>>>>>>>>..>>>>>..........<<<<<.<.........
....>.....<<<<<<<<<.<<<...>>>....>>>>.>>>>>...>>>>
>.

Receptor-Ligand Complex Structure

Structure summary

• PDB: 8ev3 Chromatin localization of nucleophosmin organizes ribosome biogenesis.
• Resolution: 3.0 Å
• Binding residue (original residue number in PDB): R69 E70 K73 K76 Y77 I78 L82 R83 Q84 K86 R88 R92 T95 Y97 R101 K102 L104 K105 I107 K108 R111 Y112 F113 K117
• Binding residue (residue number reindexed from 1): R68 E69 K72 K75 Y76 I77 L81 R82 Q83 K85 R87 R91 T94 Y96 R100 K101 L103 K104 I106 K107 R110 Y111 F112 K116

Gene Ontology

Molecular Function

• GO:0003729 mRNA binding
• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0000463 maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
• GO:0002181 cytoplasmic translation
• GO:0006412 translation

Cellular Component

• GO:0005634 nucleus
• GO:0005730 nucleolus
• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:8ev3, PDBe:8ev3, PDBj:8ev3
• PDBsum: 8ev3
• PubMed: 36423630
• UniProt: O74904|RL35_SCHPO Large ribosomal subunit protein uL29 (Gene Name=rpl35)