Structure of PDB 8inf Chain g Binding Site BS01

Receptor Information

>8inf Chain g (length=143) Species: 9606 (Homo sapiens)

KAEAKAKALKAKKAVLKGVHSHKKKKIRTSPTFRRPKTLRLRRQPKYPRK
SAPRRNKLDHYAIIKFPLTTESAMKKIEDNNTLVFIVDVKANKHQIKQAV
KKLYDIDVAKVNTLIRPDGEKKAYVRLAPDYDALDVANKIGII

Ligand information

>8inf Chain 2 (length=3654)

cgcgaccucagaucagacguggcgacccgcugaauuuaagcauauuaguc
agcggaggagaagaaacuaaccaggauucccucaguaacggcgagugaac
agggaagagcccagcgccgaauccccgccccgcggcggggcgcgggacau
guggcguacggaagacccgcuccccggcgccgcucguggggggcccaagu
ccuucugaucgaggcccagcccguggacggugugaggccgguagcggccc
ccggcgcgccgggcccgggucuucccggagucggguugcuugggaaugca
gcccaaagcgggugguaaacuccaucuaaggcuaaauaccggcacgagac
cgauagucaacaaguaccguaagggaaaguugaaaagaacuuugaagaga
gaguucaagagggcgugaaaccguuaagagguaaacgggugggguccgcg
caguccgcccggaggauucaacccggcggcggguccggccgugucggcgg
cccggcggaucuuucccgcgcgggggaccgucccccgaccggcgaccggc
cgccgccgggcgcauuuccaccgcggcggugcgccgcgaccggcuccggg
acggcugggaaggcccggcggggaagguggcucgggggcccccgaguguu
acagcccccccggcagcagcacucgccgaaucccggggccgagggagcga
gacccgucgccgcgcucuccccccucccggcgcgccggggggggccgggc
caccccucccacggcgcgaccgcucggggcggacuguccccagugcgccc
cgggcgggucgcgccgucgggcccgggggaggccacgcgcgcgucccccg
aagagggggacggcggagcgagcgcacggggucggcggcgacgucggcua
cccacccgacccgucuugaaacacggaccaaggagucuaacacgugcgcg
agucgggggcucgcacgaaagccgccguggcgcaaugaaggugaaggccg
gcgcgcucgccggccgaggugggaucccgaggccucuccaguccgccgag
ggcgcaccaccggcccgucucgcccgccgcgccggggagguggagcacga
gcgcacguguuaggacccgaaagauggugaacuaugccugggcagggcga
agccagaggaaacucugguggagguccguagcgguccugacgugcaaauc
ggucguccgaccuggguauaggggcgaaagacuaaucgaaccaucuagua
gcugguucccuccgaaguuucccucaggauagcuggcgcucucgcagacc
cgacgcccgccacgcaguuuuauccgguaaagccuuuaaauggguaagaa
gcccggcucgcuggcguggagccgggcguggaaugcgagugccuaguggg
ccacuuuugguaagcagaacuggcgcugcgggaugaaccgaacgccgggu
uaaggcgcccgaugccgacgcucaucagaccccagaaaagguguugguug
auauagacagcaggacgguggccauggaagucggaauccgcuaaggagug
uguaacaacucaccugccgaaucaacuagcccugaaaauggauggcgcug
gagcgucgggcccauacccggccgucgccggcagucgagaguggacggga
gcggcggcccggagccccgcggacgcuacgccgcgacgaguaggagggcc
gcugcggugagccuugaagccuagggcgcgggcccggguggagccgccgc
aggugcagaucuuggugguaguagcaaauauucaaacgagaacuuugaag
gccgaaguggagaaggguuccaugugaacagcaguugaacaugggucagu
cgguccugagagaugggcgagcgccguuccgaagggacgggcgauggccu
gcccuccgaucgaaagggagucggguucagauccccgaauccggaguggc
ggagaugggcgccgcgaggcguccagugcgguaacgcgaccgaucccgga
gaagccggcgggagccccggggagaguucucuuuucuuugugaagggcag
ggcgcccuggaauggguucgccccgagagaggggcccgugccuuggaaag
cgucgcgguuccggcggcguccggugagcucucgcuggcccuugaaaauc
cgggggagaggguguaaaucucgcgccgggccguacccauauccgcagca
ggucuccaaggugaacagccucuggcauguuggaacaauguagguaaggg
aagucggcaagccggauccguaacuucgggauaaggauuggcucuaaggg
cugggucggucgcggccggcgccuagcagccgacuuagaacuggugcgga
ccaggggaauccgacuguuuaauuaaaacaaagcaucgcgaaggcccgcg
gcggguguugacgcgaugugauuucugcccagugcucugaaugucaaagu
gaagaaauucaaugaagcgcggguaaacggcgggaguacucucuuaagaa
cgagauucccacugucccuaccuacuauccagcgaaaccacagccaaggg
aacgggcuuggcggaaucagcggggaaagaagacccuguugagcuugacu
cuagucuggcacggugaagagacaugagagguguagaauaagugggaggc
ccccggcgcccccccgguguccccgcgaggggcccggggcgggguccgcc
ggcccugcgggccgccggugaaauaccacuacucugaucguuuuuucacu
gacccggugaggcgggggggcgagccccgaggggcucucgcuucuggcgc
caagcgcccggccgcgcgccggccgggcgcgacccgcuccggggacagug
ccagguggggaguuugacuggggcgguacaccugucaaacgguaacgcag
guguccuaaggcgagcucagggaggacagaaaccucccguggagcagaag
ggcaaaagcucgcuugaucuugauuuucaguacgaauacagaccgugaaa
gcggggccucacgauccuucugaccuuuuggguuuuaagcaggagguguc
agaaaaguuaccacagggauauuguggcggccaagcguucauagcgacgu
cgcuuuuugcuucgaugucggcucuuccuaucauugugaagcagaauuca
ccaagcguuggauuguucacccacuaauagggaacgugagcuggguuuag
accgucgugagacagguuaguuuuacccuacugaugauguguuguugcca
ugguaauccugcucaguacgagaggaaccgcagguucagacauuuggugu
augugcuuggcugaggagccaauggggcgaagcuaccaucugugggauua
ugacugaacgccucuaagucagaaucccgcccaggcggaacgauacggca
gcgccgcggagccucgguuggccucggauagccggucccccgccgggguc
cggugcggagugcccuucguccugggaaacggggcgcggccggagaggcg
gccgcccccucgcccgucacgcaccgcacguucguggggaaccuggcgcu
aaaccauucguagacgaccugcuucugggucgggguuucguacguagcag
agcagcucccucgcugcgaucuauugaaagucagcccucgacacaagggu
uugu

.........................<<<<<<.....<<....>>......
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..>>>>>..<<<<<<<<<<...<<.<<<<<........<<<<<<<<....
<<<<......>>>>.....((......<<<.....))>>>...>.>>>>>
>>...>>>>>.>>..>>>>>>>>>>.......<<<<<<<........>>>
>>>>.....<<<<<..((((((>>>>>..>>>..................
................<<....>>...............<<<......>>
>..................................<<<<<<<<..<<<.<
<...<<<<<<<<.<<<.....<<<<<<<<<<<..<<<<<<<.......<<
....<<<<.<<<..<<.<..>.>>>>>>>>>>>......>>>>...>>>>
>>>>>>>>>>......>>>.>>>.>>>>>...<<<<<<<.<<<<<<<<<<
<<<<<...<<..<<<....<<<<......<<<<<<<<..>>>>>>>>...
....>>>>...>>>........>>>>>>..>>>>>>>>>>>.........
...........<<<<<..<<<<<<<............>>>>>>>.<<<<<
<.<<<<.<<<<<<<<<<.<<<<<..<<<<<.<..<......>.>..>>>>
>..>>>>.>.>>>>>>>.>>>...>>>>..>>>>.>>..<<<<<<<<<..
.....>>>>>>>.>>.....>>>>>......>>.>>>>>...>>>>>...
>>>>>>>>..<<<<........>>>>.<<<<<....<<<<<<<<<<<<<.
.<<<...<<<<........>>>>.....>>>...<<....>>...<<<<<
<<......>>>>>>>..<<<<<...<<<..<<<..[[....(((>>>..>
>>....>>>>>.<<<<<<<<<.<<<<......>>>>>>>>>>>.>>....
>>>>>>>>>>>>>..<<.<..<<<<<<<.....<<<<<<<<<..<<<...
.<<<<<<<....>>>>>>>.....>>>...<<<..<<.<<........>>
>>.>>>....>>>>>>>>>...<<.......>>......>>>>>>...>.
.>.>>..<<<<<<....<.<<<....>>>.><<<<<<<<<<.........
.............<<<.<<<<<<<<....<<<..>>>....>>>>>>...
<<<<<<<<<.<......>>>>>>>>>>....>>>>>>>>>>>>.>>>..<
<<<.<<<<<.....>>>>>.>>>>....<<<......>>>...<<<<<<<
...<<<(((.......<<<<<.....<<..........<<<.<<<<<<<<
<........<<<<<.<<....<<.........>>....>>.........<
<......>>...>>>>>....>>>>>>>>>>>>..{.......))).>>.
..>>>>>..>>>...>>>>>>><<<<<<.<<<..........)))....]
]............................>>>>>>>>>....>>>>>>.<
<<<<<<<<<.<<......<<<<.<<<<....>>>>>>>>...>>>>>>>>
>>.>>.....>>>>>.<<<....<<.....<<<<<<.......>>>>>>.
<<<.....<<<<<.<<...<<<<<....<<.....>>..>>>>>....((
<<<<<<...<<....<<<....>>>.............<<<<........
>>>>.....>>....>>>..<<<<<........>>>>>))<<<<....<<
<<<...<<<<<<<....>>>>>>>...<<<....>>>...<..<<<<<<<
...<<<<<<<<<<<<<<<<<<<<<<<..[[[..>>>>>>....<<<<<.<
<<<.<<<<.....<<<.....>>>.....>>>>>>>>..>>>>>.....<
<<<<<<.......>>>>>>>>>>>.>.>>>>>>>>>>>>.........>>
>>>>>..>..........>>.>>>.>>>>.>>>..>>.....<<......
>>>>>>>.<<<<.....>>>>..>>>....<<<<.....<<<<<<<.<<.
..............<<<<<....<<..>>.....>>>>><<.<<<<<<<<
<<<.......................>>>>>..>>>>>>.>>........
....<<<<<<<<<..<<<.........>>>..<<<<<<<....<<<<...
..>>>>......>>>>>>>.......<<<..<<<<<<....<<<....<<
<<<....>>>>.>...>>>.>>>>..>>>>><<<.<...>.>>>......
>>.>>>>>>>.....>>.>>>>>>>..>>>>>>...>>>....<<<<<..
......>>>>>....<<<<<..<<<........<<<<<.<<<<<<..<<<
<<.<<<<<<<<<.<<<<<<<<.<<<.........................
..................................................
....................................>>.>.>>>>>>>>.
..<<<<<.....<<<<........<<....>>..................
...<<<<<<<<<<.......>>>>>>>>>>..>>>>..>>>>>....>>>
>>>>>>..>>>>>...<<<.....>>>.<<<<<<<...........>>>>
>>>.<<...<<<<<<<...<<<<<<.......>>>>>>.....<<.[.((
(>>....>>>>>>>...<<<.<<<<<.......>>>>.>>>>.<<<....
>>>.>>)))].....<<<<<<<..<<<..)))))).>>>>>>>>>>....
.....>>>>..>>>>>>>....}..<<<<<<...<<.......>>...>>
>>>>.........((...<<<<.<<<<<<..<.<.<<<<<......>>>>
>...>.>.<<<.....))..>>>.....>>>>>..>...>>>>......<
<<<<<<....>>>.>>>>........>>>.>>>>>.....<<<<<<.<..
<<<..<.<<<<<.................>>>>>.><<<<....<<<<..
..<<.<<<<<<.]]]..>>>>..>>.>>...>>>>...>>>><<<<<...
<<<<<...........>>>>>...>>>>>.>>>...>.>>>>>>.....<
<<<<<..<<..<<<<....<<<........<<<<<.<<<<....>>>>.>
>>>>.......>>>...<<<..<<<<....<<<<.<<<<<<<......>>
>>>>>>>>>...>>>>..>>>..............>>>>..>>.>>>>>>
<<<<<.<.............>....<<.<<<<<<<.<<.....<<<<.<.
.<<<<<......>>>>>..>>>>>....>>....>>>>.>>>.>>..>>>
>>..

Receptor-Ligand Complex Structure

Structure summary

• PDB: 8inf Visualizing the nucleoplasmic maturation of human pre-60S ribosomal particles.
• Resolution: 3.0 Å
• Binding residue (original residue number in PDB): H33 K37 R47 R48 P49 K50 T51 R53 R55 R56 Q57 K59 Y60 K89 N105 K106 H107 N125 T126 L127 P130 K134 K135 Y137 R139
• Binding residue (residue number reindexed from 1): H20 K24 R34 R35 P36 K37 T38 R40 R42 R43 Q44 K46 Y47 K76 N92 K93 H94 N112 T113 L114 P117 K121 K122 Y124 R126

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003735 structural constituent of ribosome
• GO:0005515 protein binding
• GO:0019843 rRNA binding
• GO:0045296 cadherin binding
• GO:1904841 TORC2 complex binding

Biological Process

• GO:0000027 ribosomal large subunit assembly
• GO:0002181 cytoplasmic translation
• GO:0006412 translation

Cellular Component

• GO:0005634 nucleus
• GO:0005730 nucleolus
• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:0022626 cytosolic ribosome
• GO:0070062 extracellular exosome
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:8inf, PDBe:8inf, PDBj:8inf
• PDBsum: 8inf
• PubMed: 37491604
• UniProt: P62750|RL23A_HUMAN Large ribosomal subunit protein uL23 (Gene Name=RPL23A)