Structure of PDB 8wib Chain f Binding Site BS01

Receptor Information

>8wib Chain f (length=166) Species: 246196 (Mycolicibacterium smegmatis MC2 155)

NYIERVVSINRVSKVVKGGRRFSFTALVIVGDGKGMVGVGYGKAKEVPAA
IAKGVEEARKNFFRVPLIGSTITHPVQGEAAAGVVMLRPASPGTGVIAGG
AARAVLECAGVHDILAKSLGSDNAINVVHATVAALKLLQRPEEVAARRGL
PIEDVAPAGMLKARRE

Ligand information

>8wib Chain a (length=1515)

uuggagaguuugauccuggcucaggacgaacgcuggcggcgugcuuaaca
caugcaagucgaacggaaaggcccuuucggggguacucgaguggcgaacg
ggugaguaacacgugggugaucugcccugcacuuugggauaagccuggga
aacugggucuaauaccgaauacacccugcuggucgcauggccugguaggg
gaaagcuuuugcggugugggaugggcccgcggccuaucagcuuguuggug
gggugauggccuaccaaggcgacgacggguagccggccugagagggugac
cggccacacugggacugagauacggcccagacuccuacgggaggcagcag
uggggaauauugcacaaugggcgcaagccugaugcagcgacgccgcguga
gggaugacggccuucggguuguaaaccucuuucagcacagacgaagcgca
agugacgguaugugcagaagaaggaccggccaacuacgugccagcagccg
cgguaauacguaggguccgagcguuguccggaauuacugggcguaaagag
cucguaggugguuugucgcguuguucgugaaaacucacagcuuaacugug
ggcgugcgggcgauacgggcagacuagaguacugcaggggagacuggaau
uccugguguagcgguggaaugcgcagauaucaggaggaacaccgguggcg
aaggcgggucucugggcaguaacugacgcugaggagcgaaagcgugggga
gcgaacaggauuagauacccugguaguccacgccguaaacgguggguacu
agguguggguuuccuuccuugggauccgugccguagcuaacgcauuaagu
accccgccuggggaguacggccgcaaggcuaaaacucaaaggaauugacg
ggggcccgcacaagcggcggagcauguggauuaauucgaugcaacgcgaa
gaaccuuaccuggguuugacaugcacaggacgccggcagagaugucgguu
cccuuguggccugugugcagguggugcauggcugucgucagcucgugucg
ugagauguuggguuaagucccgcaacgagcgcaacccuugucucauguug
ccagcacguuaugguggggacucgugagagacugccggggucaacucgga
ggaagguggggaugacgucaagucaucaugccccuuauguccagggcuuc
acacaugcuacaauggccgguacaaagggcugcgaugccgugagguggag
cgaauccuuucaaagccggucucaguucggaucggggucugcaacucgac
cccgugaagucggagucgcuaguaaucgcagaucagcaacgcugcgguga
auacguucccgggccuuguacacaccgcccgucacgucaugaaagucggu
aacacccgaagccgguggccuaacccuuguggagggagccgucgaaggug
ggaucggcgauugggacgaagucguaacaagguagccguaccggaaggug
cggcuggaucaccuc

.....<<<<..[.((((.>>>>.<<<<.<<<<<..<<<<<<<<.....<<
<.<<<..<<<..<<.<..<<<<<<<....>>>>>.>>>..>>>>>.....
.<<........<<<<<<<..<<...<<<<<<<<<<<<.....<<<<<<..
..>>>>>>......>>>>.....<<<<<<<<<<<<..>>>>>.>>>>>>>
....<<....>>>>>>>>>>..>>>>>>>>>.<<<....<<<..<<<<<<
<<.......>>>>>>>>>>>......>>>..<<<<<<<<....>>>>...
>>>>.>>.<<<<<.<.........>>>>>>.<<<<....>>>>...>>>>
>>.........<<<....<<<<....>>>>..>>>..>>.>>>>>>..<<
<<......<<<<....>>>>.....>>>>...<.<<<<<......<<...
.>>.......>>>>>....>..<<<<<(((...<<<<<.....<<.)))>
>.......>>>>>>>>>>..>>>>>>>>>..........<<<((.....<
<<<...<<<.<<<<<<<.<<<<<<<<<<.....<<<<<<<.....>>>>>
>>...>>>>>>>..>>>>>>>>>>...<<<<<<<<...<<<<<<<....<
<<<<<<<<..<<<......>>>.....>>>>>>>>>...........<<.
...>>.>>>>>>>..>>>>>.>>>...>>>...>>>>....<<<<<<...
<<...<<<<.<.....>.>>>>...>>>>>>>>..........<<<<<<.
.<<<<<<<<<<<<.......>>>>>>>>>>>>...<<..))>>.....>>
>>>>.>>>.<<<......<<<<....>>>>....>>>..)))).]<<<<<
.<<<<<<<.<<.<<<<<<..<<<<<<<<<<......<<........>>..
........<<<<<<<......<<<<<<<...<<<<<<<....>>>>>>>.
<<.....>>.>>>>>>>.<<<.<<<..<<<<<<.......<<<<<<<<<.
...>>>..<<<<......>>>>..>>>>>>.....<<<<.<<<<<<....
<<..<<<......>>>>>.....>>>>>>.....<<<<<.....>>>>>.
.......>>>>.........>>>...>>>>>>>>>...>>>>>>>...>>
.>>>>>>>>.....<<<<<<<.....<<<..<<..<<<<....>>>>..>
>....>>>......>>>>>>>......<....<<<<<<<........>>>
>>>>....>.....>>>>>>....<<<<<<<..........>>>>>>>..
....>>...>>>>>>>>>>.>>....<..<<.<.<<<<.<<<..<<<<<<
..<<<<....<.<<<<..<<<..<<.....>>.>>>.>>>>.>...>>>>
..>>>>>>..>>>.>>>>..>.>>...>.....<<<<<<<<<....>>>>
>>>>>..........

Receptor-Ligand Complex Structure

Structure summary

• PDB: 8wib Cryo- EM structure of the mycobacterial 70S ribosome in complex with ribosome hibernation promotion factor RafH.
• Resolution: 3.5 Å
• Binding residue (original residue number in PDB): N44 S47 V49 K51 G52 R54 R55 Y75 K77 K87 M120 R122 A124 S125 T128 I131 A132 G133 R137 L149 K151 S155 D156 N157 N160
• Binding residue (residue number reindexed from 1): N10 S13 V15 K17 G18 R20 R21 Y41 K43 K53 M86 R88 A90 S91 T94 I97 A98 G99 R103 L115 K117 S121 D122 N123 N126

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003735 structural constituent of ribosome
• GO:0019843 rRNA binding

Biological Process

• GO:0006412 translation

Cellular Component

• GO:0005737 cytoplasm
• GO:0005840 ribosome
• GO:0015935 small ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:8wib, PDBe:8wib, PDBj:8wib
• PDBsum: 8wib
• PubMed: 38245551
• UniProt: A0QSG6|RS5_MYCS2 Small ribosomal subunit protein uS5 (Gene Name=rpsE)