Structure of PDB 7nhl Chain h Binding Site BS01

Receptor Information

>7nhl Chain h (length=138) Species: 93061 (Staphylococcus aureus subsp. aureus NCTC 8325)

VLPDPIHNSKLVTKLINKIMLDGKRGTAQRILYSAFDLVEQRSGRDALEV
FEEAINNIMPVLEVKARRSNYQVPVEVRPERRTTLGLRWLVNYARLRGEK
TMEDRLANEILDAANNTGGAVKKREDTHKMAEANKAFA

Ligand information

>7nhl Chain a (length=1538)

ggagaguuugauccuggcucaggaugaacgcuggcggcgugccuaauaca
ugcaagucgagcgaacggacgagaagcuugcuucucugauguuagcggcg
gacgggugaguaacacguggauaaccuaccuauaagacugggauaacuuc
gggaaaccguagcuaauaccggauaauauuuugaaccgcaugguucaaaa
gugaaagacggucuugcugucacuuauagauggauccgcgcugcauuagc
uaguugguaagguaacggcuuaccaaggcaacgaugcauagccgaccuga
gagggugaucggccacacuggaacugagacacgguccagacuccuacggg
aggcagcaguagggaaucuuccgcaaugggcgaaagccugacggagcaac
gccgcgugagugaugaaggucuucggaucguaaaacucuguuauuaggga
agaacauauguguaaguaacugugcacaucuugacgguaccuaaucagaa
agccacggcuaacuacgugccagcagccgcgguaauacguagguggcaag
cguuauccggaauuauugggcguaaagcgcgcguaggcgguuuuuuaagu
cugaugugaaagcccacggcucaaccguggagggucauuggaaacuggaa
aacuugagugcagaagaggaaaguggaauuccauguguagcggugaaaug
cgcagagauauggaggaacaccaguggcgaaggcgacuuucuggucugua
acugacgcugaugugcgaaagcguggggaucaaacaggauuagauacccu
gguaguccacgccguaaacgaugagugcuaaguguuaggggguuucccgc
cccuuagugcugcagcuaacgcauuaagcacuccgccuggggaguacgac
cgcaagguugaaacucaaaggaauugacggggacccgcacaagcggugga
gcaugugguuuaauucgaagcaacgcgaagaaccuuaccaaaucuugaca
uccuuugacaacucuagagauagagccuuccggacaaagugacagguggu
gcaugguugucgucagcucgugucgugagauguuggguuaagucccgcaa
cgagcgcaacccuuaagcuuaguugccaucauuaaguugggcacucuaag
uugacugccggugacaaaccggaggaagguggggaugacgucaaaucauc
augccccuuaugauuugggcuacacacgugcuacaauggacaauacaaag
ggcagcgaaaccgcgaggucaagcaaaucccauaaaguuguucucaguuc
ggauuguagucugcaacucgacuacaugaagcuggaaucgcuaguaaucg
uagaucagcaugcuacggugaauacguucccgggucuuguacacaccgcc
cgucacaccacgagaguuuguaacacccgaagccgguggaguaaccuuuu
aggagcuagccgucgaaggugggacaaaugauuggggugaagucguaaca
agguagccguaucggaaggugcggcuggaucaccuccu

...<<<<..[.((((.>>>>.<<<<.<<<<<..<<<<<<<<.....<<<.
<<<..<<<..<<.<<<<...<<<.........>.>>...>>>>.>>>>>.
.....<<........<<<<<<<..<<...<<<<<<<.<<<<.....<<.<
<<....>>>.>>......>>>>......<<<.<<<<<<..>>>>>>>>>.
......<<<<<<...>>>>>>.>>>>>>>..>>>>>>>>>.<<<....<<
<..<<<<<<<<.......>>>>>>>>>>>......>>>..<<<<<<<<..
..>>>>...>>>>.>>.<<<<<.<.........>>>>>>.<<<<<..>>>
>>...>>>>>>.........<<<....<<<<....>>>>..>>>..>>.>
>>>>>..<<<<......<<<<....>>>>.....>>>>...<<<<<<<..
.....<.<<<<<<<........>>>>>>>.>........>>>>>>...>.
.<<<<<(((...<<<<<.....<<.)))>>.......>>>>>>>>>>..>
>>>>>>>>..........<<<((.....<<<<<..<<<.<<<<<<<.<<<
<<<<<<.......<<<<<<.....>>>>>>.....>>>>>>>..>>>>>>
>>>...<<<<<<<<...<<<<<<<....<<<<<<<<...<<<......>>
>......>>>>>>>.>..........<<....>>.>>>>>>>..>>>>>.
>>>...>>>..>>>>>....<<<<<<<...<...<<<<.<.....>.>>>
>...>>>>>>>>..........<<<<<<..<<<<<<<<<<<<<.....>>
>>>>>>>>>>>...<<..))>>.....>>>>>>.>>>.<<<......<<<
<....>>>>....>>>..)))).]<<<<<.<<<<<<<.<<.<<<<<<..<
<<<<<<<<<......<<........>>..........<<<<<<<......
<<<<<<.....<<<<<....>>>>>..........>>>>.>>.<<<.<<<
..<<<<<<.......<<<<<<<<<....>>>..<<<<......>>>>..>
>>>>>.....<<<<.<<<<<<<..<<<..<<......>>>>>...>>>>>
>>.....<<<<<.....>>>>>........>>>>.........>>>...>
>>>>>>>>...>>>>>>>...>>.>>>>>>>>.....<<<<<<<.....<
<<..<<...<<<....>>>...>>....>>>.....>>>>>>>......<
..<.<<<<<<<........>>>>>>>>...>.....>>>>>>....<<<<
<<<.........>>>>>>>......>>...>>>>>>>>>>.>>....<..
<<.<.<<<<.<<<..<<<<<<..<<<<....<.<<<<..<<<..<<<...
>>>.>>>.>>>>.>...>>>>..>>>>>>..>>>.>>>>..>.>>...>.
....<<<<<<<<<....>>>>>>>>>............

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7nhl Structural basis of ABCF-mediated resistance to pleuromutilin, lincosamide, and streptogramin A antibiotics in Gram-positive pathogens.
• Resolution: 3.1 Å
• Binding residue (original residue number in PDB): N28 I30 M31 L32 D33 G34 K35 R36 T38 R41 R92 E94 R95 T98 R102 R109 K114 M116
• Binding residue (residue number reindexed from 1): N17 I19 M20 L21 D22 G23 K24 R25 T27 R30 R78 E80 R81 T84 R88 R95 K100 M102

Gene Ontology

Molecular Function

• GO:0000049 tRNA binding
• GO:0003723 RNA binding
• GO:0003729 mRNA binding
• GO:0003735 structural constituent of ribosome
• GO:0019843 rRNA binding

Biological Process

• GO:0000028 ribosomal small subunit assembly
• GO:0006412 translation

Cellular Component

• GO:0005840 ribosome
• GO:0015935 small ribosomal subunit
• GO:0022627 cytosolic small ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:7nhl, PDBe:7nhl, PDBj:7nhl
• PDBsum: 7nhl
• PubMed: 34117249
• UniProt: P48940|RS7_STAA8 Small ribosomal subunit protein uS7 (Gene Name=rpsG)