Structure of PDB 6yef Chain g Binding Site BS01

Receptor Information

>6yef Chain g (length=146) Species: 93061 (Staphylococcus aureus subsp. aureus NCTC 8325)

VPKRDVLPDPIHNSKLVTKLINKIMLDGKRGTAQRILYSAFDLVEQRSGR
DALEVFEEAINNIMPVLEVKARRSNYQVPVEVRPERRTTLGLRWLVNYAR
LRGEKTMEDRLANEILDAANNTGGAVKKREDTHKMAEANKAFAHYR

Ligand information

>6yef Chain a (length=1545)

uggagaguuugauccuggcucaggaugaacgcuggcggcgugccuaauac
augcaagucgagcgaacggacgagaagcuugcuucucugauguuagcggc
ggacgggugaguaacacguggauaaccuaccuauaagacugggauaacuu
cgggaaaccggagcuaauaccggauaauauuuugaaccgcaugguucaaa
agugaaagacggucuugcugucacuuauagauggauccgcgcugcauuag
cuaguugguaagguaacggcuuaccaaggcaacgaugcauagccgaccug
agagggugaucggccacacuggaacugagacacgguccagacuccuacgg
gaggcagcaguagggaaucuuccgcaaugggcgaaagccugacggagcaa
cgccgcgugagugaugaaggucuucggaucguaaaacucuguuauuaggg
aagaacauauguguaaguaacugugcacaucuugacgguaccuaaucaga
aagccacggcuaacuacgugccagcagccgcgguaauacguagguggcaa
gcguuauccggaauuauugggcguaaagcgcgcguaggcgguuuuuuaag
ucugaugugaaagcccacggcucaaccguggagggucauuggaaacugga
aaacuugagugcagaagaggaaaguggaauuccauguguagcggugaaau
gcgcagagauauggaggaacaccaguggcgaaggcgacuuucuggucugu
aacugacgcugaugugcgaaagcguggggaucaaacaggauuagauaccc
ugguaguccacgccguaaacgaugagugcuaaguguuaggggguuuccgc
cccuuagugcugcagcuaacgcauuaagcacuccgccuggggaguacgac
cgcaagguugaaacucaaaggaauugacggggacccgcacaagcggugga
gcaugugguuuaauucgaagcaacgcgaagaaccuuaccaaaucuugaca
uccuuugacaacucuagagauagagccuuccccuucgggggacaaaguga
cagguggugcaugguugucgucagcucgugucgugagauguuggguuaag
ucccgcaacgagcgcaacccuuaagcuuaguugccaucauuaaguugggc
acucuaaguugacugccggugacaaaccggaggaagguggggaugacguc
aaaucaucaugccccuuaugauuugggcuacacacgugcuacaauggaca
auacaaagggcagcgaaaccgcgaggucaagcaaaucccauaaaguuguu
cucaguucggauuguagucugcaacucgacuacaugaagcuggaaucgcu
aguaaucguagaucagcaugcuacggugaauacguucccgggucuuguac
acaccgcccgucacaccacgagaguuuguaacacccgaagccgguggagu
aaccuuuuaggagcuagccgucgaaggugggacaaaugauuggggugaag
ucguaacaagguagccguaucggaaggugcggcuggaucaccucc

....<<<<..[.((((.>>>>.<<<<.<<<<<..<<<<<<<<.....<<<
.<<<..<<<..<<.<<<<....<<..........>>....>>>>.>>>>>
......<<........<<<<<<<..<<...<<<<<<<.<<<<.....<<<
<<<....>>>>>>......>>>>......<<<<<<<<<....>>>>>>>>
>......<<<<<<...>>>>>>.>>>>>>>..>>>>>>>>>.<<<....<
<<..<<<<<<<<.......>>>>>>>>>>>......>>>..<<<<<<<<.
...>>>>...>>>>.>>.<<<<<.<.........>>>>>>.<<<<<..>>
>>>...>>>>>>.........<<<....<<<<....>>>>..>>>..>>.
>>>>>>..<<<<.......<<<....>>>......>>>>....<<<<<<.
........................................>>>>>>....
..<<<<<(((...<<<<<.....<<.)))>>.......>>>>>>>>>>..
>>>>>>>>>..........<<<((.....<<<<<..<<<.<<<<<<<.<<
<<<<<<<.......<<<<<<.....>>>>>>.....>>>>>>>..>>>>>
>>>>...<<<<<<<<...<<<<<<<....<<<<<<<<.<.<<<......>
>>.....>>>>>>>>>...........<<....>>.>>>>>>>..>>>>>
.>>>...>>>..>>>>>....<<<<<<<...<...<<<<.<.....>.>>
>>...>>>>>>>>..........<<<<<<..<<<<<<<<<<<<<....>>
>>>>>>>>>>>...<<..))>>.....>>>>>>.>>>.<<<......<<<
<....>>>>....>>>..)))).]<<<<<.<<<<<<<.<<.<<<<<<..<
<<<<<<<<<......<<........>>..........<<<<<<<......
<.<<<......<<<<<....>>>>>.....<<<<..>>>>...>>.>.>.
.<<<.<<<..<<<<<<.......<<<<<<<<<....>>>..<<<<.....
.>>>>..>>>>>>.....<<<<.<<<<<<<..<<..<<<......>>>>>
...>>>>>>>.....<<<<<.....>>>>>........>>>>........
.>>>...>>>>>>>>>...>>>>>>>...>>.>>>>>>>>.....<<<<<
<<.....<<<..<<...<<<....>>>...>>....>>>.....>>>>>>
>......<....<<<<<<<........>>>>>>>....>.....>>>>>>
....<<<<<<<.........>>>>>>>......>>...>>>>>>>>>>.>
>.......<<.<.<<<<.<<<..<<<<<<..<<<<....<.<<<<..<<<
..<<<...>>>.>>>.>>>>.>...>>>>..>>>>>>..>>>.>>>>..>
.>>.........<<<<<<<<<....>>>>>>>>>...........

Receptor-Ligand Complex Structure

Structure summary

• PDB: 6yef Cryo-EM structure of the ribosome functional complex of the human pathogen Staphylococcus aureus at 3.2 angstrom resolution.
• Resolution: 3.2 Å
• Binding residue (original residue number in PDB): K9 R10 N28 I30 M31 D33 G34 K35 R36 G37 T38 R95 T98 R102 R109 K114 M116
• Binding residue (residue number reindexed from 1): K3 R4 N22 I24 M25 D27 G28 K29 R30 G31 T32 R86 T89 R93 R100 K105 M107

Gene Ontology

Molecular Function

• GO:0000049 tRNA binding
• GO:0003723 RNA binding
• GO:0003729 mRNA binding
• GO:0003735 structural constituent of ribosome
• GO:0019843 rRNA binding

Biological Process

• GO:0000028 ribosomal small subunit assembly
• GO:0006412 translation

Cellular Component

• GO:0005840 ribosome
• GO:0015935 small ribosomal subunit
• GO:0022627 cytosolic small ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:6yef, PDBe:6yef, PDBj:6yef
• PDBsum: 6yef
• PubMed: 32852796
• UniProt: P48940|RS7_STAA8 Small ribosomal subunit protein uS7 (Gene Name=rpsG)