Structure of PDB 7u0h Chain h Binding Site BS01

Receptor Information

>7u0h Chain h (length=119) Species: 1247190 (Saccharomyces cerevisiae BY4741)

AGVKAYELRTKSKEQLASQLVDLKKELAELKVQKLSRPSLPKIKTVRKSI
ACVLTVINEQQREAVRQLYKGKKYQPKDLRAKKTRALRRALTKFEASQVT
EKQRKKQIAFPQRKYAIKA

Ligand information

>7u0h Chain 1 (length=2722)

guuugaccucaaaucagguaggaguacccgcugaacuuaagcauaucaau
aagcggaggaaaagaaaccaaccgggauugccuuaguaacggcgagugaa
gcggcaaaagcucaaauuugaaaucugguaccuucggugcccgaguugua
auuuggagagggcaacuuuggggccguuccuugucuauguuccuuggaac
aggacgucauagagggugagaaucccguguggcgaggagugcgguucuuu
guaaagugccuucgaagagucgaguuguuugggaaugcagcucuaagugg
gugguaaauuccaucuaaagcuaaauauuggcgagagaccgauagcgaac
aaguacagugauggaaagaugaaaagaacuuugaaaagagagugaaaaag
uacgugaaauuguugaaagggaagggcauuugaucagacaugguguuuug
ugcccucugcuccuuguggguaggggaaucucgcauuucacugggccagc
aucaguuuugguggcaggauaaauccauaggaauguagcuugccucggua
aguauuauagccugugggaauacugccagcugggacugaggacugcgacg
uaagucaaggaugcuggcauaaugguuauaugccgcccgucuugaaacac
ggaccaaggagucuaacgucuaugcgaguguuuggguguaaaacccauac
gcguaaugaaagugaacguagguuggggccucgcaagaggugcacaaucg
accgauccugaugucuucggauggauuugaguaagagcauagcuguuggg
acccgaaagauggugaacuaugccugaauagggugaagccagaggaaacu
cugguggaggcucguagcgguucugacgugcaaaucgaucgucgaauuug
gguauaggggcgaaagacuaaucgaaccaucuaguagcugguuccugccg
aaguuucccucaggauagcagaagcucguaucaguuuuaugagguaaagc
gaaaacuuuaaauauguaaaaugaagagcuuuuagugggccauuuuuggu
aagcagaacuggcgaugcgggaugaaccgaacguagaguuaaggugccgg
aauacacgcucaucagacaccacaaaagguguuaguucaucuagacagcc
ggacgguggccauggaagucggaauccgcuaaggaguguguaacaacuca
ccggccgaaugaacuagcccugaaaauggauggcgcucaagcguguuacc
uauacucuaccgucaggguuggaugcccugacgaguaggcaggcguggag
gucagugacgaagccuagaccguaaggucgggucgaacggccucuagugc
agaucuuggugguaguagcaaauauucaaaugagaacuuugaagacugaa
guggggaaagguuccacgucaacagcaguuggacguggguuagucgaucc
uaagagauggggaagcuccguuucaaaggccugauuuuaugcaggccacc
aucgaaagggaauccgguuaagauuccggaaccuggauauggauucuuca
cgguaacguaacugaauguggagacgucggcgcgagcccugggaggaguu
aucuuuucuucuuaacagcuuaucaccccggaauugguuuauccggagau
ggggucuuauggcuggaagaggccagcaccuuugcuggcuccggugcgcu
ugugacggcccgugaaaauccacaggaaggaauaguuuucaugccagguc
guacugauaaccgcagcaggucuccaaggugaacagccucuaguugauag
aauaauguagauaagggaagucggcaaaauagauccguaacuucgggaua
aggauuggcucuaagggucggguagugagggccuuggucagacguagacg
gccuuggugucgcuugcuacaauuaacgaucaacuuagaacugguacgga
caaggggaaucugacugucuaauuaaaacauagcauugcgauggucagaa
agugauguugacgcaaugugauuucgauuaacgagauucccacugucccu
aucuacuaucuagcgaaaccacagccaagggaacgggcuuggcagaauca
gcggggcccuguugagcuuuuagucccucggaauuugaggcuagaaguua
ccacagggauaacuggcuuguggcagucaagcguucauagcgacauugcu
uuuugauugaugucggcucuuccuaucauaccgaagcagaauucgguaag
cguuggauuguucacccacuaauagggaagcuggaccgucgugagacagg
uuaguuuacccuacugaugaauguuaccgcaauaguaauugaacuuagua
cgagaggaacaguucauucggauaauugguuuuugcggcugucugaucag
gcauugccgcgaagcuaccauccgcuggauuauggcugaacgccucuaag
ucagaauccaugcuagaacgcggugauuucuuugcuccacacaauauaga
uggauacgaauaaggcguccuuguggcgucgcugaaccauagcaggcuag
caacggugcacuuggcggaaaggccuugggugcuugcuggcgaauugcaa
ugucauuuugcguggggauaaaucauuuguauacgacuuagauguacaac
gggguauuguaagcaguagaguagccuuguuguuacgaucugcugagauu
aagccuuuguugucugauuugu

..........................<<<<<<.....<<....>>.....
.>>>>>.>.........<<....>>..<<<<<......<<.....>>...
..>>>>>..<<<...........<<.<<<<<<...>>>>>>.>>......
.......<<<<<<.<<<<<<<<<<<<<<<<<<<<<<....<<<<......
>>>>.(.......<<<......)>>>..>.>>>>>>>>>..>>>>>>>..
..>>>>>>>>>>>........<<<<<<<.<....>.>>>>>>>.....<<
<<<..((((((>>>>>..>>>.............................
.....<<....>>...............<<<<....>>>>..........
........................<<<<<.<<<<<......<<<<....<
<..<<<<<<.<........>>>>>>>......>>....>>>>..<<<<<<
<.<<<<<<<<<<<<<<<.....<<<<<<<<<....<.<<<<<......>>
>>>>......>>>>>>>.>.>.>>>>>.>>>>>>>>>>........<<<.
...>>>.....>>>>>>>.....>>>>>.>>>>>..<<<<.<....>.>>
>><<<<<<....<<<<<<.<<<<<<..<<<...<<<<.....>>>>...>
>>...<<....>>.......<<<<<..<<<<<....>>>>>...>>>>>.
<<<<<<<<...<<....>>...>>>>.>>.>>....>>>>>>.>>>>>>.
.<<.<..<<<<<<<.....<<<<<<<<<..<<<....<<<<<<<....>>
>>>>>.....>>>...<<<<.<<.<<........>>>>>>>>....>>>>
>>>>>...<<.......>>......>>>>>>>.....>.>>..<<<<<<.
...<.<<......>>.><<<<<<<<<<...<<<.<<<<<<..........
...............>>>>>>>>>.>>>>>>>>.>>..<<<<.<<<<<..
...>>>>>.>>>>....<<<......>>>...<<<<<<<..<<<<(((..
...<<<<<<.....<<..........<<<.<<<<<<<<<<.......<<<
<<.<<<...<<.........>>...>>>.........<<......>>...
>>>>>...>>>>>>>>>>>>>..........))).>>...>>>>>>.>>>
>..>>>>>>><<<<<<<<<.....>>>>>>>>>....>>>>>><.<<<<<
<<<.<<......<<<<.<<<<....>>>>>>>>...>>>>>>>>>>..>.
....>>>>>><<.....<<.....<<<<<<.......>>>>>><<<<...
..<<<<<.<<...<<<<<<...<<.....>>.>>>>>>....((<<<<<<
...<<....<<<....>>>........<<<<<<........>>>>>>...
.>>....>>...<<<<<........>>>>>))<<<<.<<<<<<<<....<
<<....>>>...<...<<<<<<...<<<<.<<<<<<<<<<<<<<<<<<..
[[[..>>>>>><...<<<<<....<<<<<<....<<<.....>>>....>
>>>>>.....>>>>>....><<<<<<<<....>>>>>>>>>>>>.>.>>>
>>>>.>>>>.........>>>>>>...>.....>>>>..>>>>>>>>>>>
>..>>.....<<......>>>>>>>.<<<<.....>>>>..>>>>..<<<
<.....<<<<<<<.<<...............<<<<<..............
.>>>>><<.<<<<<<<<<<<..<<<<<<<.....................
............>>>>>>>.......>>>>>..>>>>>>.>>........
....<<<<<<<<<..<<<.........>>>..<<<<<<<....<<<<...
..>>>>......>>>>>>>....<..>....>>.>>>>>>>.....>>.>
>>>>>>..>>>>>>....>>....<<<<<........>>>>>....<<<<
<..<<<<<<<<<.<<<<<<<<<...<<<<.))))))>>>>.>>>>>>>>.
>.>>>>>>..............<<<<<...<<.......>>...>>>>>.
........((....<<<..<<<<<..<.<<<<<<<......>>>>>>>..
.>.<<<.....))..>>>.....>>>>>.>>>.<<<<<<<....>>>.>>
>>.......>>>.>>>>>....<<<<<<<<..<<<<.<.<<<<<<.....
..........>>>>>>.><<<<<...<<<<....<<<<<<<<<<]]].>>
>>>..>>>>>...>>>>..>>>>>.<<<<...<<<<<...........>>
>>>...>>>>.>>>>....>>>>>>>>.......<<<<<<.<<<.<<...
.<<..........<<<<<<.....>>>>>>......>>.......<<<<<
<...<<<<<.<..<<<......>>>..>.>>>>>.>>>>>>.........
....>>.>>>.>>>>>>..<<<<<<<.............>.....<<<<<
<<<<.<<<....<<<<.<..<<<<<......>>>>>..>>>>>...>>>.
...>>>>.>>>>>.>>>>>>..

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7u0h Sequence-specific remodeling of a topologically complex RNP substrate by Spb4.
• Resolution: 2.76 Å
• Binding residue (original residue number in PDB): G72 Y75 R81 R86 R90 T93 K94 F95 K103 Q104 K106 K107 I109
• Binding residue (residue number reindexed from 1): G71 Y74 R80 R85 R89 T92 K93 F94 K102 Q103 K105 K106 I108

Gene Ontology

Molecular Function

• GO:0003729 mRNA binding
• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0000463 maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
• GO:0002181 cytoplasmic translation
• GO:0006412 translation

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:0030687 preribosome, large subunit precursor
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:7u0h, PDBe:7u0h, PDBj:7u0h
• PDBsum: 7u0h
• PubMed: 36482249
• UniProt: P0CX84|RL35A_YEAST Large ribosomal subunit protein uL29A (Gene Name=RPL35A)