Structure of PDB 7u0h Chain g Binding Site BS01

Receptor Information

>7u0h Chain g (length=112) Species: 1247190 (Saccharomyces cerevisiae BY4741)

AQRVTFRRRNPYNTRSNKIKVVKTPGGILRAQHVKKLATRPKCGDCGSAL
QGISTLRPRQYATVSKTHKTVSRAYGGSRCANCVKERIIRAFLIEEQKIV
KKVVKEQTEAAK

Ligand information

>7u0h Chain 1 (length=2722)

guuugaccucaaaucagguaggaguacccgcugaacuuaagcauaucaau
aagcggaggaaaagaaaccaaccgggauugccuuaguaacggcgagugaa
gcggcaaaagcucaaauuugaaaucugguaccuucggugcccgaguugua
auuuggagagggcaacuuuggggccguuccuugucuauguuccuuggaac
aggacgucauagagggugagaaucccguguggcgaggagugcgguucuuu
guaaagugccuucgaagagucgaguuguuugggaaugcagcucuaagugg
gugguaaauuccaucuaaagcuaaauauuggcgagagaccgauagcgaac
aaguacagugauggaaagaugaaaagaacuuugaaaagagagugaaaaag
uacgugaaauuguugaaagggaagggcauuugaucagacaugguguuuug
ugcccucugcuccuuguggguaggggaaucucgcauuucacugggccagc
aucaguuuugguggcaggauaaauccauaggaauguagcuugccucggua
aguauuauagccugugggaauacugccagcugggacugaggacugcgacg
uaagucaaggaugcuggcauaaugguuauaugccgcccgucuugaaacac
ggaccaaggagucuaacgucuaugcgaguguuuggguguaaaacccauac
gcguaaugaaagugaacguagguuggggccucgcaagaggugcacaaucg
accgauccugaugucuucggauggauuugaguaagagcauagcuguuggg
acccgaaagauggugaacuaugccugaauagggugaagccagaggaaacu
cugguggaggcucguagcgguucugacgugcaaaucgaucgucgaauuug
gguauaggggcgaaagacuaaucgaaccaucuaguagcugguuccugccg
aaguuucccucaggauagcagaagcucguaucaguuuuaugagguaaagc
gaaaacuuuaaauauguaaaaugaagagcuuuuagugggccauuuuuggu
aagcagaacuggcgaugcgggaugaaccgaacguagaguuaaggugccgg
aauacacgcucaucagacaccacaaaagguguuaguucaucuagacagcc
ggacgguggccauggaagucggaauccgcuaaggaguguguaacaacuca
ccggccgaaugaacuagcccugaaaauggauggcgcucaagcguguuacc
uauacucuaccgucaggguuggaugcccugacgaguaggcaggcguggag
gucagugacgaagccuagaccguaaggucgggucgaacggccucuagugc
agaucuuggugguaguagcaaauauucaaaugagaacuuugaagacugaa
guggggaaagguuccacgucaacagcaguuggacguggguuagucgaucc
uaagagauggggaagcuccguuucaaaggccugauuuuaugcaggccacc
aucgaaagggaauccgguuaagauuccggaaccuggauauggauucuuca
cgguaacguaacugaauguggagacgucggcgcgagcccugggaggaguu
aucuuuucuucuuaacagcuuaucaccccggaauugguuuauccggagau
ggggucuuauggcuggaagaggccagcaccuuugcuggcuccggugcgcu
ugugacggcccgugaaaauccacaggaaggaauaguuuucaugccagguc
guacugauaaccgcagcaggucuccaaggugaacagccucuaguugauag
aauaauguagauaagggaagucggcaaaauagauccguaacuucgggaua
aggauuggcucuaagggucggguagugagggccuuggucagacguagacg
gccuuggugucgcuugcuacaauuaacgaucaacuuagaacugguacgga
caaggggaaucugacugucuaauuaaaacauagcauugcgauggucagaa
agugauguugacgcaaugugauuucgauuaacgagauucccacugucccu
aucuacuaucuagcgaaaccacagccaagggaacgggcuuggcagaauca
gcggggcccuguugagcuuuuagucccucggaauuugaggcuagaaguua
ccacagggauaacuggcuuguggcagucaagcguucauagcgacauugcu
uuuugauugaugucggcucuuccuaucauaccgaagcagaauucgguaag
cguuggauuguucacccacuaauagggaagcuggaccgucgugagacagg
uuaguuuacccuacugaugaauguuaccgcaauaguaauugaacuuagua
cgagaggaacaguucauucggauaauugguuuuugcggcugucugaucag
gcauugccgcgaagcuaccauccgcuggauuauggcugaacgccucuaag
ucagaauccaugcuagaacgcggugauuucuuugcuccacacaauauaga
uggauacgaauaaggcguccuuguggcgucgcugaaccauagcaggcuag
caacggugcacuuggcggaaaggccuugggugcuugcuggcgaauugcaa
ugucauuuugcguggggauaaaucauuuguauacgacuuagauguacaac
gggguauuguaagcaguagaguagccuuguuguuacgaucugcugagauu
aagccuuuguugucugauuugu

..........................<<<<<<.....<<....>>.....
.>>>>>.>.........<<....>>..<<<<<......<<.....>>...
..>>>>>..<<<...........<<.<<<<<<...>>>>>>.>>......
.......<<<<<<.<<<<<<<<<<<<<<<<<<<<<<....<<<<......
>>>>.(.......<<<......)>>>..>.>>>>>>>>>..>>>>>>>..
..>>>>>>>>>>>........<<<<<<<.<....>.>>>>>>>.....<<
<<<..((((((>>>>>..>>>.............................
.....<<....>>...............<<<<....>>>>..........
........................<<<<<.<<<<<......<<<<....<
<..<<<<<<.<........>>>>>>>......>>....>>>>..<<<<<<
<.<<<<<<<<<<<<<<<.....<<<<<<<<<....<.<<<<<......>>
>>>>......>>>>>>>.>.>.>>>>>.>>>>>>>>>>........<<<.
...>>>.....>>>>>>>.....>>>>>.>>>>>..<<<<.<....>.>>
>><<<<<<....<<<<<<.<<<<<<..<<<...<<<<.....>>>>...>
>>...<<....>>.......<<<<<..<<<<<....>>>>>...>>>>>.
<<<<<<<<...<<....>>...>>>>.>>.>>....>>>>>>.>>>>>>.
.<<.<..<<<<<<<.....<<<<<<<<<..<<<....<<<<<<<....>>
>>>>>.....>>>...<<<<.<<.<<........>>>>>>>>....>>>>
>>>>>...<<.......>>......>>>>>>>.....>.>>..<<<<<<.
...<.<<......>>.><<<<<<<<<<...<<<.<<<<<<..........
...............>>>>>>>>>.>>>>>>>>.>>..<<<<.<<<<<..
...>>>>>.>>>>....<<<......>>>...<<<<<<<..<<<<(((..
...<<<<<<.....<<..........<<<.<<<<<<<<<<.......<<<
<<.<<<...<<.........>>...>>>.........<<......>>...
>>>>>...>>>>>>>>>>>>>..........))).>>...>>>>>>.>>>
>..>>>>>>><<<<<<<<<.....>>>>>>>>>....>>>>>><.<<<<<
<<<.<<......<<<<.<<<<....>>>>>>>>...>>>>>>>>>>..>.
....>>>>>><<.....<<.....<<<<<<.......>>>>>><<<<...
..<<<<<.<<...<<<<<<...<<.....>>.>>>>>>....((<<<<<<
...<<....<<<....>>>........<<<<<<........>>>>>>...
.>>....>>...<<<<<........>>>>>))<<<<.<<<<<<<<....<
<<....>>>...<...<<<<<<...<<<<.<<<<<<<<<<<<<<<<<<..
[[[..>>>>>><...<<<<<....<<<<<<....<<<.....>>>....>
>>>>>.....>>>>>....><<<<<<<<....>>>>>>>>>>>>.>.>>>
>>>>.>>>>.........>>>>>>...>.....>>>>..>>>>>>>>>>>
>..>>.....<<......>>>>>>>.<<<<.....>>>>..>>>>..<<<
<.....<<<<<<<.<<...............<<<<<..............
.>>>>><<.<<<<<<<<<<<..<<<<<<<.....................
............>>>>>>>.......>>>>>..>>>>>>.>>........
....<<<<<<<<<..<<<.........>>>..<<<<<<<....<<<<...
..>>>>......>>>>>>>....<..>....>>.>>>>>>>.....>>.>
>>>>>>..>>>>>>....>>....<<<<<........>>>>>....<<<<
<..<<<<<<<<<.<<<<<<<<<...<<<<.))))))>>>>.>>>>>>>>.
>.>>>>>>..............<<<<<...<<.......>>...>>>>>.
........((....<<<..<<<<<..<.<<<<<<<......>>>>>>>..
.>.<<<.....))..>>>.....>>>>>.>>>.<<<<<<<....>>>.>>
>>.......>>>.>>>>>....<<<<<<<<..<<<<.<.<<<<<<.....
..........>>>>>>.><<<<<...<<<<....<<<<<<<<<<]]].>>
>>>..>>>>>...>>>>..>>>>>.<<<<...<<<<<...........>>
>>>...>>>>.>>>>....>>>>>>>>.......<<<<<<.<<<.<<...
.<<..........<<<<<<.....>>>>>>......>>.......<<<<<
<...<<<<<.<..<<<......>>>..>.>>>>>.>>>>>>.........
....>>.>>>.>>>>>>..<<<<<<<.............>.....<<<<<
<<<<.<<<....<<<<.<..<<<<<......>>>>>..>>>>>...>>>.
...>>>>.>>>>>.>>>>>>..

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7u0h Sequence-specific remodeling of a topologically complex RNP substrate by Spb4.
• Resolution: 2.76 Å
• Binding residue (original residue number in PDB): R4 V5 T6 F7 R8 R9 R10 N11 P12 Y13 N14 T15 R16 S17 V22 K24 T25 P26 G27 L30 R31 K36 K37 L38 T40 P42 K43 Q52 G53 I54 S55 T56 R58 P59 R60 Y62 A63 T64 S66 K67 T68 K70 T71 S73 R74 Y76 G78 N83
• Binding residue (residue number reindexed from 1): R3 V4 T5 F6 R7 R8 R9 N10 P11 Y12 N13 T14 R15 S16 V21 K23 T24 P25 G26 L29 R30 K35 K36 L37 T39 P41 K42 Q51 G52 I53 S54 T55 R57 P58 R59 Y61 A62 T63 S65 K66 T67 K69 T70 S72 R73 Y75 G77 N82

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0002181 cytoplasmic translation
• GO:0006412 translation
• GO:0042254 ribosome biogenesis

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:0030687 preribosome, large subunit precursor
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:7u0h, PDBe:7u0h, PDBj:7u0h
• PDBsum: 7u0h
• PubMed: 36482249
• UniProt: P87262|RL34A_YEAST Large ribosomal subunit protein eL34A (Gene Name=RPL34A)