Structure of PDB 7bt6 Chain h Binding Site BS01

Receptor Information

>7bt6 Chain h (length=119) Species: 559292 (Saccharomyces cerevisiae S288C)

AGVKAYELRTKSKEQLASQLVDLKKELAELKVQKLSRPSLPKIKTVRKSI
ACVLTVINEQQREAVRQLYKGKKYQPKDLRAKKTRALRRALTKFEASQVT
EKQRKKQIAFPQRKYAIKA

Ligand information

>7bt6 Chain 1 (length=2962)

guuugaccucaaaucagguaggaguacccgcugaacuuaagcauaucaau
aagcggaggaaaagaaaccaaccgggauugccuuaguaacggcgagugaa
gcggcaaaagcucaaauuugaaaucugguaccuucggugcccgaguugua
auuuggagagggcaacuuuggggccguuccuugucuauguuccuuggaac
aggacgucauagagggugagaaucccguguggcgaggagugcgguucuuu
guaaagugccuucgaagagucgaguuguuugggaaugcagcucuaagugg
gugguaaauuccaucuaaagcuaaauauuggcgagagaccgauagcgaac
aaguacagugauggaaagaugaaaagaacuuugaaaagagagugaaaaag
uacgugaaauuguugaaagggaagggcauuugaucagacaggccagcauc
aguuuugguggcaggauaaauccauaggaauguagcuugccucgguaagu
auuauagccugugggaauacugccagcugggacugaggacugcgacguaa
gucaaggaugcuggcauaaugguuauaugccgcccgucuugaaacacgga
ccaaggagucuaacgucuaugcgaguguuuggguguaaaacccauacgcg
uaaugaaagugaacguagguuggggccucgcaagaggugcacaaucgacc
gauccugaugucuucggauggauuugaguaagagcauagcuguugggacc
cgaaagauggugaacuaugccugaauagggugaagccagaggaaacucug
guggaggcucguagcgguucugacgugcaaaucgaucgucgaauuugggu
auaggggcgaaagacuaaucgaaccaucuaguagcugguuccugccgaag
uuucccucaggauagcagaagcucguaucaguuuuaugagguaaagcgaa
aacuuuaaauauguaagaaguccuuguuugaacguggacauuugaaugaa
gagcuuuuagugggccauuuuugguaagcagaacuggcgaugcgggauga
accgaacguagaguuaaggugccggaauacacgcucaucagacaccacaa
aagguguuaguucaucuagacagccggacgguggccauggaagucggaau
ccgcuaaggaguguguaacaacucaccggccgaaugaacuagcccugaaa
auggauggcgcucaagcguguuaccuauacucuaccgucaggguugauau
gaugcccugacgaguaggcaggcguggaggucagugacgaagccuagacc
guaaggucgggucgaacggccucuagugcagaucuuggugguaguagcaa
auauucaaaugagaacuuugaagacugaaguggggaaagguuccacguca
acagcaguuggacguggguuagucgauccuaagagauggggaagcuccgu
uuccaucgaaagggaauccgguuaagauuccggaaccuggauauggauuc
uucacgguaacguaacugaauguggagacgucggcgcgagcccugggagg
aguuaucuuuucuucuuaacagcuuaucaccccggaauugguuuauccgg
agauggggucuuauggcuggaagaggccagcaccuuugcuggcuccggug
cgcuugugacggcccgugaaaauccacaggaaggaauaguuuucaugcca
ggucguacugauaaccgcagcaggucuccaaggugaacagccucuaguug
auagaauaauguagauaagggaagucggcaaaauagauccguaacuucgg
gauaaggauuggcucuaagggucggacgaucaacuuagaacugguacgga
caaggggaaucugacugucuaauuaaaacauagcauugcgauggucagaa
agugauguugacgcaaugugauuucugcccagugcucugaaugucaaagu
gaagaaauucaaccaagcgcggguaaacggcgggaguaacuaugacucuc
uuaagguagccaaauggauuaacgagauucccacugucccuaucuacuau
cuagcgaaaccacagccaagggggcuuggcagaaucagcggggaaagaag
acccuguugagcuugacucuaguuugacauugugaagagacauaguuucu
uuacuuauucaagacauugucagguggggaguuugggggcggcacaucug
uuaaacgauaacgcagauguccuaaggggggcucauggagaacagaaauc
uccaguagaacaaaaggguaaaagcccccuugauuuugauuuucagugug
aauacaaaccaugaaaguguggccuaucgauccuuuagucccucggaauu
ugaggcuagaggugccagaaaaguuaccacagggauaacuggcuuguggc
agucaagcguucauagcgacauugcuuuuugauucuucgaugucggcucu
uccuaucauaccgaagcagaauucgguaagcguuggauuguucacccacu
aauagggagugagcuggguuuagaccgucgugagacagguuaguuuuacc
cuacugaugaauguuaccgcaauaguaauugaacuuaguacgagaggaac
aguucauucggauaauugguuuuugcggcugucugaucaggcauugccgc
gaagcuaccauccgcuggauuauggcugaacgccucuaagucagaaucca
ugcuagaacgcggugauuucuuugcuccacacaauauagauggauacgaa
uaaggcguccuuguggcgucgcugaaccauagcaggcuagcaacggugca
cuuggcggaaaggccuugggugcuugcuggcgaauugcaaugucauuuug
cguggggauaaaucauuuguauacgacuuagauguacaacgggguauugu
aagcaguagaguagccuuguuguuacgaucugcugagauuaagccuuugu
ugucugauuugu

..........................<<<<<<.....<<....>>.....
.>>>>>.>.........<<....>>..<<<<<......<<.....>>...
..>>>>>..[[[..<<.......<<.<<<<<<...>>>>>>.>>......
.>>....<<<<<..<<<<<..<<<<<<<<<<<<<<<....<<<<......
>>>>.(.......<<<......)>>>..>.>>>>>>>>..>>>>>>....
..>>>>>.>>>>>........<<<<<<<.<....>.>>>>>>>.....((
(((..<<<<<<)))))..]]].............................
.....<<....>>...............<<<<....>>>>..........
........................<<<<<.<<<<<<.....<<<<<<<.<
<<<<<<<<<<<<<<.....<<<<<<<<<......<<<<<<....>>>>>>
.......>>>>>>>.>.>.>>>>>.>>>>>>>>>>........<<<....
>>>.....>>>>>>>....>>>>>>.>>>>>..<<<<........>>>><
<<<<<....<<<<<<.<<<<<<..<<<...<<<<.....>>>>...>>>.
..<<....>>.......<<<<<..<<<<<....>>>>>...>>>>>.<<<
<<<<<....<....>....>>>>.>>.>>....>>>>>>.>>>>>>..<<
.<...<<<<<<.....<<<<<<<<<..<<<....<<<<<<<....>>>>>
>>.....>>>...<<<<.<<.<<........>>>>>>>>....>>>>>>>
>>...<<.......>>......>>>>>>......>.>>..<<<<<<....
<.<<<....>>>.><<.<<.<......<<<.<<<<<<<............
...........>>>>>...<<<<..<<....>>..>>>>.....>>>>>.
....>.>>.>>..<<<<.<<<<<.....>>>>>.>>>>....<<<.....
.>>>...<<<<<<<..<<<<(((.....<<<<<<.....<<.........
.<<<.<<<<<<<<<<.......<<<<<.<<....<<.........>>...
.>>.........<<......>>...>>>>>...>>>>>>>>>>>>>....
......))).>>...>>>>>>.>>>>..>>>>>>><<<<<<<<<......
...>>>>>>>>>....>>>>>><.<<<<<<<..<<......<<<<.<<<<
....>>>>>>>>...>>.>>>>>>>..>.....>>>>>><<<....<<..
...<<<<<<.......>>>>>><<<<.....<<<<<.<<...<<<<<<..
.<<.....>>.>>>>>>....((<<<<<<...<<....<<<....>>>..
.....>>....>>...<<<<<........>>>>>))<<<<.<<<<<<<..
...<<<....>>>.......<<<<<<...<<<<.<<<<<<<.<<<<<<<<
<<..{{{..>>>>>><...<<<<<....<<<<<<....<<<.....>>>.
...>>>>>>.....>>>>>....><<<<<<<......>>>>>>>>>>>..
.>>>>>>>.>>>>.........>>>>>>..........>>>..>>>>>>>
>>>>>..>>.....<<......>>>>>>>.<<<<.....>>>>..>>>>.
.<<<<.....<<<<<<<.<<.........<.....<<<<<....<<..>>
.....>>>>><<.<<<<<<<<<<<..>>>>>..>>>>>>.>>........
....<<<<<<<<<..<<<.........>>>..<<<<<<<....<<<<...
..>>>>......>>>>>>>...............<<.............<
<<<....>>>>..........>>...........<<.........>>...
..........<<..>>......>>.>>>>>>>..>..>>.>>>>>>>..>
>>>>>...>>>....<<<<<....>>>>>....[[[[[..{{{.......
.((((((..[[[[..<<<<<..<<.<.<<..<<<<<.............>
>>>>...<...>.....>>.>.>>...>>>>>......<.<..<<<<<<<
<...........>>>>>>>>.....<<<<<<<...<<<<<<.......>>
>>>>.....<<......>>....>>>>>>>...<<<.<<<<<.......>
>>>.>>>>.<<<....>>>...>.>...[...(((((...((((.>>>>>
>)))).)))))....].....]]]]...))))))..............<<
<<<...<<.......>>...>>>>>.................<<<<<...
<<<<..<.<<<<<<<......>>>>>>>...>.<<<.........>>>..
...>>>>.....>>>>>.....<<<<<<<....>>>.>>>>........}
}}.]]]]]....<<<<<<<<..<<<<.<.<<<<<<...............
>>>>>>.><<<<<...<<<<....<<<<<<<<<.}}}..>>>>..>>>>>
...>>>>..>>>>>.<<<<...<<<<<...........>>>>>...>>>>
.>>>>....>>>>>>>>.......<<<<<<.<<<.<.....<<.......
...<<<<<<.....>>>>>>......>>.......<<<<<<...<<<<<.
<..<<<......>>>..>.>>>>>.>>>>>>..............>.>>>
.>>>>>>..<<<<<<<.............>.....<<<<<<<<<.<<<..
..<<<<.<..<<<<<......>>>>>..>>>>>...>>>....>>>>.>>
>>>.>>>>>>..

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7bt6 Structural insights into assembly of the ribosomal nascent polypeptide exit tunnel.
• Resolution: 3.12 Å
• Binding residue (original residue number in PDB): Y75 R90 F95 K103 K106
• Binding residue (residue number reindexed from 1): Y74 R89 F94 K102 K105

Gene Ontology

Molecular Function

• GO:0003729 mRNA binding
• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0000463 maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
• GO:0002181 cytoplasmic translation
• GO:0006412 translation

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:0030687 preribosome, large subunit precursor
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:7bt6, PDBe:7bt6, PDBj:7bt6
• PDBsum: 7bt6
• PubMed: 33037216
• UniProt: P0CX84|RL35A_YEAST Large ribosomal subunit protein uL29A (Gene Name=RPL35A)