Structure of PDB 7nwg Chain g3 Binding Site BS01

Receptor Information

>7nwg Chain g3 (length=114) Species: 9986 (Oryctolagus cuniculus)

VQRLTYRRRLSYNTASNKTRLSRTPGNRIVYLYTKKVGKAPKSACGVCPG
RLRGVRAVRPKVLMRLSKTKKHVSRAYGGSMCAKCVRDRIKRAFLIEEQK
IVVKVLKAQAQSQK

Ligand information

>7nwg Chain 51 (length=3626)

cgcgaccucagaucagacguggcgacccgcugaauuuaagcauauuaguc
agcggaggaaaagaaacuaaccaggauucccucaguaacggcgagugaac
agggaagagcccagcgccgaauccccgccccgcggcggggcgcgggaaau
guggcguacggaagacccacuccccggcgccggggggcccaaguccuucu
gaucgaggcccagcccguggacggugugaggccgguagcggcccccggcg
cgccgggcccgggucuucccggagucggguugcuugggaaugcagcccaa
agcgggugguaaacuccaucuaaggcuaaauaccggcacgagaccgauag
ucaacaaguaccguaagggaaaguugaaaagaacuuugaagagagaguuc
aagagggcgugaaaccguuaagagguaaacgggugggguccgcgcagucc
gcccggaggauucaacccggcggcgcgguccggccgugccggcggggcgg
aucucccgcucccgucggcgggggaccgccccccggccggcgaccggccg
ccgccgggcgcauuuccaccgcggcggugcgccgcgaccggcuccgggac
ggcugggaaggccccggcggggaagguggcccgggggccccggguguuac
agccccgccccggcagcagcagcucgccgaaucccggggccgagggagcg
agacccgugcgccgcgcucuccccccucgggggggccgggccaccccucc
cacggcgcgaccgcucucccacccggggggcggggcggacuguccccagu
gcgccccgggcgggucgcgccgucgggcccgggggagguucucucggggc
cacgcgagcgcacggggucggcggcgaugucggcuacccacccgacccgu
cuugaaacacggaccaaggagucuaacacgugcgcgagucgggggcuacg
aaagccgccguggcgcaaugaaggugaaggccggcggcgcgccgccggcc
gaggugggaucccgaggccuuccaguccgccgagggcgcaccaccggccc
gucucgcccgccgcgccggggagguggagcacgagcgcacguguuaggac
ccgaaagauggugaacuaugccugggcagggcgaagccagaggaaacucu
gguggagguccguagcgguccugacgugcgaaucggucguccgaccuggg
uauaggggcgaaagacuaaucgaaccaucuaguagcugguucccuccaaa
guuucccucaggauagcuggcgcucuccgcaguuuuauccgguaaagcga
augauuagaggucuuggggccgaaacgaucucaaccuauucucaaacuuu
aaauggguaagaagcccggcucgcugguguggagccgggcguggaaugcg
agugccuagugggccacuuuugguaagcagaacuggcgcugcgggaugaa
ccgaacgccggguuaaggcgcccgaugccgacgcucaucagaccccagaa
aagguguugguugauauagacagcaggaugguggccauggaagucggaau
ccgcuaaggaguguguaacaacucaccugccgaaucaacuagcccugaaa
auggauggcgcuggagcgucgggcccauacccggccgucgcuggcagucg
gcagagcgagaaagagaguggcgcggacgcuacgccgcgacgaguaggag
ggccgcugcggugagccuugaagccuagggcgcgggcccggguggagccg
ccgcaggugcagaucuuggugguaguagcaaauauucaaacgagaacuuu
gaaggccgaaguggagaaggguuccaugugaacagcaguugaacaugggu
cagucgguccugagagaugggcgagcgccguuccgaagggacgggcgaug
gccuccguugcccucagccgaucgaaagggagucggguucagauccccga
auccggaguggcggagaugggcgccgcgaggcguccagugcgguaacgcg
accgaucccggagaagccggcgggagccccggggagaguucucuuuucuu
ugugaagggcagggcgcccuggaauggguucgccccgagagaggggcccg
ugccuuggaaagcgucgcgguuccggcggcguccggugagcucucgcugg
cccuugaaaauccgggggagaggguguaaaucucgcgccgggccguaccc
auauccgcagcaggucuccaaggugaacagccucuggcauguuggaacaa
uguagguaagggaagucggcaagccggauccguaacuucgggauaaggau
uggcucuaagggcuggcagccgacuuagaacuggugcggaccaggggaau
ccgacuguuuaauuaaaacaaagcaucgcgaaggcccgcggcggguguug
acgcgaugugauuucugcccagugcucugaaugucaaagugaagaaauuc
aaugaagcgcggguaaacggcgggaguaacuaugacucucuuaagguagc
caaaugccucgucaucuaauuagugacgcgcaugaauggaugaacgagau
ucccacugucccuaccuacuauccagcgaaaccacagccaagggaacggg
cuuggcggaaucagcggggaaagaagacccuguugagcuugacucuaguc
uggcacggugaagagacaugagagguguagaauaagugggaggcccgggc
cgccggugaaauaccacuacucugaucguuuuuucacugacccggugagg
cgggggggcgagccgggcucucgcuucuggcgccaagcgcccggccggcc
gggcgcgacccgcuccggggacagugccagguggggaguuugacuggggc
gguacaccugucaaacgguaacgcagguguccuaaggcgagcucagggag
gacagaaaccucccguggagcagaagggcaaaagcucgcuugaucuugau
uuucaguacgaauacagaccgugaaagcggggccucacgauccuucugac
cuuuuggguuuuaagcaggaggugucagaaaaguuaccacagggauaacu
ggcuuguggcggccaagcguucauagcgacgucgcuuuuugauccuucga
ugucggcucuuccuaucauugugaagcagaauucaccaagcguuggauug
uucacccacuaauagggaacgugagcuggguuuagaccgucgugagacag
guuaguuuuacccuacugaugauguguuguugccaugguaauccugcuca
guacgagaggaaccgcagguucagacauuugguguaugugcuuggcugag
gagccaauggggcgaagcuaccaucugugggauuaugacugaacgccucu
aagucagaaucccgcccaggcggaacgauagcgccggagccucgguuggc
cucggauagccggucccccgcccgggguccggugcggagagcccuucguc
ccgggaaacggggcggccggaaagggggccgcccucucgcccgucacgca
ccgcacguucguggggaaccuggcgcuaaaccauucguagacgaccugcu
ucugggucgggguuucguacguagcagagcagcugcugcgaucuauugaa
agucagcccucgacacaaggguuugu

.........................<<<<<<.....<......>......
>>>>>.>.........<<....>>..<<<<<<.....<<.....>>....
>>>>>>..[[[..<.<<<...<<<.<<<<<<<...>>>>>>>..>>>...
..>>>.>..<<<<<<<<<....<<<..<<<..<<<<<<<<....<<<.<.
....>>>>.....((......<<<.....))>>>...>.>>>>>>>...>
>>..>>>...>>>>>>>>>.......<<<<<<<........>>>>>>>..
...(((((..<<<<<<)))))..]]]........................
..........<<....>>...............<<<......>>>.....
.............................<<<<<<<<..<<<.<....<<
<<<<<<.........<<<<<<<<<<....<<<<<<<.<<<...<<<<<<<
.<<<<<<<<<......>>>>>>>>>>>>>>>>..>>>>>>>...>>>.>>
>>>>>>>>..........>>>.>>>>>...<<<<<<<.<<<<<<<<<<.<
<<<.........<..<<<<<<<......<<<<<<<<.>>>>>>>>.....
..>>>>>>>...>............>>>>...>>>>>>>>>>.....<..
.......>.........<...<<<<<<..>>>>>>.....<<..<<<.<<
<<<<<<<<<<<<<<....<..<<..>>.>.<<<<<<<.<<........>>
.>>>>>>>.>>>.>>>>>>>>>>.>>>...>>>.............>>..
.......>.........>>.>>>>>....>>>>...>>>>>>>>...<<<
........>>>.<<<<<<....<<<.<<<<<<<<<..<<<...<<<<...
..>>>>.....>>>...<<....>>...<<<<<.<<<<..>>>>.>>>>>
..<<<<<...<<<..<<........[...>>..>>>....>>>>>.<<<<
<<<<<.<<<<......>>>>>>>>>>>.>>....>>>>>>>>>.>>>..<
<.<..<<<<<<<.....<<<<<<<<<..<<<....<.<<<<......>>>
>.>.....>>>...<<<..<..<<........>>.>.>>>....>>>>>>
>>>...<<.......>>......>>>>>>...>..>.>>..<<<<<<...
.<.<<<....>>>.><<<<<<<<<<...<<<.<<<<<<<<....<<<...
.<<<....<<<..<<<<.............>>>>>>>....>>>...>>>
....>>>>>>...<<<<<<<<<.<......>>>>>>>>>>....>>>>>>
>>>>>>.>>>..<<<<.<<<<<.....>>>>>.>>>>....<<<......
>>>...<<<<<<<...<<<(((.......<<<<<.....<<.........
.<<<.<<<..<<<.........<<<.........<<.........>>...
............<<......>>.....>>>.....>>>..>>>>>>....
......))).>>...>>>>>..>>>...>>>>>>><<<<<<.<<<.....
................]................>>>>>>>>>....>>>>
>>.<<<<<<<<<<.<<......<<<<.<<<<....>>>>>>>>...>>>>
>>>>>>.>>.....>>>>>><<<....<<.....<<<<<<.......>>>
>>>.<<<......<<<<.<<...<<<<<<...<<.....>>.>>>>>>..
..((<<<<<<...<<....<<<....>>>.............<<<<<<<<
<...>>>>>>>>>........>>....>>>..<<<<<........>>>>>
))<<<<....<<<......<<<<<......>>>>>....<<<....>>>.
......<<<<<<...<<<<<<<<<<<<<<<<<<<<<<<..{{{..>>>>>
>.....<<<<.<<<<.<<<<.....<<<.....>>>.....>>>>>>>>.
.>>>>......<<<<<<<.......>>>>>>>>>>>.>.>>>>>>>>>>>
>.........>>>>>>.................>>>.>>>>.>>>..>>.
....<<......>>>>>>..<<<<.....>>>>..>>>....<<<<....
.<<<<<<<.<<...............<<<<<...............>>>>
><<.<<<<<<<<<<<.>>>>>..>>>>>>.>>............<<<<<<
<<<..<<...........>>..<<<<<<<....<<<<.....>>>>....
..>>>>>>>....<..<<<..<<<<<<....<<<.....<<<<....>>>
>.....>>>.>>>>..>>>>>...<<.........>>...........<.
..........<<<<<.......>>>>>.....>.....>.....>>.>>>
>>>>.....>>.>>>>>>>..>>>>>>...>>>....<<<<.........
.>>>>.....[[[[..{{{.{{......[[[[....[[[..<<<<..<<<
<<<<<<.<<<<<<....<<.<<<<..........<<<.<...<<<..>>>
.............>.>>>.>>>>.>>.....>>>>>>...<<<<<.....
<<<<.....<........>..................<<<<<<<<..>>>
>>>>>...>>>>..>>>>>....>>>>>>>>>...>>>>...<<<.....
>>>..<<<<<<...........>>>>>>..<<...<<<<<<<...<<<<<
<.......>>>>>>.....<<.[.(((>>....>>>>>>>...<<<.<<<
<<.......>>>>.>>>>.<<<....>>>.>>)))]......((((((..
(((..>>>>>>.)))))))))..........]]].....]]]].......
<<.<.<.<<<<<<...<.........>...>>>>>>...>>.>.>.....
..<<<<<.<<<<<...<...<<<<<......>>>>>.....>.<<<....
.....>>>......>>>>..>...>>>.>>....<<<.<<<....>>>..
>>>....}}..}}}.]]]]......<<<<<<....<<<..<.<<<<<...
..............>>>>>.><<<<.....<<<....<<..<.<<<.}}}
..>>>....>.>>...>>>....>>>><<<<....<<<<...........
..>>>>....>>>>.>>>.....>>>>>>.<<<<<<<..<<<.....<<<
........<<<<<<..<<<<..>>>>>.>>>>>.......>>>...<<<.
.<<<<....<<<.<<<<<........>>>>>..>>>...>>>>..>>>..
.............>>>..>>.>>>>>.<<<<<.<.............>..
..<<.<<<<<<<.<<.....<<<<.<..<<<<<.>>>>>..>>>>>....
>>....>>>>.>>>.>>..>>>>>..

Receptor-Ligand Complex Structure

Structure summary

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• PDB: 7nwg Blasticidin S inhibits mammalian translation and enhances production of protein encoded by nonsense mRNA.
• Resolution: 3.8 Å
• Binding residue (original residue number in PDB): R4 L5 T6 Y7 R10 L11 S12 Y13 S17 K19 R21 R24 P26 R29 K37 K40 R54 R60 P61 K62 L64 M65 R66 L67 S68 K69 T70 K72 Y78 G79 R90 R93 I97 Q100
• Binding residue (residue number reindexed from 1): R3 L4 T5 Y6 R9 L10 S11 Y12 S16 K18 R20 R23 P25 R28 K36 K39 R53 R59 P60 K61 L63 M64 R65 L66 S67 K68 T69 K71 Y77 G78 R89 R92 I96 Q99

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0006412 translation

Cellular Component

• GO:0005737 cytoplasm
• GO:0005783 endoplasmic reticulum
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:7nwg, PDBe:7nwg, PDBj:7nwg
• PDBsum: 7nwg
• PubMed: 34157102
• UniProt: G1TXG5|RL34_RABIT Large ribosomal subunit protein eL34 (Gene Name=RPL34)

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