Structure of PDB 7ase Chain g Binding Site BS01

Receptor Information

>7ase Chain g (length=83) Species: 5693 (Trypanosoma cruzi)

IGTYNEEGVNVDLYIPRKCHATNNLITSYDHSAVQIAIANVDANGVLNGT
TTTFCIAGYLRRQAESDHAINHLAISKGIIRIK

Ligand information

>7ase Chain 0 (length=2150)

gaucugguugauucugccaguagucauaugcuuguuucaaggacuuagcc
augcaugccucagaaucacugcauugcaggaaucugcgcauggcucauua
caucagacguaaucugccgcaaaaaucuugcggucuccgcaacauuggau
aacuuggcgaaacgccaagcuaauacaugaaccaaccggauguucucugu
uccggcggcagggcaaccugcugccaugggacguccagcgaaugaaugaa
aguaaaaccaaugccuucaccggcaguaacacucagaaguguugauucaa
uucauuccgugcgaaagccggguuuuuuuauccggcgucuuuugacgaac
aacugcccuaucagccagcgauggccguguaguggacugccauggcguug
acgggagcgggggauuaggguucgauuccggagagggagccugagaaaua
gcuaccacuucuacggagggcagcaggcgcgcaaauugcccaaugucaaa
aaaaaaagaugaggcagcgaaaagaaauagagccgacagugcuuuugcau
ugucguuuucaaugggggauauuuaaacccauccaaaaucgaguaacaau
uggaggacaagucuggugccagcacccgcgguaauuccagcuccaaaagc
guauauuaaugcuguugcuguuaaaggguucguaguugaauugagggccu
cuaaggcgcaaugguuuagucccauccacuucggauuggugacccaugcc
cuugugguccgugaacagacauucagaaacaaaaaacacgggagugguac
cuuuccugauuaucgcaugucaugcaugccagagggcgcccgugauuuuu
uacugugacuaaaaaagugugaccaaagcagucauucgacuugaauuaga
aagcaugggauaacaaaggagcagccucugggccaccguuucggcuuuug
uugguuuuaaaaguccauuggagauuauggggcagugugacaagcggcug
ggugcacucgucgccuuugcaacucacggcauccaggaaugaaggagggu
aguucgggggagaacguacuggugcgucagaggugaaauucuuagaccgc
accaagacgaacuacagcgaaggcauucuucaaggauaccuuccucaauc
aagaaccaaaguguggggaucgaagaugauuagagaccauuguaguccac
acugcaaacgaugacacccaugaauuggggaguuuuuggucguaggcgug
gucgggcuugauuauuauuuuucaucccguuccucgucucgccaaugaau
auuaaauuuacgugcauauucuuuuuggucuucguuuuuuuacggcgagg
gccuuuaacgggaauauccucagcacguuaucugacuucuucacgcgaaa
gcuuugagguuacagucucaggggggaguacguucgcaagagugaaacuu
aaagaaauugacggaauggcaccacaagacguggagcgugcgguuuaauu
ugacucaacacggggaacuuuaccagauccggacagggugaggauugaca
gauugaguguucuuucucgauccccugaaugguggugcauggccgcuuuu
ggucgguggagugauuuguuugguugauuccgucaacggacgagauccaa
gcugcccaguaggauucagaauugcccccuuccgcggguuuuacccaagg
gggggcgguauucgcuuguauccuucucugcgggauuccuuguuuugcgc
aaggugagauuuugggcaacagcaggucugugaugcuccucaauguucug
ggcgacacgcgcacuacaaugucagugagaacaagaaaaacgacucuugu
cggaccuacuugaucaaaagagugggaaaaccccggaaucacguagaccc
acuugggaccgaguauugcaauuauuggucgcgcaacgaggaaugucucg
uaggcgcagcucaucaaacugugccgauuacgucccugccauuuguacac
accgcccgucguuguuuccgaugauggugcaauacaggugaucggacagu
cgagugcuucacuugaccgaaaguucaccgauauuucuucaauagaggaa
gcaaaagucguaacaagguagcuguaggugaaccugcagcuggaucauuu

...<<<<<...{{{{>>>>>{{{{.[[[[[[...[[[..........[[[
.[[[..<<....<<....<<<<...>>>>...>>.>>......{{.....
...[[[..[[..[[...[[.[[.....<<<<<<...>>>>>>...<<...
..<<<<<<.....>>>>>>......>>..........<<<<<.<<<....
...<<<<<<<<<....>>>>>>>>>...>>>>>>>>.[[((((((((...
...<<<<<<<<<<<<......>>>.........................)
)))))))...]]....<<<<<<........>>>>>>...]].]]..]]]]
..]]].<<<....<<<....<<<<<.<<.......>>.>>>>>>>>....
..>>>..<<<<<<<<....>>>>...>>>>.}}.<<.<<<..........
>>>.>>.<<<<....>>>>...]]]]]].........<<<....<<<...
.......>>>..>>>.......]]]....<<<.<<<<<<<<<....>>>>
>>>>>..>>>.....<<<<............>>>.....>.........<
<<<<((....<.<<<<.....<<..))>>.......>>>>>>>>>>..]]
]]]]}}}}.........{{{{{.....[[[[...[[[.......<<<<<.
<.<<<<.<<<..<<............................>>..>>>>
>>>>.>>>>>..........................<<<<<<..<....<
<<<.<.........<<<<.......>>>>..>>>>>.>>>>>>>.....<
<<<<<<<.......<<...<.......>..<<<....>>>.....>>...
...>>>>>..>>>...........[[..(((((..<<<...>>>.((..>
>>>>>>>>))..)))))..]].......<<<<<...<<<....<<<<<..
<<<..>>>.>>>>>..<<<>>>..>>>.>>.>>>............<<<<
<..<<...<<<<<<<<..<<<<<<<<...<<<......>>>......>>>
>>>>.>.>>.......<<....>>.>>>>>>..>>.>>>.>>....]]].
..]]]]...<<<<<<<<...<...<<<<.<.....>.>>>>...>>>>>>
>>>.........<<<.<<...<..<<<<<.......<<<.<<.<<<<<.<
<..<<<...<............>..>>>...>>.>>>>>>>>>>......
.....................<.<<...<<<<<<<.........>>>>>>
>.........>>.>................>>>>>..>......<<..}}
>>.....>>.....>>>.}}}.<<<......<<<<....>>>>....>>>
..}}}}..<<<<<.<<<<<<<..<<.<<<<<<.....<<<<<<.......
<<........>>.......<..<<.<<......<.<<<........<<.<
<<........>>>.>>......>>>.>.<.<<.<<<..<<.<<<<<<...
.<<<.<<<<......>>...<<<......>>>...>>.>>>....<<<<<
.<<<<.....<<<<<.<<..<.....<<<<<<<..<<<<...>>>>..>>
>>>>>......>....>>.>>>>>.....>>>>...<<.<.<<.....>>
>.>>.......>>>>>....>>>.>>>.....>>>>>>>>......>.>.
>>.>...>.>>>>>....................................
....<<<<<<<........>>>>>>>........................
....<....<<<.<<..........>>.>>>.....>.....>>>>>>..
..<<<<<<<.........>>>>>>>......>>...>>>>>>>>>>.>>.
...<..<<.<.<<<<<<<<..<<.<<<.<.<<<<..<<<<<.<...<.<<
<<<<<<...>>>>>>>>.>...>.>>>>>..>>>>.>.>>>.>>..>>>>
>>>>..>.>>...>.....<<<<<<<<<....>>>>>>>>>.........

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7ase Structural Differences in Translation Initiation between Pathogenic Trypanosomatids and Their Mammalian Hosts.
• Resolution: 3.33 Å
• Binding residue (original residue number in PDB): R65 H75 I78 S79 R84 I85 K86
• Binding residue (residue number reindexed from 1): R62 H72 I75 S76 R81 I82 K83

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0000447 endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
• GO:0000461 endonucleolytic cleavage to generate mature 3'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
• GO:0006412 translation

Cellular Component

• GO:0005840 ribosome
• GO:0022627 cytosolic small ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:7ase, PDBe:7ase, PDBj:7ase
• PDBsum: 7ase
• PubMed: 33357443
• UniProt: A0A2V2UKQ3