Structure of PDB 8eth Chain h Binding Site BS01

Receptor Information

>8eth Chain h (length=116) Species: 4896 (Schizosaccharomyces pombe)

ALKTFELRKQSQENLAEQLQELRQELASLRVQKISKLSKIKTTRKDIARI
LTVINESNRLAAREAYKNKKYIPLDLRQKKTRAIRRALTPYEQSRKTLKQ
IKKERYFPLRKYALKA

Ligand information

>8eth Chain 1 (length=1303)

uugaccucaaaucagguaggacuacgcgcuaagcgcaggaaaagaaaaua
accaugauucccucaguaacgugaagcgggaaaagcucaaauuugaaauc
uggcaacauuucuuuuguuguccgaguuguaauuucaagaagcugcuuug
aguguagacgaucggucuaaguuccuuggaacaggacgucagagagggug
agaaccccgucuuuggucgauuggauaugccauauaaagcgcuuucgaag
agucgaguugugcagcucuaaauggguggucaucuaaagcuaaauauugg
cgagagaccgauagcgaacaaguagagugaucgaaagaugaaaagaacuu
ugaaaagagaguuaaauaguacgugaaauugcugaaagcauuggaaauca
gucuuaccugggugagaucaguagucucucgagacuaugcacucugaacc
ugugguaggucagcaucaguuuucgggggcggaaaaagaauaagggaagg
uggcuguuuauagcccuuguuguaauacguccacuggggacugaggacug
cggcuucgugccaaggaugcugacauaaugguuuucaauggcccguccac
ggaccaaggagucuagcaucuaugcgaguguuugggugaugaaaacccau
ccgcgaggugggaacgcccuggcgugcaccaucagagcauagcuguuggg
acccgaaagauggugaaccaucuaguagcugguuccugccgaagagcaga
aacucagaucaguuuuaaaugagaguuucuagugggccauuacuggcgau
gcgggccgaacgugagguuaaggugccggaauguacgcuagaaagguguu
aguuaacuagcccugggcgcuuaagcguacuacccauaccucaccgucug
gguuagcuuugagaagcucagacgaguaggcaggcguggagguuugugac
gaagccuugggcgugagccugggucgaacagccucuagugcagaucuugg
uggaaguagcagcgaaaccacagccugggggccaggucgucgcaauggua
auucaacuuaguacggaaccguugauucagaucauugguagagcuaucau
cugccggauaacggcugagccagaauccgugccagagcgacgauauguau
aaaaauagaggucucuccuguaucguagaagaugggcgaugguugaugaa
acggaaguguuuuauugacuuguccaugaaauuccagaauccauugcaua
cgacuuuaauguggaacgggguauuguagauuaagccuuuguucccaaga
uuu

........................<<<<<<.>>>>>.>.........<<.
...>>..<<<<<......<<>>.....>>>>>..<<<..<<.......<<
.<<<<<<<.......>>>>>>>.>>.......>>....<<<<<.<<<<<.
.<<<<<..<<<<<<<<<.....<<<<......>>>>.((......<<<..
...))>>>......>>>>>>>>>..>>>>>.....>>>>>>>>.>>....
....<<<<<<..>>>>>>.....<<<<<..>>>>>..>>>..........
........................<<....>>...............<<<
<....>>>>.............................<<<<<<<<<<<<
.....<<<.<<<<.<<<.<<.<<<<<<<..>>>>>>>>>...>>>..>>>
>..>>>..<<<<<<<.<<<<<<<<<<<<<<<.......<<<<<<<<....
..<...>......>>>>>>>>......>>>>>.>>>>>>>>>>.......
.<<<.....>>>.....>>>>>>>....>>>>>>>>>>>>..<<<<..>>
>><<<<<<...<<<<<<<.<<<<<<..<<<...<<<<.......>>>>..
.>>>...<<<<..<<<<<..>>>>>...>>>>....>>>>>>.>>>>>>>
.<<.<...<<<<<<..>>>>>>......>.>>..<<<<<<.....<<<<<
<<<.....<<<.<<...>>>>>..>>>>>>.>>..<<<<....>>>>...
.<<<.>>>...<<<<<<<...<<<(((.....<<<<<<.<<..<<<.<<<
<<<..>>>>>>>>>.))).>>...>>>>>>.>>>...>>>>>>><<<<<<
<<<............>>>>>>>>>....>>>>>>..<<<<<<<<.<<...
...<<<<.<<<<....>>>>>>>>...>>>>>>>>>>........>>>>>
><<.....<<.>>....>>....<<<<<...>>>>><<<<<<<..<<<<.
<.<<<<<......<>.....>>>>>.><<<<<...<<<<...>>>>..>>
>>>.<<<<....<<<<.>>>>....>>>>.>>>>..>>>>>>>.....<<
.......<<<<..>>>>....>>........<<<<<....<<<<<<<<<<
<<......>>>>>>>>>>>>..>>>>>.........<<<<<.<.......
......>.....<<<<<<<<<.<<<...>>>....>>>>.>>>>>...>>
>>>

Receptor-Ligand Complex Structure

Structure summary

• PDB: 8eth Chromatin localization of nucleophosmin organizes ribosome biogenesis.
• Resolution: 3.8 Å
• Binding residue (original residue number in PDB): R69 E70 Y77 L82 R83 Q84 R88 R92 T95 Y97 R101 K102 K105 I107 K108 R111 Y112 F113
• Binding residue (residue number reindexed from 1): R63 E64 Y71 L76 R77 Q78 R82 R86 T89 Y91 R95 K96 K99 I101 K102 R105 Y106 F107

Gene Ontology

Molecular Function

• GO:0003729 mRNA binding
• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0000463 maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
• GO:0002181 cytoplasmic translation
• GO:0006412 translation

Cellular Component

• GO:0005634 nucleus
• GO:0005730 nucleolus
• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:8eth, PDBe:8eth, PDBj:8eth
• PDBsum: 8eth
• PubMed: 36423630
• UniProt: O74904|RL35_SCHPO Large ribosomal subunit protein uL29 (Gene Name=rpl35)